Translocation of absorbed phosphorus （P） from metabolically inactive sites to active sites in plants growing under P deprivation may increase its P utilization efficiency （PUE）. Acclimation to phosphate （Pi） sta...Translocation of absorbed phosphorus （P） from metabolically inactive sites to active sites in plants growing under P deprivation may increase its P utilization efficiency （PUE）. Acclimation to phosphate （Pi） starvation may be caused by a differential storage pool of vacuolar P, its release, and the intensity of re-translocation of absorbed P as P starvation inducible environmental cues （PSlEC） from ambient environment. Biomass assay and three P forms, namely inorganic （Pi）, organic （Po）, and acid-soluble total （Ptas） were estimated in Brassica cultivars exposed to 10 d P deprivation in the culture media. Considering that -aPi/at denotes the rate of Pi release, Pi release velocity （RSPi） was determined as the tangent to the equations obtained for Pi f（t） at the mean point in the period of greatest Pi decrease, whereas the inverse of the RSPi was an estimate of the internal Pi buffering capacity （IBCPi）. Inter cultivar variations in size of the non-metabolic Pi pool, RSPi, re-translocation of Pi from less to more active metabolic sites, and preferential Pi source and sink compartments were evaluated under P starvation. The cultivar ＇Brown Raya＇ showed the highest Pi storage ability under adequate external P supply, and a more intensive release than ＇Rain Bow＇ and ＇Dunkled＇ under P stress. Cultivar ＇B.S.A＇ was inferior to ＇Con-l＇ in its ability to store and use Pi. Roots and upper leaves were the main sink of Pi stored in the lower and middle leaves of all cultivars and showed lower IBCPi and larger RSPi values than lower and middle leaves. In another trial, six cultivars were exposed to P-free nutrition for 29 d after initial feeding on optimum nutrition for 15 d. With variable magnitude, all of the cultivars re-translocatad P from the above ground parts to their roots under P starvation, and [P] at 44 d after transplanting was higher in developing leaves compared with developed leaves. Under P deprivation, translocation of absorbed P from metabolically inactive to active sites may have helped the tolerant cultivars to establish a better rooting system, which provided a basis for tolerance against P starvation and increased PUE. A better understanding of the extent to which changes in the flux of P absorption and re-translocation under PSIEC will help to scavenge Pi from bound P reserves and will bring more sparingly soluble P into cropping systems and obtain capitalization of P reserves.展开更多
A single-frequency pulsed holmium-doped yttrium lithium fluoride(Ho:YLF)amplifier pumped by a Tm-doped fiber laser was demonstrated.The seed was an injection-seeded Q-switched Ho:YLF laser.The output energy from the s...A single-frequency pulsed holmium-doped yttrium lithium fluoride(Ho:YLF)amplifier pumped by a Tm-doped fiber laser was demonstrated.The seed was an injection-seeded Q-switched Ho:YLF laser.The output energy from the singlefrequency pulsed amplifier was 24.2 mJ,with a pulse width of 250 ns at a pulse repetition frequency(PRF)of 100 Hz.The energy stability during 30 min was improved to 1%after the single-frequency pulsed Ho:YLF laser was amplified.The line width of the single-frequency pulsed spectrum of the Ho:YLF amplifier was 2.81 MHz.The single-frequency pulsed Ho:YLF amplifier can be applied to differential absorption lidar(DIAL),since its output spectrum is around the P12 CO2 absorption line.展开更多
基金the Ministry of Education, Science, Sports and Culture (MEXT),Japan.
文摘Translocation of absorbed phosphorus （P） from metabolically inactive sites to active sites in plants growing under P deprivation may increase its P utilization efficiency （PUE）. Acclimation to phosphate （Pi） starvation may be caused by a differential storage pool of vacuolar P, its release, and the intensity of re-translocation of absorbed P as P starvation inducible environmental cues （PSlEC） from ambient environment. Biomass assay and three P forms, namely inorganic （Pi）, organic （Po）, and acid-soluble total （Ptas） were estimated in Brassica cultivars exposed to 10 d P deprivation in the culture media. Considering that -aPi/at denotes the rate of Pi release, Pi release velocity （RSPi） was determined as the tangent to the equations obtained for Pi f（t） at the mean point in the period of greatest Pi decrease, whereas the inverse of the RSPi was an estimate of the internal Pi buffering capacity （IBCPi）. Inter cultivar variations in size of the non-metabolic Pi pool, RSPi, re-translocation of Pi from less to more active metabolic sites, and preferential Pi source and sink compartments were evaluated under P starvation. The cultivar ＇Brown Raya＇ showed the highest Pi storage ability under adequate external P supply, and a more intensive release than ＇Rain Bow＇ and ＇Dunkled＇ under P stress. Cultivar ＇B.S.A＇ was inferior to ＇Con-l＇ in its ability to store and use Pi. Roots and upper leaves were the main sink of Pi stored in the lower and middle leaves of all cultivars and showed lower IBCPi and larger RSPi values than lower and middle leaves. In another trial, six cultivars were exposed to P-free nutrition for 29 d after initial feeding on optimum nutrition for 15 d. With variable magnitude, all of the cultivars re-translocatad P from the above ground parts to their roots under P starvation, and [P] at 44 d after transplanting was higher in developing leaves compared with developed leaves. Under P deprivation, translocation of absorbed P from metabolically inactive to active sites may have helped the tolerant cultivars to establish a better rooting system, which provided a basis for tolerance against P starvation and increased PUE. A better understanding of the extent to which changes in the flux of P absorption and re-translocation under PSIEC will help to scavenge Pi from bound P reserves and will bring more sparingly soluble P into cropping systems and obtain capitalization of P reserves.
基金supported by the National Natural Science Foundation of China(No.51572053)。
文摘A single-frequency pulsed holmium-doped yttrium lithium fluoride(Ho:YLF)amplifier pumped by a Tm-doped fiber laser was demonstrated.The seed was an injection-seeded Q-switched Ho:YLF laser.The output energy from the singlefrequency pulsed amplifier was 24.2 mJ,with a pulse width of 250 ns at a pulse repetition frequency(PRF)of 100 Hz.The energy stability during 30 min was improved to 1%after the single-frequency pulsed Ho:YLF laser was amplified.The line width of the single-frequency pulsed spectrum of the Ho:YLF amplifier was 2.81 MHz.The single-frequency pulsed Ho:YLF amplifier can be applied to differential absorption lidar(DIAL),since its output spectrum is around the P12 CO2 absorption line.
