This experiment studied hepatic lipogenesis associated with biochemical changes in overfed Landaise Geese and China Xupu geese. Twenty healthy male Landaise geese and 20 healthy male Xupu geese, hatched on the same da...This experiment studied hepatic lipogenesis associated with biochemical changes in overfed Landaise Geese and China Xupu geese. Twenty healthy male Landaise geese and 20 healthy male Xupu geese, hatched on the same day under the same feeding conditions, were selected as experimental animals. The animals were divided into two groups and each breed served as an experimental group. Per goose of per experimental group served for a repeat. Brown rice was selected as test diet. After overfeeding for 21 d and then slaughtering, the biochemical changes of hepatic lipogenesis in the genetic susceptibility to fatty liver were evaluated. These results showed that （1） the weight of fatty liver of the two breeds of geese were 801 and 375 g （P〈0.05）, respectively. There were no differences on the abdominal fat pat, filet total and filet pectoralis major in the two breeds experimental of the geese group （P〈0.05） and no difference on body and filet skin plus subcutaneous adipose tissue （P〉0.05） was found; （2） in these two breeds of geese, there were no differences on very-lowdensity lipoprotein （VLDL）, cholesteryl esters （CE） （P〈0.05）, free cholesterol （FC）, triglycerides （TG）, phospholipids （PL） and protein （P 〈 0.05）; （3） there were no differences on activities of malic enzyme （ME）, glucose-6-phosphatedehydrogenase （G6PDH）, acetyl-CoA-carboxylas （ACX）, fatty acid synthase （FAS）, and mRNA level of ME in the two experimental breeds of geese groups （P 〈0.05）; （4） test in Landaise geese group showed that there was no significant correlation with the specific enzymatic activities, while in Xupu geese group, the liver weight was negatively correlated to the specific activity of ACX and positively to that of ME; （5） in these overfed geese, ME activity appeared to be a major factor involved in the genetic susceptibility to hepatic steatosis and it determined the hepatic lipogenesis capacity.展开更多
The effect of brown rice and corn-based diets on fatty liver weight, carcass traits, lipids accumulation and liver composition of Landes geese (Anser anser) after feeding the same amount of each diet was investigated....The effect of brown rice and corn-based diets on fatty liver weight, carcass traits, lipids accumulation and liver composition of Landes geese (Anser anser) after feeding the same amount of each diet was investigated. After 2 weeks overfeeding, besides body weight and body weight gain increased significantly, the fatty liver weights were increased dramatically from 94.67 to 554.67 and 754.00 g in corn treatment and rice treatment, respectively, and the accumulation lipids in adipose tissues reached up to or close to 2 folds of initial weight in both treatments. Liver weight was greater in brown rice group than in corn group (P<0.05). Compared to before overfeeding, triacylglycerols (TG) in geese fatty liver increased dramatically and predominated (from 9.08% to close to or more than 90% of hepatic lipids), whereas phospholipids (PL) and cholesteryl esters (CE) decreased sharply in both treatments (P<0.05). However, the percentages of lipids, TG and PL in fatty liver were higher in brown rice treatment than in corn treatment, and that of CE was lower (P<0.05). It indicates that overfeeding chiefly results in lipids accumulation in goose fatty liver and adipose tissues. Brown rice-based diet induces greater fatty liver, and brown rice is one of high quality overfeeding ingredients instead of corn.展开更多
Obesity, one of the most common health problems, is associated with glucose and lipid metabolism abnormalities, increased cardiovascular risk and oxidative stressI1}. Aging is also associated with an increase in oxida...Obesity, one of the most common health problems, is associated with glucose and lipid metabolism abnormalities, increased cardiovascular risk and oxidative stressI1}. Aging is also associated with an increase in oxidative stress[2]. Considering that older adults are already at risk for oxidant stress, the additional metabolic perturbations of obesity aggravated the susceptibility to oxidant stress. Modulating age-related oxidative stress via dietary interventions could reduce free radical production and significantly improve health in aging.展开更多
