The use of recycled concrete and oyster shells as partial cement and aggregate replacements is ongoing research to solve this multifaceted problem of concrete waste in the construction industry as well as waste from o...The use of recycled concrete and oyster shells as partial cement and aggregate replacements is ongoing research to solve this multifaceted problem of concrete waste in the construction industry as well as waste from oyster shell farming. However, there is a lack of evidence on the possibility of producing a fully recycled composite consisting of recycled concrete and oyster shell without the need for new cement and natural aggregates. In this study, recycled concrete powder (RCP) and oyster shell were used to produce a green composite. Separate ground and combined ground (separate ground and co-ground) RCP and oyster shells are used to determine the effects of grinding approaches on the mechanical and chemical properties of the composite. The composite samples were molded via press molding by applying 30 MPa of pressure for 10 minutes. The results revealed that the composite prepared via the combined ground approach presented the highest flexural strength compared to the separate ground and unground samples. The FTIR and XRD characterization results revealed no chemical or phase alterations in the raw materials or the resulting composites before and after grinding. SEM analysis revealed that combined grinding reduced the particles’ size and improved the dispersion of the mixture, thereby increasing the strength.展开更多
The restoration of European flat oyster (Ostrea edulis) population is critical for biodiversity and ecosystem services recovery. This study evaluates the potential of innovative clay substrates, developed and patented...The restoration of European flat oyster (Ostrea edulis) population is critical for biodiversity and ecosystem services recovery. This study evaluates the potential of innovative clay substrates, developed and patented by Oyster Heaven, as cost-effective and degradable alternatives for large-scale oyster reef restoration. Field trials conducted in an oyster aquaculture site and hatchery experiments under controlled conditions assessed the larvae settlement efficiency, microbial biofilm composition, and environmental influences. Results showed significantly lower larvae recruitment on clay prototypes and spat collectors used as control, in wild conditions, with environmental factors such as temperature and hypoxia playing a key role. In the hatchery, larvae preferred oyster shells (17,174 ± 659 spats/m2) over clay prototypes (2917 ± 111 spats/m2) and spat collectors (1451 ± 160 spats/m2). Microbial analysis revealed a shift towards Cyanobacteria and Woeseia species, with Pseudoalteromonadaceae, a genus previously linked to both stimulating and inhibiting larvae settlement, detected only on clay surfaces. Despite challenges, the clay prototypes demonstrated potential as sustainable substrates for restoration, particularly in managed “spats-on-reefs” strategies. This research highlights the importance of environmental monitoring, microbial interactions, and substrate optimization in enhancing restoration success for O. edulis populations. Further investigations are recommended to refine clay designs and assess their broader applicability under variable environmental conditions.展开更多
The increasing demand for renewable energy sources has driven the exploration of innovative materials for biofuel production.This study investigates bio-pellet characteristics derived from several oyster mushroom bagl...The increasing demand for renewable energy sources has driven the exploration of innovative materials for biofuel production.This study investigates bio-pellet characteristics derived from several oyster mushroom baglog wastes with varying concentrations of molasses as an adhesive.The process began with sun-drying the baglog waste for three days,followed by oven drying at 80○C for 24 h.Bio-pellets were produced by blending baglog waste with molasses at concentrations of 5%and 10%(w/v),then subsequently fed into a pellet mill.The bio-pellets were left to rest for one hour before analysis.The quality of bio-pellets was determined by evaluating moisture content,ash content,volatile matter,calorific value,combustion rate,density,and compressive strength following SNI 8675:2018 standards.Results indicate that adding molasses as a binder significantly affected the bio-pellet quality.The optimal molasses concentration for balanced performance was found at 5%,providing a lowermoisture content(6.8%),volatile matter(68.42%),and density(1.55 g⋅cm−3).In addition,the bio-pellet has a slightly higher calorific value(approximately 3614 cal⋅g−1),compressive strength(40.68 N⋅mm−2),and ash content(18.59%).All of the parameters for the bio-pellet containing 5%molasses satisfied the standard except for ash content and calorific value.展开更多
Aceh in Indonesia is rich inmarine resources and abundant fishery products such as oyster.Traditionally,fishermen only harvest oysters and discard the shells,which can cause pollution and environmental contamination.W...Aceh in Indonesia is rich inmarine resources and abundant fishery products such as oyster.Traditionally,fishermen only harvest oysters and discard the shells,which can cause pollution and environmental contamination.Waste Oyster Shells(WOS)contain a high percentage of calcium carbonate(CaCO_(3))that experiences thermal decomposition at high temperature,following the reaction CaCO_(3)→CaO+CO_(2)(ΔT=825℃).At temperature>900℃,dead-burned lime is formed,which severely influences CaO reactivity.However,the optimum temperature for producing high CaO content is still uncertain.Therefore,this study aimed to determine the optimum calcination temperature to produce high CaO content,assess initial setting time of WOS paste,and identify the best compressive strength of paste.For the experiment,WOS was used as a partial cement replacement(with a size of 0.075 mm)in paste at a proportion of 5%and calcined at temperature of 700℃,800℃,900℃,and 1000℃.The specimens used were an ebonite ring(dimensions:70 mm bottom diameter,60 mm top diameter,and 40 mm height)and a cube(dimensions:5 cm×5 cm×5 cm).The experiment was conducted following the ASTM(American Society for Testing andMaterials)standards and optimumcompressive strength values were analyzed using ANOVA(Analysis of Variance)and Response Surface Methodology(RSM)through the Design Expert software.The results showed that WOS calcined at 1000℃ increased CaO content by approximately 57.40%.Furthermore,the initial setting time test of 5%WOS paste at 1000℃ showed a more uniform binding performance compared to conventional cement paste,with an initial setting time of 75 min and a penetration depth of 15 mm.In line with the analysis,optimum compressive strength of 71.028 MPa with a desirability value of 0.986 was achieved at 5%cement replacement and calcination temperature of 786.44℃.展开更多
