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On-Line Privacy Behavior: Using User Interfaces for Salient Factors
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作者 Thomas Hughes-Roberts Elahe Kani-Zabihi 《Journal of Computer and Communications》 2014年第4期220-231,共12页
The problem of privacy in social networks is well documented within literature;users have pri- vacy concerns however, they consistently disclose their sensitive information and leave it open to unintended third partie... The problem of privacy in social networks is well documented within literature;users have pri- vacy concerns however, they consistently disclose their sensitive information and leave it open to unintended third parties. While numerous causes of poor behaviour have been suggested by re- search the role of the User Interface (UI) and the system itself is underexplored. The field of Per- suasive Technology would suggest that Social Network Systems persuade users to deviate from their normal or habitual behaviour. This paper makes the case that the UI can be used as the basis for user empowerment by informing them of their privacy at the point of interaction and remind- ing them of their privacy needs. The Theory of Planned Behaviour is introduced as a potential theoretical foundation for exploring the psychology behind privacy behaviour as it describes the salient factors that influence intention and action. Based on these factors of personal attitude, subjective norms and perceived control, a series of UIs are presented and implemented in con- trolled experiments examining their effect on personal information disclosure. This is combined with observations and interviews with the participants. Results from this initial, pilot experiment suggest groups with privacy salient information embedded exhibit less disclosure than the control group. This work reviews this approach as a method for exploring privacy behaviour and propos- es further work required. 展开更多
关键词 on-line privacy Social NETWORKING Site User Interface Users’ BEHAVIOR Theory of PLANNED BEHAVIOR
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NLDA non-linear regression model for preserving data privacy in wireless sensor networks 被引量:1
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作者 A.L.Sreenivasulu P.Chenna Reddy 《Digital Communications and Networks》 SCIE 2020年第1期101-107,共7页
Recently,the application of Wireless Sensor Networks(WSNs)has been increasing rapidly.It requires privacy preserving data aggregation protocols to secure the data from compromises.Preserving privacy of the sensor data... Recently,the application of Wireless Sensor Networks(WSNs)has been increasing rapidly.It requires privacy preserving data aggregation protocols to secure the data from compromises.Preserving privacy of the sensor data is a challenging task.This paper presents a non-linear regression-based data aggregation protocol for preserving privacy of the sensor data.The proposed protocol uses non-linear regression functions to represent the sensor data collected from the sensor nodes.Instead of sending the complete data to the cluster head,the sensor nodes only send the coefficients of the non-linear function.This will reduce the communication overhead of the network.The data aggregation is performed on the masked coefficients and the sink node is able to retrieve the approximated results over the aggregated data.The analysis of experiment results shows that the proposed protocol is able to minimize communication overhead,enhance data aggregation accuracy,and preserve data privacy. 展开更多
关键词 Sensor nodes Data accuracy Wireless sensor networks Data aggregation privacy preserving
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A Mass On-line Control Type Impact Inertial Piezoelectric Actuator Based on a Bionic Wheat Awn Structure
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作者 Youjia Li Shanshan Wu +5 位作者 Taofei Long Jianping Li Jijie Ma Xinhui Li Yili Hu Jianming Wen 《Journal of Bionic Engineering》 2025年第6期3058-3075,共18页
