In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and t...In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.展开更多
In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students＇ learning efficiency. In this paper, the current situation of the current ...In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students＇ learning efficiency. In this paper, the current situation of the current physical education is analyzed, and also related suggestions on the optimization of the P.E. course teaching plan are proposed.展开更多
Mercury and its organic compounds have been of severe concern worldwide due to their damage to the ecosystem and human health. The development of effective and affordable technology to monitor and signal the presence ...Mercury and its organic compounds have been of severe concern worldwide due to their damage to the ecosystem and human health. The development of effective and affordable technology to monitor and signal the presence of bioavailable mercury is an urgent need.The Mer gene is a mercury-responsive resistant gene, and a mercury-sensing recombinant luminescent bacterium using the Mer gene was constructed in this study. The mer operon from marine Pseudomonas putida strain SP1 was amplified and fused with prompterless lux CDABE in the p UCD615 plasmid within Escherichia coli cells, resulting in p THE30–E. coli.The recombinant strain showed high sensitivity and specificity. The detection limit of Hg^2＋was 5 nmol/L, and distinct luminescence could be detected in 30 min. Cd^2＋, Cu^2＋, Zn^2＋, Ca^2＋,Pb^2＋, Mg^2＋, Mn^2＋, and Al^3＋did not interfere with the detection over a range of 10-5–1 m M.Application of recombinant luminescent bacteria testing in environmental samples has been a controversial issue： especially for metal-sensing recombinant strains, false negatives caused by high cytotoxicity are one of the most important issues when applying recombinant luminescent bacteria in biomonitoring of heavy metals. In this study, by establishing an internal standard approach, the false negative problem was overcome;furthermore, the method can also help to estimate the suspected mercury concentration,which ensures high detection sensitivity of bioavailable Hg2＋.展开更多
应用学习型组织(Learn ing O rgan ization)理论,将班级、代表队、体协等作为学习型组织进行整体设计;学习型公共体育教学模式由体育必修课、体育选修课、课外体育锻炼、竞技体育训练课组成“学习平台”;以体育课堂教学为中心,建立学习...应用学习型组织(Learn ing O rgan ization)理论,将班级、代表队、体协等作为学习型组织进行整体设计;学习型公共体育教学模式由体育必修课、体育选修课、课外体育锻炼、竞技体育训练课组成“学习平台”;以体育课堂教学为中心,建立学习型体育教学的导向机制;以培养学生主动学习为突破口,建立学生主动学习的运行机制;构建课内外一体化的学习大环境,建立实践与应用机制。展开更多
文摘In this paper, the physical education information teaching design system is researched and developed based on VB6.0 optimization platform. According to the national physical education and health course standards and the fundamental theories of the constructivisrn, the author adopts the modular-design development method to design two functional areas which are the displaying of the teaching design contents and the online information database （nine modules in total） on the basis of the P.E. teaching design flow. Importantly, this system features the compatibility, scalability and practical applicability, and can effectively improve the ability of P.E. teachers to design their teaching, and hence provides a guarantee for the improvement of teaching quality and efficiency.
文摘In the new period, the physical education in colleges and universities is of greater and greater significance for the enhancement of students＇ learning efficiency. In this paper, the current situation of the current physical education is analyzed, and also related suggestions on the optimization of the P.E. course teaching plan are proposed.
基金supported by the National Natural Science Foundation of China (No. 21377065)the 863 National High-Tech Research and Development Program (No. 2014AA06A506)
文摘Mercury and its organic compounds have been of severe concern worldwide due to their damage to the ecosystem and human health. The development of effective and affordable technology to monitor and signal the presence of bioavailable mercury is an urgent need.The Mer gene is a mercury-responsive resistant gene, and a mercury-sensing recombinant luminescent bacterium using the Mer gene was constructed in this study. The mer operon from marine Pseudomonas putida strain SP1 was amplified and fused with prompterless lux CDABE in the p UCD615 plasmid within Escherichia coli cells, resulting in p THE30–E. coli.The recombinant strain showed high sensitivity and specificity. The detection limit of Hg^2＋was 5 nmol/L, and distinct luminescence could be detected in 30 min. Cd^2＋, Cu^2＋, Zn^2＋, Ca^2＋,Pb^2＋, Mg^2＋, Mn^2＋, and Al^3＋did not interfere with the detection over a range of 10-5–1 m M.Application of recombinant luminescent bacteria testing in environmental samples has been a controversial issue： especially for metal-sensing recombinant strains, false negatives caused by high cytotoxicity are one of the most important issues when applying recombinant luminescent bacteria in biomonitoring of heavy metals. In this study, by establishing an internal standard approach, the false negative problem was overcome;furthermore, the method can also help to estimate the suspected mercury concentration,which ensures high detection sensitivity of bioavailable Hg2＋.
文摘应用学习型组织(Learn ing O rgan ization)理论,将班级、代表队、体协等作为学习型组织进行整体设计;学习型公共体育教学模式由体育必修课、体育选修课、课外体育锻炼、竞技体育训练课组成“学习平台”;以体育课堂教学为中心,建立学习型体育教学的导向机制;以培养学生主动学习为突破口,建立学生主动学习的运行机制;构建课内外一体化的学习大环境,建立实践与应用机制。
