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Realizing profits immediately:disposition effect of open-end fund investors in China 被引量:1
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作者 Li Yajing1 Zhu Hongquan2 Luan Mingjing2(1 School of Economics and Management, University of Electronic Science and Technology of China,Chengdu 610054, China)(2 School of Economics and Management, Southwest Jiaotong University, Chengdu 610031, China) 《Journal of Southeast University(English Edition)》 EI CAS 2008年第S1期180-186,共7页
This paper explores the disposition effect, the tendency of investors to hold losing investments too long and sell winning investments too soon, by analyzing a unique data set that consists of the buying and selling p... This paper explores the disposition effect, the tendency of investors to hold losing investments too long and sell winning investments too soon, by analyzing a unique data set that consists of the buying and selling positions of open-end fund investors in China. Results show that investors' buying and selling behaviors are significantly affected by fund performance. The reasons that lie behind such results are mainly related to the non-persistence of fund performance. When fund performance is non-persistent, investors are not clear about the future returns on funds. So, investors, especially individual investors, would have to realize the profits on hand immediately. Meanwhile, fund investors are highly sensitive to fund age and loading fees, but not sensitive to management fees charged by funds when they buy or sell fund shares. 展开更多
关键词 INVESTORS open-end fund disposition effect fund shares
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Effect of joints on microwave fracturing of the Bukit Timah granite using an open-ended antenna 被引量:2
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作者 Zhongjun Ma Yanlong Zheng +1 位作者 Lei He Jianchun Li 《Deep Underground Science and Engineering》 2022年第2期138-147,共10页
Microwave fracturing of rocks before mechanical breakage could improve the performance of mechanical excavators and reduce environmental impacts.Previous research focused on the microwave fracturing of intact rock blo... Microwave fracturing of rocks before mechanical breakage could improve the performance of mechanical excavators and reduce environmental impacts.Previous research focused on the microwave fracturing of intact rock blocks.By using an open-ended antenna,this paper investigates the effect of pre-existing joints on the microwave fracturing of the Singapore Bukit Timah granite blocks.The results show that the specimens are weakened in the manners of cracking,spalling,melting,or a combination of them.The crack number and the total crack length produced by microwave treatment of jointed rock blocks are slightly smaller than those in the intact rock blocks.The interaction between joints and microwave-induced cracks can be summarized into the following four patterns:(1)microwave-induced cracks become arrested