There are more than 2000 species of brittle stars in the world. For most of them, many scientific questions including basic characteristics of eco-physiology are still unknown. In the present study, Ophiopholis sarsii...There are more than 2000 species of brittle stars in the world. For most of them, many scientific questions including basic characteristics of eco-physiology are still unknown. In the present study, Ophiopholis sarsii vadicola acclimated at 15 ℃, salinity 31, were assessed for temperature monia excretion were studied at different temperatures （5, 10, and salinity tolerance. Its oxygen consumption and am- 15, 20, 25℃） and salinities （25, 30, 35）. O. sarsii vadi- cola could tolerate 0-24℃ and no brittle star was dead in the salinity range of 19-48 in the experimental situation. Two-way ANOVA showed that the oxygen consumption and ammonia excretion normalized with both dry mass and wet mass, Q10, which is used to describe the temperature sensitivity of respiration, and moisture content were significantly affected by temperature and salinity, and the combined effects of the two factors were significant. Stepwise multiple regression analysis revealed that logarithmic oxygen consumption and ammonia excretion showed a significant positive relationship with logarithmic temperature and salinity. The logarithmic moisture content of the brittle stars showed an inverse relationship with logarithmic salinity, but a positive relationship with logarithmic temperature. This suggests that the tolerance of temperature and salinity of brittle stars is closely related to their living environment, and that the effects of temperature on oxygen consumption are more significant at higher salinity, and that the ammonia excretion is less affected by salinity at lower temperatures.展开更多
Ophiopholis mirabilis is a common species with a high population density on the western coasts of the northern Pacifi c Ocean.The number of O.mirabilis has been increasing recently in the scallop aquaculture zone(the ...Ophiopholis mirabilis is a common species with a high population density on the western coasts of the northern Pacifi c Ocean.The number of O.mirabilis has been increasing recently in the scallop aquaculture zone(the Zhangzi Island area,northern Yellow Sea)in China.To explore the mechanism of its population variation,the reproductive cycle of O.mirabilis was investigated in this area(39°04′N;122°51′E)from February 2017 through January 2018 and determined by the monthly gonad index(GI),histological examinations of the gonads and the oocyte size-frequency distribution.O.mirabilis had a clear annual reproductive cycle that was synchronous between males and females.Sea temperature and food availability played important roles in O.mirabilis reproduction.The GI value was less reliable for determining reproductive activity in O.mirabilis because the nutritive tissues within the gonads may be utilized to synthesize gametes,leading to a decrease in GI during maturation.The histological results also show that abundant nutritive phagocytes were present in the gonads of O.mirabilis,which,together with the germ cells,aff ected the weight of the gonads.In addition,the mature oocytes of O.mirabilis were relatively small(75–150μm),indicating that the larval development was planktotrophic.This study provided insights into the reproductive patterns and biology of O.mirabilis and is an essential basis for the quantity control of this species in aquaculture areas.展开更多
基金supported by the National Basic Research Program of China (Grant No. 2011CB409805)the National Science & Technology Pillar Program (Grant No. 2011BAD13B06)+1 种基金Special Scientific Research Funds for Central Non-profit Institutes, Yellow Sea Fisheries Research Institute (20603022013042)the National Science and Technology Planning Project of China (Grant No. 2011BAD13B05)
文摘There are more than 2000 species of brittle stars in the world. For most of them, many scientific questions including basic characteristics of eco-physiology are still unknown. In the present study, Ophiopholis sarsii vadicola acclimated at 15 ℃, salinity 31, were assessed for temperature monia excretion were studied at different temperatures （5, 10, and salinity tolerance. Its oxygen consumption and am- 15, 20, 25℃） and salinities （25, 30, 35）. O. sarsii vadi- cola could tolerate 0-24℃ and no brittle star was dead in the salinity range of 19-48 in the experimental situation. Two-way ANOVA showed that the oxygen consumption and ammonia excretion normalized with both dry mass and wet mass, Q10, which is used to describe the temperature sensitivity of respiration, and moisture content were significantly affected by temperature and salinity, and the combined effects of the two factors were significant. Stepwise multiple regression analysis revealed that logarithmic oxygen consumption and ammonia excretion showed a significant positive relationship with logarithmic temperature and salinity. The logarithmic moisture content of the brittle stars showed an inverse relationship with logarithmic salinity, but a positive relationship with logarithmic temperature. This suggests that the tolerance of temperature and salinity of brittle stars is closely related to their living environment, and that the effects of temperature on oxygen consumption are more significant at higher salinity, and that the ammonia excretion is less affected by salinity at lower temperatures.
基金Supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Nos.XDA23050501,XDA19060201)the Key Program for International S&T Cooperation Projects:Sino-Australian Center for Healthy Coasts(No.2016YFE0101500)+1 种基金the Shandong Joint Fund for Marine Ecology and Environmental Sciences(No.U1606404)the Taishan Scholars Project to SUN Song。
文摘Ophiopholis mirabilis is a common species with a high population density on the western coasts of the northern Pacifi c Ocean.The number of O.mirabilis has been increasing recently in the scallop aquaculture zone(the Zhangzi Island area,northern Yellow Sea)in China.To explore the mechanism of its population variation,the reproductive cycle of O.mirabilis was investigated in this area(39°04′N;122°51′E)from February 2017 through January 2018 and determined by the monthly gonad index(GI),histological examinations of the gonads and the oocyte size-frequency distribution.O.mirabilis had a clear annual reproductive cycle that was synchronous between males and females.Sea temperature and food availability played important roles in O.mirabilis reproduction.The GI value was less reliable for determining reproductive activity in O.mirabilis because the nutritive tissues within the gonads may be utilized to synthesize gametes,leading to a decrease in GI during maturation.The histological results also show that abundant nutritive phagocytes were present in the gonads of O.mirabilis,which,together with the germ cells,aff ected the weight of the gonads.In addition,the mature oocytes of O.mirabilis were relatively small(75–150μm),indicating that the larval development was planktotrophic.This study provided insights into the reproductive patterns and biology of O.mirabilis and is an essential basis for the quantity control of this species in aquaculture areas.
