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云南库蠓源Oya病毒的分离与鉴定
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作者 李苏胜 何于雯 +3 位作者 杨振兴 孟锦昕 李楠 王静林 《中国热带医学》 北大核心 2025年第8期951-957,共7页
目的调查和监测云南省库蠓中携带的新发或未被重视的虫媒病毒,为人和动物健康保障提供依据。方法于2021年7月在云南勐腊某猪舍采用紫外诱捕灯采集库蠓,低温环境中带回实验室。约200只库蠓分装到1个离心管,研磨后取上清接种到C6/36细胞,... 目的调查和监测云南省库蠓中携带的新发或未被重视的虫媒病毒,为人和动物健康保障提供依据。方法于2021年7月在云南勐腊某猪舍采用紫外诱捕灯采集库蠓,低温环境中带回实验室。约200只库蠓分装到1个离心管,研磨后取上清接种到C6/36细胞,连续盲传数代;出现细胞病变效应(cytopathic effect,CPE)细胞,通过RNAiso Plus提取总RNA,用1%琼脂糖凝胶进行电泳;根据电泳带型,选用病毒特异引物,通过qRT-PCR检测鉴定阳性分离物种类。测定病毒种类后,设计引物扩增病毒的基因组,测序所得序列用Lasergene SeqMan拼接,通过NCBI的开放阅读框(open reading frame,ORF)预测工具分析编码区,从GenBank下载基因序列并通过MEGA 6.0构建系统发育树,分析遗传进化关系。结果1株阳性分离物(ML38-1)含Oya病毒(Oya virus,OYAV);拼接得到的基因组序列含3个片段,S片段长986 nt,编码233 aa;M片段长4481 nt,编码1433 aa;L片段长6887 nt,编码2261 aa。毒株ML38-1与2013年云南师宗采集库蠓中分离的OYAV(SZC50)同源性最高,S、M、L片段的相似性为99.8%~99.9%。系统发育树上,ML38-1的S片段与云南师宗库蠓、中国蚊子和越南库蚊中分离的OYAV(序列号:OP672242.1、JX983192.1、MH484317.1)、越南库蚊中分离的Cat Que virus(序列号:NC 024075.1)处于同一进化分支;M片段与云南师宗库蠓和中国蚊子中分离的OYAV(序列号:OP672243.1、JX983193.1)处于同一进化分支;L片段与中国分离的OYAV(序列号:P672244.1、JX983194.1、PQ463773.1、MK609491.1)和Cat Que病毒(序列号:OP129710.1)处于同一进化分支。结论从2021年在云南勐腊猪舍采集的库蠓中分离到1株OYAV,该毒株与云南师宗库蠓中分离的OYAV高度同源。OYAV在云南省的库蠓中存在扩散,应持续加强监测。 展开更多
关键词 库蠓 oya病毒 分离与鉴定 基因序列分析
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广东省阿卡斑病毒和OYAV的检测及分子特征研究 被引量:10
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作者 吴德 张欣 +5 位作者 张欢 谈琦琪 周惠琼 王环宇 梁国栋 宋铁 《病毒学报》 CAS CSCD 北大核心 2020年第1期84-91,共8页
阿卡斑病毒(Akabane virus,AKV)和Oya病毒(Oya virus,OYAV)均属于泛布尼亚病毒科、正布尼亚病毒属的两种病毒,可引起动物疾病,目前广东省尚不清楚是否存在这两种病毒。为了解这两种病毒在广东省的存在状况及分子特征,2018年4月至7月,分... 阿卡斑病毒(Akabane virus,AKV)和Oya病毒(Oya virus,OYAV)均属于泛布尼亚病毒科、正布尼亚病毒属的两种病毒,可引起动物疾病,目前广东省尚不清楚是否存在这两种病毒。为了解这两种病毒在广东省的存在状况及分子特征,2018年4月至7月,分别在广东省的佛山、信宜、河源和阳江四个地市利用人诱法和诱蚊灯法采集吸血昆虫标本。采集的标本分类后,按20~50只/批,蠓虫按200~500只/批分装,液氮保存运输。标本破碎离心后,上清用于接种细胞和病毒核酸提取。Real-time RT-PCR检测AKV和OYAV阳性的样本用于S基因的扩增、测序和系统进化分析。本次研究共采集各类吸血昆虫421批次,Real-time RT-PCR检测AKV阳性10份,均来自蠓虫标本,RT-PCR检测AKV阳性5份,其中4份成功测序;Real-time RT-PCR检测OYAV阳性15份,其中蠓虫13份、中华按蚊和三带喙库蚊各1份,RT-PCR检测OYAV阳性6份,其中4份成功测序。病毒分离结果均为阴性。进化分析结果显示,所有序列共分为5个血清群,本次研究的8条序列均位于Simbu群,其中4个新毒株与OYAV代表株NIV86209的核苷酸同源性最高可达96.87%,氨基酸同源性最高为100%;另外4株新病毒与AKV代表株GXLCH02的核苷酸同源性最高达98.60%,氨基酸同源性最高为99.57%。本研究结果揭示,AKV和OYAV在广东省的虫媒中分布广泛,主要以蠓虫分布为主,来自蠓虫中的新OYAV和AKV与其它媒介或宿主中的AKV和OYAV高度同源,未显示明显媒介差异的分子特征。 展开更多
关键词 阿卡斑病毒(AKV) oya病毒(oyaV) 正布尼亚病毒属 分子特征
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Case Study on Identification of Flood Hazard in the Lower Catchment Area of the Attanagalu Oya River Basin 被引量:1
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作者 Kithulgasmulle Lekamlage Nadeeka Chathurani Hatanpola Acharilage Sampath Arunashantha +2 位作者 Bamunu Arachchilage Sumanajith Kumara Pattinaya Marakkala Udara Idunil Thilakarathna Gargi Arachchilage Anudi Nishika Kaushalya 《Journal of Geoscience and Environment Protection》 2022年第7期305-318,共14页
The present work aims to identify flood hazards and risks, particularly to the Attanagalu Oya river basin in Gampaha district, the western province in Sri Lanka. Attanagalu Oya river catchment area periodically faced ... The present work aims to identify flood hazards and risks, particularly to the Attanagalu Oya river basin in Gampaha district, the western province in Sri Lanka. Attanagalu Oya river catchment area periodically faced flood hazards. The flood is categorized by complex like 2008, 2010, 2016, 2017, and it chose 2016 as the primary flood event. Study areas have been selected depending on data availability. Attanagalu Oya river basin is mainly focused as a study area. However, here selected only four Grama Niladhari Divisions are as a sample area. Those are;Kirindivita, Ambanvita, Thammita West, Gonagaha1. Furthermore, many flood hazards can be identified when considering the flood events history. But