Sertoli cells play essential roles in the process of spermatogenesis such as maintaining the integrity of blood-testis barrier(BTB),engulfing sperm resid ual cytoplasm and secreting critical cytokines.PA1 has been rep...Sertoli cells play essential roles in the process of spermatogenesis such as maintaining the integrity of blood-testis barrier(BTB),engulfing sperm resid ual cytoplasm and secreting critical cytokines.PA1 has been reported to be a unique component of histone lysine methylation modification complex MLL3/4,regulating gene transcription by histone 3 lysine 4 methylation(H3K4me).However,it is unknown whether PA1 is involved in the epigenetic regulation in male reproductive system.展开更多
利用海藻酸在pH2.7的条件下对小分子多肽的吸附作用,从豌豆种子中分离并纯化出含37个氨基酸的小分子肽PA1b(pea albumin 1b),它的肽链内具有6个半胱氨酸并形成一个胱氨酸结构模体.采用荧光显微技术和膜片钳技术,发现胞外施加PA1b在胞外...利用海藻酸在pH2.7的条件下对小分子多肽的吸附作用,从豌豆种子中分离并纯化出含37个氨基酸的小分子肽PA1b(pea albumin 1b),它的肽链内具有6个半胱氨酸并形成一个胱氨酸结构模体.采用荧光显微技术和膜片钳技术,发现胞外施加PA1b在胞外钙离子存在的情况下使胰腺β细胞内钙离浓度增加,该效应被特异性的L型钙通道的阻断剂尼莫地平(nimodipine)阻断,在零钙外液中PA1b对胞内钙离子浓度无影响;此外,PA1b使β细胞膜去极化并使膜电容增加.因此推断PA1b使原代β细胞上去极化细胞膜,使L型钙离子通道开放,细胞外钙离子内流并促发细胞分泌.展开更多
Using alginic acid to adsorb polypeptides at pH 2.7,we isolated a peptide pea albumin 1b(PA1b)from pea seeds.The PA1b is a single chain peptide consisting of 37 amino acid residues with 6 cysteines which constitutes t...Using alginic acid to adsorb polypeptides at pH 2.7,we isolated a peptide pea albumin 1b(PA1b)from pea seeds.The PA1b is a single chain peptide consisting of 37 amino acid residues with 6 cysteines which constitutes the cystine-knot structure.Using microfluorometry and patch clamp techniques,we found that PA1b significantly elevated the intracellular calcium level([Ca2+]i)and elicited membrane capacitance increase in the primary rat pancreaticβcells.The PA1b effect on[Ca2+]i elevation was abolished in the absence of extracellular Ca2+or in the presence of L-type Ca2+channel blocker,ni-modipine.Interestingly,we found that PA1b significantly depolarized membrane potential,which could lead to the opening of voltage-dependent L-type Ca2+channels and influx of extracellular Ca2+,and then evoke robust secretion.In this study we identified the plant peptide PA1b which is capable of affecting the excitability and function of mammalian pancreaticβcell.展开更多
There is an error in the title of the published paper "PA1426 regulates Pseudomonas aeruginosa quorum sensing and virulence: an in vitro study" (DOI: 10.1097/JBR.0000000000000088) because of a mistake in des...There is an error in the title of the published paper "PA1426 regulates Pseudomonas aeruginosa quorum sensing and virulence: an in vitro study" (DOI: 10.1097/JBR.0000000000000088) because of a mistake in description and also oversight during final proof checking.[1] The correct one should be“PA2146 regulates Pseudomonas aeruginosa quorum sensing and virulence:an in vitro study.”展开更多
目的探索PTIP相关蛋白1(PTIP associated protein 1,PA1)在小鼠睾丸发育过程中的表达定位。方法采用RTPCR、实时定量PCR和免疫组织化学方法,对PA1在小鼠睾丸不同发育阶段的表达及定位进行检测。结果 RT-PCR和实时定量PCR结果显示,PA1 m ...目的探索PTIP相关蛋白1(PTIP associated protein 1,PA1)在小鼠睾丸发育过程中的表达定位。方法采用RTPCR、实时定量PCR和免疫组织化学方法,对PA1在小鼠睾丸不同发育阶段的表达及定位进行检测。结果 RT-PCR和实时定量PCR结果显示,PA1 m RNA在1w、2w、4w、8w、12w、18w和24w的小鼠睾丸中均有表达,且其表达量在2w时达到最高峰,在小鼠性成熟(8w)以后,PA1的表达量趋于平稳。ABC法免疫组织化学染色显示PA1在1w、2w、4w和8w小鼠睾丸各级生精细胞、支持细胞和间质细胞的胞核中均有表达,免疫荧光双标进一步确定PA1表达于支持细胞和间质细胞。结论 PA1可能在维持生精细胞的正常分化及调节睾丸内分泌的平衡中起重要作用。展开更多
文摘Sertoli cells play essential roles in the process of spermatogenesis such as maintaining the integrity of blood-testis barrier(BTB),engulfing sperm resid ual cytoplasm and secreting critical cytokines.PA1 has been reported to be a unique component of histone lysine methylation modification complex MLL3/4,regulating gene transcription by histone 3 lysine 4 methylation(H3K4me).However,it is unknown whether PA1 is involved in the epigenetic regulation in male reproductive system.
