Objective:This study aims to investigate the regulatory effects of piezoelectricity on the inflammatory microenvironment in osteoporosis treatment.Impact Statement:Recent studies have extensively explored the impact o...Objective:This study aims to investigate the regulatory effects of piezoelectricity on the inflammatory microenvironment in osteoporosis treatment.Impact Statement:Recent studies have extensively explored the impact of piezoelectric materials on macrophage polarization and cytokine secretion.However,the effects of piezoelectricity on macrophages for the regulation of immune osteogenesis in osteoporosis remain poorly understood.This study provides novel insights into the regulatory role of piezoelectricity in macrophage modulation within osteoporotic diseases.Introduction:The overexpression of various inflammatory factors in osteoporosis exacerbates bone metabolism imbalance.Macrophage polarization plays a pivotal role in inflammation regulation and tissue repair.Therefore,investigating the regulatory effects of piezoelectricity on macrophage polarization is crucial for improving the inflammatory microenvironment and fostering an immune environment conducive to osteoporotic bone regeneration.Methods:This study fabricated polarized potassium sodium niobate ceramic(PKNN)piezoelectric bioceramics using solid-phase sintering and high-pressure polarization techniques,and investigated their regulatory effects on macrophage polarization,anti-inflammatory factor expression,and osteogenic differentiation bone marrow mesenchymal stem cells derived from ovariectomized rats(rBMSCs-OVX).Results:PKNN substantially promotes M2 macrophage polarization and enhances anti-inflammatory factor expression,effectively suppressing inflammatory responses.Further studies demonstrate that PKNN,by modulating macrophages,indirectly regulates osteoblast gene expression,improving the inhibitory effects of the pathological microenvironment on osteogenic differentiation of rBMSCs-OVX.Conclusion:This research provides important theoretical evidence for the development of immunomodulatory osteoporotic bone regeneration materials driven by piezoelectricity.展开更多
Objective:This work aims to construct a functional titanium surface with spontaneous electrical stimulation for immune osteogenesis and antibacteria.Impact Statement:A silver–calcium microgalvanic cell was engineered...Objective:This work aims to construct a functional titanium surface with spontaneous electrical stimulation for immune osteogenesis and antibacteria.Impact Statement:A silver–calcium microgalvanic cell was engineered on the titanium implant surface to spontaneously generate microcurrents for osteoimmunomodulation and bacteria killing,which provides a promising strategy for the design of a multifunctional electroactive titanium implant.Introduction:Titanium-based implants are usually bioinert,which often leads to inflammation-induced loosening.Electrical stimulation has therapeutic potential;however,its dependence on external devices limits its clinical application.Therefore,designing an electroactive titanium surface with endogenous electrical stimulation capability is a promising strategy to overcome implant failure induced by inflammation.Methods:The silver–calcium micro-galvanic cell was constructed on titanium substrate surfaces by the ion implantation technique.RAW264.7 and MC3T3-E1 were used for cell culture studies with the material to evaluate immunomodulatory and osteogenic abilities of the implant.The expression levels of inflammatory genes and voltage-gated Ca2+channel-related genes were tested for investigating the mechanism of immunoregulation.The antibacterial properties of the modified titanium were assessed.Finally,its immunomodulatory effects in vivo were verified by a mouse subcutaneous inflammation model.Results:The silver–calcium micro-galvanic modified titanium surface generates microcurrents and releases Ca^(2+),which induces macrophage polarization toward the M2 phenotype and promotes osteogenic differentiation via paracrine signaling,exhibiting excellent antibacterial activity.Conclusion:The silver–calcium micro-galvanic cell on titanium could regulate the immune response to promote bone repair and exhibit antibacterial capabilities through noninvasive electrical stimulation,providing a promising strategy for the design of multifunctional electroactive implant surfaces.展开更多
Bone defects have serious economic and clinical impacts;however,despite improvements in bone defect management,the range of clinical outcomes remains limited.A variety of biomaterials have been used to treat complex b...Bone defects have serious economic and clinical impacts;however,despite improvements in bone defect management,the range of clinical outcomes remains limited.A variety of biomaterials have been used to treat complex bone defects.However,final bone repair outcomes may be adversely affected by poor osteogenic capacity and risk of infection.Consequently,therapeutic methods are required that reduce bacterial contamination and increase the use of osteogenic biomaterials.Herein,we report the preparation of poly(lactic acid-coglycolic acid)(PLGA)microspheres coloaded with magnesium(Mg^(2+))and gallium(Ga^(3+))ions(Mg-Ga@PLGA),which can fill irregular bone defects and show good biosafety.During in vitro testing,Mg-Ga@PLGA not only showed a synergistic effect on promoting osteogenic differentiation but also inhibited osteoclastic differentiation.Moreover,we found that Mg-Ga@PLGA demonstrated an antibacterial effect.During in vivo testing,Mg Ga@PLGA exhibited strong in situ osteogenic ability.In conclusion,Mg-Ga@PLGA has good potential for treating bone defects at risk of infection.展开更多
Osteogenesis is the process of bone formation mediated by the osteoblasts,participating in various bone-related physiological processes including bone development,bone homeostasis and fracture healing.It exhibits temp...Osteogenesis is the process of bone formation mediated by the osteoblasts,participating in various bone-related physiological processes including bone development,bone homeostasis and fracture healing.It exhibits temporal and spatial interconnectivity with angiogenesis,constructed by multiple forms of cell communication occurring between bone and vascular endothelial cells.Molecular regulation among different cell types is crucial for coordinating osteogenesis and angiogenesis to facilitate bone remodeling,fracture healing,and other bone-related processes.The transmission of signaling molecules and the activation of their corresponding signal pathways are indispensable for various forms of cell communication.This communication acts as a“bridge”in coupling osteogenesis to angiogenesis.This article reviews the modes and processes of cell communication in osteogenesisangiogenesis coupling over the past decade,mainly focusing on interactions among bone-related cells and vascular endothelial cells to provide insights into the mechanism of cell communication of osteogenesis-angiogenesis coupling in different bone-related contexts.Moreover,clinical relevance and applications are also introduced in this review.展开更多
Guided bone regeneration(GBR)membranes are extensively utilized in dental implantation.However,the existing GBR membranes showed insufficient space-maintaining capability and poor bone promoting ability,affecting the ...Guided bone regeneration(GBR)membranes are extensively utilized in dental implantation.However,the existing GBR membranes showed insufficient space-maintaining capability and poor bone promoting ability,affecting the effectiveness of clinical bone augmentation,which in turn resulted in poor implant outcomes and even failure.In this study,we designed a novel magnesium reinforced sandwich structured composite membrane,consisting of an inner magnesium scaffold and a PLGA/collagen hybrid(mixture of poly(lactic-co-glycolic acid)and collagen)top and bottom layer.The magnesium scaffold provided mechanical support and released Mg^(2+)to enhance osteogenesis.The PLGA/collagen hybrid regulated membrane degradation and improved biocompatibility,promoting cell adhesion and proliferation(P<0.05).The PLGA/collagen hybrid regulated the release of magnesium ions,such that the MgP10C(mass ratios of PLGA and collagen=100:10)group showed the best in vitro osteogenic effect.Further mechanism exploration confirmed that MgP10C membranes significantly enhanced bone defect repair via the MAPK/ERK 1/2 pathway by the Mg^(2+)released from the composite membranes.In rat calvarial defect and rabbit alveolar defect model(P<0.05),the in vivo osteogenic effect of the MgP10C group was superior to that of other groups.Finite element analysis models validated the support effect of composite membranes,demonstrating lower stress and a significant reduction in strain on the bone graft in the MgP10C group.In conclusion,the magnesium-reinforced sandwich structure composite membrane,with its spacemaintaining properties and osteoinductive activity,represents a new strategy for GBR and enhancing osteogenic potential that meets directly clinical needs.展开更多