A mass on-line control type impact inertial piezoelectric actuator with a bionic wheat structure is proposed in this work.Inspired by the anisotropic friction mechanism of natural wheat awns,a bioinspired mechanism is... A mass on-line control type impact inertial piezoelectric actuator with a bionic wheat structure is proposed in this work.Inspired by the anisotropic friction mechanism of natural wheat awns,a bioinspired mechanism is used to achieve the designed driving strategy based on the asymmetric-mass control method that mimics bidirectional motion characteristics of wheat awn.A lumped parameter theoretical model is established,and the numerical simulation results have verified the designed bionic working principle and revealed the key system parameters.Experimental results show that the prototype has the bi-directional motion ability inherited from anisotropic friction of wheat awn,with theoretically infinite stroke and can easily obtain the required step displacement and velocity by conveniently adjusting the voltage.It can achieve a resolution of 0.7μm,and a forward and backward maximum velocity of 12.7μm/s and 90.72μm/s respectively.In addition,the actuator also has the advantages of good stability,control convenience,and ease of integration.Besides,the actuator is capable of adjusting motion direction via voltage,providing a significant advantage in precise bidirectional control.This study confirms that the proposed mass on-line control type actuator embodies a successful bionic translation from plant morphology to precision engineering,and adds a new member to the family of impact inertial piezoelectric actuators,which completes the last piece of the puzzle for the impact inertial driving mechanism.It promotes the further development of inertial precision driving and control technology and is expected to expand the scope of application.Future work will focus on optimizing performance and developing applications. 展开更多
关键词 BIONIC Bi-directional motion INERTIAL Mass on-line control Piezoelectric actuator
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Privacy-Preserving Personnel Detection in Substations via Federated Learning with Dynamic Noise Adaptation
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作者 Yuewei Tian Yang Su +4 位作者 Yujia Wang Lisa Guo Xuyang Wu Lei Cao Fang Ren 《Computers, Materials & Continua》 2026年第3期894-915,共22页
This study addresses the risk of privacy leakage during the transmission and sharing of multimodal data in smart grid substations by proposing a three-tier privacy-preserving architecture based on asynchronous federat... This study addresses the risk of privacy leakage during the transmission and sharing of multimodal data in smart grid substations by proposing a three-tier privacy-preserving architecture based on asynchronous federated learning.The framework integrates blockchain technology,the InterPlanetary File System(IPFS)for distributed storage,and a dynamic differential privacy mechanism to achieve collaborative security across the storage,service,and federated coordination layers.It accommodates both multimodal data classification and object detection tasks,enabling the identification and localization of key targets and abnormal behaviors in substation scenarios while ensuring privacy protection.This effectively mitigates the single-point failures and model leakage issues inherent in centralized architectures.A dynamically adjustable differential privacy mechanism is introduced to allocate privacy budgets according to client contribution levels and upload frequencies,achieving a personalized balance between model performance and privacy protection.Multi-dimensional experimental evaluations,including classification accuracy,F1-score,encryption latency,and aggregation latency,verify the security and efficiency of the proposed architecture.The improved CNN model achieves 72.34%accuracy and an F1-score of 0.72 in object detection and classification tasks on infrared surveillance imagery,effectively identifying typical risk events such as not wearing safety helmets and unauthorized intrusion,while maintaining an aggregation latency of only 1.58 s and a query latency of 80.79 ms.Compared with traditional static differential privacy and centralized approaches,the proposed method demonstrates significant advantages in accuracy,latency,and security,providing a new technical paradigm for efficient,secure data sharing,object detection,and privacy preservation in smart grid substations. 展开更多