文摘利用海藻酸在pH2.7的条件下对小分子多肽的吸附作用,从豌豆种子中分离并纯化出含37个氨基酸的小分子肽PA1b(pea albumin 1b),它的肽链内具有6个半胱氨酸并形成一个胱氨酸结构模体.采用荧光显微技术和膜片钳技术,发现胞外施加PA1b在胞外钙离子存在的情况下使胰腺β细胞内钙离浓度增加,该效应被特异性的L型钙通道的阻断剂尼莫地平(nimodipine)阻断,在零钙外液中PA1b对胞内钙离子浓度无影响;此外,PA1b使β细胞膜去极化并使膜电容增加.因此推断PA1b使原代β细胞上去极化细胞膜,使L型钙离子通道开放,细胞外钙离子内流并促发细胞分泌.
基金the National Natural Science Foundation of China(Grant Nos.30370674,30470448,and 30470646)the CAS Project(Grant No.KSCX2-SW-224)+1 种基金the China"863"Program(Grant No.2012AA214066)The laboratory of Tao Xu is also supported by the Partner Group Scheme of the Max Planck Institute for Bio-physical Chemistry,Gttingen
文摘Using alginic acid to adsorb polypeptides at pH 2.7,we isolated a peptide pea albumin 1b(PA1b)from pea seeds.The PA1b is a single chain peptide consisting of 37 amino acid residues with 6 cysteines which constitutes the cystine-knot structure.Using microfluorometry and patch clamp techniques,we found that PA1b significantly elevated the intracellular calcium level([Ca2+]i)and elicited membrane capacitance increase in the primary rat pancreaticβcells.The PA1b effect on[Ca2+]i elevation was abolished in the absence of extracellular Ca2+or in the presence of L-type Ca2+channel blocker,ni-modipine.Interestingly,we found that PA1b significantly depolarized membrane potential,which could lead to the opening of voltage-dependent L-type Ca2+channels and influx of extracellular Ca2+,and then evoke robust secretion.In this study we identified the plant peptide PA1b which is capable of affecting the excitability and function of mammalian pancreaticβcell.
文摘There is an error in the title of the published paper "PA1426 regulates Pseudomonas aeruginosa quorum sensing and virulence: an in vitro study" (DOI: 10.1097/JBR.0000000000000088) because of a mistake in description and also oversight during final proof checking.[1] The correct one should be“PA2146 regulates Pseudomonas aeruginosa quorum sensing and virulence:an in vitro study.”
文摘目的探索PTIP相关蛋白1(PTIP associated protein 1,PA1)在小鼠睾丸发育过程中的表达定位。方法采用RTPCR、实时定量PCR和免疫组织化学方法,对PA1在小鼠睾丸不同发育阶段的表达及定位进行检测。结果 RT-PCR和实时定量PCR结果显示,PA1 m RNA在1w、2w、4w、8w、12w、18w和24w的小鼠睾丸中均有表达,且其表达量在2w时达到最高峰,在小鼠性成熟(8w)以后,PA1的表达量趋于平稳。ABC法免疫组织化学染色显示PA1在1w、2w、4w和8w小鼠睾丸各级生精细胞、支持细胞和间质细胞的胞核中均有表达,免疫荧光双标进一步确定PA1表达于支持细胞和间质细胞。结论 PA1可能在维持生精细胞的正常分化及调节睾丸内分泌的平衡中起重要作用。