Oyster farming provides substantial ecological and economic benefits but is often constrained by the challenges of selecting suitable sites in dynamic coastal environments.This study presents a tailored oyster suitabi...Oyster farming provides substantial ecological and economic benefits but is often constrained by the challenges of selecting suitable sites in dynamic coastal environments.This study presents a tailored oyster suitability index(OSI)for the Zhujiang(Pearl)River Estuary(PRE),developed using Landsat satellite imagery and in situ observations collected from 2013 to 2023.Key environmental parameters,including sea surface temperature(SST),salinity,turbidity,and chlorophyll-a(Chl-a)concentration,were integrated for OSI retrieval.Optimal algorithms for each parameter were identified through evaluation using field measurements,yielding high accuracy,as evidenced by strong determination coefficients(R^(2))and low root mean square error(RMSE):R^(2)=0.98,RMSE=0.74℃for SST;R^(2)=0.94,RMSE=0.50 for salinity;R^(2)=0.95,RMSE=1.21 mg/m^(3)for Chl-a;R^(2)=0.91,RMSE=1.48 NTU for turbidity.The OSI revealed pronounced seasonal and spatial variability,with the highest suitability observed during winter and the lowest during summer.Validation results demonstrated strong alignment between OSI predictions and existing oyster farming zones.These findings underscore the value of remote sensing for scalable,near-real-time aquaculture site assessments.The OSI framework provides a robust decision-support tool for optimizing oyster cultivation,promoting sustainable aquaculture development in dynamic estuarine systems such as the PRE and beyond.展开更多
This study explores the relationship between cardiac activity and biochemical indicators in Pacific oysters(Crassostrea gigas)during cold storage to develop a nondestructive vitality assessment method.Oysters were sto...This study explores the relationship between cardiac activity and biochemical indicators in Pacific oysters(Crassostrea gigas)during cold storage to develop a nondestructive vitality assessment method.Oysters were stored at−1℃ for 14 d,with cardiac patterns tracked via image analysis,and biochemical markers(pH,adenosine triphosphate(ATP)-related compounds,and adenylate energy charge(AEC))were assessed.Five cardiac patterns were identified,with regular alternating contractions common early but decreasing over time,aligning with declines in AEC(44.11%-35.52%)and pH(6.98-6.55).The intervals between ventricular and atrial contractions rose from 4.2 to 5.6 s,strongly correlating with biochemical signs of vitality loss.Image analysis revealed characteristic waveforms for each cardiac pattern,despite amplitude variations caused by optical artifacts.These findings indicate that cardiac pattern analysis via image processing could be an effective nondestructive indicator of oyster vitality,offering a novel approach to quality control in shellfish storage and distribution.展开更多
Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors...Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors for food resource with the cultured species. Fouling organisms may also reduce the oxygen supply. A study was conducted to measure the impact of foulers on feeding oxygen consumption, and waste excretion. POM, ammonia, phosphate and oxygen concentration were measured in various treatments （cultured species, foulers）. This study showed that fouling organisms had significant effect on food uptake, oxygen consumption and waste excretion. The clearance rate, ammonia and phosphate release rate, oxygen consumption rate of the fouled pearl oyster were greater than those of the clean pearl oysters. Other foulers that settled on cages or buoys also contributed much to phytoplankton depletion, oxygen consumption and concentration increase of ammonia and phosphate in water. Therefore, this study showed us that foulers were important competitors in the pearl oyster cultivation of Daya Bay in November 2005.展开更多
Oyster cultivation is a pivotal economic industry in Hainan Island,South China,where a high oyster species diversity is shown.However,the specific distribution and biodiversity of oyster resources in the island have r...Oyster cultivation is a pivotal economic industry in Hainan Island,South China,where a high oyster species diversity is shown.However,the specific distribution and biodiversity of oyster resources in the island have remained unclear.To elucidate the diversity of oyster species and their distribution there,307 oyster samples were collected from 29 sites in the intertidal zone around the island,and were identified using both morphological and molecular approaches.A minimum of 12 oyster species were identified in taxonomy,including Crassostrea species(C.gigas angulata,C.sikamea,C.iredalei,C.dianbaiensis,C.talonata,C.ariakensis,and C.hongkongensis),and Saccostrea species(S.malabonensis,S.mordax,S.echinata,S.circumsuta,and S.mordoides).The results revealed a remarkable diversity of oyster species along the coast of the island.Particularly noteworthy are that S.malabonensis and S.mordax constituted 36%and 22%of the collected specimens,respectively.This study provided a comprehensive overview on current state of oyster biodiversity in Hainan,serving as a valuable reference for conservation and research on species distribution and resource dynamics in oyster populations.展开更多
Concentrations of 8 heavy metals: cadmium (Cd), copper (Cu), zinc (Zn), lead (Pb), nickel (Ni), chromium (Cr), antimony (Sb) and tin (Sn) were examined in 3 species of bivalves (Perna viridis, Crassostrea rivularis ...Concentrations of 8 heavy metals: cadmium (Cd), copper (Cu), zinc (Zn), lead (Pb), nickel (Ni), chromium (Cr), antimony (Sb) and tin (Sn) were examined in 3 species of bivalves (Perna viridis, Crassostrea rivularis and Ruditapes philippinarum) collected from 25 sites along the Pearl River Delta coastal waters in the South China Sea from July to August 1996. In general, Cd, Cu, Zn and Sn concentrations in the three bivalve species collected from the Estuarine Zone were significantly higher than those collected from the Western and Eastern Zones of the Pearl River Delta, which are related to the existence of various anthropogenic activities in the catchment of the Pearl River Delta. The Western Estuarine Zone is mainly impacted by Cr, Ni and Cu contamination. In Victoria Harbor, heavy metal contamination is mainly due to Cu and Pb. Cd, Cu and Zn concentrations in oysters were significantly higher than those in mussels and clams. This could be explained by the fact that oysters live mainly in the Estuarine Zone of the Pearl River Delta which receives most of the polluting discharges from the catchment of the Delta. During turbid condition, heavy metals(soluble or adsorbed on suspended particulates) discharged from the Delta are filtered from the water column and subsequently accumulated into the soft body tissues of oysters. Heavy metal concentrations in the three bivalve species were compared with the maximum permissible levels of heavy metals in seafood regulated by the Public Health and Municipal Services Ordinance, Laws of Hong Kong, and it was revealed that Cd and Cr concentrations in the three bivalve species exceeded the upper limits. At certain hotspots in the Delta, the maximum acceptable daily load for Cd was also exceeded.展开更多
The incidence of diabetes has increased considerably, and become the third serious chronic disease following cancer and cardiovascular diseases. Though acarbose, metformin, and 1-deoxynojirimycin have good efficacy fo...The incidence of diabetes has increased considerably, and become the third serious chronic disease following cancer and cardiovascular diseases. Though acarbose, metformin, and 1-deoxynojirimycin have good efficacy for clinical application as hypoglycemic drugs, their expensive costs and some degree of side effects have limited their clinical application. Recently, increasing attention has concentrated on the polysaccharides from natural plant and animal sources for diabetes. In order to illustrate the pharmaceutical activity of polysaccharides as natural hypoglycemic agents, polysaccharides isolated from Astragalus, oyster mushroom, and Yacon were evaluated for their inhibitory effects on α-glucosidase. Polysaccharides were extracted and purified from Astragalus, Oyster mushroom, and Yacon with hot water at 90 °C for 3 h, respectively. The total sugar content of the polysaccharide was determined by the phenol-sulfuric acid method. The α-glucosidase inhibitory activity was measured by the glucose oxidase method. The results exhibited that the inhibitory effects on α-glucosidase were in decreasing order, Astragalus > oyster mushroom > Yacon. The α-glucosidase inhibition percentage of Astragalus polysaccharide and oyster mushroom polysaccharide were over 40% at the polysaccharide concentration of 0.4 mg·mL-1. The IC50 of Astragalus polysaccharide and oyster mushroom polysaccharide were 0.28 and 0.424 mg·mL-1, respectively. The information obtained from this work is beneficial for the use polysaccharides as a dietary supplement for health foods and therapeutics for diabetes.展开更多