so that the crack propagation is terminated;(2)microwave-induced cracks penetrate the joints and continue to propagate;(3)microwave-induced cracks become deflected along the joints;and(4)microwave-induced cracks propagate forward following the joints.The smaller the approach angle between the microwave-induced crack and the preexisting joint is,the more microwave-induced cracks tend to be arrested at the joint.Increasing the approach angle between the microwave-induced crack and the joint can increase the chance of microwave-induced crack penetrating the joint.The results also show that the smaller the distance is between the microwave radiation point and the joint,the easier it is for microwave-induced cracks to penetrate the joints;otherwise,the microwave-induced crack is more likely to be arrested at the pre-existing joint. 展开更多
关键词 Bukit Timah granite crack propagation JOINTS microwave treatment open-ended antenna
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Study on the squeezing effect of open-ended pipe piles
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作者 Yan-er Lu1,Jun-jie Zheng1,Jian-hua Yin21.Institute of Geotechnical and Underground Engineering,Huazhong University of Science and Technology,Wuhan 430074,China 2.Department of Civil and Structural Engineering,the Hong Kong Polytechnic University,Hong Kong,China. 《Journal of Pharmaceutical Analysis》 SCIE CAS 2009年第3期155-159,171,共6页
During the installation of a pipe pile,the soil around the pile will be squeezed out. This paper deals with this squeezing effect of open-ended pipe piles using the cylindrical cavity expansion theory. The characteris... During the installation of a pipe pile,the soil around the pile will be squeezed out. This paper deals with this squeezing effect of open-ended pipe piles using the cylindrical cavity expansion theory. The characteristics of soil with different tension and compression moduli and dilation are involved by applying the elastic theory with different moduli and logarithmic strain. The closed-form solutions of the radius of the plastic region,the displacement of the boundary between the plastic region and the elastic region and the expansion pressure on the external surface of the pipe piles are obtained. When obtaining these solutions,the soil plug in the open-ended pipe pile is considered by employing an incremental filling ratio to quantify the degree of soil plugging. Moreover,the effects of the ratio of tension and compression moduli,angle of dilation and incremental filling ratio on the radius of the plastic region and the expansion pressure on the external surface of the pipe pile are investigated. The parametric analyses show that it is necessary and important to consider the difference between the tension modulus and compression modulus,dilation angle and incremental filling ratio for studying the squeezing effect of open-ended pipe pile installation. It is concluded that the analytical solutions presented in this paper are suitable for studying the squeezing effect of open-ended pipe piles. 展开更多
