Objective:To assess the in vivo antimalarial potential of various solvent extracts and fractions of Trema orientalis.Methods:In this study,the animal model of antimalarial activity was employed using Plasmodium berghe...Objective:To assess the in vivo antimalarial potential of various solvent extracts and fractions of Trema orientalis.Methods:In this study,the animal model of antimalarial activity was employed using Plasmodium berghei-induced mice.The crude methanol extract was fractionated using vacuum liquid chromatography in the order of increasing polarity using dichloromethane,ethylacetate and methanol.Percentages of parasitemia and clearance were used as indices for antiplasmodial activities.The full blood count was also assayed while the gas chromatography-mass spectrometer analysis of the most potent fraction was carried out to detect the active compounds presenting in it.Results:Dichloromethane fraction had the least percentage of parasitemia[(0.19±0.07)%]and the highest percentage of clearance[(91.74±8.38)%]at the highest dose used(200 mg/kg body weight)after day 7 relative to the artemisinin control which cleared the parasite after day 3.The ethylacetate fraction showed the least percentage of clearance[(70.52±5.64)%]at the highest dose used(200 mg/kg body weight)after day 7.Conclusions:The results obtained showed that purification enhanced the antiplasmodial activity of Trema orientalis in Plasmodium berghei-induced malaria in mice.The antiplasmodial activity of the dichloromethane is a strong indication that the fraction,if purified further,may contain drug candidates for the treatment of malaria in the nearest future.展开更多
Lemon grass(Cymbopogon citratus Stapf) is a popular alternative to western medicines for a number of conditions, including fevers,muscle soreness and superficial infections in Nigeria.In addition to its already report...Lemon grass(Cymbopogon citratus Stapf) is a popular alternative to western medicines for a number of conditions, including fevers,muscle soreness and superficial infections in Nigeria.In addition to its already reported suppressive effects against P.berghei infection,this study sought to determine its repository and blood schizonticidal activities in established P.berghei infection using Swiss albino mice as models.Mice weighing on average, between 15 and 25g were given 103mg/kg,155mg/kg and 310mg/kg/day of the crude aqueous extract of cymbopogon citratus stapf,in the 4-day test,24-hour Rane test and 72-hour Rane test.The effects of these doses of the extract were then compared with chloroquine(5mg/kg/day) and sulphadoxine/pyrimethamine (3mg/kg/day).We report an average percentage suppressive repository activity of 65.8%for the extract at a dose of 310mg/kg and a blood schizonticidal activity that increased from 68.33%in the 24-hour Rane test to 92%in the 72-hour Rane test for the same dose of extract.The crude aqueous extract of C.citratus stapf thus has significant repository and blood schizonticidal activities against established P.berghei infection in Swiss albino mice compare to that of pyrimethamine and sulphadoxine/pyrimethamine respectively.展开更多
文摘Objective:To assess the in vivo antimalarial potential of various solvent extracts and fractions of Trema orientalis.Methods:In this study,the animal model of antimalarial activity was employed using Plasmodium berghei-induced mice.The crude methanol extract was fractionated using vacuum liquid chromatography in the order of increasing polarity using dichloromethane,ethylacetate and methanol.Percentages of parasitemia and clearance were used as indices for antiplasmodial activities.The full blood count was also assayed while the gas chromatography-mass spectrometer analysis of the most potent fraction was carried out to detect the active compounds presenting in it.Results:Dichloromethane fraction had the least percentage of parasitemia[(0.19±0.07)%]and the highest percentage of clearance[(91.74±8.38)%]at the highest dose used(200 mg/kg body weight)after day 7 relative to the artemisinin control which cleared the parasite after day 3.The ethylacetate fraction showed the least percentage of clearance[(70.52±5.64)%]at the highest dose used(200 mg/kg body weight)after day 7.Conclusions:The results obtained showed that purification enhanced the antiplasmodial activity of Trema orientalis in Plasmodium berghei-induced malaria in mice.The antiplasmodial activity of the dichloromethane is a strong indication that the fraction,if purified further,may contain drug candidates for the treatment of malaria in the nearest future.
文摘Lemon grass(Cymbopogon citratus Stapf) is a popular alternative to western medicines for a number of conditions, including fevers,muscle soreness and superficial infections in Nigeria.In addition to its already reported suppressive effects against P.berghei infection,this study sought to determine its repository and blood schizonticidal activities in established P.berghei infection using Swiss albino mice as models.Mice weighing on average, between 15 and 25g were given 103mg/kg,155mg/kg and 310mg/kg/day of the crude aqueous extract of cymbopogon citratus stapf,in the 4-day test,24-hour Rane test and 72-hour Rane test.The effects of these doses of the extract were then compared with chloroquine(5mg/kg/day) and sulphadoxine/pyrimethamine (3mg/kg/day).We report an average percentage suppressive repository activity of 65.8%for the extract at a dose of 310mg/kg and a blood schizonticidal activity that increased from 68.33%in the 24-hour Rane test to 92%in the 72-hour Rane test for the same dose of extract.The crude aqueous extract of C.citratus stapf thus has significant repository and blood schizonticidal activities against established P.berghei infection in Swiss albino mice compare to that of pyrimethamine and sulphadoxine/pyrimethamine respectively.