Estuaries have been described as one of the most difficult environments on Earth. It is difficult to know how to treat the combined wastewater in tidal rivers at the estuary, where the situation is very different from...Estuaries have been described as one of the most difficult environments on Earth. It is difficult to know how to treat the combined wastewater in tidal rivers at the estuary, where the situation is very different from ordinary fresh water rivers. Waste oyster shell was used as the active filler in this study in a bio-contact oxidation tank to treat the combined wastewater at the Fengtang Tidal River. With a middle-experimental scale of 360 ma/day, the average removal efficiency of COD, BOD, NH3-N, TP and TSS was 80.05%, 85.02%, 86.59%, 50.58% and 85.32%, respectively, in this bio-contact oxidation process. The living microbes in the biofilms on the waste oyster shell in this bio-contact oxidation tank, which were mainly composed of zoogloea, protozoa and micro-metazoa species, revealed that waste oyster shell as the filler was suitable material for combined wastewater degradation. This treatment method using waste oyster shell as active filler was then applied in a mangrove demonstration area for water quality improvement near the experiment area, with a treatment volume of 5 × 10^3 m^3/day. Another project was also successfully applied in a constructed wetland, with a wastewater treatment volume of 1 ×10^3 m^3/day. This technology is therefore feasible and can easily be applied on a larger scale,展开更多
In this study, Pearl oyster mantle type V collagen(POMC) and tilapia scale type I collagen(TSC) were extracted and hydrolyzed by various proteases in order to obtain peptides. The antioxidant activity of the peptides ...In this study, Pearl oyster mantle type V collagen(POMC) and tilapia scale type I collagen(TSC) were extracted and hydrolyzed by various proteases in order to obtain peptides. The antioxidant activity of the peptides was investigated by DPPH, hydroxyl radical scavenging experiments and a dynamic digestion model in vitro. The results show that there are significant differences in amino acid composition between POMC and TSC. The collagen peptides obtained from pearl oyster mantle(POMCP) by treating with alkaline protease exhibited higher antioxidant activity than that from tilapia scale(TSCP) treated with papaya protease, and both of them showed greater DPPH and hydroxyl radical scavenging activity than other peptides. After being separated via Sephadex G-25 chromatography, the M1 fraction isolated from POMCP, and the S1 fraction from TSCP with which both had higher molecular weights showed the strongest antioxidant activity than other fractions, and the M1 fraction exhibited stronger antioxidant activity than the S1 fraction in scavenging free-radicals and protecting cells from the oxidation damage. Furthermore, after treating the dynamic digestion system model in vitro, the DPPH and hydroxyl radical scavenging activity of the M1 fraction increased slightly. These results suggest that POMCP exhibits stronger antioxidant activity than TSCP, which means that PMOP may be a good candidate to be a potential natural antioxidant in the food-processing industry.展开更多
Oyster peptide zinc nanoparticles (OPZNPs)(28 108 nm) were prepared in the presence of 0.5% 0.9% zinc sulfate at pH 6.0 11.0. The obtained nanoparticles exhibited uniform size distribution and spherical shapes. Nanopa...Oyster peptide zinc nanoparticles (OPZNPs)(28 108 nm) were prepared in the presence of 0.5% 0.9% zinc sulfate at pH 6.0 11.0. The obtained nanoparticles exhibited uniform size distribution and spherical shapes. Nanoparticle characteristics, such as size, surface charge, and hydrophobicity, could be adjusted by controlling zinc sulfate concentration and environmental pH. In- creasing pH value or decreasing zinc sulfate concentration tended to reduce nanoparticle size and increase nanoparticle surface charge and hydrophobicity. OPZNPs presented good stability at near-neutral pH and could be stored for at least 20 days at 4℃. The results of the peptide conformation study and nanoparticle dissociation test proved that zinc ions and carboxyl groups are the key factors that affect OPZNP formation. The intermolecular combinations of carboxyl groups via zinc bridging facilitated the aggrega- tion of oyster peptides. Nanoparticle formation was accompanied by aggregate association and conformational changes. These changes included increments in β-sheets, especially intermolecular β-sheets, at the expense of α-helixes. Overall, this work provided a green alternative route for the synthesis of OPZNPs.展开更多
The changes in the contents of adenosine 5’-triphosphate (ATP) and its related breakdown compounds were investigated in the adductor muscle, mantle, gill and body trunk of oyster (Crassostrea gigas) during frozen sto...The changes in the contents of adenosine 5’-triphosphate (ATP) and its related breakdown compounds were investigated in the adductor muscle, mantle, gill and body trunk of oyster (Crassostrea gigas) during frozen storage at -20℃ and -30℃ and compared with that of the fresh oyster. The investigation was performed using an HPLC system. Different extents of ATP decomposi- tion were found in various tissues frozen at the two temperatures. The K, K’ and A.E.C values were calculated as the chemical fresh- ness indices. Considering the results of sensory evaluation, the A.E.C. value in body trunk at -20℃ and -30℃ could be used as the best freshness index for frozen oyster.展开更多
The effects of high pressure(HP) treatment on spoilage characteristic and shelf life extension of Pacific oysters(Crassostrea gigas) during refrigerated storage were studied.Results showed that HP treatment of 275 MPa...The effects of high pressure(HP) treatment on spoilage characteristic and shelf life extension of Pacific oysters(Crassostrea gigas) during refrigerated storage were studied.Results showed that HP treatment of 275 MPa for 3 min or 300 MPa for 2 min could achieve 100% full release of oyster adductor muscle,pressures higher than 350 MPa caused excessive release as the shells of oysters were broken,thus use of higher pressures should be cautious in oyster processing industry because of its adverse impact on the appearance of shells.HP treatment(300 MPa,2 min) was proper for the shucking of Pacific oyster(Crassostrea gigas) in China.This treatment caused no organoleptic disadvantage.Moreover,HP treatment resulted in obvious differences in biochemical spoilage indicators(p H,TVB-N and TBARS) changes and volatile compounds profile determined by electronic nose during storage.HP treatment(300 MPa,2 min) also led to a reduction of aerobic bacterial count(APC) by 1.27 log cycles.Furthermore,the APC values of oysters treated by HP were always lower than those of the control samples during storage.Based on the organoleptic,biochemical and microbiological indicators,shelf life of 6-8 d for control and 12 d for HP-treated oysters could be expected.HP treatment showed great potential in oyster processing and preservation.展开更多
