Objectiye To eiucidate the inducing factor in osteogenesis piayed by dermai fibroblasts. mthods Fibroblasts and epithelial cells were procured from rabbit and human skins and separated. These two types of cells were t...Objectiye To eiucidate the inducing factor in osteogenesis piayed by dermai fibroblasts. mthods Fibroblasts and epithelial cells were procured from rabbit and human skins and separated. These two types of cells were then cultured either together or separately and studied histochemically, immunohistochemically and biochemically. Results Bone nodules were found only in the mtxed culture of fibroblasts and epithelial cells. During the period ol exuberant bone nodule formation, there were both more positive keratin- stained cells (epithelial cells) and higher ALP activity. When few or no bone nodules formed, positive keratin- stained cells declined or disappeared and the ALP activity fell off. Conclusion That dermal fibroblasts show their osteogenic potential depends mainly on the inducing effect of the ePithelial cells.展开更多
A specific peptide(EEEFDATR)that is released during the hydrolysis of Antarctic krill contains three glutamic acids that cover calcium-binding ligands,and might act as a potential role in enhancing the osteogenetic ac...A specific peptide(EEEFDATR)that is released during the hydrolysis of Antarctic krill contains three glutamic acids that cover calcium-binding ligands,and might act as a potential role in enhancing the osteogenetic activity.In this study,EEEFDATR and calcium alone and in combination were examined for their effects on the proliferation,differentiation,and mineralization of MC3T3-E1 osteoblastic cells.Co-administration of CaCl_(2)and EEEFDATR showed a superior proliferation rate(70.94%)of MC3T3-E1 cells,which was about 2.07 and 1.74 times higher than CaCl_(2)and EEEFDATR alone,respectively.Osteoblast proliferation improvements were associated with increased cell cycle in S phase after co-administration of CaCl_(2)and EEEFDATR.The CaCl_(2)+EEEFDATR group further exhibited a significantly higher alkaline phosphatase(ALP)activity as compared to other groups(p<0.05)and showed obvious differentiation of MC3T3-E1 cells.The magnitude of mineralization in the extracellular matrix(calcium deposition)was also higher in MC3T3-E1 cells treated with combined administration,although EEEFDATR alone did not induce significant mineralization.These data suggest that co-administration of CaCl_(2)and EEEFDATR was superior to mono-administration in improving osteogenetic activity.展开更多
To endow Ti-based orthopedic implants immunomodulatory capability and thus enhanced osseointegration,different amounts of Sr are doped in Na_(2)TiO_(3) nanorods in the arrays with identical nanotopographic parameters(...To endow Ti-based orthopedic implants immunomodulatory capability and thus enhanced osseointegration,different amounts of Sr are doped in Na_(2)TiO_(3) nanorods in the arrays with identical nanotopographic parameters(rod diameter,length and inter-rod spacing)by substitution of Na^(+) using hydrothermal treatment.The obtained arrays are denoted as STSr2,STSr4,and STSr7,where the arabic numbers indicate the incorporating amounts of Sr in Na_(2)TiO_(3).The modulation effects of the Sr-doped nanorods arrays on macrophage polarization and osteogenetic functions of osteoblasts are investigated,together with the array without Sr(ST).Moreover,osseointegration of these arrays are also assayed in rat femoral condyles.Sr-doped nanorods arrays accelerate M1(pro-inflammatory phenotype)-to-M2(anti-inflammatory phenotype)transformation of the adhered macrophages,enhancing secretion of pro-osteogenetic cytokines and growth factors(TGF-β1 and BMP2),moreover,the Sr doped arrays directly enhance osteogenetic functions of osteoblasts.The enhancement of paracrine of M2 macrophages and osteogenetic function of osteoblasts is promoted with the increase of Sr incorporating amounts.Consequently,Sr doped arrays show significantly enhanced osseointegration in vivo compared to ST,and STSr7 exhibits the best performance.Our work sheds a new light on the design of surface chemical components and structures for orthopedic implants to enhance their osseointegration.展开更多
文摘Objectiye To eiucidate the inducing factor in osteogenesis piayed by dermai fibroblasts. mthods Fibroblasts and epithelial cells were procured from rabbit and human skins and separated. These two types of cells were then cultured either together or separately and studied histochemically, immunohistochemically and biochemically. Results Bone nodules were found only in the mtxed culture of fibroblasts and epithelial cells. During the period ol exuberant bone nodule formation, there were both more positive keratin- stained cells (epithelial cells) and higher ALP activity. When few or no bone nodules formed, positive keratin- stained cells declined or disappeared and the ALP activity fell off. Conclusion That dermal fibroblasts show their osteogenic potential depends mainly on the inducing effect of the ePithelial cells.