关键词 open-ended pipe pile squeezing effect ratio of tension and compression moduli incremental filling ratio
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Zero-Sequence Current Suppression Strategy for Open-End Winding Permanent Magnet Synchronous Motor Based on Model Predictive Control
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作者 LIU Huashan LI Jie YAO Fei 《Journal of Donghua University(English Edition)》 EI CAS 2020年第4期286-292,共7页
Compared with the traditional three-phase star connection winding,the open-end winding permanent magnet synchronous motor(OW-PMSM)system with a common direct current(DC)bus has a zero-sequence circuit,which makes the ... Compared with the traditional three-phase star connection winding,the open-end winding permanent magnet synchronous motor(OW-PMSM)system with a common direct current(DC)bus has a zero-sequence circuit,which makes the common-mode voltage and the back electromotive force(EMF)harmonic generated by the inverters produce the zero-sequence current in the zero-sequence circuit,and the zero-sequence current has great influence on the operation efficiency and stability of the motor control system.A zero-sequence current suppression strategy is presented based on model predictive current control for OW-PMSM.Through the mathematical model of OW-PMSM to establish the predictive model and the zero-sequence circuit model,the common-mode voltage under different voltage vector combinations is fully considered during vector selection and action time calculation.Then zero-sequence loop constraints are established,so as to suppress the zero-sequence current.In the end,the control strategy proposed in this paper is verified by simulation experiments. 展开更多
关键词 open-end winding permanent magnet synchronous motor(OW-PMSM) zero-sequence current HARMONIC model predictive current control common-mode voltage
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Reform and Practice of an Engineering Postgraduate Training Pattern in an Open-Ended Base:Taking Foshan Base as an Example
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作者 Xinyu Huang Yajie Hu 《Journal of Contemporary Educational Research》 2022年第9期46-52,共7页
The flawed engineering practice is considered the main factor that is affecting to the development quality of engineering postgraduates.Taking Foshan Base as an example,this paper has analyzed the operational pattern,... The flawed engineering practice is considered the main factor that is affecting to the development quality of engineering postgraduates.Taking Foshan Base as an example,this paper has analyzed the operational pattern,practice teaching model,and internal governance system of the open-ended base as a new system for engineering practice and proposed several suggestions for the reformation of engineering postgraduates based on the construction effect. 展开更多
关键词 open-ended base Engineering postgraduate Foshan Base
