Nuclear factor Y(NF-Y),a group of conserved transcription-factor complexes that consist of NF-YA,B,and C subunits,is essential for developmental regulation and for responses to environmental changes in eukaryotes.We p...Nuclear factor Y(NF-Y),a group of conserved transcription-factor complexes that consist of NF-YA,B,and C subunits,is essential for developmental regulation and for responses to environmental changes in eukaryotes.We previously found that some NF-Y genes,such as OsNF-YA8,were expressed specifically in the endosperm of rice.In the present study,overexpression of OsNF-YA8 in rice resulted in reduced plant height due to suppressed cell elongation in internodes.Gibberellin(GA)biosynthetic genes,including OsCPS1,OsGA20ox1,and OsGA20ox2,were down-regulated.OsNF-YA8 bound to the promoters of these genes to repress their expression.Endogenous GA content was decreased in OsNF-YA8 overexpressors,whose dwarf phenotype could be partially rescued by exogenous GA treatment.The findings suggested that ectopic expression of OsNF-YA8 causes defective GA biosynthesis in vegetative stage.Heading date in OsNF-YA8 overexpressors was delayed,especially under short-day conditions.OsNFYA8 bound to the promoter of Heading Date 3a(Hd3a),the florigen gene in rice,to negatively regulate flowering.Either ectopic activation or knockout of OsNF-YA8 impaired seed development,as indicated by reduced seed size and increased grain chalkiness.These results suggest that ectopic expression of the endosperm-specific OsNF-YA8 in rice disrupts both vegetative and reproductive development.展开更多
Grain weight,a key determinant of yield in rice(Oryza sativa L.),is governed primarily by genetic factors,whereas grain chalkiness,a detriment to grain quality,is intertwined with environmental factors such as mineral...Grain weight,a key determinant of yield in rice(Oryza sativa L.),is governed primarily by genetic factors,whereas grain chalkiness,a detriment to grain quality,is intertwined with environmental factors such as mineral nutrients.Nitrogen(N)is recognized for its effect on grain chalkiness,but the underlying molecular mechanisms remain to be clarified.This study revealed the pivotal role of rice NODULE INCEPTION-LIKE PROTEIN 3(OsNLP3)in simultaneously regulating grain weight and grain chalkiness.Our investigation showed that loss of OsNLP3 leads to a reduction in both grain weight and dimension,in contrast to the enhancement observed with OsNLP3 overexpression.OsNLP3 directly suppresses the expression of OsCEP6.1 and OsNF-YA8,which were identified as negative regulators associated with grain weight.Consequently,two novel regulatory modules,OsNLP3-OsCEP6.1 and OsNLP3-OsNF-YA8,were identified as key players in grain weight regulation.Notably,the OsNLP3-OsNF-YA8 module not only increases grain weight but also mitigates grain chalkiness in response to N.This research clarifies the molecular mechanisms that orchestrate grain weight through the OsNLP3-OsCEP6.1 and OsNLP3-OsNF-YA8 modules,highlighting the pivotal role of the OsNLP3-OsNF-YA8 module in alleviating grain chalkiness.These findings reveal potential targets for simultaneous enhancement of rice yield and quality.展开更多
基金the National Natural Science Foundation of China(31701392 and 32170344)the Six Talent Peaks Project in Jiangsu Province(NY-142)+1 种基金the Jiangsu Province Government(JBGS[2021]001)the Independent Scientific Research Project Funds of the Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding(PLR202101).
文摘Nuclear factor Y(NF-Y),a group of conserved transcription-factor complexes that consist of NF-YA,B,and C subunits,is essential for developmental regulation and for responses to environmental changes in eukaryotes.We previously found that some NF-Y genes,such as OsNF-YA8,were expressed specifically in the endosperm of rice.In the present study,overexpression of OsNF-YA8 in rice resulted in reduced plant height due to suppressed cell elongation in internodes.Gibberellin(GA)biosynthetic genes,including OsCPS1,OsGA20ox1,and OsGA20ox2,were down-regulated.OsNF-YA8 bound to the promoters of these genes to repress their expression.Endogenous GA content was decreased in OsNF-YA8 overexpressors,whose dwarf phenotype could be partially rescued by exogenous GA treatment.The findings suggested that ectopic expression of OsNF-YA8 causes defective GA biosynthesis in vegetative stage.Heading date in OsNF-YA8 overexpressors was delayed,especially under short-day conditions.OsNFYA8 bound to the promoter of Heading Date 3a(Hd3a),the florigen gene in rice,to negatively regulate flowering.Either ectopic activation or knockout of OsNF-YA8 impaired seed development,as indicated by reduced seed size and increased grain chalkiness.These results suggest that ectopic expression of the endosperm-specific OsNF-YA8 in rice disrupts both vegetative and reproductive development.
基金supported by grants from the National Natural Science Foundation of China(32321001 to Z.Z.and C.-B.X.and 32100208 to J.W.)the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24010303)+1 种基金the Anhui Provincial Natural Science Foundation(2108085QC103)the Fundamental Research Funds for the Central Universities(WK9100000023).
文摘Grain weight,a key determinant of yield in rice(Oryza sativa L.),is governed primarily by genetic factors,whereas grain chalkiness,a detriment to grain quality,is intertwined with environmental factors such as mineral nutrients.Nitrogen(N)is recognized for its effect on grain chalkiness,but the underlying molecular mechanisms remain to be clarified.This study revealed the pivotal role of rice NODULE INCEPTION-LIKE PROTEIN 3(OsNLP3)in simultaneously regulating grain weight and grain chalkiness.Our investigation showed that loss of OsNLP3 leads to a reduction in both grain weight and dimension,in contrast to the enhancement observed with OsNLP3 overexpression.OsNLP3 directly suppresses the expression of OsCEP6.1 and OsNF-YA8,which were identified as negative regulators associated with grain weight.Consequently,two novel regulatory modules,OsNLP3-OsCEP6.1 and OsNLP3-OsNF-YA8,were identified as key players in grain weight regulation.Notably,the OsNLP3-OsNF-YA8 module not only increases grain weight but also mitigates grain chalkiness in response to N.This research clarifies the molecular mechanisms that orchestrate grain weight through the OsNLP3-OsCEP6.1 and OsNLP3-OsNF-YA8 modules,highlighting the pivotal role of the OsNLP3-OsNF-YA8 module in alleviating grain chalkiness.These findings reveal potential targets for simultaneous enhancement of rice yield and quality.