BACKGROUND Metabolic-associated fatty liver disease(MAFLD)is characterized by lipid accumulation in hepatocytes and is closely associated with oxidative stress.Increasing clinical evidence indicates that MAFLD is link...BACKGROUND Metabolic-associated fatty liver disease(MAFLD)is characterized by lipid accumulation in hepatocytes and is closely associated with oxidative stress.Increasing clinical evidence indicates that MAFLD is linked to bone metabolic disorders,including osteoporosis.Recent studies indicate that the expression profiles of liver circular RNAs(circRNAs)are altered in MAFLD.However,the effects of these changes on bone metabolism remain poorly understood.AIM To investigate the effects and mechanism of differently expressed circRNAs secreted by the liver on osteogenic differentiation in MAFLD.METHODS RNA sequencing was performed to identify highly expressed circRNAs in the liver,validated by quantitative real-time reverse transcription polymerase chain reaction,and localized using fluorescence in situ hybridization(FISH).A mouse model induced by a high-fat diet was used to simulate MAFLD.RESULTS CircSOD2 was significantly upregulated in liver tissues and primary hepatocytes from subjects with MAFLD.CircSOD2 was induced by oxidative stress and attenuated by antioxidants in the mouse model.In addition,circSOD2 was delivered from hepatocytes to bone marrow mesenchymal stem cells(BMSCs).Furthermore,circSOD2 inhibited the osteogenic differentiation of BMSCs and in vivo bone formation by sponging miR-29b.Moreover,miR-29b inhibition reversed the stimulatory effect of circSOD2 silencing on osteogenic differentiation of BMSCs and in vivo bone formation.Mechanistically,the interaction between circSOD2 and miR-29b confirmed through a luciferase reporter assay and the co-localization in the cytoplasm evidenced by FISH indicated that circSOD2 acted as a sponge for miR-29b.CONCLUSION This study provides a novel mechanism underlying the liver-bone crosstalk,demonstrating that circSOD2 upregulation in hepatocytes,induced by oxidative stress,inhibits osteogenic differentiation of BMSCs by sponging miR-29b.These findings offer a better understanding of the relationship between MAFLD and osteoporosis.展开更多
Steroid-induced osteonecrosis of the femoral head (SONFH) is a severe bone disorder that was clinically treated by core decompression (CD) combined with bone grafting.However,the clinical outcome of this therapy for S...Steroid-induced osteonecrosis of the femoral head (SONFH) is a severe bone disorder that was clinically treated by core decompression (CD) combined with bone grafting.However,the clinical outcome of this therapy for SONFH is usually unsatisfactory.Magnesium (Mg) is a biodegradable material reported as an ideal orthopedic implant.The Mg^(2+)released from its degradation is considered an effective factor for bone tissue formation,but the rapid degradation rate limited its application.Here,we reported a magnesiumDscandium (MgDSc) alloy for promoting bone regeneration in SONFH.We discovered that MgDSc alloy with satisfied corrosion resistance,antiapoptosis,angiogenic,and osteogenic properties in vitro,could facilitate osteogenic differentiation by activating the Wnt/β-catenin signaling pathway.In the clinically relevant SONFH model,CD combined with suitable MgDSc alloy implantation promoted angiogenesisDosteogenesis coupling and alleviated osteonecrosis.We observed fewer apoptotic osteocytes and empty bone lacunae,but more blood vessels and new bone formation in the Mg-Sc alloy implanted CD tunnel region compared with CD-treated SONFH rabbits.Properly degraded MgDSc alloy maintains high structural integrity and provides reliable mechanical support for the osteonecrotic femoral head,which is conducive to further clinical translation.展开更多
Objective:This study aims to clarify the effects of bioceramic interface cues on macrophages.Impact Statement:Recently,there have been many researches exploring the effects of interface topography cues on macrophage p...Objective:This study aims to clarify the effects of bioceramic interface cues on macrophages.Impact Statement:Recently,there have been many researches exploring the effects of interface topography cues on macrophage polarization and cytokine secretion.However,the effects and underlying mechanisms of bioceramic interface cues on macrophages still need exploring.This study provides insights into the effects of bioceramic micro-groove surface structures on macrophages.Introduction:With the development of bone tissue engineering methods,bioceramics have been used for bone repair.After the implantation of bioceramics,innate immune response that occurs at the interface of materials can deeply influence the subsequent inflammation and bone regeneration progress.Therefore,the exploration and regulation of immune response of the bioceramic interface will be beneficial to promote the bone regeneration effects.Methods:In this study,bioceramics with micro-groove structures on the surface are fabricated by digital light processing 3-dimensional printing technology.Then,micro-groove structures with different spacings(0,25,50,and 75μm)are prepared separately to explore the effects on macrophages.Results:The large spacing micro-groove structure can promote the M2 polarization and osteoinductive cytokine secretion of macrophage.The reason is that the large spacing micro-groove structure can induce directional arrangement of macrophage so as to change the phenotype and cytokine secretion.Further researches show that macrophage of the large spacing micro-groove structure can promote the osteogenic differentiation of bone mesenchymal stem cells,which can benefit osteogenesis and osteointegration.Conclusion:This study offers an effective and application potential method for bone repair.展开更多
Magnesium(Mg)alloys with biodegradability and excellent mechanical properties are in high demand for applications in guided bone regeneration(GBR).However,the clinical application of Mg alloys is hindered by infection...Magnesium(Mg)alloys with biodegradability and excellent mechanical properties are in high demand for applications in guided bone regeneration(GBR).However,the clinical application of Mg alloys is hindered by infection risks and limited osteogenesis.Herein,a structure-functional integrated Mg-Ca/Mg-Cu bilayer membrane was rolled at 150℃through various single-pass reductions by using online heating rolling.The Mg-Cu layer was specifically engineered to exhibit antibacterial properties tailored for gingival tissue,while the Mg-Ca layer was designed to support bone regeneration within the defect cavity.The bilayer membrane demonstrated a flexural yield strength of 421.0 MPa and a modulus of 58.6 GPa,indicating exceptional deformation resistance.Furthermore,it maintained notable structural stability by retaining 86.4%of its volume after 21 days in Hanks'solution.In vitro results revealed that the bilayer membrane exhibited favorable biocompatibility and promoted osteogenesis via the synergetic effect of released Mg^(2+)and Ca^(2+)ions.The rapid release of Cu^(2+)ions and the creation of an alkaline environment further improved antibacterial properties,potentially preventing postoperative infections.Additionally,in an in vivo rat calvarial defect model,the membrane demonstrated its capability to stimulate new bone formation.In summary,the Mg-Ca/Mg-Cu bilayer membrane exhibited outstanding mechanical stability,favorable corrosion rates,extraordinary osteogenic and antibacterial activity simultaneously.Consequently,it holds promise as a robust barrier membrane in GBR applications.展开更多