here selected only two years were 2010 and 2016. These two years were selected with high flood events. For the study, that flood series used 1 feet elevation contours used to identify flood levels and used LiDAR image to identify risk areas in the study site. Due to the blockage of the main waterways that discharge water into the Negombo Lagoon, limited water transportation, low lying land reclamation for development, mainly affect paddy lands and roads, and flood as a major problem identified temporarily and spatially. Eventually, the study could identify flood-prone areas and map the risk zones within the study area. 展开更多
关键词 Flood Hazards GIS and RS Attanagalu oya Risk Levels
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Sedimentological Characterization of Alluvial Gold Deposits of Betrare-Oya and Its Surroundings (Cameroon Eastern Region) 被引量:1
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作者 Daniel Mackaire Eloung Nna Paul Desiré Ndjigui +2 位作者 Joseph Quentin Yene Atangana Alexis Jacob Nyangono Abolo Constantin Maurice Ndongue 《International Journal of Geosciences》 2020年第12期783-799,共17页
Sedimentological methods such as granulometry, morphoscopy and heavy minerals analysis have been carried out to characterize alluvial gold deposits of Betare-Oya and its surroundings, with the aim to determine their o... Sedimentological methods such as granulometry, morphoscopy and heavy minerals analysis have been carried out to characterize alluvial gold deposits of Betare-Oya and its surroundings, with the aim to determine their origin and conditions in which they are formed and also to determine the mineralogical content of these deposits, their nature and distribution. It came out from this study the following: The alluvial deposits studied are sand, constituting of coarse fraction (grains), medium grains in abundant and fine grains. The average values of sorting index (S0) and coefficient of asymmetry (A) are respectively 2.53 and 0.73. These values indicate that the sand is poorly arranged and poorly sorted with a better classification or arrangement in the coarse fraction (grains). These are sediments that are deposited together by high competent currents. It would therefore probably be torrential deposits and or streams from short transport. Unused grains are dominating (80.5%) which translate a proximal source of the sediments. So it would be probably from the dismantling of the surrounding landforms. The heavy minerals studied revealed the presence of the following minerals;zircon, gold, sphene, green hornblende, tourmaline, rutile, augite, hypersthene, sillimanite, glau-cophane, biotite, staurotide, and the opaque. These minerals belong to the cortege of plutonic and metamorphic rocks from a single proximal distributing province. 展开更多
关键词 Betare-oya and Its Surroundings Alluvial Gold Deposits GRANULOMETRY Morphoscopy Heavy Minerals Polished Sections
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Characterization of Alluvial Gold Bearing Sediments of Betare Oya District-East Cameroon, Implication for Gold Exploration and Recovery
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作者 Germain Marie Monespérance Mboudou Kennedy Folepai Fozao +1 位作者 Olivier Annoh Njoh Christopher Mbaringong Agyingi 《Open Journal of Geology》 2017年第11期1724-1738,共15页
