Objective: To compare the intraoperative signal-to-noise ratio (SNR), reproducibility and rapidity of popliteal fossa (PF), optimized P37, standard P37 and P31 potentials. Methods: Raw sweeps and 11 averages doubling ...Objective: To compare the intraoperative signal-to-noise ratio (SNR), reproducibility and rapidity of popliteal fossa (PF), optimized P37, standard P37 and P31 potentials. Methods: Raw sweeps and 11 averages doubling sweep number from 2 to 2048 were compared in 37 patients undergoing scoliosis surgery. Optimized (highest amplitude or SNR) P37 derivations were Cz-CPc (22), CPz-CPc (27), Pz-CPc (7), iCPi-CPc (8), CPi-CPc (1), Cz-Pz (2)orPz-FPz (3), and in two patients with non-decussation, Cz-CPi (1) or CPz-CPi (3). Standard P37 and P31 derivations were CPz-FPz and FPz-C5S. Signal amplitude was measured in 2048 sweep averages; peak noise was measured in raw sweeps and ±averages; SNR was amplitude/noise. Visual superimposability and < 20-30%amplitude variation determined reproducibility. Sweeps to reproducibility determined rapidity. Results: The SNR order was PFoptimized P37 > standard P37 > P31. Mean optimized P37 SNR advantages over the standard P37 and P31 were 2.1:1 and 4.9:1. SNR had powerful non-linear correlations to reproducibility and rapidity. Median sweeps to reproducibility were PF: 2, optimized P37: 128, standard P37: 512 and P31: 1024. EEG noise was greatest in FPz derivations. Burst-suppression increased scalp potential SNR and rapidity. Conclusions: Optimized P37 and PF recordings are most rapidly reproducible due to superior SNRs and are recommended. FPz should be avoided. Burst-suppression may be desirable. Significance: CPz-FPz and FPz-C5S should no longer be standard.展开更多
目的 分析创伤性颅脑损伤血清miR-31-5p、miR-129-5p水平及其与神经功能的关系。方法 回顾性选取2022年8月—2025年8月东南大学附属徐州市中心医院收治的创伤性颅脑损伤患者169例,根据格拉斯哥昏迷量表(Glasgow coma scale,GCS)评分将...目的 分析创伤性颅脑损伤血清miR-31-5p、miR-129-5p水平及其与神经功能的关系。方法 回顾性选取2022年8月—2025年8月东南大学附属徐州市中心医院收治的创伤性颅脑损伤患者169例,根据格拉斯哥昏迷量表(Glasgow coma scale,GCS)评分将患者分为轻型组(n=64)、中型组(n=54)、重型组(n=51)。另选同期东南大学附属徐州市中心医院体检的健康者100例为对照组。比较所有受试者血清miR-31-5p、miR-129-5p水平、美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分。采用Pearson相关分析血清miR-31-5p、miR-129-5p水平与NIHSS评分的相关性,采用受试者工作特征(receiver operating characteristic,ROC)曲线分析血清miR-31-5p、miR-129-5p水平对创伤性颅脑损伤患者发生神经功能缺损的诊断价值。结果 重型组、中型组、轻型组血清miR-31-5p水平、NIHSS评分均高于对照组,血清miR-129-5p水平均低于对照组,重型组、中型组血清miR-31-5p水平、NIHSS评分均高于轻型组,血清miR-129-5p水平均低于轻型组,重型组血清miR-31-5p水平、NIHSS评分均高于中型组,血清miR-129-5p水平低于中型组(P<0.05)。血清miR-31-5p水平与NIHSS评分呈正相关(r=0.666,P<0.05),血清miR-129-5p水平与NIHSS评分呈负相关(r=-0.715,P<0.05)。ROC结果显示,血清miR-31-5p、miR-129-5p水平联合诊断创伤性颅脑损伤患者神经功能缺损的敏感度为99.3%,特异度为92.9%,AUC为0.951,预测价值较高。结论 血清miR-31-5p水平升高、miR-129-5p水平降低与创伤性颅脑损伤患者神经功能缺损程度密切相关,二者联合检测对评估神经功能缺损具有较高预测价值。展开更多
Objectives:Chemotherapy-induced lung inflammation limits the efficacy of anticancer therapies such as gefitinib in non-small cell lung cancer(NSCLC).Glutamic acid-leucine-arginine positive(ELR+)CXC chemokines and thei...Objectives:Chemotherapy-induced lung inflammation limits the efficacy of anticancer therapies such as gefitinib in non-small cell lung cancer(NSCLC).Glutamic acid-leucine-arginine positive(ELR+)CXC chemokines and their receptors,CXC chemokine receptor 1 and 2(CXCR1 and CXCR2),mediate both inflammatory responses and tumor progression.This study evaluated the