Mimicking the electric microenvironment of natural tissue is a promising strategy for developing biomedical implants. However, current research has not taken biomimetic electrical functional units into consideration w...Mimicking the electric microenvironment of natural tissue is a promising strategy for developing biomedical implants. However, current research has not taken biomimetic electrical functional units into consideration when designing biomedical implants. In this research, ordered structures with Schottky heterojunction functional unit (OSSH) were constructed on titanium implant surfaces for bone regeneration regulation. The Schottky heterojunction functional unit is composed of periodically distributed titanium microdomain and titanium oxide microdomain with different carrier densities and surface potentials. The OSSH regulates the M2-type polarization of macrophages to a regenerative immune response by activating the PI3K-AKT-mTOR signal pathway and further promotes osteogenic differentiation of rat bone marrow mesenchymal stem cells. This work provides fundamental insights into the biological effects driven by the Schottky heterojunction functional units that can electrically modulate osteogenesis.展开更多
Bone is a highly calcified and vascularized tissue.The vascular system plays a vital role in supporting bone growth and repair,such as the provision of nutrients,growth factors,and metabolic waste transfer.Moreover,th...Bone is a highly calcified and vascularized tissue.The vascular system plays a vital role in supporting bone growth and repair,such as the provision of nutrients,growth factors,and metabolic waste transfer.Moreover,the additional functions of the bone vasculature,such as the secretion of various factors and the regulation of bone-related signaling pathways,are essential for maintaining bone health.In the bone microenvironment,bone tissue cells play a critical role in regulating angiogenesis,including osteoblasts,bone marrow mesenchymal stem cells(BMSCs),and osteoclasts.Osteogenesis and bone angiogenesis are closely linked.The decrease in osteogenesis and bone angiogenesis caused by aging leads to osteoporosis.Long noncoding RNAs(lncRNAs)are involved in various physiological processes,including osteogenesis and angiogenesis.Recent studies have shown that lncRNAs could mediate the crosstalk between angiogenesis and osteogenesis.However,the mechanism by which lncRNAs regulate angiogenesis-osteogenesis crosstalk remains unclear.In this review,we describe in detail the ways in which lncRNAs regulate the crosstalk between osteogenesis and angiogenesis to promote bone health,aiming to provide new directions for the study of the mechanism by which lncRNAs regulate bone metabolism.展开更多
Osteogenesis imperfecta(OI)is a group of diseases caused by defects in type I collagen processing which result in skeletal fragility.While these disorders have been regarded as defects in osteoblast function,the role ...Osteogenesis imperfecta(OI)is a group of diseases caused by defects in type I collagen processing which result in skeletal fragility.While these disorders have been regarded as defects in osteoblast function,the role of matrix-embedded osteocytes in OI pathogenesis remains largely unknown.Homozygous human SP7(c.946 C>T,R316C)mutation results in a recessive form of OI characterized by fragility fractures,low bone mineral density and osteocyte dendrite defects.To better understand how the OI-causing R316C mutation affects the function of SP7,we generated Sp7^(R342C)knock-in mice.Consistent with patient phenotypes,Sp7^(R342C/R342C)mice demonstrate increased cortical porosity and reduced cortical bone mineral density.Sp7^(R342C/R342C)mice show osteocyte dendrite defects,increased osteocyte apoptosis,and intracortical bone remodeling with ectopic intracortical osteoclasts and elevated osteocyte Tnfsf11 expression.展开更多
Insufficient alveolar bone thickness increases the risk of periodontal dehiscence and fenestration,especially in orthodontic tooth movement.Abaloparatide(ABL),a synthetic analog of human PTHr P(1–34)and a clinical me...Insufficient alveolar bone thickness increases the risk of periodontal dehiscence and fenestration,especially in orthodontic tooth movement.Abaloparatide(ABL),a synthetic analog of human PTHr P(1–34)and a clinical medication for treating osteoporosis,has recently demonstrated its potential in enhancing craniofacial bone formation.Herein,we show that intraoral submucosal injection of ABL,when combined with mechanical force,promotes in situ alveolar bone thickening.The newly formed bone is primarily located outside the original compact bone,implying its origin from the periosteum.RNA sequencing of the alveolar bone tissue revealed that the focal adhesion(FA)pathway potentially mediates this bioprocess.Local injection of ABL alone enhances cell proliferation,collagen synthesis,and phosphorylation of focal adhesion kinase(FAK)in the alveolar periosteum;when ABL is combined with mechanical force,the FAK expression is upregulated,in line with the accomplishment of the ossification.In vitro,ABL enhances proliferation,migration,and FAK phosphorylation in periosteal stem cells.Furthermore,the pro-osteogenic effects of ABL on alveolar bone are entirely blocked when FAK activity is inhibited by a specific inhibitor.In summary,abaloparatide combined with mechanical force promotes alveolar bone formation via FAK-mediated periosteal osteogenesis.Thus,we have introduced a promising therapeutic approach for drug-induced in situ alveolar bone augmentation,which may prevent or repair the detrimental periodontal dehiscence,holding significant potential in dentistry.展开更多
Aim Understanding the response of mesenchymal stem cells （MSCs） to mechanical strain and their consequent gene expression patterns will broaden our knowledge of the mechanobiology of distraction osteogenesis. Method...Aim Understanding the response of mesenchymal stem cells （MSCs） to mechanical strain and their consequent gene expression patterns will broaden our knowledge of the mechanobiology of distraction osteogenesis. Methodology In this study, a single period of cyclic mechanical stretch （0.5 Hz, 2,000 με） was performed on rat bone marrow MSCs. Cellular proliferation and alkaline phosphatase （ALP） activity was examined. The mRNA expression of six bone-related genes （Ets-1, bFGF, IGF-Ⅱ, TGF-β, Cbfal and ALP） was detected using real-time quantitative RT-PCR. Results The results showed that mechanical strain can promote MSCs proliferation, increase ALP activity, and up-regulate the expression of these genes. A significant increase in Ets-1 expression was detected immediately after mechanical stimulation, but Cbfal expression became elevated later. The temporal expression pattem of ALP coincided perfectly with Cbfal. Conclusion The results of this study suggest that mechanical strain may act as a stimulator to induce differentiation of MSCs into osteoblasts, and that these bone-related genes may play different roles in the response of MSCs to mechanical stimulation.展开更多
Age related defect of the osteogenic differentiation of mesenchymal stem cells（MSCs） plays a key role in osteoporosis. Mechanical loading is one of the most important physical stimuli for osteoblast differentiation....Age related defect of the osteogenic differentiation of mesenchymal stem cells（MSCs） plays a key role in osteoporosis. Mechanical loading is one of the most important physical stimuli for osteoblast differentiation.Here, we compared the osteogenic potential of MSCs from young and adult rats under three rounds of 2 h of cyclic stretch of 2.5% elongation at 1 Hz on 3 consecutive days. Cyclic stretch induced a significant osteogenic differentiation of MSCs from young rats, while a compromised osteogenesis in MSCs from the adult rats.Accordingly, there were much more reactive oxygen species（ROS） production in adult MSCs under cyclic stretch compared to young MSCs. Moreover, ROS scavenger N-acetylcysteine rescued the osteogenic differentiation of adult MSCs under cyclic stretch. Gene expression analysis revealed that superoxide dismutase 1（SOD1） was significantly downregulated in those MSCs from adult rats. In summary, our data suggest that reduced SOD1 may result in excessive ROS production in adult MSCs under cyclic stretch, and thus manipulation of the MSCs from the adult donors with antioxidant would improve their osteogenic ability.展开更多
