[Objective] Ogura cytoplasmic male sterility （Ogu CMS） is an ideal pollina- tion control system for heterosis utilization in Brassica napus. However, fertility-restor- ing （Rf） gene only exists in radish chromosom...[Objective] Ogura cytoplasmic male sterility （Ogu CMS） is an ideal pollina- tion control system for heterosis utilization in Brassica napus. However, fertility-restor- ing （Rf） gene only exists in radish chromosome and is closely linked with glucosino- late gene, making it hardly to be applied directly in production. Thus, the key to apply Ogu CMS in Brassica napus is to introduce the Rf genes and to break its linkage with glucosinolate gene. [Me^od] To overcome the interspecific reproductive barrier, grafting was conducted by using Raphanobrassica （2n=-58） as donor materi- als of Rf genes. The obtained interspecific hybrids were analyzed from the agronom- ic traits, seed-setting rate and fertility restoration rate for screening fertility-restoring materials. [Results] By elaborative selection, a homozygous Ogu CMS fertility-restor- ing B. napus material named CLR650 was selected out, whose somatic chromosome numbers were found to be 2n=38-40. Some abnormal phenomena like anaphase bridges and lagging chromosomes in meiosis were observed, but the abnormalities did not affect the formation of normal pollens. It can restore the male fertility in both progenies of self-pollination and testcross by 100%. Molecular analysis showed that CLR650 harbors Ogu CMS fertility-restoring gene, which is obviously different from that of Ogu CMS restore line Rl13 and RHH1 by detecting the molecular markers closely linked with radish restorer gene （Rf0）. [Conclusion] The CLR650 could be a new restorer for the Ogu CMS in B. napus.展开更多
基金Supported by Open Fund of State Key Laboratory of Crop Genetic Improvement (ZK201101)the National High-Tech Research and Development Program of China(863 Program)(2009AA101105)Supported by the earmarked fund for Modern Agro-industry Technology Research System (CARS-13)~~
文摘[Objective] Ogura cytoplasmic male sterility （Ogu CMS） is an ideal pollina- tion control system for heterosis utilization in Brassica napus. However, fertility-restor- ing （Rf） gene only exists in radish chromosome and is closely linked with glucosino- late gene, making it hardly to be applied directly in production. Thus, the key to apply Ogu CMS in Brassica napus is to introduce the Rf genes and to break its linkage with glucosinolate gene. [Me^od] To overcome the interspecific reproductive barrier, grafting was conducted by using Raphanobrassica （2n=-58） as donor materi- als of Rf genes. The obtained interspecific hybrids were analyzed from the agronom- ic traits, seed-setting rate and fertility restoration rate for screening fertility-restoring materials. [Results] By elaborative selection, a homozygous Ogu CMS fertility-restor- ing B. napus material named CLR650 was selected out, whose somatic chromosome numbers were found to be 2n=38-40. Some abnormal phenomena like anaphase bridges and lagging chromosomes in meiosis were observed, but the abnormalities did not affect the formation of normal pollens. It can restore the male fertility in both progenies of self-pollination and testcross by 100%. Molecular analysis showed that CLR650 harbors Ogu CMS fertility-restoring gene, which is obviously different from that of Ogu CMS restore line Rl13 and RHH1 by detecting the molecular markers closely linked with radish restorer gene （Rf0）. [Conclusion] The CLR650 could be a new restorer for the Ogu CMS in B. napus.