This study explored the impact of astaxanthin(AST)supplementation on growth performance,serum lipid profile,gut morphology,and antioxidant and immune function in the intestinal mucosa of Pekin ducks subjected to overf...This study explored the impact of astaxanthin(AST)supplementation on growth performance,serum lipid profile,gut morphology,and antioxidant and immune function in the intestinal mucosa of Pekin ducks subjected to overfeeding.A total of 150 male Pekin ducks at one day of age were randomly allotted into five treatment groups with five replicates of six ducks each.The control group and ad libitum group(ALG)received a basal diet while others received basal diets supplemented with AST at 40 mg/kg(Lowdose group[LDG]),80 mg/kg(medium-dose group[MDG]),and 120 mg/kg(high-dose group[HDG]).After 1 to 14 d on basal diets(brooding phase),the ducks were assigned to the dietary treatment groups for 15 to 38 d(Grower phase)and 39 to 42 d(overfeeding period).Results indicated that AST supplementation improved final body weight and weight gain at both the grower and overfeeding phases(P<0.05).Overfeeding increased the serum lipid level,altered intestinal morphology,and led to higher expression of pro-inflammatory factors and oxidative stress biomarkers while reducing antioxidant enzyme activity,associated gene expression,and anti-inflammatory factors in the duodenal and jejunal mucosa(P<0.05).Additionally,overfeeding caused increased apoptotic cell counts in the duodenal and jejunal mucosa of the control group(P<0.05),culminating in intestinal tissue damage and dysfunction.Dietary supplementation of AST mitigated these adverse effects,alleviated intestinal damage and promoted gut health.It exerted a hypolipidemic effect,improved villi morphometrics in the duodenum,jejunum,and ileum,and enhanced the levels of interleukin-4(IL-4),soluble tumor necrosis factor-alpha receptor(sTNFaR),and transforming growth factor-beta(TGF-b)(P<0.05).It also increased the activities of antioxidant enzymes and the mRNA expression of key antioxidant-related genes,including nuclear factor erythroid 2-related factor 2(Nrf2),glutathione S-transferases(GSTs),and glutamate-cysteine ligase catalytic subunit(GCLC)(P<0.05).Moreover,it reduced the expression of pro-inflammatory factors,oxidative stress biomarkers such as reactive oxygen species(ROS)and malondialdehyde(MDA),and the number of apoptotic cells in the duodenal and jejunal mucosa(P<0.05).Immunoglobulin secretion and mucosal immunity were also significantly improved with AST supplementation(P<0.05).Variations among the AST dietary groups suggest that a medium dosage of 80 mg/kg could effectively mitigate intestinal damage from overfeeding while enhancing growth performance,antioxidant defences,and immune responses.Our results would provide a theoretical reference for using AST as a nutritional strategy to enhance gut health in ducks exposed to overfeeding.展开更多
文摘This experiment studied hepatic lipogenesis associated with biochemical changes in overfed Landaise Geese and China Xupu geese. Twenty healthy male Landaise geese and 20 healthy male Xupu geese, hatched on the same day under the same feeding conditions, were selected as experimental animals. The animals were divided into two groups and each breed served as an experimental group. Per goose of per experimental group served for a repeat. Brown rice was selected as test diet. After overfeeding for 21 d and then slaughtering, the biochemical changes of hepatic lipogenesis in the genetic susceptibility to fatty liver were evaluated. These results showed that （1） the weight of fatty liver of the two breeds of geese were 801 and 375 g （P〈0.05）, respectively. There were no differences on the abdominal fat pat, filet total and filet pectoralis major in the two breeds experimental of the geese group （P〈0.05） and no difference on body and filet skin plus subcutaneous adipose tissue （P〉0.05） was found; （2） in these two breeds of geese, there were no differences on very-lowdensity lipoprotein （VLDL）, cholesteryl esters （CE） （P〈0.05）, free cholesterol （FC）, triglycerides （TG）, phospholipids （PL） and protein （P 〈 0.05）; （3） there were no differences on activities of malic enzyme （ME）, glucose-6-phosphatedehydrogenase （G6PDH）, acetyl-CoA-carboxylas （ACX）, fatty acid synthase （FAS）, and mRNA level of ME in the two experimental breeds of geese groups （P 〈0.05）; （4） test in Landaise geese group showed that there was no significant correlation with the specific enzymatic activities, while in Xupu geese group, the liver weight was negatively correlated to the specific activity of ACX and positively to that of ME; （5） in these overfed geese, ME activity appeared to be a major factor involved in the genetic susceptibility to hepatic steatosis and it determined the hepatic lipogenesis capacity.