Objective： We investigated the effects of intermittent negative pressure on osteogenesis in human bone marrowderived stroma cells （BMSCs） in vitro. Methods： BMSCs were isolated from adult marrow donated by a hip o...Objective： We investigated the effects of intermittent negative pressure on osteogenesis in human bone marrowderived stroma cells （BMSCs） in vitro. Methods： BMSCs were isolated from adult marrow donated by a hip osteoarthritis patient with prosthetic replacement and cultured in vitro. The third passage cells were divided into negative pressure treatment group and control group. The treatment group was induced by negative pressure intermittently （pressure： 50 kPa, 30 min/times, and twice daily）. The control was cultured in conventional condition. The osteogenesis of BMSCs was examined by phase-contrast mi- croscopy, the determination of alkaline phosphatase （ALP） activities, and the immunohistochemistry of collagen type 1. The mRNA expressions of osteoprotegerin （OPG） and osteoprotegerin ligand （OPGL） in BMSCs were analyzed by real-time polymerase chain reaction （PCR）. Results： BMSCs showed a typical appearance of osteoblast after 2 weeks of induction by intermittent negative pressure, the activity of ALP increased significantly, and the expression of collagen type I was positive. In the treatment group, the mRNA expression of OPG increased significantly （P〈0.05） and the mRNA expression of OPGL decreased significantly （P〈0.05） after 2 weeks, compared with the control. Conclusion： Intermittent negative pressure could promote osteogenesis in human BMSCs in vitro.展开更多
YAP（yes-associated protein） is a transcriptional factor that is negatively regulated by Hippo pathway, a conserved pathway for the development and size control of multiple organs. The exact function of YAP in bone h...YAP（yes-associated protein） is a transcriptional factor that is negatively regulated by Hippo pathway, a conserved pathway for the development and size control of multiple organs. The exact function of YAP in bone homeostasis remains controversial. Here we provide evidence for YAP＇s function in promoting osteogenesis, suppressing adipogenesis, and thus maintaining bone homeostasis.YAP is selectively expressed in osteoblast（OB）-lineage cells. Conditionally knocking out Yap in the OB lineage in mice reduces cell proliferation and OB differentiation and increases adipocyte formation, resulting in a trabecular bone loss. Mechanistically, YAP interacts with β-catenin and is necessary for maintenance of nuclear β-catenin level and Wnt/β-catenin signaling. Expression of β-catenin in YAP-deficient BMSCs（bone marrow stromal cells） diminishes the osteogenesis deficit. These results thus identify YAP-β-catenin as an important pathway for osteogenesis during adult bone remodeling and uncover a mechanism underlying YAP regulation of bone homeostasis.展开更多
Attempt of developing bio-safety and functional layered double hydroxides(LDHs)modifed plasma electrolytic oxidation(PEO)coatings,has become a hotspot in the protection of magnesium(Mg)based biomedical implants.In the...Attempt of developing bio-safety and functional layered double hydroxides(LDHs)modifed plasma electrolytic oxidation(PEO)coatings,has become a hotspot in the protection of magnesium(Mg)based biomedical implants.In the present work,Mg-Fe LDH flms with different Fe contents were fabricated on PEO coating via a novel two-step method:frstly,the Fe OOH flms were prepared by immersing PEO sample in Fe^(2+)-containing solution,and then Mg-Fe LDH flms were formed by transforming the Fe OOH flms via a hydrothermal treatment in water.The highly-oriented LDH nano-sheets could enhance the anti-corrosion performance of PEO coating,which was proved by the results of electrochemical test,hydrogen evolution and corroded morphology.PEO/Mg-Fe LDH coatings showed a low hemolysis rate(less5%)than PEO coating.In addition,PEO/Mg-Fe LDH coatings were more favorable for cell adhesion and proliferation than PEO coating.Moreover,PEO/Mg-Fe LDH coatings showed good photothermal conversion property,which demonstrated the excellent rapid antibacterial effect under NIR light.In vitro culture of rat bone marrow stem cell(r BMSC)suggested that cells cultured in the extract of PEO/Mg-Fe LDH coatings had a better osteogenic activity.In vivo subcutaneous implantation test revealed that PEO/Mg-Fe LDH coatings exhibited good anti-corrosion and histocompatibility.展开更多
Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system. However, there is little study on the osteogenesis and angiogenesis effect of Gins...Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system. However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1. Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive. In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly. Then,micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively. It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression. Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1. Significantly, Ginsenoside Rb1 loaded on micro-nano HAp/silk could facilitate osteogenesis and angiogenesis. All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor’s expression of BMSCs. Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.展开更多
Poly methyl methacrylate(PMMA)bone cement is used in augmenting and stabilizing fractured vertebral bodies through percutaneous vertebroplasty(PVP)and percutaneous kyphoplasty(PKP).However,applications of PMMA bone ce...Poly methyl methacrylate(PMMA)bone cement is used in augmenting and stabilizing fractured vertebral bodies through percutaneous vertebroplasty(PVP)and percutaneous kyphoplasty(PKP).However,applications of PMMA bone cement are limited by the high elasticity modulus of PMMA,its low biodegradability,and its limited ability to regenerate bone.To improve PMMA bio activity and biodegradability and to modify its elasticity modulus,we mixed PMMA bone cement with oxidized hyaluronic acid and carboxymethyl chitosan in situ cross-linking hydrogel loaded with bone morphogenetic protein-2(BMP-2)to achieve novel hybrid cement.These fabric ated PMMA-hydrogel hybrid cements exhibited lower setting temperatures,a lower elasticity modulus,and better biodegradability and biocompatibility than that of pure PMMA cement,while retaining acceptable setting times,mechanical strength,and inj ectability.In addition,we detected release of BMP-2 from the PMMA-hydrogel hybrid cements,significantly enhancing in vitro osteogenesis of bone marrow mesenchymal stem cells by up-regulating the gene expression of Runx2,Coll,and OPN.Use of PMMA-hydrogel hybrid cements loaded with BMP-2 on rabbit femoral condyle bone-defect models revealed their biodegradability and enhanced bone formation.Our study demonstrated the favorable mechanical properties,biocompatibility,and biodegradability of fabricated PMMA-hydrogel hybrid cements loaded with BMP-2,as well as their ability to improve osteogenesis,making them a promising material for use in PKP and PVP.展开更多
基金funded by the National Natural Science Foundation of China(32271379)Fundamental Research Funds for the Central Universities(YG2021ZD12).
文摘Objective:This study aims to investigate the regulatory effects of piezoelectricity on the inflammatory microenvironment in osteoporosis treatment.Impact Statement:Recent studies have extensively explored the impact of piezoelectric materials on macrophage polarization and cytokine secretion.However,the effects of piezoelectricity on macrophages for the regulation of immune osteogenesis in osteoporosis remain poorly understood.This study provides novel insights into the regulatory role of piezoelectricity in macrophage modulation within osteoporotic diseases.Introduction:The overexpression of various inflammatory factors in osteoporosis exacerbates bone metabolism imbalance.Macrophage polarization plays a pivotal role in inflammation regulation and tissue repair.Therefore,investigating the regulatory effects of piezoelectricity on macrophage polarization is crucial for improving the inflammatory microenvironment and fostering an immune environment conducive to osteoporotic bone regeneration.Methods:This study fabricated polarized potassium sodium niobate ceramic(PKNN)piezoelectric bioceramics using solid-phase sintering and high-pressure polarization techniques,and investigated their regulatory effects on macrophage polarization,anti-inflammatory factor expression,and osteogenic differentiation bone marrow mesenchymal stem cells derived from ovariectomized rats(rBMSCs-OVX).Results:PKNN substantially promotes M2 macrophage polarization and enhances anti-inflammatory factor expression,effectively suppressing inflammatory responses.Further studies demonstrate that PKNN,by modulating macrophages,indirectly regulates osteoblast gene expression,improving the inhibitory effects of the pathological microenvironment on osteogenic differentiation of rBMSCs-OVX.Conclusion:This research provides important theoretical evidence for the development of immunomodulatory osteoporotic bone regeneration materials driven by piezoelectricity.
