The markers of oocyte quality have remained a major controversy in the field of embryology due to the subjectivity of the different methods of oocyte assessment. Various scholars use oocyte quality and oocyte competen...The markers of oocyte quality have remained a major controversy in the field of embryology due to the subjectivity of the different methods of oocyte assessment. Various scholars use oocyte quality and oocyte competence interchangeably. Oocyte quality can be defined as the overall health of an oocyte whereas oocyte competence refers to the ability of an oocyte to be fertilized and develop into a healthy embryo. Diminished oocyte quality is believed to be a result of alterations in oocyte growth and maturation processes that stem from several pelvic and systemic factors before and after oocyte retrieval. In this review, we focus on the morphological and nonmorphological markers of oocyte quality. Strict restrictions that limit the number of oocytes fertilized in various countries have triggered researchers around the world to come up with the most appropriate and noninvasive markers that enhance oocyte selection and optimize IVF outcomes. PubMed, Google Scholar, and the Cochrane Library were used to search for peer-reviewed, original articles about oocyte quality markers. The review was written in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Morphological markers are commonly used, but they are subjective, and no single marker can be used exclusively to predict oocyte competence and subsequent embryonic development potential. Furthermore, transcriptomics of differentially expressed genes in cumulus cells and assessment of metabolomics and other contents of follicular fluid have shown greater precision. However, their specificity to the different quality determinants needs further research.展开更多
With the development of human assisted reproductive technology(ART),an objective,accurate,and non-invasive method to assess the quality and viability of oocytes and embryos remains one of the most significant goals.Gr...With the development of human assisted reproductive technology(ART),an objective,accurate,and non-invasive method to assess the quality and viability of oocytes and embryos remains one of the most significant goals.Granulosa cells(GCs)play an essential role in oocyte development.GCs can differentiate into mural GCs(MGCs)and cumulus cells(CCs)under the influence of oocytes.MGCs promote the growth and development of follicles by secreting cytokines and steroid hormones.Simultaneously,CCs can form cumulus-oocyte complexes to communicate with oocytes through gap junctions and promote oocyte growth and maturation.Seeking suitable biomarkers in GCs provides a direction for the non-invasive assessment of oocyte and embryo abilities during ART procedures.To date,only a few studies have investigated potentially effective GC biomarkers during ART processes,such as the apoptosis of GCs,transcriptomic characteristics of GCs,quality and quantity of mitochondria in GCs,and telomere length of such cells.These are potential reference indices for screening high-quality oocytes and embryos.Independent studies on MGCs and CCs can provide more effective results.Although there is scope for optimization and improvement,the results have become increasingly accurate with the constant advances in technology.Due to the heterogeneity of the study population and technical limitations,clinical tests for GCs cannot be performed as part of routine tests,but their prospects are promising.This article reviews the biomarkers that have been studied in MGCs and CCs.展开更多
文摘The markers of oocyte quality have remained a major controversy in the field of embryology due to the subjectivity of the different methods of oocyte assessment. Various scholars use oocyte quality and oocyte competence interchangeably. Oocyte quality can be defined as the overall health of an oocyte whereas oocyte competence refers to the ability of an oocyte to be fertilized and develop into a healthy embryo. Diminished oocyte quality is believed to be a result of alterations in oocyte growth and maturation processes that stem from several pelvic and systemic factors before and after oocyte retrieval. In this review, we focus on the morphological and nonmorphological markers of oocyte quality. Strict restrictions that limit the number of oocytes fertilized in various countries have triggered researchers around the world to come up with the most appropriate and noninvasive markers that enhance oocyte selection and optimize IVF outcomes. PubMed, Google Scholar, and the Cochrane Library were used to search for peer-reviewed, original articles about oocyte quality markers. The review was written in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Morphological markers are commonly used, but they are subjective, and no single marker can be used exclusively to predict oocyte competence and subsequent embryonic development potential. Furthermore, transcriptomics of differentially expressed genes in cumulus cells and assessment of metabolomics and other contents of follicular fluid have shown greater precision. However, their specificity to the different quality determinants needs further research.
基金Beijing Natural Science Foundation(7202052)Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support(XMLX201825)+1 种基金National Natural Science Foundation of China(81471511)New Star Personnel Training Plan of Chao-Yang Hospital(to WHZ CYXX-2017-19)。
文摘With the development of human assisted reproductive technology(ART),an objective,accurate,and non-invasive method to assess the quality and viability of oocytes and embryos remains one of the most significant goals.Granulosa cells(GCs)play an essential role in oocyte development.GCs can differentiate into mural GCs(MGCs)and cumulus cells(CCs)under the influence of oocytes.MGCs promote the growth and development of follicles by secreting cytokines and steroid hormones.Simultaneously,CCs can form cumulus-oocyte complexes to communicate with oocytes through gap junctions and promote oocyte growth and maturation.Seeking suitable biomarkers in GCs provides a direction for the non-invasive assessment of oocyte and embryo abilities during ART procedures.To date,only a few studies have investigated potentially effective GC biomarkers during ART processes,such as the apoptosis of GCs,transcriptomic characteristics of GCs,quality and quantity of mitochondria in GCs,and telomere length of such cells.These are potential reference indices for screening high-quality oocytes and embryos.Independent studies on MGCs and CCs can provide more effective results.Although there is scope for optimization and improvement,the results have become increasingly accurate with the constant advances in technology.Due to the heterogeneity of the study population and technical limitations,clinical tests for GCs cannot be performed as part of routine tests,but their prospects are promising.This article reviews the biomarkers that have been studied in MGCs and CCs.