文摘The effect of brown rice and corn-based diets on fatty liver weight, carcass traits, lipids accumulation and liver composition of Landes geese (Anser anser) after feeding the same amount of each diet was investigated. After 2 weeks overfeeding, besides body weight and body weight gain increased significantly, the fatty liver weights were increased dramatically from 94.67 to 554.67 and 754.00 g in corn treatment and rice treatment, respectively, and the accumulation lipids in adipose tissues reached up to or close to 2 folds of initial weight in both treatments. Liver weight was greater in brown rice group than in corn group (P<0.05). Compared to before overfeeding, triacylglycerols (TG) in geese fatty liver increased dramatically and predominated (from 9.08% to close to or more than 90% of hepatic lipids), whereas phospholipids (PL) and cholesteryl esters (CE) decreased sharply in both treatments (P<0.05). However, the percentages of lipids, TG and PL in fatty liver were higher in brown rice treatment than in corn treatment, and that of CE was lower (P<0.05). It indicates that overfeeding chiefly results in lipids accumulation in goose fatty liver and adipose tissues. Brown rice-based diet induces greater fatty liver, and brown rice is one of high quality overfeeding ingredients instead of corn.
基金supported by the French Foreign Office(International Research Extension Grant TASSILI 08MDU723)the Algerian Research Project(PNR,2011)
文摘Obesity, one of the most common health problems, is associated with glucose and lipid metabolism abnormalities, increased cardiovascular risk and oxidative stressI1}. Aging is also associated with an increase in oxidative stress[2]. Considering that older adults are already at risk for oxidant stress, the additional metabolic perturbations of obesity aggravated the susceptibility to oxidant stress. Modulating age-related oxidative stress via dietary interventions could reduce free radical production and significantly improve health in aging.
基金supported by a subtheme of the National Key Research and Development Program of China(2021YFD1300300)Construction of Peking Duck Industry Chain Standard Technical System for Geographical Indication of Beijing(11000024T 000002810090)+1 种基金the Beijing Innovation Consortium of Agriculture Research System(BAIC 06-2025)the Agricultural Science and Technology Innovation Program(ASTIP)of the Chinese Academy of Agricultural Sciences.
文摘This study explored the impact of astaxanthin(AST)supplementation on growth performance,serum lipid profile,gut morphology,and antioxidant and immune function in the intestinal mucosa of Pekin ducks subjected to overfeeding.A total of 150 male Pekin ducks at one day of age were randomly allotted into five treatment groups with five replicates of six ducks each.The control group and ad libitum group(ALG)received a basal diet while others received basal diets supplemented with AST at 40 mg/kg(Lowdose group[LDG]),80 mg/kg(medium-dose group[MDG]),and 120 mg/kg(high-dose group[HDG]).After 1 to 14 d on basal diets(brooding phase),the ducks were assigned to the dietary treatment groups for 15 to 38 d(Grower phase)and 39 to 42 d(overfeeding period).Results indicated that AST supplementation improved final body weight and weight gain at both the grower and overfeeding phases(P<0.05).Overfeeding increased the serum lipid level,altered intestinal morphology,and led to higher expression of pro-inflammatory factors and oxidative stress biomarkers while reducing antioxidant enzyme activity,associated gene expression,and anti-inflammatory factors in the duodenal and jejunal mucosa(P<0.05).Additionally,overfeeding caused increased apoptotic cell counts in the duodenal and jejunal mucosa of the control group(P<0.05),culminating in intestinal tissue damage and dysfunction.Dietary supplementation of AST mitigated these adverse effects,alleviated intestinal damage and promoted gut health.It exerted a hypolipidemic effect,improved villi morphometrics in the duodenum,jejunum,and ileum,and enhanced the levels of interleukin-4(IL-4),soluble tumor necrosis factor-alpha receptor(sTNFaR),and transforming growth factor-beta(TGF-b)(P<0.05).It also increased the activities of antioxidant enzymes and the mRNA expression of key antioxidant-related genes,including nuclear factor erythroid 2-related factor 2(Nrf2),glutathione S-transferases(GSTs),and glutamate-cysteine ligase catalytic subunit(GCLC)(P<0.05).Moreover,it reduced the expression of pro-inflammatory factors,oxidative stress biomarkers such as reactive oxygen species(ROS)and malondialdehyde(MDA),and the number of apoptotic cells in the duodenal and jejunal mucosa(P<0.05).Immunoglobulin secretion and mucosal immunity were also significantly improved with AST supplementation(P<0.05).Variations among the AST dietary groups suggest that a medium dosage of 80 mg/kg could effectively mitigate intestinal damage from overfeeding while enhancing growth performance,antioxidant defences,and immune responses.Our results would provide a theoretical reference for using AST as a nutritional strategy to enhance gut health in ducks exposed to overfeeding.