基金supported by the“Pioneer”and“Leading Goose”R&D Program of Zhejiang(2024C03080)the National Natural Science Foundation of China(52450110,52501320,and 52401310)the Science and Technology Commission of Shanghai Municipality(24ZR1475600).
文摘Objective:This work aims to construct a functional titanium surface with spontaneous electrical stimulation for immune osteogenesis and antibacteria.Impact Statement:A silver–calcium microgalvanic cell was engineered on the titanium implant surface to spontaneously generate microcurrents for osteoimmunomodulation and bacteria killing,which provides a promising strategy for the design of a multifunctional electroactive titanium implant.Introduction:Titanium-based implants are usually bioinert,which often leads to inflammation-induced loosening.Electrical stimulation has therapeutic potential;however,its dependence on external devices limits its clinical application.Therefore,designing an electroactive titanium surface with endogenous electrical stimulation capability is a promising strategy to overcome implant failure induced by inflammation.Methods:The silver–calcium micro-galvanic cell was constructed on titanium substrate surfaces by the ion implantation technique.RAW264.7 and MC3T3-E1 were used for cell culture studies with the material to evaluate immunomodulatory and osteogenic abilities of the implant.The expression levels of inflammatory genes and voltage-gated Ca2+channel-related genes were tested for investigating the mechanism of immunoregulation.The antibacterial properties of the modified titanium were assessed.Finally,its immunomodulatory effects in vivo were verified by a mouse subcutaneous inflammation model.Results:The silver–calcium micro-galvanic modified titanium surface generates microcurrents and releases Ca^(2+),which induces macrophage polarization toward the M2 phenotype and promotes osteogenic differentiation via paracrine signaling,exhibiting excellent antibacterial activity.Conclusion:The silver–calcium micro-galvanic cell on titanium could regulate the immune response to promote bone repair and exhibit antibacterial capabilities through noninvasive electrical stimulation,providing a promising strategy for the design of multifunctional electroactive implant surfaces.
基金supported by grants from the National Natural Science Foundation of China(Nos.31971106,BWS21L013,and 21WS09002).
文摘Bone defects have serious economic and clinical impacts;however,despite improvements in bone defect management,the range of clinical outcomes remains limited.A variety of biomaterials have been used to treat complex bone defects.However,final bone repair outcomes may be adversely affected by poor osteogenic capacity and risk of infection.Consequently,therapeutic methods are required that reduce bacterial contamination and increase the use of osteogenic biomaterials.Herein,we report the preparation of poly(lactic acid-coglycolic acid)(PLGA)microspheres coloaded with magnesium(Mg^(2+))and gallium(Ga^(3+))ions(Mg-Ga@PLGA),which can fill irregular bone defects and show good biosafety.During in vitro testing,Mg-Ga@PLGA not only showed a synergistic effect on promoting osteogenic differentiation but also inhibited osteoclastic differentiation.Moreover,we found that Mg-Ga@PLGA demonstrated an antibacterial effect.During in vivo testing,Mg Ga@PLGA exhibited strong in situ osteogenic ability.In conclusion,Mg-Ga@PLGA has good potential for treating bone defects at risk of infection.
基金supported by central government-guided major science and technology project of Hebei province 236Z7709G(M.C.Q.)Tangshan science and technology project 23130216E(M.C.Q.)+8 种基金key research projects of North China University of Science and Technology ZD-YG-202309(M.C.Q.)National Natural Science Foundations of China 82230030 and 81871492(Y.L.)Beijing International Science and Technology Cooperation Project Z221100002722003(Y.L.)Beijing Natural Science Foundation L234017(Y.L.)Peking University Medicine plus X Pilot Program-Key Technologies R&D Project 2024YXXLHGG004(Y.L.)Key R&D Plan of Ningxia Hui Autonomous Region 2020BCG01001(Y.L.)First-Class Discipline Team of Kunming Medical University 2024XKTDTS08(Y.L.)Innovative Research Team of High-level Local Universities in Shanghai SHSMU-ZLCX20212402(Y.L.)Postdoctoral Fellowship Program of CPSF under Grant Number GZB20240038(X.J.C.).
文摘Osteogenesis is the process of bone formation mediated by the osteoblasts,participating in various bone-related physiological processes including bone development,bone homeostasis and fracture healing.It exhibits temporal and spatial interconnectivity with angiogenesis,constructed by multiple forms of cell communication occurring between bone and vascular endothelial cells.Molecular regulation among different cell types is crucial for coordinating osteogenesis and angiogenesis to facilitate bone remodeling,fracture healing,and other bone-related processes.The transmission of signaling molecules and the activation of their corresponding signal pathways are indispensable for various forms of cell communication.This communication acts as a“bridge”in coupling osteogenesis to angiogenesis.This article reviews the modes and processes of cell communication in osteogenesisangiogenesis coupling over the past decade,mainly focusing on interactions among bone-related cells and vascular endothelial cells to provide insights into the mechanism of cell communication of osteogenesis-angiogenesis coupling in different bone-related contexts.Moreover,clinical relevance and applications are also introduced in this review.
基金supported by the National Key Research and Development Program of China(2021YFC2400700)the National Science Foundation of China(82370924,82170929)+5 种基金the Beijing Natural Science Foundation Haidian Original Innovation Joint Fund Project(L222090)the Fujian Province Natural Science Foundation of China(2021J01803)the Postdoctoral Fellowship Program of 5CPSF(GZC20230141)the Beijing Municipal Natural Science Foundation-Changping Innovation Joint Fund Project(L234074)Industry-University-Research Innovation Fund for Chinese Universities(2024GR021)the Innovation research program(HHKT-00-03).
文摘Guided bone regeneration(GBR)membranes are extensively utilized in dental implantation.However,the existing GBR membranes showed insufficient space-maintaining capability and poor bone promoting ability,affecting the effectiveness of clinical bone augmentation,which in turn resulted in poor implant outcomes and even failure.In this study,we designed a novel magnesium reinforced sandwich structured composite membrane,consisting of an inner magnesium scaffold and a PLGA/collagen hybrid(mixture of poly(lactic-co-glycolic acid)and collagen)top and bottom layer.The magnesium scaffold provided mechanical support and released Mg^(2+)to enhance osteogenesis.The PLGA/collagen hybrid regulated membrane degradation and improved biocompatibility,promoting cell adhesion and proliferation(P<0.05).The PLGA/collagen hybrid regulated the release of magnesium ions,such that the MgP10C(mass ratios of PLGA and collagen=100:10)group showed the best in vitro osteogenic effect.Further mechanism exploration confirmed that MgP10C membranes significantly enhanced bone defect repair via the MAPK/ERK 1/2 pathway by the Mg^(2+)released from the composite membranes.In rat calvarial defect and rabbit alveolar defect model(P<0.05),the in vivo osteogenic effect of the MgP10C group was superior to that of other groups.Finite element analysis models validated the support effect of composite membranes,demonstrating lower stress and a significant reduction in strain on the bone graft in the MgP10C group.In conclusion,the magnesium-reinforced sandwich structure composite membrane,with its spacemaintaining properties and osteoinductive activity,represents a new strategy for GBR and enhancing osteogenic potential that meets directly clinical needs.
基金Supported by the National and Zhejiang Provincial Administration of Traditional Chinese Medicine Co-construction Project,No.GZYZJ-KJ-24080the Medical Science and Technology Project of Zhejiang Province,No.2024XY228.