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培养批判性思维的开放式功能学实验教学设计与实践
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作者 冯雪梅 陆琴 +2 位作者 刘燕 倪雯雯 许伟榕 《实验室研究与探索》 北大核心 2026年第1期168-173,共6页
人工智能(AI)技术的普及对学生批判性思维能力的培养构成了挑战,本研究基于“认知守财奴”理论与卡尼曼思考理论,提出一种开放式教学设计。该设计通过引入争议性质疑任务,阻断AI直接迁移可能;以强调证据的反思分析替代低阶作业,从而强... 人工智能(AI)技术的普及对学生批判性思维能力的培养构成了挑战,本研究基于“认知守财奴”理论与卡尼曼思考理论,提出一种开放式教学设计。该设计通过引入争议性质疑任务,阻断AI直接迁移可能;以强调证据的反思分析替代低阶作业,从而强制启动慢思考;并借助学术性激励机制建立动态反馈闭环,引导学生从直觉性“快思考”转向理性“慢思考”,最终实现“质疑-甄别-创新”的思维模式。研究表明,在开放式任务干预下,实验组在思维活跃度,质疑深度与创新表现方面均优于对照组,批判性思维测试结果进一步表明,实验组批判性思维总分明显高于对照组,尤其在“开放思想”和“分析力”两个维度上表现更为突出。这表明,开放式教学设计有助于将AI技术带来的潜在挑战,转化为促进学生高阶思维发展的契机,从而为AI时代医学教育模式创新提供理论与实践依据。 展开更多
关键词 开放式教学 批判性思维 认知守财奴理论 卡尼曼思考理论 医学功能学实验
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Design and analyses of open-ended pipe piles in cohesionless soils 被引量:2
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作者 Yuan GUO Xiong (Bill) YU 《Frontiers of Structural and Civil Engineering》 CSCD 2016年第1期22-29,共8页
Large open-ended pipe pile has been found to be advantageous for use in transportation projects. The current design method, however, is not adequately developed. To close this practice gap, this paper first summarized... Large open-ended pipe pile has been found to be advantageous for use in transportation projects. The current design method, however, is not adequately developed. To close this practice gap, this paper first summarized different design methods for open-ended pipe piles in sandy soils. A major factor for all the design codes is to properly account for the formation and effects of soil plug. The comparison indicates that there is a large variation in the base capacity evaluation among different methods due to the complex behaviors of soil plug. To close the knowledge gap, discrete element method (DEM) was used to simulate the soil plugging process and provide insight on the plugging mechanism. The simulation results show that the arching effect significantly increases the internal unit shear resistance along pipe piles. The porosity distribution and particle contact force distribution from DEM model indicate a large stress concentration occurs at the bottom of the soil plug. Besides, nearly 90% of the plug resistance is provided by the bottom half portion of the soil column. The soil-pile friction coefficient has a significant effect on the magnitude of plug resistance, with the major transition occurred for friction coefficient between 0.3 and 0.4. 展开更多
关键词 open-ended pipe pile soil plug DEM base capacity
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开放式问题自动评分中生成式人工智能幻觉抑制研究
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作者 张力跃 邓胜丹 《远程教育杂志》 北大核心 2026年第1期73-82,I0001,共11页
生成式人工智能在开放式问题自动评分中存在“幻觉”问题,常表现为事实性错误、逻辑矛盾、无关内容及过度自信表达,严重影响了教育评价的准确性与公平性。本研究基于深度学习与知识图谱理论,构建“数据—模型—应用”幻觉抑制框架:数据... 生成式人工智能在开放式问题自动评分中存在“幻觉”问题,常表现为事实性错误、逻辑矛盾、无关内容及过度自信表达,严重影响了教育评价的准确性与公平性。本研究基于深度学习与知识图谱理论,构建“数据—模型—应用”幻觉抑制框架:数据层建立基于学科、题型、评分等三维标注的专属结构化数据集,以融入评分逻辑;模型层设计包含评分维度拆解、学科知识校验、作答意图理解等模块的专用推理模型,并通过多任务联合损失函数进行优化;应用层搭建智能初评、风险分级、人机协同、标准更新的一体式闭环系统,实现常规案例自动处理与特殊案例精准干预。初步验证表明,该框架能够有效降低事实性幻觉发生率,提升评分的信效度与用户满意度,为教育评估智能化转型提供了可靠的技术路径参考。 展开更多