关键词 SUBSTATION privacy preservation asynchronous federated learning CNN differential privacy
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FedDPL:Federated Dynamic Prototype Learning for Privacy-Preserving Malware Analysis across Heterogeneous Clients
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作者 Danping Niu Yuan Ping +2 位作者 Chun Guo Xiaojun Wang Bin Hao 《Computers, Materials & Continua》 2026年第3期1989-2014,共26页
With the increasing complexity of malware attack techniques,traditional detection methods face significant challenges,such as privacy preservation,data heterogeneity,and lacking category information.To address these i... With the increasing complexity of malware attack techniques,traditional detection methods face significant challenges,such as privacy preservation,data heterogeneity,and lacking category information.To address these issues,we propose Federated Dynamic Prototype Learning(FedDPL)for malware classification by integrating Federated Learning with a specifically designed K-means.Under the Federated Learning framework,model training occurs locally without data sharing,effectively protecting user data privacy and preventing the leakage of sensitive information.Furthermore,to tackle the challenges of data heterogeneity and the lack of category information,FedDPL introduces a dynamic prototype learning mechanism,which adaptively adjusts the clustering prototypes in terms of position and number.Thus,the dependency on predefined category numbers in typical K-means and its variants can be significantly reduced,resulting in improved clustering performance.Theoretically,it provides a more accurate detection of malicious behavior.Experimental results confirm that FedDPL excels in handling malware classification tasks,demonstrating superior accuracy,robustness,and privacy protection. 展开更多
关键词 Malware classification data heterogeneity federated learning CLUSTERING differential privacy
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DPIL-Traj: Differential Privacy Trajectory Generation Framework with Imitation Learning
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作者 Huaxiong Liao Xiangxuan Zhong +4 位作者 Xueqi Chen Yirui Huang Yuwei Lin Jing Zhang Bruce Gu 《Computers, Materials & Continua》 2026年第1期1530-1550,共21页
The generation of synthetic trajectories has become essential in various fields for analyzing complex movement patterns.However,the use of real-world trajectory data poses significant privacy risks,such as location re... The generation of synthetic trajectories has become essential in various fields for analyzing complex movement patterns.However,the use of real-world trajectory data poses significant privacy risks,such as location reidentification and correlation attacks.To address these challenges,privacy-preserving trajectory generation methods are critical for applications relying on sensitive location data.This paper introduces DPIL-Traj,an advanced framework designed to generate synthetic trajectories while achieving a superior balance between data utility and privacy preservation.Firstly,the framework incorporates Differential Privacy Clustering,which anonymizes trajectory data by applying differential privacy techniques that add noise,ensuring the protection of sensitive user information.Secondly,Imitation Learning is used to replicate decision-making behaviors observed in real-world trajectories.By learning from expert trajectories,this component generates synthetic data that closely mimics real-world decision-making processes while optimizing the quality of the generated trajectories.Finally,Markov-based Trajectory Generation is employed to capture and maintain the inherent temporal dynamics of movement patterns.Extensive experiments conducted on the GeoLife trajectory dataset show that DPIL-Traj improves utility performance by an average of 19.85%,and in terms of privacy performance by an average of 12.51%,compared to state-of-the-art approaches.Ablation studies further reveal that DP clustering effectively safeguards privacy,imitation learning enhances utility under noise,and the Markov module strengthens temporal coherence. 展开更多
