Background： Plasmodium falciparum malaria remains a major life-threatening disease. Recently, the Plasmodium apoptosis-linked pathogenicity factors （PALPF） have been identified. These antigens PALPF are expressed o...Background： Plasmodium falciparum malaria remains a major life-threatening disease. Recently, the Plasmodium apoptosis-linked pathogenicity factors （PALPF） have been identified. These antigens PALPF are expressed only by P falciparum-infected erythrocytes triggering endothelial cell apoptosis （apoptogenic）. Methods： We designed ten synthetic peptides （PI to P10） from PALPF： PF07 0032, PF10_0226, PFI0130c, PFD0875c and MAL13P1.206, and analyzed their antigenicity with an ELISA method using plasma samples from subjects living in Dienga, Gabon. Results： Four peptides showed good reactivity with human antibodies. The prevalence rate of specific IgG was 61%, 51%, 44% and 34% for P5, P6, P4 and P2, respectively. The median optical density of total IgG anti-P2 was higher than that directed against P4 and P6 （P = 0.009; P = 0.012 respectively）. The prevalence rate oflgG subclasses determined with plasma samples recognizing peptide 5 for IgGl, 2, 3 and 4 isotypes was 69%, 45%, 76% and 62%, respectively. All the subjects had at least one immunoglobulin subclass, while 13 （44%） had both IgG1 and IgG3 antibodies. There was no significant difference in the prevalence rate of anti-P5 IgG1, IgG3 and IgG4. Conclusion： These results warrant further immunogenicity studies of peptides 2, 4, 5 and 6 with a view of a tentative to antimalarial vaccine development.展开更多
Two new species of Protura are described:Baculentulus wenyingae sp.nov.and Pseudanisentomon lishuiensis sp.nov.Baculentulus wenyingae sp.nov.is characterized by the combined characters of the presence of foretarsal se...Two new species of Protura are described:Baculentulus wenyingae sp.nov.and Pseudanisentomon lishuiensis sp.nov.Baculentulus wenyingae sp.nov.is characterized by the combined characters of the presence of foretarsal sensillum b',differentiated sensilla on the maxillary palp,leaf-shaped sensillum on the labial palp,absence of P1a seta on tergites I–VI,eight anterior setae on tergite VI and 4/18 chaetotaxy on tergite VII.An updated key to the 13 Baculentulus species from China is also provided.Pseudanisentomon lishuiensis sp.nov.is characterized by the absence of foretarsal sensillum b'-2,b longer than c,the empodial appendage of II and III leg very short,the tergite I with 12 posterior setae,tergites V–VII lack seta A3,and sternites VIII–X with 2/7-4-4 chaetotaxy respectively.It was compared with the affiliated species P.guangxiensis(Yin&Zhang,1982)in detail.In addition,a checklist and the distribution of 39 Protura species from Zhejiang Province are provided,and the Protura fauna in Zhejiang is also discussed.展开更多
文摘Background： Plasmodium falciparum malaria remains a major life-threatening disease. Recently, the Plasmodium apoptosis-linked pathogenicity factors （PALPF） have been identified. These antigens PALPF are expressed only by P falciparum-infected erythrocytes triggering endothelial cell apoptosis （apoptogenic）. Methods： We designed ten synthetic peptides （PI to P10） from PALPF： PF07 0032, PF10_0226, PFI0130c, PFD0875c and MAL13P1.206, and analyzed their antigenicity with an ELISA method using plasma samples from subjects living in Dienga, Gabon. Results： Four peptides showed good reactivity with human antibodies. The prevalence rate of specific IgG was 61%, 51%, 44% and 34% for P5, P6, P4 and P2, respectively. The median optical density of total IgG anti-P2 was higher than that directed against P4 and P6 （P = 0.009; P = 0.012 respectively）. The prevalence rate oflgG subclasses determined with plasma samples recognizing peptide 5 for IgGl, 2, 3 and 4 isotypes was 69%, 45%, 76% and 62%, respectively. All the subjects had at least one immunoglobulin subclass, while 13 （44%） had both IgG1 and IgG3 antibodies. There was no significant difference in the prevalence rate of anti-P5 IgG1, IgG3 and IgG4. Conclusion： These results warrant further immunogenicity studies of peptides 2, 4, 5 and 6 with a view of a tentative to antimalarial vaccine development.
基金supported by the National Natural Science Foundation of China (31772509, 31970438, 31471958, 31772510)the Basic Research Foundation of Shanghai Science & Technology Museum
文摘Two new species of Protura are described:Baculentulus wenyingae sp.nov.and Pseudanisentomon lishuiensis sp.nov.Baculentulus wenyingae sp.nov.is characterized by the combined characters of the presence of foretarsal sensillum b',differentiated sensilla on the maxillary palp,leaf-shaped sensillum on the labial palp,absence of P1a seta on tergites I–VI,eight anterior setae on tergite VI and 4/18 chaetotaxy on tergite VII.An updated key to the 13 Baculentulus species from China is also provided.Pseudanisentomon lishuiensis sp.nov.is characterized by the absence of foretarsal sensillum b'-2,b longer than c,the empodial appendage of II and III leg very short,the tergite I with 12 posterior setae,tergites V–VII lack seta A3,and sternites VIII–X with 2/7-4-4 chaetotaxy respectively.It was compared with the affiliated species P.guangxiensis(Yin&Zhang,1982)in detail.In addition,a checklist and the distribution of 39 Protura species from Zhejiang Province are provided,and the Protura fauna in Zhejiang is also discussed.