Field studies (location Au pits, sampling of different layers of sedimentary sequences filling the pits, panning of sediments), litho-stratigraphic investigations, grain size analysis and outlining of depositional con... Field studies (location Au pits, sampling of different layers of sedimentary sequences filling the pits, panning of sediments), litho-stratigraphic investigations, grain size analysis and outlining of depositional conditions revealed a characteristic fining upward sequence in all the sections of 08 Au-pits within the Betare Oya basin. A typical profile is 2.5 to 4.5 m thick and is composed of a 30 to 50 cm organic topsoil layer, underlain by a 1.5 m thick reddish brownish gold free saprolite, which is underlain by 1.5 m sandy section. From litho-stratigraphic investigations and grain size analysis results a quartz dominated gravelly 1.4 m thick layer consisting in boulders (275 mm), cobbles (2-&minus;0.0148x2 + 1.2187x + 2.0344, R2 = 0.6929 (sandy layer);2) y = 0.0617x2 + 1.0849x + 0.8097, R2 = 0.8694 (gravelly layer);and y > R2 is satisfied in both cases. This implies that Au recovery from these layers could be effective through gravity separation using sieves mounted on classifiers of 7 cm (sandy layer) and 10 cm (gravelly layer) diameter. 展开更多
关键词 Betare oya ASPECT Ratios Au Grains Yield ALLUVIAL SEDIMENTS PULL-APART Basin
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Assessment of Surface Water Quality of Bétaré-Oya Gold Mining Area (East-Cameroon)
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作者 Felaniaina Rakotondrabe Jules Remy Ndam Ngoupayou +5 位作者 Zakari Mfonka Eddy Harilala Rasolomanana Alexis Jacob Nyangono Abolo Banakeng Lucian Asone Andrew Ako Ako Miora Harivony Rakotondrabe 《Journal of Water Resource and Protection》 2017年第8期960-984,共25页
To assess the water quality in the locality of Bétaré-Oya affected by an intensive artisanal and semi mechanized mining activities, 71 samples were collected from sixteen points during the 2015-2016 hydrolog... To assess the water quality in the locality of Bétaré-Oya affected by an intensive artisanal and semi mechanized mining activities, 71 samples were collected from sixteen points during the 2015-2016 hydrological year. These points include: three in Lom river which is the main stream of the study area, five in Mari river which is one of the left bank tributaries of the Lom, in Bétaré-Oya and eight in the left and the right bank of Mari. Different physicochemical parameters such as pH, electrical conductivity (EC), alkalinity, turbidity, total suspended solids (TSS), cyanide (CN-), major elements and heavy metals (Pb, Zn, Cd, Fe, Cu, As, Mn and Cr) were analyzed. Water Quality Indices (WQI), Heavy metal Pollution Index (HPI), sodium adsorption ratio (SAR) and percent sodium (Na%) were also computed to evaluate the suitability of water for drinking and irrigation. The results showed that the surface water from Bétaré-Oya was acidic to basic (5.40 < pH < 8.84), weakly mineralized (11.60 < EC < 122.10 μS/cm) with a high concentration of TSS (2 < TSS < 8996.00 mg/L) and turbidity (1.22 to 4758.00 NTU). The WQI scores show excellently to unsuitable quality in almost all the sampling sites. The water quality is found to be most deteriorated in Lom river and in the downstream of Mari river where an extensive mining activity is carried out, with the high WQI value of 5137.40. Based on heavy metal pollution index, the mean value was 1195.36 and thus under the critical pollution index. We thus notice a serious physical degradation by organic and mineral suspended particles as well as chemical degradation by heavy metals. This results from mining activities in the Lom river and its main tributaries such as the Mari river in the upstream part of the Sanaga basin. According to the percentage of sodium and SAR, these waters can be used for irrigation purposes in almost all types of soils. 展开更多
关键词 Bétare-oya in East Cameroon HYDROCHEMISTRY Gold Mining Water Quality INDEX HEAVY Metal Pollution INDEX
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Physicochemical Characterisation of Soils at the Gold Exploitation Sites of Bétaré-Oya District in Cameroon and Pollution Evaluation