关键词 生成式人工智能 幻觉抑制 开放式问题 自动评分 事实性偏差
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A newly developed open-end intracranial hematoma drainage tube 被引量:1
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作者 Chao He Nongnaphat Wanussakul +2 位作者 Dong Yang Tianya Wu Ming Zhao 《Translational Neuroscience and Clinics》 2016年第2期150-152,共3页
Objective: To design a new open-end intracranial hematoma drainage tube for clinical application. Methods: The newly developed device consists of two parts: the plunger and barrel.On one side, the barrel is bullet sha... Objective: To design a new open-end intracranial hematoma drainage tube for clinical application. Methods: The newly developed device consists of two parts: the plunger and barrel.On one side, the barrel is bullet shaped with an opening tip. The plunger is located in the middle cavity of the tube barrel and extended out at the open-end. It was designed for strengthening the tube barrel and for convenience in performing the drainage procedure. It can be used by inserting the drainage tube into the lesion and pulling out the plunger, whereby blood will forcefully rise up inside the barrel, providing a satisfactory outcome. It is made for effusion drainage purposes. During the procedure, the drainage tip is placed at the deepest part of the intracranial hematoma to completely drain the blood. Moreover, the plunger fits tightly in the tube, preventing leakage during the operation. With the use of the device, brain can be separated. In addition, the device can help reduce the risk of cerebral damage because of the small operating area. The barrel sidewall has matching opening holes bilaterally and equally for exchanging substances between the inner and outer parts.The overlapping ratio in each horizontal pair is around 1/3–1/2. Each pair on the opposite side will form a different pressure. Thus, the opening holes will not easily get blocked with blood clot. Results: Blood and accumulated liquid from the deepest part of the intracranial hematoma can be directly drawn through the drainage tube without damaging a large area. The tube does not get blocked easily and allows for complete removal of the hematoma. Conclusions: The device is asuitable instrument for clinical application. 展开更多
关键词 open-end drainage tube BULLET shape barrel PLUNGER STEREOTAXIC drainage matching holes on each side
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Effects of inner sleeves on the inner frictional resistance of open-ended piles driven into sand
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作者 Janaka J. KUMARA Yoshiaki KIKUCHI +1 位作者 Takashi KURASHINA Takahiro YAJIMA 《Frontiers of Structural and Civil Engineering》 SCIE EI CSCD 2016年第4期499-505,共7页
