Metal wear particles generated by the movement of joint prostheses inevitably lead to aseptic osteolytic damage and ultimately prosthesis loosening,which are aggravated by various types of regulated cell death of bone...Metal wear particles generated by the movement of joint prostheses inevitably lead to aseptic osteolytic damage and ultimately prosthesis loosening,which are aggravated by various types of regulated cell death of bone.Nevertheless,the exact cellular nature and regulatory network underlying osteoferroptosis are poorly understood.Here,we report that titanium particles(TP)induced severe peri-implant osteolysis and ferroptotic changes with concomitant transcriptional repression of a key anti-ferroptosis factor,GPX4,in a mouse model of calvarial osteolysis.GPX4 repression was accompanied by an increase in DNA methyltransferases(DNMTs)1/3a/3b and hypermethylation of the Gpx4 promoter,which were partly mediated by the transcriptional regulator/co-repressor KLF5 and NCoR.Conversely,treatment with SGI-1027,a DNMT-specific inhibitor,resulted in marked reversal of Gpx4 promoter hypermethylation and GPX4 repression,as well as improvement in ferroptotic osteolysis to a similar extent as with a ferroptosis inhibitor,liproxstatin-1.This suggests that epigenetic GPX4 repression and ferroptosis caused by the increase of DNMT1/3a/3b have a causal influence on TP-induced osteolysis.In cultured primary osteoblasts and osteoclasts,GPX4 repression and ferroptotic changes were observed primarily in osteoblasts that were alleviated by SGI-1027 in a GPX4 inactivation-sensitive manner.Furthermore,we developed a mouse strain with Gpx4 haplodeficiency in osteoblasts(Gpx4^(0b+/-))thatexhibited worsened ferroptotic osteolysis in control and TP-treated calvaria and largely abolished the anti-ferroptosis and osteoprotective effects of SGl-1027.Taken together,our results demonstrate that DNMT1/3a/3b elevation,resulting GPX4 repression,and osteoblastic ferroptosis form a critical epigenetic pathway that significantly contributes to TP-induced osteolysis,and that targeting DNMT aberration and the associated osteoferroptosis could be a potential strategy to prevent or slow down prosthesis-related osteolytic complications.展开更多
基金supported by the National Major Research Plan of National Natural and Science Foundation of China(NSFC)(92368201)National Key Research and Development Project(2021YFA1201404)+4 种基金Major Project of NSFC(81991514)NSFC General Program(81970577)Jiangsu Provincial Key Medical Center Foundation,Jiangsu Province Medical Innovation Center of Orthopedic Surgery(CXZX-202214)Jiangsu Provincial Medical Youth Talent Foundation,Jiangsu Provincial Medical Outstanding Talent Foundation,Jiangsu Provincial Key Medical Talent Foundationthe Fundamental Research Funds for the Central Universities(14380493 and 14380494)。
文摘Metal wear particles generated by the movement of joint prostheses inevitably lead to aseptic osteolytic damage and ultimately prosthesis loosening,which are aggravated by various types of regulated cell death of bone.Nevertheless,the exact cellular nature and regulatory network underlying osteoferroptosis are poorly understood.Here,we report that titanium particles(TP)induced severe peri-implant osteolysis and ferroptotic changes with concomitant transcriptional repression of a key anti-ferroptosis factor,GPX4,in a mouse model of calvarial osteolysis.GPX4 repression was accompanied by an increase in DNA methyltransferases(DNMTs)1/3a/3b and hypermethylation of the Gpx4 promoter,which were partly mediated by the transcriptional regulator/co-repressor KLF5 and NCoR.Conversely,treatment with SGI-1027,a DNMT-specific inhibitor,resulted in marked reversal of Gpx4 promoter hypermethylation and GPX4 repression,as well as improvement in ferroptotic osteolysis to a similar extent as with a ferroptosis inhibitor,liproxstatin-1.This suggests that epigenetic GPX4 repression and ferroptosis caused by the increase of DNMT1/3a/3b have a causal influence on TP-induced osteolysis.In cultured primary osteoblasts and osteoclasts,GPX4 repression and ferroptotic changes were observed primarily in osteoblasts that were alleviated by SGI-1027 in a GPX4 inactivation-sensitive manner.Furthermore,we developed a mouse strain with Gpx4 haplodeficiency in osteoblasts(Gpx4^(0b+/-))thatexhibited worsened ferroptotic osteolysis in control and TP-treated calvaria and largely abolished the anti-ferroptosis and osteoprotective effects of SGl-1027.Taken together,our results demonstrate that DNMT1/3a/3b elevation,resulting GPX4 repression,and osteoblastic ferroptosis form a critical epigenetic pathway that significantly contributes to TP-induced osteolysis,and that targeting DNMT aberration and the associated osteoferroptosis could be a potential strategy to prevent or slow down prosthesis-related osteolytic complications.
