Objective To evaluate the application value of rescue ICSI in fertilization failure after conventional IVF and choose the best rescue window before oocyte aging according to the results of rescue ICSI performed in dif...Objective To evaluate the application value of rescue ICSI in fertilization failure after conventional IVF and choose the best rescue window before oocyte aging according to the results of rescue ICSI performed in different time. Methods The data of 93 IVF cycles were analyzed retrospectively. Rescue ICSI was performed in these cycles after conventional IVF failure. Because of the different rescue time, these cycles were divided into two groups： early rescue group （group A, 77 cycles, rescue ICSI performed 4-8 h after conventional IVF） and late rescue group （group B, 16 cycles, rescue ICSI performed 20-22 h after conventional IVF）. Results There were no statistically significant differences in age of female, duration of infertility, number of oocytes retrieved every cycle. The normal fertilization rate, pregnancy rate and implantation rate were decreased in group B compared with those in group A （P〈0.05）. In group A, the normal fertilization rate of rescue ICSI performed 4-6 h after conventional IVF （group A1） was increased compared with that of rescue ICSI performed 6-8 h （including 6 h） after conventional IVF （group A2）（66.5% vs 55.9%）（P〈0.05）; while the abnormal fertilization rate in group A1 was decreased compared with group A2 （9.0% vs 14.4%）（P〈0.05）. Clinical pregnancy rate was slight higher in group A1 than in group A2, though this failed to be significantly different. Conclusion Rescue ICSI is effective if fertilization was failure after conventional IVF, the most important thing is to choose the reasonable rescue window before oocyte aging when ICSI is performed.展开更多
It is well established that the decline in female reproductive outcomes is related to postovulatory aging of oocytes and advanced maternal age.Poor oocyte quality is correlated with compromised genetic integrity and e...It is well established that the decline in female reproductive outcomes is related to postovulatory aging of oocytes and advanced maternal age.Poor oocyte quality is correlated with compromised genetic integrity and epigenetic changes during the oocyte aging process.Here,we review the epigenetic alterations,mainly focused on DNA methylation,histone acetylation and methylation associated with postovulatory oocyte aging as well as advanced maternal age.Furthermore,we address the underlying epigenetic mechanisms that contribute to the decline in oocyte quality during oocyte aging.展开更多
文摘Objective To evaluate the application value of rescue ICSI in fertilization failure after conventional IVF and choose the best rescue window before oocyte aging according to the results of rescue ICSI performed in different time. Methods The data of 93 IVF cycles were analyzed retrospectively. Rescue ICSI was performed in these cycles after conventional IVF failure. Because of the different rescue time, these cycles were divided into two groups： early rescue group （group A, 77 cycles, rescue ICSI performed 4-8 h after conventional IVF） and late rescue group （group B, 16 cycles, rescue ICSI performed 20-22 h after conventional IVF）. Results There were no statistically significant differences in age of female, duration of infertility, number of oocytes retrieved every cycle. The normal fertilization rate, pregnancy rate and implantation rate were decreased in group B compared with those in group A （P〈0.05）. In group A, the normal fertilization rate of rescue ICSI performed 4-6 h after conventional IVF （group A1） was increased compared with that of rescue ICSI performed 6-8 h （including 6 h） after conventional IVF （group A2）（66.5% vs 55.9%）（P〈0.05）; while the abnormal fertilization rate in group A1 was decreased compared with group A2 （9.0% vs 14.4%）（P〈0.05）. Clinical pregnancy rate was slight higher in group A1 than in group A2, though this failed to be significantly different. Conclusion Rescue ICSI is effective if fertilization was failure after conventional IVF, the most important thing is to choose the reasonable rescue window before oocyte aging when ICSI is performed.
基金supported in part by the National Natural Science Foundation of China (Grant No. 81100422)National Basic Research Program of China (Grant Nos. 2012CB944404 and 2011CB944501)
文摘It is well established that the decline in female reproductive outcomes is related to postovulatory aging of oocytes and advanced maternal age.Poor oocyte quality is correlated with compromised genetic integrity and epigenetic changes during the oocyte aging process.Here,we review the epigenetic alterations,mainly focused on DNA methylation,histone acetylation and methylation associated with postovulatory oocyte aging as well as advanced maternal age.Furthermore,we address the underlying epigenetic mechanisms that contribute to the decline in oocyte quality during oocyte aging.