关键词 privacy-PRESERVING trajectory generation differential privacy imitation learning Markov chain
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Information Diffusion Models and Fuzzing Algorithms for a Privacy-Aware Data Transmission Scheduling in 6G Heterogeneous ad hoc Networks
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作者 Borja Bordel Sánchez Ramón Alcarria Tomás Robles 《Computer Modeling in Engineering & Sciences》 2026年第2期1214-1234,共21页
In this paper,we propose a new privacy-aware transmission scheduling algorithm for 6G ad hoc networks.This system enables end nodes to select the optimum time and scheme to transmit private data safely.In 6G dynamic h... In this paper,we propose a new privacy-aware transmission scheduling algorithm for 6G ad hoc networks.This system enables end nodes to select the optimum time and scheme to transmit private data safely.In 6G dynamic heterogeneous infrastructures,unstable links and non-uniform hardware capabilities create critical issues regarding security and privacy.Traditional protocols are often too computationally heavy to allow 6G services to achieve their expected Quality-of-Service(QoS).As the transport network is built of ad hoc nodes,there is no guarantee about their trustworthiness or behavior,and transversal functionalities are delegated to the extreme nodes.However,while security can be guaranteed in extreme-to-extreme solutions,privacy cannot,as all intermediate nodes still have to handle the data packets they are transporting.Besides,traditional schemes for private anonymous ad hoc communications are vulnerable against modern intelligent attacks based on learning models.The proposed scheme fulfills this gap.Findings show the probability of a successful intelligent attack reduces by up to 65%compared to ad hoc networks with no privacy protection strategy when used the proposed technology.While congestion probability can remain below 0.001%,as required in 6G services. 展开更多
关键词 6G networks ad hoc networks privacy scheduling algorithms diffusion models fuzzing algorithms
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Privacy-Preserving Gender-Based Customer Behavior Analytics in Retail Spaces Using Computer Vision
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作者 Ginanjar Suwasono Adi Samsul Huda +4 位作者 Griffani Megiyanto Rahmatullah Dodit Suprianto Dinda Qurrota Aini Al-Sefy Ivon Sandya Sari Putri Lalu Tri Wijaya Nata Kusuma 《Computers, Materials & Continua》 2026年第1期1839-1861,共23页
In the competitive retail industry of the digital era,data-driven insights into gender-specific customer behavior are essential.They support the optimization of store performance,layout design,product placement,and ta... In the competitive retail industry of the digital era,data-driven insights into gender-specific customer behavior are essential.They support the optimization of store performance,layout design,product placement,and targeted marketing.However,existing computer vision solutions often rely on facial recognition to gather such insights,raising significant privacy and ethical concerns.To address these issues,this paper presents a privacypreserving customer analytics system through two key strategies.First,we deploy a deep learning framework using YOLOv9s,trained on the RCA-TVGender dataset.Cameras are positioned perpendicular to observation areas to reduce facial visibility while maintaining accurate gender classification.Second,we apply AES-128 encryption to customer position data,ensuring secure access and regulatory compliance.Our system achieved overall performance,with 81.5%mAP@50,77.7%precision,and 75.7%recall.Moreover,a 90-min observational study confirmed the system’s ability to generate privacy-protected heatmaps revealing distinct behavioral patterns between male and female customers.For instance,women spent more time in certain areas and showed interest in different products.These results confirm the system’s effectiveness in enabling personalized layout and marketing strategies without compromising privacy. 展开更多
