OBJECTIVE:To investigate the efficacy of the extract from Ononis spinosa L.(O.spinosa)on ethanol-induced gastric ulcer in rats.METHODS:Phytochemical constituents of the extract from O.spinosa were analyzed using liqui...OBJECTIVE:To investigate the efficacy of the extract from Ononis spinosa L.(O.spinosa)on ethanol-induced gastric ulcer in rats.METHODS:Phytochemical constituents of the extract from O.spinosa were analyzed using liquid chromatography-mass spectrometry.Rats were classified into 4 equal groups;ulcer control received oral vehicle;positive control was administered with 40 mg/kg esomeprazole(standard drug)and 2 groups received 0.5 and 1 g/kg of O.spinosa extract,respectively.Gastric ulcer was induced by absolute ethanol(5 mL/kg)orally to all groups.Measurement of ulcer index,cyclooxygenase-2(COX-2)expression and determination of total glutathione level in gastric mucosa were performed.RESULTS:Oral administration of the extract from O.spinosa at doses 0.5 and 1 g/kg lowered the ulcer indices by 80.39%and 98.71%,respectively,compared to 67.89%by esomeprazole(40 mg/kg).Histologically,treatment with the extract decreased necrosis and hemorrhage in mucosa and edema and infiltration by inflammatory cells in submucosa.Using immunohistochemical technique,it was demonstrated that COX-2 expression increased in mucosa of animals treated with the extract as well as by esomeprazole.O.spinosa and esomeprazole increased total glutathione level in the stomach compared to control.Ononin was the major compound of the extract followed by trifolirhizin,myricitrin,gentisic acid,cycloartenol and quercetin.CONCLUSION:The present study demonstrated that the extract from O.spinosa was able to protect gastric mucosa from ethanol injury by at least 2 mechanisms,namely the induction of COX-2 and decreasing oxidative stress in the stomach.展开更多
Skeletal muscle atrophy,a condition associated with sarcopenia,cachexia,and various metabolic diseases,significantly impairs physical function and quality of life.This study investigates the potential of Ononis natrix...Skeletal muscle atrophy,a condition associated with sarcopenia,cachexia,and various metabolic diseases,significantly impairs physical function and quality of life.This study investigates the potential of Ononis natrix subsp.hispanica water extract,rich in quercetin,luteolin,and apigenin,to mitigate muscle atrophy in an in vitro model.Atrophy was induced in differentiated C2C12 myotubes using dexamethasone,and the effects of the extract and a mixture of its phytochemicals(QLA mixture)were evaluated on myotube morphology and key protein degradation markers.The extract significantly reduced dexamethasone-induced myotube diameter loss from 28%to 13%-15.4%(p<0.001).Similarly,the QLA mixture limited diameter loss to 14.6%,maintaining a morphology closer to the control.The extract and QLA mixture attenuated the dexamethasone-induced upregulation of muscle-specific ubiquitin ligases MAFbx and MuRF1,with mRNA levels reduced by 2.1-2.4-fold and 1.9-2.4-fold,respectively(p<0.001).Western blot analysis validated the reduction in MAFbx protein levels.Molecular docking studies demonstrated robust binding interactions of quercetin,luteolin,and apigenin with TGFBR1 and IKBKB,showing binding energies as low as-10.1 kcal/mol.Notably,luteolin formed six hydrogen bonds with TGFBR1,while apigenin displayed four hydrogen bonds with IKBKB,underscoring their strong inhibitor potential.KEGG pathway enrichment analysis highlighted the involvement of PI3K-Akt,NF-κB,and FoxO signaling pathways,elucidating the extract’s multi-target mechanisms.These findings establish the therapeutic promise of O.natrix extract as a natural agent for managing skeletal muscle atrophy and improving muscle health.展开更多
文摘OBJECTIVE:To investigate the efficacy of the extract from Ononis spinosa L.(O.spinosa)on ethanol-induced gastric ulcer in rats.METHODS:Phytochemical constituents of the extract from O.spinosa were analyzed using liquid chromatography-mass spectrometry.Rats were classified into 4 equal groups;ulcer control received oral vehicle;positive control was administered with 40 mg/kg esomeprazole(standard drug)and 2 groups received 0.5 and 1 g/kg of O.spinosa extract,respectively.Gastric ulcer was induced by absolute ethanol(5 mL/kg)orally to all groups.Measurement of ulcer index,cyclooxygenase-2(COX-2)expression and determination of total glutathione level in gastric mucosa were performed.RESULTS:Oral administration of the extract from O.spinosa at doses 0.5 and 1 g/kg lowered the ulcer indices by 80.39%and 98.71%,respectively,compared to 67.89%by esomeprazole(40 mg/kg).Histologically,treatment with the extract decreased necrosis and hemorrhage in mucosa and edema and infiltration by inflammatory cells in submucosa.Using immunohistochemical technique,it was demonstrated that COX-2 expression increased in mucosa of animals treated with the extract as well as by esomeprazole.O.spinosa and esomeprazole increased total glutathione level in the stomach compared to control.Ononin was the major compound of the extract followed by trifolirhizin,myricitrin,gentisic acid,cycloartenol and quercetin.CONCLUSION:The present study demonstrated that the extract from O.spinosa was able to protect gastric mucosa from ethanol injury by at least 2 mechanisms,namely the induction of COX-2 and decreasing oxidative stress in the stomach.
基金funded by Kastamonu University,Scientific Research Projects Coordination Unit under grant number KÜ-BAP01/2020-60.
文摘Skeletal muscle atrophy,a condition associated with sarcopenia,cachexia,and various metabolic diseases,significantly impairs physical function and quality of life.This study investigates the potential of Ononis natrix subsp.hispanica water extract,rich in quercetin,luteolin,and apigenin,to mitigate muscle atrophy in an in vitro model.Atrophy was induced in differentiated C2C12 myotubes using dexamethasone,and the effects of the extract and a mixture of its phytochemicals(QLA mixture)were evaluated on myotube morphology and key protein degradation markers.The extract significantly reduced dexamethasone-induced myotube diameter loss from 28%to 13%-15.4%(p<0.001).Similarly,the QLA mixture limited diameter loss to 14.6%,maintaining a morphology closer to the control.The extract and QLA mixture attenuated the dexamethasone-induced upregulation of muscle-specific ubiquitin ligases MAFbx and MuRF1,with mRNA levels reduced by 2.1-2.4-fold and 1.9-2.4-fold,respectively(p<0.001).Western blot analysis validated the reduction in MAFbx protein levels.Molecular docking studies demonstrated robust binding interactions of quercetin,luteolin,and apigenin with TGFBR1 and IKBKB,showing binding energies as low as-10.1 kcal/mol.Notably,luteolin formed six hydrogen bonds with TGFBR1,while apigenin displayed four hydrogen bonds with IKBKB,underscoring their strong inhibitor potential.KEGG pathway enrichment analysis highlighted the involvement of PI3K-Akt,NF-κB,and FoxO signaling pathways,elucidating the extract’s multi-target mechanisms.These findings establish the therapeutic promise of O.natrix extract as a natural agent for managing skeletal muscle atrophy and improving muscle health.