Oyster reefs have an equivalent, complex 3-dimensional structure to vegetated habitats and may provide similar functions in estuarine environments. Nevertheless, few studies have compared oyster reefs with adjacent na...Oyster reefs have an equivalent, complex 3-dimensional structure to vegetated habitats and may provide similar functions in estuarine environments. Nevertheless, few studies have compared oyster reefs with adjacent natural shallow-water habitats. Here the resident benthic macroinvertebrate communities in an artificial oyster （Crassostrea ariakensis） reef and in adjacent natural estuarine shallow-water habitats （salt marsh, intertidal mudflat, and subtidal soft bottom） in the Changjiang （Yangtze） River estuary were described. The mean total densities and biomass, Margalef＇s species richness, Pielou＇s evenness and Shannon-Weaver biodiversity indices of the resident benthic macroinvertebrate communities differed significantly among the habitats. Significantly higher densities and biomass of benthic macroinvertebrates occurred in the oyster reef compared with the other three habitats. Ordination plots showed a clear separation in benthic macroinvertebrate communities among the four habitat types. The results demonstrated that the artificial oyster reef supported distinct and unique benthic communities, playing an important role in the complex estuarine habitat by supplying prey resources and contributing to biodiversity. In addition, the results suggested that the oyster reef had been restored successfully.展开更多
Based on the analysis of adsorptive features of oyster shells,the researches on the treatment of phosphorous wastewater with oyster shells and the effect of temperature on phosphorus removal were carried out.XRD was u...Based on the analysis of adsorptive features of oyster shells,the researches on the treatment of phosphorous wastewater with oyster shells and the effect of temperature on phosphorus removal were carried out.XRD was used to characterize the crystalline phases,and the main component of oyster shells was shown to be CaCO3.When the pretreatment temperature reached 800 ℃,some CaCO3 decomposed into CaO.As the temperature was further raised,CaO increased gradually.Via SEM testing,the oyster shell was a kind of natural porous materials.The pore wall partially collapsed from 550 to 900 ℃.No obvious porous structure was found at 900 ℃.However,without preheating,the oyster shell phosphorous removal material can not adsorb the phosphorus.Pretreatment made calcium activate,thus greatly increasing the absorption of phosphorus.展开更多
A light purple organic active substance capable of scavenging hydroxyl radical（OH） with a high efficiency was extracted from Oyster shell at an extraction rate of 2.49%. It was found for the first time that this act...A light purple organic active substance capable of scavenging hydroxyl radical（OH） with a high efficiency was extracted from Oyster shell at an extraction rate of 2.49%. It was found for the first time that this active substance may scavenge OH with the efficiency far higher than that of vitamin C. This active substance may scavenge also superoxide radical（O2） although the scavenging efficiency is far lower than that of vitamin C. Infrared spectrometry and routine chemical analysis primarily reveal that this active substance belongs to glycoprotein.展开更多
A two-dimensional numerical Computational Fluid Dynamics(CFD)model is established on the basis of viscous CFD theory to investigate the motion response and power absorption performance of a bottom-hinged flap-type wav...A two-dimensional numerical Computational Fluid Dynamics(CFD)model is established on the basis of viscous CFD theory to investigate the motion response and power absorption performance of a bottom-hinged flap-type wave energy converter(WEC)under regular wave conditions.The convergence study of mesh size and time step is performed to ensure that wave height and motion response are sufficiently accurate.Wave height results reveal that the attenuation of wave height along the wave tank is less than 5%only if the suitable mesh size and time step are selected.The model proposed in this work is verified against published experimental and numerical models.The effects of mechanical damping,wave height,wave frequency,and water depth on the motion response,power generation,and energy conversion efficiency of the flap-type WEC are investigated.The selection of the appropriate mechanical damping of the WEC is crucial for the optimal extraction of wave power.The optimal mechanical damping can be readily predicted by using potential flow theory.It can then be verified by applying CFD numerical results.In addition,the motion response and the energy conversion efficiency of the WEC decrease as the incident wave height increases because the strengthened nonlinear effect of waves intensifies energy loss.Moreover,the energy conversion efficiency of theWEC decreases with increasing water depth and remains constant as the water depth reaches a critical value.Therefore,the selection of the optimal parameters during the design process is necessary to ensure that the WEC exhibits the maximum energy conversion efficiency.展开更多
The Pacific oyster,Crassostrea gigas,naturally distributing along the coast of northwest Pacific,is one of the most important bivalve species due to its high economic value and fecundity.In China,we have initiated a s...The Pacific oyster,Crassostrea gigas,naturally distributing along the coast of northwest Pacific,is one of the most important bivalve species due to its high economic value and fecundity.In China,we have initiated a selective breeding program on both shell color and growth rate of C.gigas since 2010.A black shell line was obtained through four-generation family selection.In this study,mass selection for growth improvement was conducted in the sixth generation and seventh generation of black shell lines.To assess the progress of potential genetic improvement,the progeny of two generations of black shell lines were selected to evaluate their shell heights via a 450-day farming experiment.As the results,after growing for 450 days,the sixth generation and seventh generation of selected lines were 9.03% and 11.42% larger than the control lines,respectively.During the grow-out stage,the genetic gain of two generations was 8.82%±0.18% and 11.54%±0.43%,respectively;and the corresponding realized heritability was 0.45±0.04 and 0.41±0.04,respectively.These results indicated that the mass selection for shell height achieved steady progress in the two generations of C.gigas.展开更多
文摘The use of recycled concrete and oyster shells as partial cement and aggregate replacements is ongoing research to solve this multifaceted problem of concrete waste in the construction industry as well as waste from oyster shell farming. However, there is a lack of evidence on the possibility of producing a fully recycled composite consisting of recycled concrete and oyster shell without the need for new cement and natural aggregates. In this study, recycled concrete powder (RCP) and oyster shell were used to produce a green composite. Separate ground and combined ground (separate ground and co-ground) RCP and oyster shells are used to determine the effects of grinding approaches on the mechanical and chemical properties of the composite. The composite samples were molded via press molding by applying 30 MPa of pressure for 10 minutes. The results revealed that the composite prepared via the combined ground approach presented the highest flexural strength compared to the separate ground and unground samples. The FTIR and XRD characterization results revealed no chemical or phase alterations in the raw materials or the resulting composites before and after grinding. SEM analysis revealed that combined grinding reduced the particles’ size and improved the dispersion of the mixture, thereby increasing the strength.
