Propolis is a natural resin used around the world to treatment of several diseases. We evaluated the effect of Brazilian propolis (P1) in guinea pig isolated trachea in vitro contracted with hista-mine, in paw oedema ...Propolis is a natural resin used around the world to treatment of several diseases. We evaluated the effect of Brazilian propolis (P1) in guinea pig isolated trachea in vitro contracted with hista-mine, in paw oedema induced by histamine in mice and anti-ulcerogenic effect of propolis formulation in mice. We used a P1 extract from South of Brazil to produce a formulation containing sodium bicarbonate and propolis dry extract (2%). In the guinea pig trachea Brazilian propolis extract (10 μg/mL) produced a non-significant inhibition in the cumulative response curve for histamine. However, the dose of 100 μg/mL shown had a significant inhibitory effect on the contraction induced by histamine (51% ± 5%). In the mouse paw oedema induced by intraplantar injection of histamine (50 μg/paw), the results showed that the treatment of the mice with P1 (10 or 100 mg/Kg (p.o) or 1 or 10 mg/Kg (i.p)), 0.5 h beforehand, significantly inhibited the paw oedema with maximal inhibition (MI) of 25% or 42% by p.o. route or 22% or 37% by i.p. route, respectively. Finally we have pre-treated mice with P1 formulation (20 mg/Kg or 40 mg/Kg, p.o, one time) or vehicle (powder) in animals submitted to diclofenac-induced ulcer, after 8 h P1 formulation inhibited of 43% or 83% of ulcers scores, respectively. Results showed that P1 formulation inhibited the ulcerogenic effect induced by diclofenac. Results suggested also, at least in part, the antiulcerogenic effect of formulation containing standardized extract of propolis can be due to the antagonistic action on the histaminergic system.展开更多
以乌鸡和天麻为原料,制备天麻提取液和乌鸡酶解液,两者科学配伍并添加蜂蜜、柠檬酸、蛋白糖辅料,研制了乌鸡天麻口服液。通过单因素试验及正交试验确定口服液的最佳配方:天麻提取液19 m L/100 m L、乌鸡水解液68 m L/100 m L、蜂蜜添加...以乌鸡和天麻为原料,制备天麻提取液和乌鸡酶解液,两者科学配伍并添加蜂蜜、柠檬酸、蛋白糖辅料,研制了乌鸡天麻口服液。通过单因素试验及正交试验确定口服液的最佳配方:天麻提取液19 m L/100 m L、乌鸡水解液68 m L/100 m L、蜂蜜添加量6%、柠檬酸添加量0.15%、蛋白糖添加量0.075%。乌鸡天麻口服液为澄清透明的棕褐色液体,具有天麻独特的风味和鸡肉的香味,酸甜适口,无异味,营养价值高,经80℃,10 min灭菌后,符合产品质量标准。展开更多
文摘Propolis is a natural resin used around the world to treatment of several diseases. We evaluated the effect of Brazilian propolis (P1) in guinea pig isolated trachea in vitro contracted with hista-mine, in paw oedema induced by histamine in mice and anti-ulcerogenic effect of propolis formulation in mice. We used a P1 extract from South of Brazil to produce a formulation containing sodium bicarbonate and propolis dry extract (2%). In the guinea pig trachea Brazilian propolis extract (10 μg/mL) produced a non-significant inhibition in the cumulative response curve for histamine. However, the dose of 100 μg/mL shown had a significant inhibitory effect on the contraction induced by histamine (51% ± 5%). In the mouse paw oedema induced by intraplantar injection of histamine (50 μg/paw), the results showed that the treatment of the mice with P1 (10 or 100 mg/Kg (p.o) or 1 or 10 mg/Kg (i.p)), 0.5 h beforehand, significantly inhibited the paw oedema with maximal inhibition (MI) of 25% or 42% by p.o. route or 22% or 37% by i.p. route, respectively. Finally we have pre-treated mice with P1 formulation (20 mg/Kg or 40 mg/Kg, p.o, one time) or vehicle (powder) in animals submitted to diclofenac-induced ulcer, after 8 h P1 formulation inhibited of 43% or 83% of ulcers scores, respectively. Results showed that P1 formulation inhibited the ulcerogenic effect induced by diclofenac. Results suggested also, at least in part, the antiulcerogenic effect of formulation containing standardized extract of propolis can be due to the antagonistic action on the histaminergic system.
文摘以乌鸡和天麻为原料,制备天麻提取液和乌鸡酶解液,两者科学配伍并添加蜂蜜、柠檬酸、蛋白糖辅料,研制了乌鸡天麻口服液。通过单因素试验及正交试验确定口服液的最佳配方:天麻提取液19 m L/100 m L、乌鸡水解液68 m L/100 m L、蜂蜜添加量6%、柠檬酸添加量0.15%、蛋白糖添加量0.075%。乌鸡天麻口服液为澄清透明的棕褐色液体,具有天麻独特的风味和鸡肉的香味,酸甜适口,无异味,营养价值高,经80℃,10 min灭菌后,符合产品质量标准。