文摘BACKGROUND Metabolic-associated fatty liver disease(MAFLD)is characterized by lipid accumulation in hepatocytes and is closely associated with oxidative stress.Increasing clinical evidence indicates that MAFLD is linked to bone metabolic disorders,including osteoporosis.Recent studies indicate that the expression profiles of liver circular RNAs(circRNAs)are altered in MAFLD.However,the effects of these changes on bone metabolism remain poorly understood.AIM To investigate the effects and mechanism of differently expressed circRNAs secreted by the liver on osteogenic differentiation in MAFLD.METHODS RNA sequencing was performed to identify highly expressed circRNAs in the liver,validated by quantitative real-time reverse transcription polymerase chain reaction,and localized using fluorescence in situ hybridization(FISH).A mouse model induced by a high-fat diet was used to simulate MAFLD.RESULTS CircSOD2 was significantly upregulated in liver tissues and primary hepatocytes from subjects with MAFLD.CircSOD2 was induced by oxidative stress and attenuated by antioxidants in the mouse model.In addition,circSOD2 was delivered from hepatocytes to bone marrow mesenchymal stem cells(BMSCs).Furthermore,circSOD2 inhibited the osteogenic differentiation of BMSCs and in vivo bone formation by sponging miR-29b.Moreover,miR-29b inhibition reversed the stimulatory effect of circSOD2 silencing on osteogenic differentiation of BMSCs and in vivo bone formation.Mechanistically,the interaction between circSOD2 and miR-29b confirmed through a luciferase reporter assay and the co-localization in the cytoplasm evidenced by FISH indicated that circSOD2 acted as a sponge for miR-29b.CONCLUSION This study provides a novel mechanism underlying the liver-bone crosstalk,demonstrating that circSOD2 upregulation in hepatocytes,induced by oxidative stress,inhibits osteogenic differentiation of BMSCs by sponging miR-29b.These findings offer a better understanding of the relationship between MAFLD and osteoporosis.
基金financially supported by the Natural Science Foundation of Guangdong Province(No.2022A1515010307)the Science and Technology Planning Project of Guangzhou(Nos.2023A03J0693 and 2023A03J0040)
文摘Steroid-induced osteonecrosis of the femoral head (SONFH) is a severe bone disorder that was clinically treated by core decompression (CD) combined with bone grafting.However,the clinical outcome of this therapy for SONFH is usually unsatisfactory.Magnesium (Mg) is a biodegradable material reported as an ideal orthopedic implant.The Mg^(2+)released from its degradation is considered an effective factor for bone tissue formation,but the rapid degradation rate limited its application.Here,we reported a magnesiumDscandium (MgDSc) alloy for promoting bone regeneration in SONFH.We discovered that MgDSc alloy with satisfied corrosion resistance,antiapoptosis,angiogenic,and osteogenic properties in vitro,could facilitate osteogenic differentiation by activating the Wnt/β-catenin signaling pathway.In the clinically relevant SONFH model,CD combined with suitable MgDSc alloy implantation promoted angiogenesisDosteogenesis coupling and alleviated osteonecrosis.We observed fewer apoptotic osteocytes and empty bone lacunae,but more blood vessels and new bone formation in the Mg-Sc alloy implanted CD tunnel region compared with CD-treated SONFH rabbits.Properly degraded MgDSc alloy maintains high structural integrity and provides reliable mechanical support for the osteonecrotic femoral head,which is conducive to further clinical translation.
基金supported by the National Key Research and Development Program of China(2023YFB3813000)the National Natural Science Foundation of China(32225028 and 32130062).
文摘Objective:This study aims to clarify the effects of bioceramic interface cues on macrophages.Impact Statement:Recently,there have been many researches exploring the effects of interface topography cues on macrophage polarization and cytokine secretion.However,the effects and underlying mechanisms of bioceramic interface cues on macrophages still need exploring.This study provides insights into the effects of bioceramic micro-groove surface structures on macrophages.Introduction:With the development of bone tissue engineering methods,bioceramics have been used for bone repair.After the implantation of bioceramics,innate immune response that occurs at the interface of materials can deeply influence the subsequent inflammation and bone regeneration progress.Therefore,the exploration and regulation of immune response of the bioceramic interface will be beneficial to promote the bone regeneration effects.Methods:In this study,bioceramics with micro-groove structures on the surface are fabricated by digital light processing 3-dimensional printing technology.Then,micro-groove structures with different spacings(0,25,50,and 75μm)are prepared separately to explore the effects on macrophages.Results:The large spacing micro-groove structure can promote the M2 polarization and osteoinductive cytokine secretion of macrophage.The reason is that the large spacing micro-groove structure can induce directional arrangement of macrophage so as to change the phenotype and cytokine secretion.Further researches show that macrophage of the large spacing micro-groove structure can promote the osteogenic differentiation of bone mesenchymal stem cells,which can benefit osteogenesis and osteointegration.Conclusion:This study offers an effective and application potential method for bone repair.
基金supported by the National Natural Science Foundation of China(51972339,52072023,and 51802350)the National Natural Science Found for Distinguished Young Scholars(52225101)+3 种基金the China Postdoctoral Science Foundation(2022M720551)the Natural Science Foundation of Chongqing(CSTB2023NSCQ-MSX0527,cstc2021jcyj-msxmX0993)the Chongqing Academician Special Fund(2022YSZX-JCX0014CSTB)the Chongqing Science and Technology Commission(CSTB2022NSCQ-MSX0416).
文摘Magnesium(Mg)alloys with biodegradability and excellent mechanical properties are in high demand for applications in guided bone regeneration(GBR).However,the clinical application of Mg alloys is hindered by infection risks and limited osteogenesis.Herein,a structure-functional integrated Mg-Ca/Mg-Cu bilayer membrane was rolled at 150℃through various single-pass reductions by using online heating rolling.The Mg-Cu layer was specifically engineered to exhibit antibacterial properties tailored for gingival tissue,while the Mg-Ca layer was designed to support bone regeneration within the defect cavity.The bilayer membrane demonstrated a flexural yield strength of 421.0 MPa and a modulus of 58.6 GPa,indicating exceptional deformation resistance.Furthermore,it maintained notable structural stability by retaining 86.4%of its volume after 21 days in Hanks'solution.In vitro results revealed that the bilayer membrane exhibited favorable biocompatibility and promoted osteogenesis via the synergetic effect of released Mg^(2+)and Ca^(2+)ions.The rapid release of Cu^(2+)ions and the creation of an alkaline environment further improved antibacterial properties,potentially preventing postoperative infections.Additionally,in an in vivo rat calvarial defect model,the membrane demonstrated its capability to stimulate new bone formation.In summary,the Mg-Ca/Mg-Cu bilayer membrane exhibited outstanding mechanical stability,favorable corrosion rates,extraordinary osteogenic and antibacterial activity simultaneously.Consequently,it holds promise as a robust barrier membrane in GBR applications.
基金supported by the National Natural Science Foundation of China(Nos.52072127,52201297,U21A2055,and U22A20160)the China Postdoctoral Science Foundation(No.2022M711200)the Royal Society(No.IEC/NSFC/191344)(UK).