关键词 Business intelligence customer behavior privacy-preserving analytics computer vision deep learning smart retail gender recognition heatmap privacy RCA-TVGender dataset
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Quantitative determination of cyclophosphamide in rat plasma using an on-line SPE HPLC-DAD 被引量:2
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作者 李晓娜 于宁 +5 位作者 张建美 林文斯 凌笑梅 富戈 李润涛 崔景荣 《Journal of Chinese Pharmaceutical Sciences》 CAS 2012年第2期156-161,共6页
A rapid and simple liquid chromatography method with on-line solid phase extraction was developed and validated for the quantitative determination of cyclophosphamide in rat plasma.The plasma sample was first extracte... A rapid and simple liquid chromatography method with on-line solid phase extraction was developed and validated for the quantitative determination of cyclophosphamide in rat plasma.The plasma sample was first extracted on an Acclaim? Polar Advantage II C18 guard column(PA II C18,10 mm×4.6 mm,5 μm),which was also the on-line Extraction Cartridge SPE column,by washing with 100% H2O for 1 min.The extracted sample was then eluted onto a PA II C18 column(150 mm×4.6 mm,5 μm) and separated by isocratic elution with acetonitrile-water(40:60,v/v).The mobile phase was run at a flow rate of 1.0 mL/min,and the UV detector was set at 195 nm.Retention time of cyclophosphamide was 4.3 min and the total run-time was 6 min.The linear range of the standard curve was from 1.0 to 200 μg/mL(r2 = 0.9999),and the limits of quantification and detection were 1.0 μg/mL(RSD10%,n = 5) and 0.3 μg/mL(RSD13%,n = 5),respectively.Both intra-and inter-day variations were less than 5.6%.The developed method can be used for the therapeutic drug monitoring of cyclophosphamide in the clinic. 展开更多
关键词 on-line SPE HPLC-DAD CYCLOPHOSPHAMIDE Rat plasma
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On-line SVM在船舶设备故障预测中的应用 被引量:2
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作者 许小伟 范世东 姚玉南 《武汉理工大学学报》 CAS CSCD 北大核心 2014年第9期61-67,共7页
船舶设备众多、型号多样、结构复杂,故障预测所需的有效数据偏少,而大规模的在线数据监测又受制于存储空间和计算时间的限制。利用On-line SVM建立了故障预测模型,分析了船舶设备故障预测的流程;并以某船舶主机的燃油系统为例,验证了模... 船舶设备众多、型号多样、结构复杂,故障预测所需的有效数据偏少,而大规模的在线数据监测又受制于存储空间和计算时间的限制。利用On-line SVM建立了故障预测模型,分析了船舶设备故障预测的流程;并以某船舶主机的燃油系统为例,验证了模型的准确性,认为基于On-line SVM的故障预测精度较高,解决了船舶设备故障预测中的超大样本、非线性以及高维复杂数据问题。 展开更多
关键词 on-line SVM 船舶设备 故障预测 参数优化
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Multi-Sensor Intelligent System for On-Line and Real-Time Moneitoring Tool Cutting State in FMS 被引量:1
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作者 徐春广 王信义 +1 位作者 邢济收 杨大勇 《Journal of Beijing Institute of Technology》 EI CAS 1997年第3期258-266,共9页
The principle and the constitution of an intelligent system for on-line and real-time montitoring tool cutting state were discussed and a synthetic sensors schedule combined a new type fluid acoustic emission sens... The principle and the constitution of an intelligent system for on-line and real-time montitoring tool cutting state were discussed and a synthetic sensors schedule combined a new type fluid acoustic emission sensor (AE) with motor current sensor was presented. The parallel communication between control system of machine tools, the monitoring intelligent system,and several decision-making systems for identifying tool cutting state was established It can auto - matically select the sensor way ,monitoring mode and identifying method in machining process- ing so as to build a successful and effective intelligent system for on -line and real-time moni- toring cutting tool states in FMS. 展开更多
关键词 tool cutting state on-line monitoring intelligent system acoustic emission sensor
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On-line机行血液透析滤过两种稀释模式效果的临床观察 被引量:2