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作者 Nchare Mominou Yaya Al Issah +1 位作者 Bahodock Sarki Elvis Kah 《Open Journal of Inorganic Chemistry》 2018年第3期81-90,共10页
Physicochemical characterization and pollution evaluation were performed on six gold mining sites at the locality of Bétaré-Oya in Cameroon. Twelve samples of twenty two sampled show contamination with arsen... Physicochemical characterization and pollution evaluation were performed on six gold mining sites at the locality of Bétaré-Oya in Cameroon. Twelve samples of twenty two sampled show contamination with arsenic, nickel and lead. Granulometric analysis performed on all samples show a predominance of coarse particle (>250 μm) and PH varying from 5.4 to 7. The pollution index and pollution load index show that primary gold exploitation site of Mbal, Nakayo and Kpawara are contaminated. The highest pollution index is recorded on releases from the primary gold mining sites of Mbal, Kpawara and Nyondéré, which means that one must have a watchful eye to the discharges produced by primary gold mining. Sorting the samples in descending order of pollution index yields: Mb3 > Kp1 > Kp2 > Mb2 > Mb1 > Mb4 > Kp3 > Na5;meaning that special emphasis should be placed on the monitoring of wash sludge such as those from the Mbal site (Mb3), where pollution index is equal to 36.29, as well as washed and waste rock. 展开更多
关键词 Bétaré-oya Goldmining POLLUTION Index CONTAMINATION
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Application of Electrical Resistivity and Chargeability Data on a GIS Platform in Delineating Auriferous Structures in a Deeply Weathered Lateritic Terrain, Eastern Cameroon 被引量:7
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作者 Albert Nih Fon Vivian Bih Che Cheo Emmanuel Suh 《International Journal of Geosciences》 2012年第5期960-971,共12页
Exploration for primary gold in tropical settings is often problematic because of deep weathering and the development of a thick soil cover. In this paper we present the results of both chargeability and resistivity s... Exploration for primary gold in tropical settings is often problematic because of deep weathering and the development of a thick soil cover. In this paper we present the results of both chargeability and resistivity surveys carried out over the Belikombone hill gold prospect (14?00' - 14?25'E, 5?25' - 6?00'N) in the Betare Oya area (eastern Cameroon), where previous soil sampling had identified gold anomalies. The geophysical data were obtained using Syscal Junior 48 resistivity meter and the Schlumberger configuration array for both the vertical electrical soundings (VES) and horizontal profiling. These data were further built into a GIS framework and the continuity of favourable gold-bearing structures at depth modeled using WINSEV, RED2INV and SURFER extensions softwares. IP (Induced Polarization)-chargeability and resistivity data combined, have identified irregular anomalous zones trending NE-SW. This trend is consistent with the attitude of most auriferous quartz veins exposed in artisanal pits and parallel to the regional shear zone system and foliations. The high resistivity anomalies correspond to quartz veins while the relatively high IP anomalies correspond to low sulphide ± gold concentrations in the quartz veins. Modeling IP-chargeability and resistivity data prepared as contours and 3D maps, culminated to the development of an inferred, irregular and discontinuous mineralized body at depths of up to 95 m. The size and shape of this mineralized body can only later be tested by drilling to ascertain the resource. 展开更多
关键词 Gold Exploration TROPICAL SETTINGS Deep WEATHERING IP-Chargeability and RESISTIVITY Betare oya 3D Maps Cameroon
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