基金supported by the LiaoNing Revitalization Talents Program of China(XLYC2007078)the Program for Innovative Talents in Higher Education of Liaoning Province of China(LR2019008).
文摘A specific peptide(EEEFDATR)that is released during the hydrolysis of Antarctic krill contains three glutamic acids that cover calcium-binding ligands,and might act as a potential role in enhancing the osteogenetic activity.In this study,EEEFDATR and calcium alone and in combination were examined for their effects on the proliferation,differentiation,and mineralization of MC3T3-E1 osteoblastic cells.Co-administration of CaCl_(2)and EEEFDATR showed a superior proliferation rate(70.94%)of MC3T3-E1 cells,which was about 2.07 and 1.74 times higher than CaCl_(2)and EEEFDATR alone,respectively.Osteoblast proliferation improvements were associated with increased cell cycle in S phase after co-administration of CaCl_(2)and EEEFDATR.The CaCl_(2)+EEEFDATR group further exhibited a significantly higher alkaline phosphatase(ALP)activity as compared to other groups(p<0.05)and showed obvious differentiation of MC3T3-E1 cells.The magnitude of mineralization in the extracellular matrix(calcium deposition)was also higher in MC3T3-E1 cells treated with combined administration,although EEEFDATR alone did not induce significant mineralization.These data suggest that co-administration of CaCl_(2)and EEEFDATR was superior to mono-administration in improving osteogenetic activity.
基金the National Natural Science Foundation of China(Grant number 51631007,51971171 and 31700860)the joint project of Xi’an Jiaotong University and Beijing Research Institute of Traumatology and Orthopaedics(Contract No.202012443)for financially supporting this work.
文摘To endow Ti-based orthopedic implants immunomodulatory capability and thus enhanced osseointegration,different amounts of Sr are doped in Na_(2)TiO_(3) nanorods in the arrays with identical nanotopographic parameters(rod diameter,length and inter-rod spacing)by substitution of Na^(+) using hydrothermal treatment.The obtained arrays are denoted as STSr2,STSr4,and STSr7,where the arabic numbers indicate the incorporating amounts of Sr in Na_(2)TiO_(3).The modulation effects of the Sr-doped nanorods arrays on macrophage polarization and osteogenetic functions of osteoblasts are investigated,together with the array without Sr(ST).Moreover,osseointegration of these arrays are also assayed in rat femoral condyles.Sr-doped nanorods arrays accelerate M1(pro-inflammatory phenotype)-to-M2(anti-inflammatory phenotype)transformation of the adhered macrophages,enhancing secretion of pro-osteogenetic cytokines and growth factors(TGF-β1 and BMP2),moreover,the Sr doped arrays directly enhance osteogenetic functions of osteoblasts.The enhancement of paracrine of M2 macrophages and osteogenetic function of osteoblasts is promoted with the increase of Sr incorporating amounts.Consequently,Sr doped arrays show significantly enhanced osseointegration in vivo compared to ST,and STSr7 exhibits the best performance.Our work sheds a new light on the design of surface chemical components and structures for orthopedic implants to enhance their osseointegration.