In open-ended piles, inner friction is developed between inner pile shaf and the inner soil. Inner frictional resistance depends largely on the degree of soil plugging, which is influenced by many factors including pi... In open-ended piles, inner friction is developed between inner pile shaf and the inner soil. Inner frictional resistance depends largely on the degree of soil plugging, which is influenced by many factors including pile diameter, relative density and end conditions of piles. In this paper, effects of inner sleeves on inner frictional resistance are discussed. The experiments were conducted on a medium-dense sandy ground using laboratory-scale piles. It was observed that the piles penetrated under partially-plugged or unplugged state. The results suggest that inner fiictional resistance, Qin increases with sleeve height, l linearly and requires 2D (D is pile outer diameter) of l to produce a large as 50% of Qt by Qin (Qt is total resistance). The results also indicate that bearing capacity increases with wall thickness at the pile tip, which can be attributed to the increase in annular area. The results also indicate that soil plug height is independent of sleeve height. The results also reveal that the penetration of straight piles is closer to unplugged state than the sleeved piles. The results of incremental filling ratio and plug length ratio also indicate that the degree of soil plugging is affected by the sleeve height. 展开更多
关键词 bearing capacity inner frictional resistance inner sleeve open-ended piles soil plugging
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More Banks Will Join Open-end Fund Market
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《World Economy & China》 SCIE 2001年第2期7-7,共1页
关键词 OPEN More Banks Will Join open-end Fund Market
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木棉碳气凝胶的制备及其吸附化学污染物的研究 被引量:1
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作者 张颖 陈博 +2 位作者 戴月 杨苏祥 罗志敏 《福建师范大学学报(自然科学版)》 北大核心 2025年第2期104-109,共6页
基于生物质木棉制备碳气凝胶,介绍了从材料制备、表征到应用性能探究的开放式综合性研究型实验。通过扫描电子显微镜和红外光谱仪进行形貌与结构表征,利用热重分析仪对木棉样品进行热重分析,通过元素分析仪测定碳气凝胶各元素含量与比例... 基于生物质木棉制备碳气凝胶,介绍了从材料制备、表征到应用性能探究的开放式综合性研究型实验。通过扫描电子显微镜和红外光谱仪进行形貌与结构表征,利用热重分析仪对木棉样品进行热重分析,通过元素分析仪测定碳气凝胶各元素含量与比例,并探讨了碳气凝胶对常见化学污染物的吸附性能。结果表明,木棉经过高温碳化,含氧功能基团逐渐失去,生成的碳气凝胶中纤维的中空结构并未消失,但纤维的直径减小。碳气凝胶对环己烷、氯仿和乙醇展现了优异的吸附能力且可重复使用。实验通过科教融合拓宽学生知识体系,培养综合科研能力,为材料化学相关领域复合型拔尖创新人才培养提供有益的参考和借鉴。 展开更多
关键词 碳气凝胶 木棉 吸附剂 化学污染物处理 开放式综合性研究型实验
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露天煤矿端帮压覆资源回收方法研究现状与展望 被引量:5
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作者 周伟 田涯 +3 位作者 王知乐 才庆祥 涂立啸 李蕊 《采矿与安全工程学报》 北大核心 2025年第3期497-509,共13页
我国露天煤矿产量现已突破10亿t,约占全国产煤总量的23%,已步入世界露天采煤大国行列。多数露天煤矿开采程序为分区开采,并在采场内进行排土回填,开采方向两侧的端帮边坡随工作帮与内排土场推进而呈现出“形成—掩埋—消亡”的周期性演... 我国露天煤矿产量现已突破10亿t,约占全国产煤总量的23%,已步入世界露天采煤大国行列。多数露天煤矿开采程序为分区开采,并在采场内进行排土回填,开采方向两侧的端帮边坡随工作帮与内排土场推进而呈现出“形成—掩埋—消亡”的周期性演化规律。端帮下压覆的煤层在被内排土场掩埋后难以二次开采,导致该部分资源永久浪费,国内外学者已就回收端帮压煤做出了积极探索与实践。对靠帮开采、端帮采煤、相邻采区(采场)协调开采这3类主要方法展开综述,介绍了方法形成的背景与发展历程,归纳了方法应用场景,回顾了方法研究成果,讨论了方法所面临的技术难点,提出了靠帮开采与端帮采煤技术的研究方向,并结合国内露天开采行业发展方向,提出了5种端帮压煤回收构想,为资源进一步回收利用提供了研究思路。 展开更多
关键词 露天煤矿 端帮资源 靠帮开采 端帮采煤 协调开采 采区留沟 采场贯通
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相邻露天矿端帮断层带地质灾害隐患体治理方案 被引量:2