文摘Mimicking the electric microenvironment of natural tissue is a promising strategy for developing biomedical implants. However, current research has not taken biomimetic electrical functional units into consideration when designing biomedical implants. In this research, ordered structures with Schottky heterojunction functional unit (OSSH) were constructed on titanium implant surfaces for bone regeneration regulation. The Schottky heterojunction functional unit is composed of periodically distributed titanium microdomain and titanium oxide microdomain with different carrier densities and surface potentials. The OSSH regulates the M2-type polarization of macrophages to a regenerative immune response by activating the PI3K-AKT-mTOR signal pathway and further promotes osteogenic differentiation of rat bone marrow mesenchymal stem cells. This work provides fundamental insights into the biological effects driven by the Schottky heterojunction functional units that can electrically modulate osteogenesis.
基金supported by the National Natural Science Foundation of China(No.81901430)the Guangdong Basic and Applied Basic Research Foundation(No.2022A1515010379)+1 种基金the Science and Technology Projects in Guangzhou(No.2023A04J0555)the Guangdong Provincial Key Laboratory of Physical Activity and Health Promotion(No.2021B1212040014),China.
文摘Bone is a highly calcified and vascularized tissue.The vascular system plays a vital role in supporting bone growth and repair,such as the provision of nutrients,growth factors,and metabolic waste transfer.Moreover,the additional functions of the bone vasculature,such as the secretion of various factors and the regulation of bone-related signaling pathways,are essential for maintaining bone health.In the bone microenvironment,bone tissue cells play a critical role in regulating angiogenesis,including osteoblasts,bone marrow mesenchymal stem cells(BMSCs),and osteoclasts.Osteogenesis and bone angiogenesis are closely linked.The decrease in osteogenesis and bone angiogenesis caused by aging leads to osteoporosis.Long noncoding RNAs(lncRNAs)are involved in various physiological processes,including osteogenesis and angiogenesis.Recent studies have shown that lncRNAs could mediate the crosstalk between angiogenesis and osteogenesis.However,the mechanism by which lncRNAs regulate angiogenesis-osteogenesis crosstalk remains unclear.In this review,we describe in detail the ways in which lncRNAs regulate the crosstalk between osteogenesis and angiogenesis to promote bone health,aiming to provide new directions for the study of the mechanism by which lncRNAs regulate bone metabolism.
基金support from the National Institute of Health(K99AR081897,R00AR081897)M.N.W.acknowledges funding support from the National Institute of Health(P01DK011794,R01DK116716)+1 种基金the Smith Family Foundation Odyssey Award,and the Chen Institute Massachusetts General Hospital Research Scholar(2024-2029)awardμCT and bone histomorphometry were performed by the Center for Skeletal Research at Massachusetts General Hospital,a NIH-funded program(P30AR066261 and AR075042)led by Mary Bouxsein and Marie Demay.
文摘Osteogenesis imperfecta(OI)is a group of diseases caused by defects in type I collagen processing which result in skeletal fragility.While these disorders have been regarded as defects in osteoblast function,the role of matrix-embedded osteocytes in OI pathogenesis remains largely unknown.Homozygous human SP7(c.946 C>T,R316C)mutation results in a recessive form of OI characterized by fragility fractures,low bone mineral density and osteocyte dendrite defects.To better understand how the OI-causing R316C mutation affects the function of SP7,we generated Sp7^(R342C)knock-in mice.Consistent with patient phenotypes,Sp7^(R342C/R342C)mice demonstrate increased cortical porosity and reduced cortical bone mineral density.Sp7^(R342C/R342C)mice show osteocyte dendrite defects,increased osteocyte apoptosis,and intracortical bone remodeling with ectopic intracortical osteoclasts and elevated osteocyte Tnfsf11 expression.
基金supported by the National Natural Science Foundation of China(NSFC)(82371003)the Elite Research Grant of the International Orthodontics Foundation 2022。
文摘Insufficient alveolar bone thickness increases the risk of periodontal dehiscence and fenestration,especially in orthodontic tooth movement.Abaloparatide(ABL),a synthetic analog of human PTHr P(1–34)and a clinical medication for treating osteoporosis,has recently demonstrated its potential in enhancing craniofacial bone formation.Herein,we show that intraoral submucosal injection of ABL,when combined with mechanical force,promotes in situ alveolar bone thickening.The newly formed bone is primarily located outside the original compact bone,implying its origin from the periosteum.RNA sequencing of the alveolar bone tissue revealed that the focal adhesion(FA)pathway potentially mediates this bioprocess.Local injection of ABL alone enhances cell proliferation,collagen synthesis,and phosphorylation of focal adhesion kinase(FAK)in the alveolar periosteum;when ABL is combined with mechanical force,the FAK expression is upregulated,in line with the accomplishment of the ossification.In vitro,ABL enhances proliferation,migration,and FAK phosphorylation in periosteal stem cells.Furthermore,the pro-osteogenic effects of ABL on alveolar bone are entirely blocked when FAK activity is inhibited by a specific inhibitor.In summary,abaloparatide combined with mechanical force promotes alveolar bone formation via FAK-mediated periosteal osteogenesis.Thus,we have introduced a promising therapeutic approach for drug-induced in situ alveolar bone augmentation,which may prevent or repair the detrimental periodontal dehiscence,holding significant potential in dentistry.
基金supported by grants from the National Nature Science Foundation of China (No. 30772454)Science and Technology Bureau of Sichuan Province (No. 2006z09-013)
文摘Aim Understanding the response of mesenchymal stem cells （MSCs） to mechanical strain and their consequent gene expression patterns will broaden our knowledge of the mechanobiology of distraction osteogenesis. Methodology In this study, a single period of cyclic mechanical stretch （0.5 Hz, 2,000 με） was performed on rat bone marrow MSCs. Cellular proliferation and alkaline phosphatase （ALP） activity was examined. The mRNA expression of six bone-related genes （Ets-1, bFGF, IGF-Ⅱ, TGF-β, Cbfal and ALP） was detected using real-time quantitative RT-PCR. Results The results showed that mechanical strain can promote MSCs proliferation, increase ALP activity, and up-regulate the expression of these genes. A significant increase in Ets-1 expression was detected immediately after mechanical stimulation, but Cbfal expression became elevated later. The temporal expression pattem of ALP coincided perfectly with Cbfal. Conclusion The results of this study suggest that mechanical strain may act as a stimulator to induce differentiation of MSCs into osteoblasts, and that these bone-related genes may play different roles in the response of MSCs to mechanical stimulation.
基金financially supported by National Natural Science Foundation of China (81100240)‘985’ project of Sun Yat-Sen University grant+2 种基金Sun Yat-Sen university young teachers training project (13YKPY42)Natural Science Foundation of Guangdong Province,China(S2012010009495)Science and Technology Planning Project of Guangdong Province,China(2012B031800185)
文摘Age related defect of the osteogenic differentiation of mesenchymal stem cells（MSCs） plays a key role in osteoporosis. Mechanical loading is one of the most important physical stimuli for osteoblast differentiation.Here, we compared the osteogenic potential of MSCs from young and adult rats under three rounds of 2 h of cyclic stretch of 2.5% elongation at 1 Hz on 3 consecutive days. Cyclic stretch induced a significant osteogenic differentiation of MSCs from young rats, while a compromised osteogenesis in MSCs from the adult rats.Accordingly, there were much more reactive oxygen species（ROS） production in adult MSCs under cyclic stretch compared to young MSCs. Moreover, ROS scavenger N-acetylcysteine rescued the osteogenic differentiation of adult MSCs under cyclic stretch. Gene expression analysis revealed that superoxide dismutase 1（SOD1） was significantly downregulated in those MSCs from adult rats. In summary, our data suggest that reduced SOD1 may result in excessive ROS production in adult MSCs under cyclic stretch, and thus manipulation of the MSCs from the adult donors with antioxidant would improve their osteogenic ability.