文摘The restoration of European flat oyster (Ostrea edulis) population is critical for biodiversity and ecosystem services recovery. This study evaluates the potential of innovative clay substrates, developed and patented by Oyster Heaven, as cost-effective and degradable alternatives for large-scale oyster reef restoration. Field trials conducted in an oyster aquaculture site and hatchery experiments under controlled conditions assessed the larvae settlement efficiency, microbial biofilm composition, and environmental influences. Results showed significantly lower larvae recruitment on clay prototypes and spat collectors used as control, in wild conditions, with environmental factors such as temperature and hypoxia playing a key role. In the hatchery, larvae preferred oyster shells (17,174 ± 659 spats/m2) over clay prototypes (2917 ± 111 spats/m2) and spat collectors (1451 ± 160 spats/m2). Microbial analysis revealed a shift towards Cyanobacteria and Woeseia species, with Pseudoalteromonadaceae, a genus previously linked to both stimulating and inhibiting larvae settlement, detected only on clay surfaces. Despite challenges, the clay prototypes demonstrated potential as sustainable substrates for restoration, particularly in managed “spats-on-reefs” strategies. This research highlights the importance of environmental monitoring, microbial interactions, and substrate optimization in enhancing restoration success for O. edulis populations. Further investigations are recommended to refine clay designs and assess their broader applicability under variable environmental conditions.
基金supported by RIIM LPDP Grant and BRIN(B-3838/II.7.5/FR.06.00/11/2023)the Research Organization for Nanotechnology and Materials-National Research and Innovation Agency(BRIN)research grand 2025,the postdoctoral program of the National Research and Innovation Agency(BRIN),the Republic of Indonesia Decree Number 140/II/HK/2024.
文摘The increasing demand for renewable energy sources has driven the exploration of innovative materials for biofuel production.This study investigates bio-pellet characteristics derived from several oyster mushroom baglog wastes with varying concentrations of molasses as an adhesive.The process began with sun-drying the baglog waste for three days,followed by oven drying at 80○C for 24 h.Bio-pellets were produced by blending baglog waste with molasses at concentrations of 5%and 10%(w/v),then subsequently fed into a pellet mill.The bio-pellets were left to rest for one hour before analysis.The quality of bio-pellets was determined by evaluating moisture content,ash content,volatile matter,calorific value,combustion rate,density,and compressive strength following SNI 8675:2018 standards.Results indicate that adding molasses as a binder significantly affected the bio-pellet quality.The optimal molasses concentration for balanced performance was found at 5%,providing a lowermoisture content(6.8%),volatile matter(68.42%),and density(1.55 g⋅cm−3).In addition,the bio-pellet has a slightly higher calorific value(approximately 3614 cal⋅g−1),compressive strength(40.68 N⋅mm−2),and ash content(18.59%).All of the parameters for the bio-pellet containing 5%molasses satisfied the standard except for ash content and calorific value.
文摘Aceh in Indonesia is rich inmarine resources and abundant fishery products such as oyster.Traditionally,fishermen only harvest oysters and discard the shells,which can cause pollution and environmental contamination.Waste Oyster Shells(WOS)contain a high percentage of calcium carbonate(CaCO_(3))that experiences thermal decomposition at high temperature,following the reaction CaCO_(3)→CaO+CO_(2)(ΔT=825℃).At temperature>900℃,dead-burned lime is formed,which severely influences CaO reactivity.However,the optimum temperature for producing high CaO content is still uncertain.Therefore,this study aimed to determine the optimum calcination temperature to produce high CaO content,assess initial setting time of WOS paste,and identify the best compressive strength of paste.For the experiment,WOS was used as a partial cement replacement(with a size of 0.075 mm)in paste at a proportion of 5%and calcined at temperature of 700℃,800℃,900℃,and 1000℃.The specimens used were an ebonite ring(dimensions:70 mm bottom diameter,60 mm top diameter,and 40 mm height)and a cube(dimensions:5 cm×5 cm×5 cm).The experiment was conducted following the ASTM(American Society for Testing andMaterials)standards and optimumcompressive strength values were analyzed using ANOVA(Analysis of Variance)and Response Surface Methodology(RSM)through the Design Expert software.The results showed that WOS calcined at 1000℃ increased CaO content by approximately 57.40%.Furthermore,the initial setting time test of 5%WOS paste at 1000℃ showed a more uniform binding performance compared to conventional cement paste,with an initial setting time of 75 min and a penetration depth of 15 mm.In line with the analysis,optimum compressive strength of 71.028 MPa with a desirability value of 0.986 was achieved at 5%cement replacement and calcination temperature of 786.44℃.
基金The Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)under contract No.SML2021SP308the National Natural Science Foundation of China under contract Nos 42176173 and 42476268+1 种基金the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)under contract No.311020004Guangdong Geographical Science Data Center under contract No.2021B1212100003.