effects of CXCR1/2 antagonism by G31P,a CXC motif chemokine ligand 8(CXCL8)-mutated peptide,alone or in combination with gefitinib,on lung cancer growth and chemotherapyinduced pulmonary inflammation.Methods:Human NSCLC cell lines(A549 and H460)were treated with gefitinib and/or G31P.Cell proliferation,apoptosis,and signaling pathways,including protein kinase B(AKT)and extracellular signal-regulated kinase(ERK)phosphorylation,were evaluated by cell counting kit-8(CCK-8)assay,flow cytometry,and Western blotting.An orthotopic lung tumor xenograft model was established in BALB/c nude mice to evaluate tumor growth,metastasis,cytokine expression,and lung histopathology.A bleomycin-induced lung injury model was also used to assess the anti-inflammatory effects of G31P,with or without gefitinib,by quantitative reverse transcription polymerase chain reaction(qRT-PCR)and flowcytometry of inflammatorymarkers.Results:G31P andGefitinib,either alone or combined,inhibited proliferation and migration of A549 and H460 cells in vitro.Combination treatment effectively reducedAKT and ERKphosphorylation in both cell lines.In vivo,G31Pwith gefitinib significantly suppressed tumor growth,metastasis,and increased apoptosis.G31P decreased CXCL1 and CXCL2,and tumor necrosis factoralpha(TNF-α)mRNA levels,lung hydroxyproline content,and myeloperoxidase(MPO)activity in the lungs of mice.In the bleomycin-induced lung injury model,G31P similarly reduced inflammatory responses.Conclusion:CXCR1/2 antagonismby G31P attenuates chemotherapy-induced pulmonary inflammation and enhances the anti-tumor efficacy of gefitinib in NSCLC.These findings support the therapeutic potential of G31P as an adjuvant to epidermal growth factor receptor tyrosine kinase inhibitors(EGFR-TKIs)to improve clinical outcomes by limiting inflammation.展开更多
文摘Objective: To compare the intraoperative signal-to-noise ratio (SNR), reproducibility and rapidity of popliteal fossa (PF), optimized P37, standard P37 and P31 potentials. Methods: Raw sweeps and 11 averages doubling sweep number from 2 to 2048 were compared in 37 patients undergoing scoliosis surgery. Optimized (highest amplitude or SNR) P37 derivations were Cz-CPc (22), CPz-CPc (27), Pz-CPc (7), iCPi-CPc (8), CPi-CPc (1), Cz-Pz (2)orPz-FPz (3), and in two patients with non-decussation, Cz-CPi (1) or CPz-CPi (3). Standard P37 and P31 derivations were CPz-FPz and FPz-C5S. Signal amplitude was measured in 2048 sweep averages; peak noise was measured in raw sweeps and ±averages; SNR was amplitude/noise. Visual superimposability and < 20-30%amplitude variation determined reproducibility. Sweeps to reproducibility determined rapidity. Results: The SNR order was PFoptimized P37 > standard P37 > P31. Mean optimized P37 SNR advantages over the standard P37 and P31 were 2.1:1 and 4.9:1. SNR had powerful non-linear correlations to reproducibility and rapidity. Median sweeps to reproducibility were PF: 2, optimized P37: 128, standard P37: 512 and P31: 1024. EEG noise was greatest in FPz derivations. Burst-suppression increased scalp potential SNR and rapidity. Conclusions: Optimized P37 and PF recordings are most rapidly reproducible due to superior SNRs and are recommended. FPz should be avoided. Burst-suppression may be desirable. Significance: CPz-FPz and FPz-C5S should no longer be standard.