基金Project (No. 20070421123) supported by the Postdoctoral Science Foundation of China
文摘Objective： We investigated the effects of intermittent negative pressure on osteogenesis in human bone marrowderived stroma cells （BMSCs） in vitro. Methods： BMSCs were isolated from adult marrow donated by a hip osteoarthritis patient with prosthetic replacement and cultured in vitro. The third passage cells were divided into negative pressure treatment group and control group. The treatment group was induced by negative pressure intermittently （pressure： 50 kPa, 30 min/times, and twice daily）. The control was cultured in conventional condition. The osteogenesis of BMSCs was examined by phase-contrast mi- croscopy, the determination of alkaline phosphatase （ALP） activities, and the immunohistochemistry of collagen type 1. The mRNA expressions of osteoprotegerin （OPG） and osteoprotegerin ligand （OPGL） in BMSCs were analyzed by real-time polymerase chain reaction （PCR）. Results： BMSCs showed a typical appearance of osteoblast after 2 weeks of induction by intermittent negative pressure, the activity of ALP increased significantly, and the expression of collagen type I was positive. In the treatment group, the mRNA expression of OPG increased significantly （P〈0.05） and the mRNA expression of OPGL decreased significantly （P〈0.05） after 2 weeks, compared with the control. Conclusion： Intermittent negative pressure could promote osteogenesis in human BMSCs in vitro.
基金supported in part by grants from the National Institutes of Health(AG051773)and VA(BX000838)
文摘YAP（yes-associated protein） is a transcriptional factor that is negatively regulated by Hippo pathway, a conserved pathway for the development and size control of multiple organs. The exact function of YAP in bone homeostasis remains controversial. Here we provide evidence for YAP＇s function in promoting osteogenesis, suppressing adipogenesis, and thus maintaining bone homeostasis.YAP is selectively expressed in osteoblast（OB）-lineage cells. Conditionally knocking out Yap in the OB lineage in mice reduces cell proliferation and OB differentiation and increases adipocyte formation, resulting in a trabecular bone loss. Mechanistically, YAP interacts with β-catenin and is necessary for maintenance of nuclear β-catenin level and Wnt/β-catenin signaling. Expression of β-catenin in YAP-deficient BMSCs（bone marrow stromal cells） diminishes the osteogenesis deficit. These results thus identify YAP-β-catenin as an important pathway for osteogenesis during adult bone remodeling and uncover a mechanism underlying YAP regulation of bone homeostasis.
基金fnancially supported by the National Key Research and Development Program of China(Nos.2017YFB0702600 and 2017YFB0702602)the National Natural Science Foundation of China(No.31771044)+1 种基金the Shanghai Committee of Science and Technology,China(Nos.20S31901200,18YF1426900 and 19JC1415500)the International Partnership Program of Chinese Academy of Sciences Grant(No.GJHZ1850)。
文摘Attempt of developing bio-safety and functional layered double hydroxides(LDHs)modifed plasma electrolytic oxidation(PEO)coatings,has become a hotspot in the protection of magnesium(Mg)based biomedical implants.In the present work,Mg-Fe LDH flms with different Fe contents were fabricated on PEO coating via a novel two-step method:frstly,the Fe OOH flms were prepared by immersing PEO sample in Fe^(2+)-containing solution,and then Mg-Fe LDH flms were formed by transforming the Fe OOH flms via a hydrothermal treatment in water.The highly-oriented LDH nano-sheets could enhance the anti-corrosion performance of PEO coating,which was proved by the results of electrochemical test,hydrogen evolution and corroded morphology.PEO/Mg-Fe LDH coatings showed a low hemolysis rate(less5%)than PEO coating.In addition,PEO/Mg-Fe LDH coatings were more favorable for cell adhesion and proliferation than PEO coating.Moreover,PEO/Mg-Fe LDH coatings showed good photothermal conversion property,which demonstrated the excellent rapid antibacterial effect under NIR light.In vitro culture of rat bone marrow stem cell(r BMSC)suggested that cells cultured in the extract of PEO/Mg-Fe LDH coatings had a better osteogenic activity.In vivo subcutaneous implantation test revealed that PEO/Mg-Fe LDH coatings exhibited good anti-corrosion and histocompatibility.
基金supported by National Natural Science Foundation of China (81600828)Shanghai Sailing Program (16YF1406600)
文摘Ginsenoside Rb1, the effective constituent of ginseng, has been demonstrated to play favorable roles in improving the immunity system. However, there is little study on the osteogenesis and angiogenesis effect of Ginsenoside Rb1. Moreover, how to establish a delivery system of Ginsenoside Rb1 and its repairment ability in bone defect remains elusive. In this study, the role of Ginsenoside Rb1 in cell viability, proliferation, apoptosis, osteogenic genes expression, ALP activity of rat BMSCs were evaluated firstly. Then,micro-nano HAp granules combined with silk were prepared to establish a delivery system of Ginsenoside Rb1, and the osteogenic and angiogenic effect of Ginsenoside Rb1 loaded on micro-nano HAp/silk in rat calvarial defect models were assessed by sequential fluorescence labeling, and histology analysis, respectively. It revealed that Ginsenoside Rb1 could maintain cell viability, significantly increased ALP activity, osteogenic and angiogenic genes expression. Meanwhile, micro-nano HAp granules combined with silk were fabricated smoothly and were a delivery carrier for Ginsenoside Rb1. Significantly, Ginsenoside Rb1 loaded on micro-nano HAp/silk could facilitate osteogenesis and angiogenesis. All the outcomes hint that Ginsenoside Rb1 could reinforce the osteogenesis differentiation and angiogenesis factor’s expression of BMSCs. Moreover, micro-nano HAp combined with silk could act as a carrier for Ginsenoside Rb1 to repair bone defect.
基金supported by the National Key R&D Program of China(No.2018YFA0703000)the National Natural Science Foundation of China(Nos.82071564,82072412,and 81772326)+1 种基金the Fundamental Research Program Funding of Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine(No.JYZZ070)Project of Shanghai Science and Technology Commission(No.19XD1434200/18431903700)。
文摘Poly methyl methacrylate(PMMA)bone cement is used in augmenting and stabilizing fractured vertebral bodies through percutaneous vertebroplasty(PVP)and percutaneous kyphoplasty(PKP).However,applications of PMMA bone cement are limited by the high elasticity modulus of PMMA,its low biodegradability,and its limited ability to regenerate bone.To improve PMMA bio activity and biodegradability and to modify its elasticity modulus,we mixed PMMA bone cement with oxidized hyaluronic acid and carboxymethyl chitosan in situ cross-linking hydrogel loaded with bone morphogenetic protein-2(BMP-2)to achieve novel hybrid cement.These fabric ated PMMA-hydrogel hybrid cements exhibited lower setting temperatures,a lower elasticity modulus,and better biodegradability and biocompatibility than that of pure PMMA cement,while retaining acceptable setting times,mechanical strength,and inj ectability.In addition,we detected release of BMP-2 from the PMMA-hydrogel hybrid cements,significantly enhancing in vitro osteogenesis of bone marrow mesenchymal stem cells by up-regulating the gene expression of Runx2,Coll,and OPN.Use of PMMA-hydrogel hybrid cements loaded with BMP-2 on rabbit femoral condyle bone-defect models revealed their biodegradability and enhanced bone formation.Our study demonstrated the favorable mechanical properties,biocompatibility,and biodegradability of fabricated PMMA-hydrogel hybrid cements loaded with BMP-2,as well as their ability to improve osteogenesis,making them a promising material for use in PKP and PVP.
