P2X_(1) receptors and adrenoceptors are mainly responsible for vasoconstriction in a variety of blood vessels.However,previous studies have shown that α,β-methylene adenosine 5'-triphosphate(α,β-MeATP),a stabl...P2X_(1) receptors and adrenoceptors are mainly responsible for vasoconstriction in a variety of blood vessels.However,previous studies have shown that α,β-methylene adenosine 5'-triphosphate(α,β-MeATP),a stable analogue of ATP,can induce both pressor and depressor responses in la boratory animals.In this study,the effects of increasing intravenous doses of α,β-MeATP and noradrenaline(NA)(0-30 nmol/kg)administered at 20 min intervals on systolic(SBP),diastolic(DBP)and mean(MBP)blood pressure in groups of anesthetized mice(n=6)were compared.Both α,β-MeATP and NA caused transient,dose-dependent increases in SBP,DBP and MBP but the effect of α,β-MeATP was more rapid and significantly larger at doses of 10 and 30 nmol/kg(P<0.01).At the dose of 30 nmol/kg,α,β-MeATP increased SBP,DBP and MBP by 65.8±7.0,65.7±5.0 and 65.7±5.5 mmHg,respectively,compared to increases of 36.8±4.6,33.3±4.9 and 34.5±4.7 mmHg,respectively,produced by NA.These results indicate P2X_(1) receptors play an important role in BP regulation although purinergic vasoconstriction alone may not explain the more potent pressor response to α,β-MeATP in the anesthetized mouse.展开更多
文摘目的: 观察慢性膀胱出口梗阻后P2X1受体的表达水平.方法: 雌性Wistar大鼠70只,体质量250～300 g,40只为实验组,30只同龄雌性Wistar大鼠为对照组.在部分膀胱出口梗阻的动物模型成功建立后8 wk开始试验,采用免疫组织化学方法结合IMAGE-PRO图像处理软件对大鼠膀胱P2X1的表达(即阳性颗粒在某个视野下所占的百分比)进行定量研究.结果: 部分膀胱出口梗阻后大鼠较对照组大鼠膀胱的体积增大,黏膜层增厚,P2X1受体的表达水平平均为在膀胱平滑肌层[(9.60±4.78) vs (2.31±1.19)] (P<0.01),在膀胱黏膜层[(0.12±0.03) vs (0.06±0.02)] (P<0.01),浆膜层[(0.04±0.02) vs (0.03±0.01)] (P>0.05),尿道[(6.05±2.25) vs (1.48±0.14)] (P<0.01),慢性膀胱出口梗阻后P2X1受体的表达水平均明显高于对照组.结论: 膀胱出口部分梗阻后,P2X1受体在膀胱各个部位表达均升高.推测P2X1可能参与了慢性膀胱出口梗阻后膀胱的生理或病理功能的调节.
文摘P2X_(1) receptors and adrenoceptors are mainly responsible for vasoconstriction in a variety of blood vessels.However,previous studies have shown that α,β-methylene adenosine 5'-triphosphate(α,β-MeATP),a stable analogue of ATP,can induce both pressor and depressor responses in la boratory animals.In this study,the effects of increasing intravenous doses of α,β-MeATP and noradrenaline(NA)(0-30 nmol/kg)administered at 20 min intervals on systolic(SBP),diastolic(DBP)and mean(MBP)blood pressure in groups of anesthetized mice(n=6)were compared.Both α,β-MeATP and NA caused transient,dose-dependent increases in SBP,DBP and MBP but the effect of α,β-MeATP was more rapid and significantly larger at doses of 10 and 30 nmol/kg(P<0.01).At the dose of 30 nmol/kg,α,β-MeATP increased SBP,DBP and MBP by 65.8±7.0,65.7±5.0 and 65.7±5.5 mmHg,respectively,compared to increases of 36.8±4.6,33.3±4.9 and 34.5±4.7 mmHg,respectively,produced by NA.These results indicate P2X_(1) receptors play an important role in BP regulation although purinergic vasoconstriction alone may not explain the more potent pressor response to α,β-MeATP in the anesthetized mouse.