文摘Oyster farming provides substantial ecological and economic benefits but is often constrained by the challenges of selecting suitable sites in dynamic coastal environments.This study presents a tailored oyster suitability index(OSI)for the Zhujiang(Pearl)River Estuary(PRE),developed using Landsat satellite imagery and in situ observations collected from 2013 to 2023.Key environmental parameters,including sea surface temperature(SST),salinity,turbidity,and chlorophyll-a(Chl-a)concentration,were integrated for OSI retrieval.Optimal algorithms for each parameter were identified through evaluation using field measurements,yielding high accuracy,as evidenced by strong determination coefficients(R^(2))and low root mean square error(RMSE):R^(2)=0.98,RMSE=0.74℃for SST;R^(2)=0.94,RMSE=0.50 for salinity;R^(2)=0.95,RMSE=1.21 mg/m^(3)for Chl-a;R^(2)=0.91,RMSE=1.48 NTU for turbidity.The OSI revealed pronounced seasonal and spatial variability,with the highest suitability observed during winter and the lowest during summer.Validation results demonstrated strong alignment between OSI predictions and existing oyster farming zones.These findings underscore the value of remote sensing for scalable,near-real-time aquaculture site assessments.The OSI framework provides a robust decision-support tool for optimizing oyster cultivation,promoting sustainable aquaculture development in dynamic estuarine systems such as the PRE and beyond.
基金supported by the Japan Society for the Promotion of Science(No.19H05611).
文摘This study explores the relationship between cardiac activity and biochemical indicators in Pacific oysters(Crassostrea gigas)during cold storage to develop a nondestructive vitality assessment method.Oysters were stored at−1℃ for 14 d,with cardiac patterns tracked via image analysis,and biochemical markers(pH,adenosine triphosphate(ATP)-related compounds,and adenylate energy charge(AEC))were assessed.Five cardiac patterns were identified,with regular alternating contractions common early but decreasing over time,aligning with declines in AEC(44.11%-35.52%)and pH(6.98-6.55).The intervals between ventricular and atrial contractions rose from 4.2 to 5.6 s,strongly correlating with biochemical signs of vitality loss.Image analysis revealed characteristic waveforms for each cardiac pattern,despite amplitude variations caused by optical artifacts.These findings indicate that cardiac pattern analysis via image processing could be an effective nondestructive indicator of oyster vitality,offering a novel approach to quality control in shellfish storage and distribution.
文摘Biofouling is a particular problem in the pearl oyster culture. It may reduce the growth and survival rates of the cultured species. Foulers are often themselves filter feeders, and are therefore potential competitors for food resource with the cultured species. Fouling organisms may also reduce the oxygen supply. A study was conducted to measure the impact of foulers on feeding oxygen consumption, and waste excretion. POM, ammonia, phosphate and oxygen concentration were measured in various treatments （cultured species, foulers）. This study showed that fouling organisms had significant effect on food uptake, oxygen consumption and waste excretion. The clearance rate, ammonia and phosphate release rate, oxygen consumption rate of the fouled pearl oyster were greater than those of the clean pearl oysters. Other foulers that settled on cages or buoys also contributed much to phytoplankton depletion, oxygen consumption and concentration increase of ammonia and phosphate in water. Therefore, this study showed us that foulers were important competitors in the pearl oyster cultivation of Daya Bay in November 2005.
基金Supported by the National Key R&D Program of China(Nos.2022 YFD 2401301,2022 FY 100304)the National Natural Science Foundation of China(Nos.42076092,41776179)+1 种基金the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDB 42000000)the Earmarked Fund for Modern Agro-industry Technology Research System(No.CARS-47)。
文摘Oyster cultivation is a pivotal economic industry in Hainan Island,South China,where a high oyster species diversity is shown.However,the specific distribution and biodiversity of oyster resources in the island have remained unclear.To elucidate the diversity of oyster species and their distribution there,307 oyster samples were collected from 29 sites in the intertidal zone around the island,and were identified using both morphological and molecular approaches.A minimum of 12 oyster species were identified in taxonomy,including Crassostrea species(C.gigas angulata,C.sikamea,C.iredalei,C.dianbaiensis,C.talonata,C.ariakensis,and C.hongkongensis),and Saccostrea species(S.malabonensis,S.mordax,S.echinata,S.circumsuta,and S.mordoides).The results revealed a remarkable diversity of oyster species along the coast of the island.Particularly noteworthy are that S.malabonensis and S.mordax constituted 36%and 22%of the collected specimens,respectively.This study provided a comprehensive overview on current state of oyster biodiversity in Hainan,serving as a valuable reference for conservation and research on species distribution and resource dynamics in oyster populations.
文摘Concentrations of 8 heavy metals: cadmium (Cd), copper (Cu), zinc (Zn), lead (Pb), nickel (Ni), chromium (Cr), antimony (Sb) and tin (Sn) were examined in 3 species of bivalves (Perna viridis, Crassostrea rivularis and Ruditapes philippinarum) collected from 25 sites along the Pearl River Delta coastal waters in the South China Sea from July to August 1996. In general, Cd, Cu, Zn and Sn concentrations in the three bivalve species collected from the Estuarine Zone were significantly higher than those collected from the Western and Eastern Zones of the Pearl River Delta, which are related to the existence of various anthropogenic activities in the catchment of the Pearl River Delta. The Western Estuarine Zone is mainly impacted by Cr, Ni and Cu contamination. In Victoria Harbor, heavy metal contamination is mainly due to Cu and Pb. Cd, Cu and Zn concentrations in oysters were significantly higher than those in mussels and clams. This could be explained by the fact that oysters live mainly in the Estuarine Zone of the Pearl River Delta which receives most of the polluting discharges from the catchment of the Delta. During turbid condition, heavy metals(soluble or adsorbed on suspended particulates) discharged from the Delta are filtered from the water column and subsequently accumulated into the soft body tissues of oysters. Heavy metal concentrations in the three bivalve species were compared with the maximum permissible levels of heavy metals in seafood regulated by the Public Health and Municipal Services Ordinance, Laws of Hong Kong, and it was revealed that Cd and Cr concentrations in the three bivalve species exceeded the upper limits. At certain hotspots in the Delta, the maximum acceptable daily load for Cd was also exceeded.