文摘目的 分析创伤性颅脑损伤血清miR-31-5p、miR-129-5p水平及其与神经功能的关系。方法 回顾性选取2022年8月—2025年8月东南大学附属徐州市中心医院收治的创伤性颅脑损伤患者169例,根据格拉斯哥昏迷量表(Glasgow coma scale,GCS)评分将患者分为轻型组(n=64)、中型组(n=54)、重型组(n=51)。另选同期东南大学附属徐州市中心医院体检的健康者100例为对照组。比较所有受试者血清miR-31-5p、miR-129-5p水平、美国国立卫生研究院卒中量表(National Institutes of Health Stroke Scale,NIHSS)评分。采用Pearson相关分析血清miR-31-5p、miR-129-5p水平与NIHSS评分的相关性,采用受试者工作特征(receiver operating characteristic,ROC)曲线分析血清miR-31-5p、miR-129-5p水平对创伤性颅脑损伤患者发生神经功能缺损的诊断价值。结果 重型组、中型组、轻型组血清miR-31-5p水平、NIHSS评分均高于对照组,血清miR-129-5p水平均低于对照组,重型组、中型组血清miR-31-5p水平、NIHSS评分均高于轻型组,血清miR-129-5p水平均低于轻型组,重型组血清miR-31-5p水平、NIHSS评分均高于中型组,血清miR-129-5p水平低于中型组(P<0.05)。血清miR-31-5p水平与NIHSS评分呈正相关(r=0.666,P<0.05),血清miR-129-5p水平与NIHSS评分呈负相关(r=-0.715,P<0.05)。ROC结果显示,血清miR-31-5p、miR-129-5p水平联合诊断创伤性颅脑损伤患者神经功能缺损的敏感度为99.3%,特异度为92.9%,AUC为0.951,预测价值较高。结论 血清miR-31-5p水平升高、miR-129-5p水平降低与创伤性颅脑损伤患者神经功能缺损程度密切相关,二者联合检测对评估神经功能缺损具有较高预测价值。
基金supported by grants from the National Natural Science Foundation of China,No.U1604174Health Commission of Henan Province-Province and Ministry Co-Construction Project,Nos.20170123,SBGJ202002004Health Commission of Henan Province-Research Innovative Talents Project(51282).
文摘Objectives:Chemotherapy-induced lung inflammation limits the efficacy of anticancer therapies such as gefitinib in non-small cell lung cancer(NSCLC).Glutamic acid-leucine-arginine positive(ELR+)CXC chemokines and their receptors,CXC chemokine receptor 1 and 2(CXCR1 and CXCR2),mediate both inflammatory responses and tumor progression.This study evaluated the effects of CXCR1/2 antagonism by G31P,a CXC motif chemokine ligand 8(CXCL8)-mutated peptide,alone or in combination with gefitinib,on lung cancer growth and chemotherapyinduced pulmonary inflammation.Methods:Human NSCLC cell lines(A549 and H460)were treated with gefitinib and/or G31P.Cell proliferation,apoptosis,and signaling pathways,including protein kinase B(AKT)and extracellular signal-regulated kinase(ERK)phosphorylation,were evaluated by cell counting kit-8(CCK-8)assay,flow cytometry,and Western blotting.An orthotopic lung tumor xenograft model was established in BALB/c nude mice to evaluate tumor growth,metastasis,cytokine expression,and lung histopathology.A bleomycin-induced lung injury model was also used to assess the anti-inflammatory effects of G31P,with or without gefitinib,by quantitative reverse transcription polymerase chain reaction(qRT-PCR)and flowcytometry of inflammatorymarkers.Results:G31P andGefitinib,either alone or combined,inhibited proliferation and migration of A549 and H460 cells in vitro.Combination treatment effectively reducedAKT and ERKphosphorylation in both cell lines.In vivo,G31Pwith gefitinib significantly suppressed tumor growth,metastasis,and increased apoptosis.G31P decreased CXCL1 and CXCL2,and tumor necrosis factoralpha(TNF-α)mRNA levels,lung hydroxyproline content,and myeloperoxidase(MPO)activity in the lungs of mice.In the bleomycin-induced lung injury model,G31P similarly reduced inflammatory responses.Conclusion:CXCR1/2 antagonismby G31P attenuates chemotherapy-induced pulmonary inflammation and enhances the anti-tumor efficacy of gefitinib in NSCLC.These findings support the therapeutic potential of G31P as an adjuvant to epidermal growth factor receptor tyrosine kinase inhibitors(EGFR-TKIs)to improve clinical outcomes by limiting inflammation.