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作者 易铁钢 祁爱蓉 程淑碧 《中国血液净化》 2004年第1期41-42,47,共3页
关键词 on-line 血液透析滤过 稀释模式 HDF 治疗 血液净化
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On-line hemodiafiltration技术和临床应用 被引量:25
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作者 谢红浪 季大玺 《肾脏病与透析肾移植杂志》 CAS CSCD 2005年第4期377-381,共5页
关键词 血液净化 肾脏疾病 临床 on-line 临床应用 肾脏替代治疗 终末期肾脏疾病 血液透析滤过 常规血液透析 生物相容性
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Development and Application of On-Line Corrosion Monitoring Device for Condenser Tube 被引量:1
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作者 曹杰玉 宋敬霞 +2 位作者 汪德良 龙国军 孙本达 《Electricity》 2004年第2期31-35,共5页
This paper introduces the development and industrial application of an on-line corrosion monitoring device for condenser tubes. Corrosion sensors are made up of representative condenser tubes chosen by eddy current te... This paper introduces the development and industrial application of an on-line corrosion monitoring device for condenser tubes. Corrosion sensors are made up of representative condenser tubes chosen by eddy current test, which enable the monitoring result to be consistent with the corrosion of actual condenser tubes. Localized corrosion rate of condenser tubes can be measured indirectly by a galvanic couple made up of tube segments with and without pits. Using this technology, corrosion problems can be found in time and accurately, and anticorrosive measures be made more economic and effective. Applications in two power plants showed the corrosion measurements are fast and accurate. 展开更多
关键词 condenser tube CORROSION on-line monitoring INSTRUMENTATION
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平行机的实时到达on-line算法下界的改进 被引量:1
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作者 陈仕平 姚恩瑜 《高校应用数学学报(A辑)》 CSCD 北大核心 1999年第3期315-318,共4页
本文考虑了平行机实时到达的在线问题.模型中,工件是陆续到达的,工件的个数、到达时间是事先未知的,而且只有当工件到达,才知其加工时间,目标是使所有工件都加工完的时间达到最小.Chen与Vestjens(1996年)证明... 本文考虑了平行机实时到达的在线问题.模型中,工件是陆续到达的,工件的个数、到达时间是事先未知的,而且只有当工件到达,才知其加工时间,目标是使所有工件都加工完的时间达到最小.Chen与Vestjens(1996年)证明了该在线问题不存在性能比小于1.3473的on-line算法.本文将此界改进为(5-5)/2. 展开更多
关键词 排序 on-line算法 平行机 组合最优化 实时到达
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Effects of on-line solution and off-line heat treatment on microstructure and hardness of die-cast AZ91D alloy 被引量:1
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作者 徐玉磊 张奎 +3 位作者 李兴刚 雷健 袁海波 刘正 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第11期2652-2658,共7页
The effects of on-line solution, off-line solution and aging heat treatment on the microstructure and hardness of the die-cast AZ91D alloys were investigated. Brinell hardness of die-cast AZ91D alloy increases through... The effects of on-line solution, off-line solution and aging heat treatment on the microstructure and hardness of the die-cast AZ91D alloys were investigated. Brinell hardness of die-cast AZ91D alloy increases through on-line solution and off-line aging treatment but decreases after off-line solution treatment. By X-ray diffractometry, optical microscopy, differential thermal analysis, scanning electron microscopy and X-ray energy dispersive spectroscopy, it is found that the microstructures of the die-cast AZ91D magnesium alloy before and after on-line solution and off-line aging are similar, consisting of α-Mg and β-Al12Mg17. The precipitation of Al element is prevented by on-line solution so that the effect of solid solution strengthening is enhanced. The β-Al12Mg17 phases precipitate from supersaturated Mg solid solution after off-line aging treatment, and lead to microstructure refinement of AZ91D alloy, so the effect of precipitation hardening is enhanced. The β-Al12Mg17 phases dissolve in the substructure after off-line solution treatment, which leads to that the grain boundary strengthening phase is reduced significantly and the hardness of die cast AZ91D is reduced. 展开更多
关键词 die-cast magnesium alloy AZ91D alloy on-line solution off-line solution treatment aging treatment β-Al12Mg17 phases microstructure refinement precipitation hardening
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On-line血液透析滤过对维持性血液透析患者心率变异性的影响 被引量:5
