Objective To evaluate the application value of rescue ICSI in fertilization failure after conventional IVF and choose the best rescue window before oocyte aging according to the results of rescue ICSI performed in dif...Objective To evaluate the application value of rescue ICSI in fertilization failure after conventional IVF and choose the best rescue window before oocyte aging according to the results of rescue ICSI performed in different time. Methods The data of 93 IVF cycles were analyzed retrospectively. Rescue ICSI was performed in these cycles after conventional IVF failure. Because of the different rescue time, these cycles were divided into two groups： early rescue group （group A, 77 cycles, rescue ICSI performed 4-8 h after conventional IVF） and late rescue group （group B, 16 cycles, rescue ICSI performed 20-22 h after conventional IVF）. Results There were no statistically significant differences in age of female, duration of infertility, number of oocytes retrieved every cycle. The normal fertilization rate, pregnancy rate and implantation rate were decreased in group B compared with those in group A （P〈0.05）. In group A, the normal fertilization rate of rescue ICSI performed 4-6 h after conventional IVF （group A1） was increased compared with that of rescue ICSI performed 6-8 h （including 6 h） after conventional IVF （group A2）（66.5% vs 55.9%）（P〈0.05）; while the abnormal fertilization rate in group A1 was decreased compared with group A2 （9.0% vs 14.4%）（P〈0.05）. Clinical pregnancy rate was slight higher in group A1 than in group A2, though this failed to be significantly different. Conclusion Rescue ICSI is effective if fertilization was failure after conventional IVF, the most important thing is to choose the reasonable rescue window before oocyte aging when ICSI is performed.展开更多
It is well established that the decline in female reproductive outcomes is related to postovulatory aging of oocytes and advanced maternal age.Poor oocyte quality is correlated with compromised genetic integrity and e...It is well established that the decline in female reproductive outcomes is related to postovulatory aging of oocytes and advanced maternal age.Poor oocyte quality is correlated with compromised genetic integrity and epigenetic changes during the oocyte aging process.Here,we review the epigenetic alterations,mainly focused on DNA methylation,histone acetylation and methylation associated with postovulatory oocyte aging as well as advanced maternal age.Furthermore,we address the underlying epigenetic mechanisms that contribute to the decline in oocyte quality during oocyte aging.展开更多
The decline in oocyte quality and developmental potential with female reproductive aging is well recognized,yet the underlying mechanisms remain insufficiently investigated.In this study,an integrative analysis of tra...The decline in oocyte quality and developmental potential with female reproductive aging is well recognized,yet the underlying mechanisms remain insufficiently investigated.In this study,an integrative analysis of transcriptomes and morphologies of individual oocytes from young and aged mice identifies morphologically defective aged oocytes with distinct transcriptomic features.Further analysis demonstrates that both apoptotic and ferroptotic pathways are activated in the defective aged oocytes,and simultaneously blocking both pathways reverses the defective morphology to the largest extent.The Plat gene,which encodes tissue-type plasminogen activator(t PA),is downregulated with oocyte aging,and Plat knockdown increases oocyte susceptibility to both apoptosis and ferroptosis.Mechanistically,t PA functions as an upstream signaling molecule for Erk1/2 activation by interacting with particular phosphorylation kinases such as Alk.Consequently,Plat loss downregulates Erk1/2 pathway activity in oocytes,leading to degeneration through PCD.Supplementing exogenous t PA in vitro oocyte maturation cultures reduces the defect rate of aged oocytes,thereby improving oocyte quality and developmental potential.Collectively,Plat plays a pivotal role in protecting aged mouse oocytes from PCD,and t PA supplementation may serve as a potential clinical strategy to enhance oocyte quality in females of advanced maternal age.展开更多
文摘Objective To evaluate the application value of rescue ICSI in fertilization failure after conventional IVF and choose the best rescue window before oocyte aging according to the results of rescue ICSI performed in different time. Methods The data of 93 IVF cycles were analyzed retrospectively. Rescue ICSI was performed in these cycles after conventional IVF failure. Because of the different rescue time, these cycles were divided into two groups： early rescue group （group A, 77 cycles, rescue ICSI performed 4-8 h after conventional IVF） and late rescue group （group B, 16 cycles, rescue ICSI performed 20-22 h after conventional IVF）. Results There were no statistically significant differences in age of female, duration of infertility, number of oocytes retrieved every cycle. The normal fertilization rate, pregnancy rate and implantation rate were decreased in group B compared with those in group A （P〈0.05）. In group A, the normal fertilization rate of rescue ICSI performed 4-6 h after conventional IVF （group A1） was increased compared with that of rescue ICSI performed 6-8 h （including 6 h） after conventional IVF （group A2）（66.5% vs 55.9%）（P〈0.05）; while the abnormal fertilization rate in group A1 was decreased compared with group A2 （9.0% vs 14.4%）（P〈0.05）. Clinical pregnancy rate was slight higher in group A1 than in group A2, though this failed to be significantly different. Conclusion Rescue ICSI is effective if fertilization was failure after conventional IVF, the most important thing is to choose the reasonable rescue window before oocyte aging when ICSI is performed.
基金supported in part by the National Natural Science Foundation of China (Grant No. 81100422)National Basic Research Program of China (Grant Nos. 2012CB944404 and 2011CB944501)
文摘It is well established that the decline in female reproductive outcomes is related to postovulatory aging of oocytes and advanced maternal age.Poor oocyte quality is correlated with compromised genetic integrity and epigenetic changes during the oocyte aging process.Here,we review the epigenetic alterations,mainly focused on DNA methylation,histone acetylation and methylation associated with postovulatory oocyte aging as well as advanced maternal age.Furthermore,we address the underlying epigenetic mechanisms that contribute to the decline in oocyte quality during oocyte aging.
基金supported by the National Natural Science Foundation of China(92374117,32170742,and 32100681)the National Key Research and Development Program of China(2025YFC2708200,2022YFC2702500,2025YFC2708001,and 2024YFC2706900)+2 种基金the Natural Science Foundation of Jiangsu Province(BK20220201)the State Key Laboratory of Reproductive Medicine and Offspring Health(SKLRM-2024B9)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX24_2010 and KYCX24_2006)。
文摘The decline in oocyte quality and developmental potential with female reproductive aging is well recognized,yet the underlying mechanisms remain insufficiently investigated.In this study,an integrative analysis of transcriptomes and morphologies of individual oocytes from young and aged mice identifies morphologically defective aged oocytes with distinct transcriptomic features.Further analysis demonstrates that both apoptotic and ferroptotic pathways are activated in the defective aged oocytes,and simultaneously blocking both pathways reverses the defective morphology to the largest extent.The Plat gene,which encodes tissue-type plasminogen activator(t PA),is downregulated with oocyte aging,and Plat knockdown increases oocyte susceptibility to both apoptosis and ferroptosis.Mechanistically,t PA functions as an upstream signaling molecule for Erk1/2 activation by interacting with particular phosphorylation kinases such as Alk.Consequently,Plat loss downregulates Erk1/2 pathway activity in oocytes,leading to degeneration through PCD.Supplementing exogenous t PA in vitro oocyte maturation cultures reduces the defect rate of aged oocytes,thereby improving oocyte quality and developmental potential.Collectively,Plat plays a pivotal role in protecting aged mouse oocytes from PCD,and t PA supplementation may serve as a potential clinical strategy to enhance oocyte quality in females of advanced maternal age.
