Certificateless one-round key exchange(CL-ORKE)protocols enable each participant to share a common key with only one round of communication which greatly saves communication cost.CLORKE protocols can be applied to sce...Certificateless one-round key exchange(CL-ORKE)protocols enable each participant to share a common key with only one round of communication which greatly saves communication cost.CLORKE protocols can be applied to scenarios with limited communication,such as space communication.Although CL-ORKE protocols have been researched for years,lots of them only consider what secrets can be compromised but ignore the time when the secrets have been corrupted.In CL-ORKE protocols,the reveal of the long-term key attacks can be divided into two different attacks according to the time of the long-term key revealed:the attack to weak Forward Security(wFS)and the attack to strong Forward Security(sFS).Many CLKE protocols did not take into account the sFS property or considered sFS as wFS.In this paper,we first propose a new security model for CL-ORKE protocols which considers the sFS property as well as the Ephemeral Key Reveal attack.Then,we give a CL-ORKE protocol which is called CLORKE-SFS.CLORKE-SFS is provably secure under the proposed model provided the Elliptic Curve Computational Diffie-Hellman(ECCDH)and the Bilinear Computational Diffie-Hellman problem(BCDH)assumption hold.The security model and the protocol may give inspiration for constructing oneround key exchange protocols with perfect forward security in certificateless scenarios.展开更多
Selection of aptamers with high affinity and good specificity requires multiple rounds of alternating steps of separation and PCR amplification.Herein,we proposed a novel high-efficiency aptamers picking strategy:One-...Selection of aptamers with high affinity and good specificity requires multiple rounds of alternating steps of separation and PCR amplification.Herein,we proposed a novel high-efficiency aptamers picking strategy:One-round pressure controllable selection(OPCS).OPCS integrates four types of screening superiority,high-efficiency separation,one-round selection and PCR amplification,synchronous negative selection and targets competition.The controllable screening pressure can be achieved through two approaches,balanced competition by the regulation of protein concentration,and dominant competition by introducing a predatory protein with high concentration.In OPCS process,two proteins were co-incubated with one ssDNA library,and each protein bound its favorable sequences specifically and formed protein-ss D NA complex re spectively.Meanwhile,one protein could supply/sufferthe picking pressure of affinity and specificity to/from another,which eliminated weakly bound or unbound sequences for each other.Two complexes could be separated and collected conveniently,and aptamers for two proteins obtained synchronously with high affinity and good specificity.This strategy not only provides a more effective way for aptamers selection,but shows great potential in other ligands or drugs selection.展开更多
基金This work was supported by the National Natural Science Foundation of China(NSFC)under Grant(61902049,31960119)Joint Special Fund for Basic Research of Local Undergraduate Universities(Parts)in Yunnan Province under Grant(2018FH001-063,2018FH001-106)Dali University Innovation Team Project(ZKLX2020308).
文摘Certificateless one-round key exchange(CL-ORKE)protocols enable each participant to share a common key with only one round of communication which greatly saves communication cost.CLORKE protocols can be applied to scenarios with limited communication,such as space communication.Although CL-ORKE protocols have been researched for years,lots of them only consider what secrets can be compromised but ignore the time when the secrets have been corrupted.In CL-ORKE protocols,the reveal of the long-term key attacks can be divided into two different attacks according to the time of the long-term key revealed:the attack to weak Forward Security(wFS)and the attack to strong Forward Security(sFS).Many CLKE protocols did not take into account the sFS property or considered sFS as wFS.In this paper,we first propose a new security model for CL-ORKE protocols which considers the sFS property as well as the Ephemeral Key Reveal attack.Then,we give a CL-ORKE protocol which is called CLORKE-SFS.CLORKE-SFS is provably secure under the proposed model provided the Elliptic Curve Computational Diffie-Hellman(ECCDH)and the Bilinear Computational Diffie-Hellman problem(BCDH)assumption hold.The security model and the protocol may give inspiration for constructing oneround key exchange protocols with perfect forward security in certificateless scenarios.
基金the National Natural Science Foundation of China(Nos.21675012,21874010 and 21827810)the Young Elite Scientist Sponsorship Program of Beijing Association for Science and Technology。
文摘Selection of aptamers with high affinity and good specificity requires multiple rounds of alternating steps of separation and PCR amplification.Herein,we proposed a novel high-efficiency aptamers picking strategy:One-round pressure controllable selection(OPCS).OPCS integrates four types of screening superiority,high-efficiency separation,one-round selection and PCR amplification,synchronous negative selection and targets competition.The controllable screening pressure can be achieved through two approaches,balanced competition by the regulation of protein concentration,and dominant competition by introducing a predatory protein with high concentration.In OPCS process,two proteins were co-incubated with one ssDNA library,and each protein bound its favorable sequences specifically and formed protein-ss D NA complex re spectively.Meanwhile,one protein could supply/sufferthe picking pressure of affinity and specificity to/from another,which eliminated weakly bound or unbound sequences for each other.Two complexes could be separated and collected conveniently,and aptamers for two proteins obtained synchronously with high affinity and good specificity.This strategy not only provides a more effective way for aptamers selection,but shows great potential in other ligands or drugs selection.