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作者 侯丹丹 王淑领 尚赛男 《临床和实验医学杂志》 2017年第17期1761-1764,共4页
目的探讨On-line血液透析滤过技术对维持性血液透析患者心率变异性(HRV)的影响。方法 50例维持性血液透析(MHD)患者随机分为常规血液透析治疗组(HD组)和On-line血液透析滤过治疗组(OL-HDF组),每组各25例。患者每周行3次、每次4 h的血液... 目的探讨On-line血液透析滤过技术对维持性血液透析患者心率变异性(HRV)的影响。方法 50例维持性血液透析(MHD)患者随机分为常规血液透析治疗组(HD组)和On-line血液透析滤过治疗组(OL-HDF组),每组各25例。患者每周行3次、每次4 h的血液透析治疗或OL-HDF,治疗24个月。比较两组常规的实验室检查和24h动态心电图监测结果。结果治疗24个月后,OL-HDF组的β2-微球蛋白(β2-MG)由基线值30.1±7.2 mg/dl降至21.7±6.1 mg/dl(P<0.05),HD组的β2-MG则无明显下降。OL-HDF组HRV的时域性和频域性指标均值高于基线值(P<0.05),HD组的HRV指标则无显著性变化(P>0.05)。结论与普通血液透析比较,OL-HDF能更好地改善MHD患者的HRV,改善自主神经功能。 展开更多
关键词 维持性血液透析 on-line血液透析滤过心率变异性
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On-line置换液与生理盐水在血液透析滤过回血中的应用效果比较 被引量:5
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作者 祝喜鹰 刘金凤 肖静 《护理研究(上旬版)》 2014年第7期2370-2372,共3页
[目的]探讨On-line置换液与生理盐水在血液透析滤过(HDF)病人回血中的应用效果,以期为临床护理工作提供理论依据。[方法]选取30例规律HDF病人为研究对象,采用自身对照方法,每例病人每周依次采用On-line置换液和生理盐水回血,比较回血过... [目的]探讨On-line置换液与生理盐水在血液透析滤过(HDF)病人回血中的应用效果,以期为临床护理工作提供理论依据。[方法]选取30例规律HDF病人为研究对象,采用自身对照方法,每例病人每周依次采用On-line置换液和生理盐水回血,比较回血过程中残余血量、静脉壶液体温度、护士的工作量及经济效益。[结果]采用On-line置换液回血法残余血量及回血后静脉壶温度分别为(1.89±0.91)mL,(33.36±0.15)℃,而采用生理盐水法为(2.88±0.23)mL、(27.13±0.14)℃,差异均有统计学意义(P<0.01);而透析器凝血状况比较差异无统计学意义(P=0.08);采用On-line置换液回血时护士工作量减少,回血液体成本降低。[结论]应用接近生理温度的On-line置换液回血能稀释血液、降低血液黏稠度,减少血路管残余血量,增加病人舒适感,提高工作效率。 展开更多
关键词 联机血液透析滤过 液体回血 on-line置换液 生理盐水 残余血量 工作量
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On-line HPLC-DAD coupled with ESI-IT-TOF-MS and fluorescence detection to identify DNA-binding compounds from Lithospermum erythrorhizon using acridine orange as the fluorescence probe
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作者 黄文芳 张藏蔓 +3 位作者 李森森 梁毅 王弘 陈世忠 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2015年第9期581-590,共10页
We have developed an on-line detection method using acridine orange as the fluorescence probe and applied this method to rapidly identify active compounds in herbal medicines. This on-line method was equipped with a h... We have developed an on-line detection method using acridine orange as the fluorescence probe and applied this method to rapidly identify active compounds in herbal medicines. This on-line method was equipped with a high-performance liquid chromatography tandem diode array detector, electrospray ionization-ion-trap time-of-flight mass spectrometry and DNA- acridine orange fluorescence detection (HPLC-DAD-MSn-DNA-AO-FLD). A large amount of information could be simultaneously obtained during one run, which included HPLC fingerprint, ultraviolet spectra, total ion chromatograms, MSn data of high-resolution mass spectrometry and activity profile of each compound binding with DNA. The method also provided information on structureactivity relationships and mechanism of interaction. We used this on-line method to identify five DNA-binding activity components from Lithospermum erythrorhizon sample for the first time. The result showed that the parent nucleus of shikonin derivatives could bind with DNA. The structure-activity relationship showed that the parent nucleus of shikonin derivatives plays a major role in DNA binding, not the carboxyl group on the side chain. This simple, rapid, high precision and good stability on-line method should be useful for compound separation, structural identification and screening of DNA-binding compounds in herbal medicines. 展开更多
关键词 on-line Lithospermum erythrorhizon Acridine orange DNA DNA-binding activity
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On-lineHDF的临床观察及护理 被引量:1
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作者 李虹 张永勤 汪吉平 《实用全科医学》 2004年第3期280-281,共2页
目的 探讨应用On -lineHDF治疗慢性肾衰尿毒症患者的临床效果及护理对策。方法 回顾性分析 15例维持性HD患者应用On -lineHDF的治疗及护理过程。结果  15例患者经每 1~ 2周 1次的On -lineHDF治疗后 ,病情稳定 ,部分患者心功能、血... 目的 探讨应用On -lineHDF治疗慢性肾衰尿毒症患者的临床效果及护理对策。方法 回顾性分析 15例维持性HD患者应用On -lineHDF的治疗及护理过程。结果  15例患者经每 1~ 2周 1次的On -lineHDF治疗后 ,病情稳定 ,部分患者心功能、血压、食欲、体力及睡眠不同成度得到改善 ,在促红细胞生成素使用量不变的情况下 ,4例病人的血红蛋白有所增加。结论 On -lineHDF是当今最佳的肾脏替代疗法技术之一。 展开更多
关键词 on-line HDF 护理措施 血液透析 置换液 治疗
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