Receptor tyrosine kinases(RTKs)are biological enzymes expressed on cell membranes that can influence cellular signaling,and their overexpression in tumor cells makes them a key route to assess relevant tumor processes...Receptor tyrosine kinases(RTKs)are biological enzymes expressed on cell membranes that can influence cellular signaling,and their overexpression in tumor cells makes them a key route to assess relevant tumor processes.The development of a delivery system that targets and accumulates in RTKs overexpressing-cells at the on-target site is significant for the monitoring of tumor progression and clinical applications through longer tumor site signaling response under low injection frequency.Here,a host-vip nanoscale fluorescent probe SNI@ZIF-8 based on zeolitic imidazolate framework-8(ZIF-8)and a fluorescent probe SNI constructed from receptor tyrosine kinase inhibitor was proposed and prepared for targeting RTKs and enabling prolonged fluorescence imaging in vivo.The folded conformation of the probe SNI resulted in low background fluorescence,and the unfolding of the SNI conformation upon insertion of the RTKs active pocket showed significant fluorescence enhancement thus enabling real-time detection of RTKs.The host-vip system SNI@ZIF-8 could release vip molecules due to the presence of the enzyme,emphasizing the reporting of stable fluorescent signals over time under low injection frequency.SNI@ZIF-8 could provide a signal response on the cell membrane of RTKs overexpressing cells without interference from other substances,and provided a longer fluorescent signal than SNI at equivalent number of injections in tumor-bearing mice.The host-vip system SNI@ZIF-8,with its obvious tumor site enrichment ability and clear fluorescence imaging ability,could be successfully applied to the detection of RTKs on cell membranes in biological systems,providing a new strategy for determining the process of tumor development in clinical applications.展开更多
为解决当前行蓄洪区管理和运用存在的管理方式粗放、安全运用手段单一、信息资源管理分散、预警难以全覆盖等问题,提出建设行蓄洪区数字“一张图”,利用卫星遥感、卷帘展示、基于位置服务(Location Based Services,LBS)靶向预警、洪水...为解决当前行蓄洪区管理和运用存在的管理方式粗放、安全运用手段单一、信息资源管理分散、预警难以全覆盖等问题,提出建设行蓄洪区数字“一张图”,利用卫星遥感、卷帘展示、基于位置服务(Location Based Services,LBS)靶向预警、洪水风险推演等技术,基于数字孪生水利的总体架构,打造行蓄洪区基础信息查询、违建巡查管理、安全运用分析、通信预警指挥及运用补偿管理5个典型应用场景,并建设业务后台,提高对行蓄洪区的运行监管能力。行蓄洪区数字“一张图”建成并投入使用后,在支撑安徽省行蓄洪区日常管理及安全运用方面发挥了重要的作用,研究成果可为行蓄洪区管理及数字孪生流域防洪建设、应用,以及全生命周期管理提供可行途径和经验借鉴。展开更多
研究了核主分量分析(KPCA,Kernel Principal Component Analysis)在高分辨雷达目标特征提取与识别中的应用。首先讨论了KPCA算法原理,然后将KPCA应用于雷达目标距离像特征提取,并采用支持向量机进行分类,提出了基于核主分量分析的高分...研究了核主分量分析(KPCA,Kernel Principal Component Analysis)在高分辨雷达目标特征提取与识别中的应用。首先讨论了KPCA算法原理,然后将KPCA应用于雷达目标距离像特征提取,并采用支持向量机进行分类,提出了基于核主分量分析的高分辨雷达目标特征提取与识别方法。在核函数的选取上构造了一个组合核函数,最后用4类目标数据进行了实验,并与采用高斯核函数方法进行了比较,实验结果表明,该方法能够提高目标识别性能。展开更多
基金supported by the National Natural Science Foundation of China(Nos.22338005,21977015)。
文摘Receptor tyrosine kinases(RTKs)are biological enzymes expressed on cell membranes that can influence cellular signaling,and their overexpression in tumor cells makes them a key route to assess relevant tumor processes.The development of a delivery system that targets and accumulates in RTKs overexpressing-cells at the on-target site is significant for the monitoring of tumor progression and clinical applications through longer tumor site signaling response under low injection frequency.Here,a host-vip nanoscale fluorescent probe SNI@ZIF-8 based on zeolitic imidazolate framework-8(ZIF-8)and a fluorescent probe SNI constructed from receptor tyrosine kinase inhibitor was proposed and prepared for targeting RTKs and enabling prolonged fluorescence imaging in vivo.The folded conformation of the probe SNI resulted in low background fluorescence,and the unfolding of the SNI conformation upon insertion of the RTKs active pocket showed significant fluorescence enhancement thus enabling real-time detection of RTKs.The host-vip system SNI@ZIF-8 could release vip molecules due to the presence of the enzyme,emphasizing the reporting of stable fluorescent signals over time under low injection frequency.SNI@ZIF-8 could provide a signal response on the cell membrane of RTKs overexpressing cells without interference from other substances,and provided a longer fluorescent signal than SNI at equivalent number of injections in tumor-bearing mice.The host-vip system SNI@ZIF-8,with its obvious tumor site enrichment ability and clear fluorescence imaging ability,could be successfully applied to the detection of RTKs on cell membranes in biological systems,providing a new strategy for determining the process of tumor development in clinical applications.
文摘为解决当前行蓄洪区管理和运用存在的管理方式粗放、安全运用手段单一、信息资源管理分散、预警难以全覆盖等问题,提出建设行蓄洪区数字“一张图”,利用卫星遥感、卷帘展示、基于位置服务(Location Based Services,LBS)靶向预警、洪水风险推演等技术,基于数字孪生水利的总体架构,打造行蓄洪区基础信息查询、违建巡查管理、安全运用分析、通信预警指挥及运用补偿管理5个典型应用场景,并建设业务后台,提高对行蓄洪区的运行监管能力。行蓄洪区数字“一张图”建成并投入使用后,在支撑安徽省行蓄洪区日常管理及安全运用方面发挥了重要的作用,研究成果可为行蓄洪区管理及数字孪生流域防洪建设、应用,以及全生命周期管理提供可行途径和经验借鉴。
文摘研究了核主分量分析(KPCA,Kernel Principal Component Analysis)在高分辨雷达目标特征提取与识别中的应用。首先讨论了KPCA算法原理,然后将KPCA应用于雷达目标距离像特征提取,并采用支持向量机进行分类,提出了基于核主分量分析的高分辨雷达目标特征提取与识别方法。在核函数的选取上构造了一个组合核函数,最后用4类目标数据进行了实验,并与采用高斯核函数方法进行了比较,实验结果表明,该方法能够提高目标识别性能。
