The research question being studied in this paper is how do different types of bacteria as food (Pseudomonas fluorescens and Bacillus megaterium) affect the lifespan of Caenorhabditis elegans in dpy-11 mutant-type and...The research question being studied in this paper is how do different types of bacteria as food (Pseudomonas fluorescens and Bacillus megaterium) affect the lifespan of Caenorhabditis elegans in dpy-11 mutant-type and wild-type? P. fluorescens and B. megaterium will be the two pathogens that will be tested on two different types of C. elegans: mutant-type dpy-11 and wild-type. From the analysis of primary articles studying these pathogens, it can be concluded that P. fluorescens and B. megaterium are decent contenders for allowing C. elegans to grow and possibly extend the lifespan of it. P. fluorescens will allow the lifespan of the two types of nematodes to be longer. Additionally, the mu-tant-type dpy-11 of C. elegans will have a much longer lifespan, even double, compared to that of the wild-type. The results showed P. fluorescens had a longer lifespan than B. megaterium but not as long as C. elegans’ main food source, E. coli. C. elegans mutant dpy-11 had a longer lifespan than the wild-type. Furthermore, there were no C. elegans present in the B. megaterium wild-type plates.展开更多
The isolation of bacteria from the rhizosphere soil of different plants and locations in Diwaniyah Governorate and their diagnosis by two methods.Isolation and routine molecular diagnosis revealed ten bacterial isolat...The isolation of bacteria from the rhizosphere soil of different plants and locations in Diwaniyah Governorate and their diagnosis by two methods.Isolation and routine molecular diagnosis revealed ten bacterial isolates with the attributes of P.fluorescens out of fifteen local isolates that are represented by the following codes and sequences(P.f9,P.f8,P.f6,P.f5,P.f4,P.f2,P.f1,P.f14,P.f13,P.f11).Results also confirmed the diagnosis of bacterial isolates by biochemical and molecular tests using a specialized primer to amplify the bp698 region of the 16S ribosomal RNA gene,approved by Macrogen/Korea.The test efficiency in dissolving solid phosphate by P.fluorescens bacteria showed that the most effective is the(P.f1)isolate,giving the highest score effectiveness in mineral phosphate dissolution by the diameter of the clear zone around the colony,which was effective in phosphate dissolution up to 6.95 mm.The efficiency of the Nitrogen Fixation Test showed that the isolate(P.f5)scored the highest nitrogen-fixing efficiency amount with a value of 6.81 mg L^(-1).The quantitative amount of the hormone for each of Auxins,Cytokinins,and Gibberellins was assayed;the results with isolate(P.f1)for IAA(Auxins)gave a concentration up to 28.6μg ml^(-1),which was the most,while the production of GA3 by isolate(P.f1)gave the maximum value of 36.7μg ml^(-1),and for synthesis of the hormone of Cytokinins represented by isolate(P.f2),the highest value in the production of Cytokinins hormone was recorded at 26.3μg ml^(-1).展开更多
文摘The research question being studied in this paper is how do different types of bacteria as food (Pseudomonas fluorescens and Bacillus megaterium) affect the lifespan of Caenorhabditis elegans in dpy-11 mutant-type and wild-type? P. fluorescens and B. megaterium will be the two pathogens that will be tested on two different types of C. elegans: mutant-type dpy-11 and wild-type. From the analysis of primary articles studying these pathogens, it can be concluded that P. fluorescens and B. megaterium are decent contenders for allowing C. elegans to grow and possibly extend the lifespan of it. P. fluorescens will allow the lifespan of the two types of nematodes to be longer. Additionally, the mu-tant-type dpy-11 of C. elegans will have a much longer lifespan, even double, compared to that of the wild-type. The results showed P. fluorescens had a longer lifespan than B. megaterium but not as long as C. elegans’ main food source, E. coli. C. elegans mutant dpy-11 had a longer lifespan than the wild-type. Furthermore, there were no C. elegans present in the B. megaterium wild-type plates.
文摘The isolation of bacteria from the rhizosphere soil of different plants and locations in Diwaniyah Governorate and their diagnosis by two methods.Isolation and routine molecular diagnosis revealed ten bacterial isolates with the attributes of P.fluorescens out of fifteen local isolates that are represented by the following codes and sequences(P.f9,P.f8,P.f6,P.f5,P.f4,P.f2,P.f1,P.f14,P.f13,P.f11).Results also confirmed the diagnosis of bacterial isolates by biochemical and molecular tests using a specialized primer to amplify the bp698 region of the 16S ribosomal RNA gene,approved by Macrogen/Korea.The test efficiency in dissolving solid phosphate by P.fluorescens bacteria showed that the most effective is the(P.f1)isolate,giving the highest score effectiveness in mineral phosphate dissolution by the diameter of the clear zone around the colony,which was effective in phosphate dissolution up to 6.95 mm.The efficiency of the Nitrogen Fixation Test showed that the isolate(P.f5)scored the highest nitrogen-fixing efficiency amount with a value of 6.81 mg L^(-1).The quantitative amount of the hormone for each of Auxins,Cytokinins,and Gibberellins was assayed;the results with isolate(P.f1)for IAA(Auxins)gave a concentration up to 28.6μg ml^(-1),which was the most,while the production of GA3 by isolate(P.f1)gave the maximum value of 36.7μg ml^(-1),and for synthesis of the hormone of Cytokinins represented by isolate(P.f2),the highest value in the production of Cytokinins hormone was recorded at 26.3μg ml^(-1).