基金supported by the Foundation of Tianjin University of Science and Technology(No.20120106)The International Science&Technology Cooperation Program of China(No.2013DFA31160)the Foundation of Tianjin Educational Committee(No.20090604)
文摘The incidence of diabetes has increased considerably, and become the third serious chronic disease following cancer and cardiovascular diseases. Though acarbose, metformin, and 1-deoxynojirimycin have good efficacy for clinical application as hypoglycemic drugs, their expensive costs and some degree of side effects have limited their clinical application. Recently, increasing attention has concentrated on the polysaccharides from natural plant and animal sources for diabetes. In order to illustrate the pharmaceutical activity of polysaccharides as natural hypoglycemic agents, polysaccharides isolated from Astragalus, oyster mushroom, and Yacon were evaluated for their inhibitory effects on α-glucosidase. Polysaccharides were extracted and purified from Astragalus, Oyster mushroom, and Yacon with hot water at 90 °C for 3 h, respectively. The total sugar content of the polysaccharide was determined by the phenol-sulfuric acid method. The α-glucosidase inhibitory activity was measured by the glucose oxidase method. The results exhibited that the inhibitory effects on α-glucosidase were in decreasing order, Astragalus > oyster mushroom > Yacon. The α-glucosidase inhibition percentage of Astragalus polysaccharide and oyster mushroom polysaccharide were over 40% at the polysaccharide concentration of 0.4 mg·mL-1. The IC50 of Astragalus polysaccharide and oyster mushroom polysaccharide were 0.28 and 0.424 mg·mL-1, respectively. The information obtained from this work is beneficial for the use polysaccharides as a dietary supplement for health foods and therapeutics for diabetes.
基金supported by the National Natural Science Foundation of China(No.51278318)the Key Scientific and Technological Projects of Sichuan Provincial Environmental Protection Bureau(No.2011HB001,2012HB002)+2 种基金the Science-Technology Support Plan Program from the Science and Technology Department of Sichuan Province(No.2013SZ0103)the State Key Laboratory of Hydraulics and Mountain River Engineering in China(No.1111)the Youth Foundation of the Sichuan Education Bureau(No.10ZB046)
文摘Estuaries have been described as one of the most difficult environments on Earth. It is difficult to know how to treat the combined wastewater in tidal rivers at the estuary, where the situation is very different from ordinary fresh water rivers. Waste oyster shell was used as the active filler in this study in a bio-contact oxidation tank to treat the combined wastewater at the Fengtang Tidal River. With a middle-experimental scale of 360 ma/day, the average removal efficiency of COD, BOD, NH3-N, TP and TSS was 80.05%, 85.02%, 86.59%, 50.58% and 85.32%, respectively, in this bio-contact oxidation process. The living microbes in the biofilms on the waste oyster shell in this bio-contact oxidation tank, which were mainly composed of zoogloea, protozoa and micro-metazoa species, revealed that waste oyster shell as the filler was suitable material for combined wastewater degradation. This treatment method using waste oyster shell as active filler was then applied in a mangrove demonstration area for water quality improvement near the experiment area, with a treatment volume of 5 × 10^3 m^3/day. Another project was also successfully applied in a constructed wetland, with a wastewater treatment volume of 1 ×10^3 m^3/day. This technology is therefore feasible and can easily be applied on a larger scale,
基金supported by the National Natural Science Foundation of China (No.31260376)the Natural Science Foundation of Hainan Province (No.317038)+1 种基金Primary Research & Development Plan of Hainan Province (No.ZDYF2017104)the Scientific Research Foundation of Hainan University (No.kyqd1662)
文摘In this study, Pearl oyster mantle type V collagen(POMC) and tilapia scale type I collagen(TSC) were extracted and hydrolyzed by various proteases in order to obtain peptides. The antioxidant activity of the peptides was investigated by DPPH, hydroxyl radical scavenging experiments and a dynamic digestion model in vitro. The results show that there are significant differences in amino acid composition between POMC and TSC. The collagen peptides obtained from pearl oyster mantle(POMCP) by treating with alkaline protease exhibited higher antioxidant activity than that from tilapia scale(TSCP) treated with papaya protease, and both of them showed greater DPPH and hydroxyl radical scavenging activity than other peptides. After being separated via Sephadex G-25 chromatography, the M1 fraction isolated from POMCP, and the S1 fraction from TSCP with which both had higher molecular weights showed the strongest antioxidant activity than other fractions, and the M1 fraction exhibited stronger antioxidant activity than the S1 fraction in scavenging free-radicals and protecting cells from the oxidation damage. Furthermore, after treating the dynamic digestion system model in vitro, the DPPH and hydroxyl radical scavenging activity of the M1 fraction increased slightly. These results suggest that POMCP exhibits stronger antioxidant activity than TSCP, which means that PMOP may be a good candidate to be a potential natural antioxidant in the food-processing industry.
基金financially supported by the National Natural Science Foundation of China (No. 31860442)the Natural Science Foundation of Guangxi Province, China (No. 2016GXNSFAA380067)
文摘Oyster peptide zinc nanoparticles (OPZNPs)(28 108 nm) were prepared in the presence of 0.5% 0.9% zinc sulfate at pH 6.0 11.0. The obtained nanoparticles exhibited uniform size distribution and spherical shapes. Nanoparticle characteristics, such as size, surface charge, and hydrophobicity, could be adjusted by controlling zinc sulfate concentration and environmental pH. In- creasing pH value or decreasing zinc sulfate concentration tended to reduce nanoparticle size and increase nanoparticle surface charge and hydrophobicity. OPZNPs presented good stability at near-neutral pH and could be stored for at least 20 days at 4℃. The results of the peptide conformation study and nanoparticle dissociation test proved that zinc ions and carboxyl groups are the key factors that affect OPZNP formation. The intermolecular combinations of carboxyl groups via zinc bridging facilitated the aggrega- tion of oyster peptides. Nanoparticle formation was accompanied by aggregate association and conformational changes. These changes included increments in β-sheets, especially intermolecular β-sheets, at the expense of α-helixes. Overall, this work provided a green alternative route for the synthesis of OPZNPs.
文摘The changes in the contents of adenosine 5’-triphosphate (ATP) and its related breakdown compounds were investigated in the adductor muscle, mantle, gill and body trunk of oyster (Crassostrea gigas) during frozen storage at -20℃ and -30℃ and compared with that of the fresh oyster. The investigation was performed using an HPLC system. Different extents of ATP decomposi- tion were found in various tissues frozen at the two temperatures. The K, K’ and A.E.C values were calculated as the chemical fresh- ness indices. Considering the results of sensory evaluation, the A.E.C. value in body trunk at -20℃ and -30℃ could be used as the best freshness index for frozen oyster.
