Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly...Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly increased.This article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of F.taipaiensis P.Y.Li to the cultivation process of F.taipaiensis P.Y.Li.The aim is to identify suitable reference strains for the artificial cultivation and industrial development of F.taipaiensis P.Y.Li by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in F.taipaiensis P.Y.Li.The experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven groups.Compared to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area index.The content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline content.The activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT activities.The malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of CK.Gene expression corresponding to these three enzymes also increased variably,with POD expression in Y2 being the highest at 2.73 times that of CK.SOD and CAT expression in Y12 were the highest,at 1.84 and 4.39 times that of CK,respectively.These results indicate that inoculating phosphate solubilizing bacteria can enhance the growth of F.taipaiensis P.Y.Li,with the mixed inoculation groups W12 and Y12 demonstrating superior effects.This lays a theoretical foundation for selecting bacterial fertilizers in the cultivation process of F.taipaiensis P.Y.Li.展开更多
目的解析百合科贝母属Fritillaria多年生药用植物太白贝母Fritillaria taipaiensis叶绿体全基因组的结构特征,探究其系统发育关系。方法采用CTAB法提取太白贝母叶片基因组DNA,基于Illumina novaseq X plus平台进行测序,使用GetOrganell...目的解析百合科贝母属Fritillaria多年生药用植物太白贝母Fritillaria taipaiensis叶绿体全基因组的结构特征,探究其系统发育关系。方法采用CTAB法提取太白贝母叶片基因组DNA,基于Illumina novaseq X plus平台进行测序,使用GetOrganelle组装叶绿体基因组,利用生物信息学方法比较中药材川贝母6种基原药用植物叶绿体基因组之间的结构特征及变异程度,并通过MEGA11.0软件以邻接(neighbor-joining,NJ)法构建系统发育树。结果太白贝母叶绿体基因组全长151700 bp,包括1个大单拷贝区(large single copy,LSC),1个小单拷贝区(small single copy,SSC)和1对反向重复区(inverted repeats,IRa和IRb),GC含量为36.97%。太白贝母叶绿体基因组共注释到131个基因,其中包含8个rRNA、38个t RNA、84个蛋白编码基因和1个假基因。密码子使用偏好分析显示,50.00%的密码子相对使用度大于1,且偏好以A/T结尾。共鉴定216个简单重复序列(simple sequence repeats,SSR),其中单核苷酸重复占比最高(52.78%),且主要由A和T构成,表明A/T碱基使用频率较高。边界比较分析表明,6种川贝母基原植物(川贝母、瓦布贝母、梭砂贝母、太白贝母、暗紫贝母和甘肃贝母)的叶绿体基因组边界结构保守。变异分析和共线性分析则揭示,6种川贝母基原植物叶绿体基因组变异程度较低,且均存在一个保守共线区块。系统发育分析中,贝母属9个物种聚为一支,其中6种川贝母基原物种再形成独立分支,贝母属与百合属互为姐妹支,与老鸦瓣属区分开来。结论成功组装了高质量的太白贝母叶绿体基因组,为其遗传结构解析与多样性研究提供了基础。展开更多
基金This work was funded by Chongqing Municipal Technology Innovation and Application Development Program(cstc2020jscx-gksb0001)Yunnan Academician(Expert)Workstation Project(202105AF150073).
文摘Fritillaria taipaiensis P.Y.Li is a widely used medicinal herb in treating pulmonary diseases.In recent years,its wild resources have become scarce,and the demand for efficient artificial cultivation has significantly increased.This article is the first to apply phosphate solubilizing bacteria isolated from the rhizosphere soil of F.taipaiensis P.Y.Li to the cultivation process of F.taipaiensis P.Y.Li.The aim is to identify suitable reference strains for the artificial cultivation and industrial development of F.taipaiensis P.Y.Li by examining the effects of various phosphate solubilizing bacteria and their combinations on photosynthesis,physiological and biochemical properties,and gene expression related to the protective enzyme system in F.taipaiensis P.Y.Li.The experiment,conducted in pots at room temperature,included a control group(CK)and groups inoculated with inorganic phosphorussolubilizing bacteria:W1(Bacillus cereus),W2(Serratia plymuthica),W12(Bacillus cereus and Serratia plymuthica),and groups inoculated with organophosphorus-solubilizing bacteria:Y1(Bacillus cereus),Y2(Bacillus cereus),Y12(Bacillus cereus and Bacillus cereus),totaling seven groups.Compared to CK,most growth indices in the bacterial addition groups showed significant differences,with W12 achieving the highest values in all indices except the leaf area index.The content of photosynthetic pigments,photosynthetic parameters,and osmoregulatory substances increased variably in each bacterial treatment group.W12 exhibited the highest content of chlorophyll a and soluble protein,while W1 had the highest free proline content.The activities of peroxidase(POD),superoxide dismutase(SOD),and catalase(CAT)in all inoculated groups were higher than in CK,with significant changes in SOD and CAT activities.The malondialdehyde(MDA)content in all inoculated groups was lower than in CK,with Y12 being the lowest,at approximately 30%of CK.Gene expression corresponding to these three enzymes also increased variably,with POD expression in Y2 being the highest at 2.73 times that of CK.SOD and CAT expression in Y12 were the highest,at 1.84 and 4.39 times that of CK,respectively.These results indicate that inoculating phosphate solubilizing bacteria can enhance the growth of F.taipaiensis P.Y.Li,with the mixed inoculation groups W12 and Y12 demonstrating superior effects.This lays a theoretical foundation for selecting bacterial fertilizers in the cultivation process of F.taipaiensis P.Y.Li.
文摘目的解析百合科贝母属Fritillaria多年生药用植物太白贝母Fritillaria taipaiensis叶绿体全基因组的结构特征,探究其系统发育关系。方法采用CTAB法提取太白贝母叶片基因组DNA,基于Illumina novaseq X plus平台进行测序,使用GetOrganelle组装叶绿体基因组,利用生物信息学方法比较中药材川贝母6种基原药用植物叶绿体基因组之间的结构特征及变异程度,并通过MEGA11.0软件以邻接(neighbor-joining,NJ)法构建系统发育树。结果太白贝母叶绿体基因组全长151700 bp,包括1个大单拷贝区(large single copy,LSC),1个小单拷贝区(small single copy,SSC)和1对反向重复区(inverted repeats,IRa和IRb),GC含量为36.97%。太白贝母叶绿体基因组共注释到131个基因,其中包含8个rRNA、38个t RNA、84个蛋白编码基因和1个假基因。密码子使用偏好分析显示,50.00%的密码子相对使用度大于1,且偏好以A/T结尾。共鉴定216个简单重复序列(simple sequence repeats,SSR),其中单核苷酸重复占比最高(52.78%),且主要由A和T构成,表明A/T碱基使用频率较高。边界比较分析表明,6种川贝母基原植物(川贝母、瓦布贝母、梭砂贝母、太白贝母、暗紫贝母和甘肃贝母)的叶绿体基因组边界结构保守。变异分析和共线性分析则揭示,6种川贝母基原植物叶绿体基因组变异程度较低,且均存在一个保守共线区块。系统发育分析中,贝母属9个物种聚为一支,其中6种川贝母基原物种再形成独立分支,贝母属与百合属互为姐妹支,与老鸦瓣属区分开来。结论成功组装了高质量的太白贝母叶绿体基因组,为其遗传结构解析与多样性研究提供了基础。
