Omsk hemorrhagic fever virus(OHFV) is a tick-borne flavivirus classified as a biosafety level-4(BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccine...Omsk hemorrhagic fever virus(OHFV) is a tick-borne flavivirus classified as a biosafety level-4(BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion(OHFVDNS1) and reporter virus replacing NS1 with the Gaussia luciferase(Gluc)(OHFV-ΔNS1-Gluc). Both the defective OHFVDNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1(BHK21NS1) but not in na?ve BHK21 cells. The Gluc reporter gene of OHFV-ΔNS1-Gluc virus was maintained stably after serial passaging of BHK21NS1 cells and was used to surrogate the replication of OHFV. Using NITD008, OHFV-ΔNS1-Gluc virus was validated for antiviral screening, and high-throughput screening parameters were optimized in a 96-well plate format with a calculated Z0 value above 0.5. The OHFV-ΔNS1-Gluc reporter virus is a powerful tool for antiviral screening as well as viral replication and pathogenesis studies in BSL2 laboratories.展开更多
Omsk hemorrhagic fever virus(OHFV)is a tick-borne flavivirus associated with acute febrile illness and hemorrhagic disease in humans.OHFV was first identified in 1947 and since its identification has largely remained ...Omsk hemorrhagic fever virus(OHFV)is a tick-borne flavivirus associated with acute febrile illness and hemorrhagic disease in humans.OHFV was first identified in 1947 and since its identification has largely remained endemic to the Omsk region of the Russian Federation,where it continues to result in illnesses each year.However,the 2 primary tick vectors of OHFV exhibit ranges that extend far beyond the Omsk region,and the recent positive confirmation of OHFV in samples outside of the historic endemic range,in both the Russian Federation and neighboring Kazakhstan,to the south raises new concerns about the future potential of the pathogen.In this review,we comprehensively discuss the emergence,virology,ecology,and disease of OHFV.展开更多
Located in central China on the bank of the Yellow River,Kaifeng is well-known for its rich history and culture and its position as the country’s capital in eight ancient dynasties.The famous scroll painting titled R...Located in central China on the bank of the Yellow River,Kaifeng is well-known for its rich history and culture and its position as the country’s capital in eight ancient dynasties.The famous scroll painting titled Riverside Scenes at Qingming Festival depicts the city’s prosperity and patronage by travelers and merchants from both home and abroad.Today,Kaifeng has quickened its exchanges and cooperation with the outside world,banking on the opportunities brought by the Chinese government’s decisions to integrate the development of Zhengzhou and Kaifeng展开更多
基金supported by National Science and Technology Major Project on Important Infectious Diseases Prevention and Control (2018ZX10734404-010)National Key Research and Development Program of China (2018YFA0507201)
文摘Omsk hemorrhagic fever virus(OHFV) is a tick-borne flavivirus classified as a biosafety level-4(BSL4) pathogen. Studies of OHFV are restricted to be conducted within BSL4 laboratories. Currently, no commercial vaccines or antiviral drugs are available against OHFV infection. In this study, we recovered a replication-deficient OHFV with an NS1 deletion(OHFVDNS1) and reporter virus replacing NS1 with the Gaussia luciferase(Gluc)(OHFV-ΔNS1-Gluc). Both the defective OHFVDNS1 and OHFV-ΔNS1-Gluc virus could only replicate efficiently in the BHK21 cell line expressing NS1(BHK21NS1) but not in na?ve BHK21 cells. The Gluc reporter gene of OHFV-ΔNS1-Gluc virus was maintained stably after serial passaging of BHK21NS1 cells and was used to surrogate the replication of OHFV. Using NITD008, OHFV-ΔNS1-Gluc virus was validated for antiviral screening, and high-throughput screening parameters were optimized in a 96-well plate format with a calculated Z0 value above 0.5. The OHFV-ΔNS1-Gluc reporter virus is a powerful tool for antiviral screening as well as viral replication and pathogenesis studies in BSL2 laboratories.
基金supported by a Holcomb Awards Committee grant(028611)through Butler Uniersity for Christopher C.Stobart.
文摘Omsk hemorrhagic fever virus(OHFV)is a tick-borne flavivirus associated with acute febrile illness and hemorrhagic disease in humans.OHFV was first identified in 1947 and since its identification has largely remained endemic to the Omsk region of the Russian Federation,where it continues to result in illnesses each year.However,the 2 primary tick vectors of OHFV exhibit ranges that extend far beyond the Omsk region,and the recent positive confirmation of OHFV in samples outside of the historic endemic range,in both the Russian Federation and neighboring Kazakhstan,to the south raises new concerns about the future potential of the pathogen.In this review,we comprehensively discuss the emergence,virology,ecology,and disease of OHFV.
文摘Located in central China on the bank of the Yellow River,Kaifeng is well-known for its rich history and culture and its position as the country’s capital in eight ancient dynasties.The famous scroll painting titled Riverside Scenes at Qingming Festival depicts the city’s prosperity and patronage by travelers and merchants from both home and abroad.Today,Kaifeng has quickened its exchanges and cooperation with the outside world,banking on the opportunities brought by the Chinese government’s decisions to integrate the development of Zhengzhou and Kaifeng