基金Financial support by the National Natural Science Foundation of China(No.31301587)
文摘The effects of high pressure(HP) treatment on spoilage characteristic and shelf life extension of Pacific oysters(Crassostrea gigas) during refrigerated storage were studied.Results showed that HP treatment of 275 MPa for 3 min or 300 MPa for 2 min could achieve 100% full release of oyster adductor muscle,pressures higher than 350 MPa caused excessive release as the shells of oysters were broken,thus use of higher pressures should be cautious in oyster processing industry because of its adverse impact on the appearance of shells.HP treatment(300 MPa,2 min) was proper for the shucking of Pacific oyster(Crassostrea gigas) in China.This treatment caused no organoleptic disadvantage.Moreover,HP treatment resulted in obvious differences in biochemical spoilage indicators(p H,TVB-N and TBARS) changes and volatile compounds profile determined by electronic nose during storage.HP treatment(300 MPa,2 min) also led to a reduction of aerobic bacterial count(APC) by 1.27 log cycles.Furthermore,the APC values of oysters treated by HP were always lower than those of the control samples during storage.Based on the organoleptic,biochemical and microbiological indicators,shelf life of 6-8 d for control and 12 d for HP-treated oysters could be expected.HP treatment showed great potential in oyster processing and preservation.
基金Supported by the Special Fund of Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes(East China Sea Fisheries Research Institute)(No.2011M01)the National Natural Science Foundation of China(No.31170508)the National Basic Research Program of China(973 Program)(No.2010CB429005)
文摘Oyster reefs have an equivalent, complex 3-dimensional structure to vegetated habitats and may provide similar functions in estuarine environments. Nevertheless, few studies have compared oyster reefs with adjacent natural shallow-water habitats. Here the resident benthic macroinvertebrate communities in an artificial oyster （Crassostrea ariakensis） reef and in adjacent natural estuarine shallow-water habitats （salt marsh, intertidal mudflat, and subtidal soft bottom） in the Changjiang （Yangtze） River estuary were described. The mean total densities and biomass, Margalef＇s species richness, Pielou＇s evenness and Shannon-Weaver biodiversity indices of the resident benthic macroinvertebrate communities differed significantly among the habitats. Significantly higher densities and biomass of benthic macroinvertebrates occurred in the oyster reef compared with the other three habitats. Ordination plots showed a clear separation in benthic macroinvertebrate communities among the four habitat types. The results demonstrated that the artificial oyster reef supported distinct and unique benthic communities, playing an important role in the complex estuarine habitat by supplying prey resources and contributing to biodiversity. In addition, the results suggested that the oyster reef had been restored successfully.
基金Sponsored by the Fujian University New Century Excellent Talent Support Plan (No. XSJRC2007-17)
文摘Based on the analysis of adsorptive features of oyster shells,the researches on the treatment of phosphorous wastewater with oyster shells and the effect of temperature on phosphorus removal were carried out.XRD was used to characterize the crystalline phases,and the main component of oyster shells was shown to be CaCO3.When the pretreatment temperature reached 800 ℃,some CaCO3 decomposed into CaO.As the temperature was further raised,CaO increased gradually.Via SEM testing,the oyster shell was a kind of natural porous materials.The pore wall partially collapsed from 550 to 900 ℃.No obvious porous structure was found at 900 ℃.However,without preheating,the oyster shell phosphorous removal material can not adsorb the phosphorus.Pretreatment made calcium activate,thus greatly increasing the absorption of phosphorus.
基金Supported by the Natural Science Foundation of Fujian Province, China(NoC0510029)the Specialized Foundation for Creative Team of Young and Middle-aged Teachers of Jimei University, China(No2006A003)
文摘A light purple organic active substance capable of scavenging hydroxyl radical（OH） with a high efficiency was extracted from Oyster shell at an extraction rate of 2.49%. It was found for the first time that this active substance may scavenge OH with the efficiency far higher than that of vitamin C. This active substance may scavenge also superoxide radical（O2） although the scavenging efficiency is far lower than that of vitamin C. Infrared spectrometry and routine chemical analysis primarily reveal that this active substance belongs to glycoprotein.
基金supported by the National Natural Science Foundation of China(51409066,51761135013)the High Technology Ship Scientific Research Project from the Ministry of Industry and Information Technology of the People’s Republic of China–Floating Security Platform Project(the second stage,201622)the Fundamental Research Fund for the Central University (HEUCFJ180104,HEUCFP1809)
文摘A two-dimensional numerical Computational Fluid Dynamics(CFD)model is established on the basis of viscous CFD theory to investigate the motion response and power absorption performance of a bottom-hinged flap-type wave energy converter(WEC)under regular wave conditions.The convergence study of mesh size and time step is performed to ensure that wave height and motion response are sufficiently accurate.Wave height results reveal that the attenuation of wave height along the wave tank is less than 5%only if the suitable mesh size and time step are selected.The model proposed in this work is verified against published experimental and numerical models.The effects of mechanical damping,wave height,wave frequency,and water depth on the motion response,power generation,and energy conversion efficiency of the flap-type WEC are investigated.The selection of the appropriate mechanical damping of the WEC is crucial for the optimal extraction of wave power.The optimal mechanical damping can be readily predicted by using potential flow theory.It can then be verified by applying CFD numerical results.In addition,the motion response and the energy conversion efficiency of the WEC decrease as the incident wave height increases because the strengthened nonlinear effect of waves intensifies energy loss.Moreover,the energy conversion efficiency of theWEC decreases with increasing water depth and remains constant as the water depth reaches a critical value.Therefore,the selection of the optimal parameters during the design process is necessary to ensure that the WEC exhibits the maximum energy conversion efficiency.
基金supported by the grants from the National Natural Science Foundation of China (Nos. 3177 2843, 31741122)the Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province (No. 2017LZGC009)the Fundamental Research Funds for the Central Universities (No. 201762014)
文摘The Pacific oyster,Crassostrea gigas,naturally distributing along the coast of northwest Pacific,is one of the most important bivalve species due to its high economic value and fecundity.In China,we have initiated a selective breeding program on both shell color and growth rate of C.gigas since 2010.A black shell line was obtained through four-generation family selection.In this study,mass selection for growth improvement was conducted in the sixth generation and seventh generation of black shell lines.To assess the progress of potential genetic improvement,the progeny of two generations of black shell lines were selected to evaluate their shell heights via a 450-day farming experiment.As the results,after growing for 450 days,the sixth generation and seventh generation of selected lines were 9.03% and 11.42% larger than the control lines,respectively.During the grow-out stage,the genetic gain of two generations was 8.82%±0.18% and 11.54%±0.43%,respectively;and the corresponding realized heritability was 0.45±0.04 and 0.41±0.04,respectively.These results indicated that the mass selection for shell height achieved steady progress in the two generations of C.gigas.