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作者 刘光伟 涂俊雄 +3 位作者 马显帅 姚勇 王亮 赵美玲 《煤炭科学技术》 北大核心 2025年第2期314-328,共15页
在我国大型露天煤田开发中,常常有多家煤炭企业进入同一煤田,各煤炭企业均是一个开发主体,对国家划拨条区煤炭资源进行开采,同时或先后建设几个露天矿。相邻露天矿在开采作业过程中所形成相邻端帮因受两矿采动影响较大,易发生滑坡灾害,... 在我国大型露天煤田开发中,常常有多家煤炭企业进入同一煤田,各煤炭企业均是一个开发主体,对国家划拨条区煤炭资源进行开采,同时或先后建设几个露天矿。相邻露天矿在开采作业过程中所形成相邻端帮因受两矿采动影响较大,易发生滑坡灾害,尤其是相邻端帮到界边坡。胜利西二号露天煤矿和乌兰图嘎锗煤露天矿为同一煤田相邻露天矿,两矿采矿权在相邻端帮处不重合,致使西二露天矿西帮和锗煤露天矿东帮之间形成一个梯形台体,受下覆断层破碎带及两矿作业影响,台体内出现底臌、裂缝、断层垮塌等地质灾害迹象。随着两矿剥采工程发展及对相邻端帮进行靠帮作业,相邻端帮到界边坡间将产生一个更为高陡的不规则台体,为避免灾害性滑坡发生,对该高陡台体稳定性分析基础上,提出“整体挖除回填”的治理方式并确定具体的治理方案。该治理方案明确“整体挖除回填”治理方式作业范围,设计了治理完成时清理工作帮及内排土场边坡形态,可彻底消除地质灾害隐患,并释放西二露天矿内排空间931.83万m~3,锗煤露天矿内排空间764.69万m~3,两矿可继续实现完全内排。本研究成果为西二、锗煤露天矿相邻端帮断层带地质灾害隐患体治理提供必要的理论依据和技术支撑,同时为类似同一煤田相邻露天矿实施地质灾害治理提供了相关经验。 展开更多
关键词 露天煤矿 相邻端帮 断层破碎带 地质灾害防治 边坡稳定性分析
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海上锤击沉桩过程对既有引桥影响研究 被引量:1
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作者 薄其睿 李成川 +2 位作者 尚文昌 高昌 刘俊伟 《交通科学与工程》 2025年第2期86-96,共11页
【目的】探讨海上大直径开口钢管桩锤击沉桩对邻近既有引桥的影响,确保施工安全与质量,维护引桥稳定。【方法】采用高精度现场监测技术,对沉桩过程中引桥的振动、水平位移进行实时监测。【结果】既有引桥主要受竖直方向的振动和顺桥方... 【目的】探讨海上大直径开口钢管桩锤击沉桩对邻近既有引桥的影响,确保施工安全与质量,维护引桥稳定。【方法】采用高精度现场监测技术,对沉桩过程中引桥的振动、水平位移进行实时监测。【结果】既有引桥主要受竖直方向的振动和顺桥方向水平位移的影响,尤其是振源附近的桥墩,其响应更为显著。通过多元线性回归分析,揭示了振动速度峰值与打桩距离、贯入深度之间的关系。具体来说,在振源附近,打桩距离和贯入深度的回归系数分别为-0.635和0.568,表明振动速度随着打桩距离的增大而减小,随着贯入深度的增大而增大。相反,在远离振源的区域,打桩距离和贯入深度的回归系数分别为0.678和-0.422,表明振动速度随着打桩距离的增大而增大,随着贯入深度的增加而减小。此外,引桥的水平位移也随着打桩距离的增大而减小,尤其是在振源附近的桥墩,其水平位移更为显著。水平位移随贯入深度的增加呈先增大后减少的变化趋势,特别是在桩基入岩时,水平位移达到了峰值。【结论】揭示了土层-结构相互作用下位移与振动的复杂性,可为海上沉桩施工提供实时数据支持,也可为类似工程的安全控制与质量保障提供科学依据。 展开更多
关键词 锤击沉桩 大直径开口管桩 现场监测 既有引桥影响 多元线性回归
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开绕组电机驱动系统剩余矢量重构容错策略研究
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作者 朱月婷 杨淑英 +2 位作者 张禹风 张兴 王涵宇 《电源学报》 北大核心 2025年第8期235-245,共11页
针对独立直流母线开绕组电机驱动系统单开关管开路故障提出1种剩余矢量重构策略,旨在不附加任何硬件的情况下实现容错控制,且剩余电压矢量得到最大化利用,以最大限度提升容错运行时驱动系统的运行性能。针对容错调制策略在矢量合成过程... 针对独立直流母线开绕组电机驱动系统单开关管开路故障提出1种剩余矢量重构策略,旨在不附加任何硬件的情况下实现容错控制,且剩余电压矢量得到最大化利用,以最大限度提升容错运行时驱动系统的运行性能。针对容错调制策略在矢量合成过程中出现开关管工作不均衡的问题,进一步利用了“相占空比法”对开关序列进行了优化,实现了开关管的工作状态均衡。最后,对容错策略的调制运行效果进行了深入分析,并对故障后系统运行性能进行了硬件在环实验验证。 展开更多
关键词 独立直流母线开绕组系统 剩余矢量重构策略 容错控制 相占空比法
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延时开启趾端滑套全尺寸样机综合性分析
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作者 陈福禄 李玮 +3 位作者 曹梦雨 马天驰 宁网络 张明秀 《科学技术与工程》 北大核心 2025年第31期13360-13366,共7页
为了解决青山口组页岩油井内普通常规压差式趾端滑套无法满足全井筒试压的问题,基于常规压差式滑套的开启原理,设计了满足全井筒试压条件的延时开启趾端滑套样机。利用数值模拟和室内试验手段进行了验证分析。结果表明:①该工具内受液压... 为了解决青山口组页岩油井内普通常规压差式趾端滑套无法满足全井筒试压的问题,基于常规压差式滑套的开启原理,设计了满足全井筒试压条件的延时开启趾端滑套样机。利用数值模拟和室内试验手段进行了验证分析。结果表明:①该工具内受液压110 MPa,其内最大应力值为835.935 MPa,小于材料许用应力930 MPa,工具满足设计要求;②当延时阀喷嘴直径0.5~0.6 mm时,数值模拟与理论计算结果较为接近;③当剪销个数小于等于5时,该种滑套开启压力试验值近似等于其理论计算值,且相对误差值最小为1.64%;当剪销个数为6,加装延时机构时,工具剪断销钉后能够延时28.3 min开启,与预设值相对误差仅5.67%。 展开更多
关键词 全井筒试压 延时开启 趾端滑套 页岩油井 延时机构
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基于CFD的某型集装箱船机舱主风道通风数值模拟
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作者 蔡建林 顾晓波 高霆 《造船技术》 2025年第2期18-23,84,共7页
为改善船舶机舱主风道的通风性能,以某型集装箱船机舱主风道为研究对象。在开口板上设置导流结构以优化内部通风。引入拓扑优化概念。对主风道内的开口板进行应力集中分析,将开口板的应力集中问题等效为有限宽度钢板两端受拉的应力集中... 为改善船舶机舱主风道的通风性能,以某型集装箱船机舱主风道为研究对象。在开口板上设置导流结构以优化内部通风。引入拓扑优化概念。对主风道内的开口板进行应力集中分析,将开口板的应力集中问题等效为有限宽度钢板两端受拉的应力集中问题,并基于Ansys Workbench软件对其进行拓扑优化,得到拓扑优化型开口板。基于计算流体力学(Computational Fluid Dynamics,CFD)技术,对设置原始型、导流型和拓扑优化型等3种开口板型式的主风道进行通风数值模拟,分别从主风道内的流场、压力和出风口流速等方面进行对比,分析气流在内部障碍情况下的运动特性,为集装箱船机舱主风道内部通风优化提供参考。 展开更多
关键词 集装箱船 机舱 主风道 通风 数值模拟 计算流体力学 开口板 中间截面参数
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鱼源无乳链球菌整合性接合元件ICESag1535的生物信息特征,剪切、环化活性与流行现状
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作者 车行 卢燕播 +5 位作者 王承德 罗敏意 黎果 张德峰 林蠡 赵丽娟 《水产学报》 北大核心 2025年第5期158-168,共11页
[目的]揭示水生动物源链球菌ICEs分子生物学特点、水平转移能力、ICEs流行现状,预测ICEs水平扩散的受体范围及对病原菌致病的影响。[方法]在NCBI数据库中检索ICEs特征元件,使用生物信息学方法对基因组进行分析,并鉴定ICE的结构特征、主... [目的]揭示水生动物源链球菌ICEs分子生物学特点、水平转移能力、ICEs流行现状,预测ICEs水平扩散的受体范围及对病原菌致病的影响。[方法]在NCBI数据库中检索ICEs特征元件,使用生物信息学方法对基因组进行分析,并鉴定ICE的结构特征、主要元件功能,利用分子生物学技术验证ICE在微生物间的水平转移能力并调查流行情况。[结果]筛选出尼罗罗非鱼无乳链球菌WC1535基因组中存在一个完整的ICE位于mutT基因内,命名为ICESag1535 mutT(简称ICESag1535)。ICESag1535全长约74.1 kb,编码73个蛋白基因,核心转移元件由ConjTn5252超家族的25个移动基因组成,属于类ICESa2603型ICEs。ICESag1535核心区包含5个外源基因插入热点区(HS),可变区(VR)含有7个转座插入元件(IS),形成3个复合型转座子,HS和VR可划分为7个主要功能区,分别与ICE的稳定、接合偶联、物质跨膜运输、应激调控、细菌素合成与输出、接合拓展和宿主黏附等功能相关。ICESag1535的3'-末端是同向串联的3个结构相似的位点特异性丝氨酸重组酶(TSPSI),对TSPSI进行聚类分析发现,尼罗罗非鱼、美洲牛蛙和尖吻鲈分离菌株编码的TSPSI几乎完全相同,都位于类ICESa2603型ICEs内,病原菌分布覆盖北半球水域。对不同来源ICEs同源性分析,发现人、猪、鱼分离菌株携带的类ICESa2603型ICEs,其接合模式皆为ConjTn5252型,可跨种扩散。调查类ICESa2603型ICEs的流行情况,2014—2016年分离的链球菌中携带比例为78.8%,而2020—2021年分离菌株的携带率为96.3%,呈上升趋势。验证ICESag1535的水平转移能力,检测到ICESag1535从WC1535基因组自我剪切,形成环化中间体,然后插入在人链球菌Sag158基因组mutT内,鉴定了转移过程中attL、attR、attB、oriT的位点特征;ICE整合入Sag158后,在TSPSI与attR位点之间检测到IS30插入元件。[结论]本研究鉴定的ICESag1535是具备自我切除和环化活性的完整ICE,可跨种扩散;可变区多个复合转座子,赋予ICE形成多重水平转移机制的能力,增加流行多样性;缘于IS的募集特性使ICE呈开放【目的】揭示水生动物源链球菌ICEs分子生物学特点、水平转移能力、ICEs流行现状,预测ICEs水平扩散的受体范围及对病原菌致病的影响。【方法】在NCBI数据库中检索ICEs特征元件,使用生物信息学方法对基因组进行分析,并鉴定ICE的结构特征、主要元件功能,利用分子生物学技术验证ICE在微生物间的水平转移能力并调查流行情况。【结果】筛选出尼罗罗非鱼无乳链球菌WC1535基因组中存在一个完整的ICE位于mutT基因内,命名为ICESag1535mutT(简称ICESag1535)。ICESag1535全长约74.1 kb,编码73个蛋白基因,核心转移元件由Conj Tn5252超家族的25个移动基因组成,属于类ICESa2603型ICEs。ICESag1535核心区包含5个外源基因插入热点区(HS),可变区(VR)含有7个转座插入元件(IS),形成3个复合型转座子,HS和VR可划分为7个主要功能区,分别与ICE的稳定、接合偶联、物质跨膜运输、应激调控、细菌素合成与输出、接合拓展和宿主黏附等功能相关。ICESag1535的3′-末端是同向串联的3个结构相似的位点特异性丝氨酸重组酶(TSPSI),对TSPSI进行聚类分析发现,尼罗罗非鱼、美洲牛蛙和尖吻鲈分离菌株编码的TSPSI几乎完全相同,都位于类ICESa2603型ICEs内,病原菌分布覆盖北半球水域。对不同来源ICEs同源性分析,发现人、猪、鱼分离菌株携带的类ICESa2603型ICEs,其接合模式皆为Conj Tn5252型,可跨种扩散。调查类ICESa2603型ICEs的流行情况,2014—2016年分离的链球菌中携带比例为78.8%,而2020—2021年分离菌株的携带率为96.3%,呈上升趋势。验证ICESag1535的水平转移能力,检测到ICESag1535从WC1535基因组自我剪切,形成环化中间体,然后插入在人链球菌Sag158基因组mutT内,鉴定了转移过程中attL、attR、attB、oriT的位点特征;ICE整合入Sag158后,在TSPSI与attR位点之间检测到IS30插入元件。【结论】本研究鉴定的ICESag1535是具备自我切除和环化活性的完整ICE,可跨种扩散;可变区多个复合转座子,赋予ICE形成多重水平转移机制的能力,增加流行多样性;缘于IS的募集特性使ICE呈开放式进化特点,赋予宿主微生物不断增强的适应能力和扩散性致病能力。本研究内容对微生物安全、生物进化与遗传资源研究具有重要意义。 展开更多
关键词 尼罗罗非鱼 无乳链球菌 ICE_Sag1535_mutT Conj_(Tn5252) 三串联位点特异性丝氨酸重组酶 水平基因转移 跨种扩散 开放式进化
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