“What's in a name?That which we call a rose by any other name would smell as sweet”-William Shakespeare Polycystic ovary syndrome(PCOS)is one of the commonest endocrinopathy in the reproductive age group,as depi...“What's in a name?That which we call a rose by any other name would smell as sweet”-William Shakespeare Polycystic ovary syndrome(PCOS)is one of the commonest endocrinopathy in the reproductive age group,as depicted in a study among the North Indian population[1].The nomenclatures,the diagnostic criteria,the pathophysiology,and the modalities of treatment are full of controversies.Other names for this syndrome include Stein-Leventhal syndrome,polycystic ovary disease(PCOD),polycystic ovarian disease,functional ovarian hyperandrogenism,ovarian hyperthecosis and sclerocystic ovary syndrome,etc.展开更多
Recent studies have potentiated the essential role of androgens in normal follicu-logenesis and,therefore,female fertility.Contrastingly,excess androgen levels,i.e.,hyperandrogenism(HA),a hallmark characteristic of po...Recent studies have potentiated the essential role of androgens in normal follicu-logenesis and,therefore,female fertility.Contrastingly,excess androgen levels,i.e.,hyperandrogenism(HA),a hallmark characteristic of polycystic ovary syndrome,overrides the delicate balance of folliculogenesis,leading to follicular arrest and ovulatory issues.Insulin resistance(IR)has a profound effect on elevating androgen secretion and is considered one of the primary factors driving both ovarian androgen production and metabolic dysfunction in polycystic ovary syndrome.Together with IR,disruptions in key intraovarian and systemic factors,including activin,inhibin,follistatin,anti-Mullerian hormone,bone morpho-genetic proteins,growth differentiation factor-9 and Kit ligand,as well as dysreg-ulation in both the insulin and the transforming growth factor-βsuperfamily signaling pathway,contribute to follicular arrest,elevated androgen levels and metabolic dysfunction,exacerbating HA.Additionally,suppression of sex hormone-binding globulin,disrupted adipose-neuroendocrine signaling and altered microRNA expression heighten HA,with IR serving as the fundamental contributor.Emerging evidence implicates impaired atresia together with non-apoptotic cell death,such as ferroptosis and pyroptosis,which have also been associated with ovarian dysfunction.A comprehensive understanding of the most significant factors,particularly IR,which amplifies androgen production through hyperinsulinemia-mediated stimulation of theca cells,is essential for identifying targeted therapeutic strategies.展开更多
Objective:Polycystic ovary syndrome(PCOS)is a common endocrine disorder that affects women’s health.This study aims to investigate gene and transcription factor(TF)expression differences between PCOS patients and hea...Objective:Polycystic ovary syndrome(PCOS)is a common endocrine disorder that affects women’s health.This study aims to investigate gene and transcription factor(TF)expression differences between PCOS patients and healthy individuals using bioinformatics approaches,and to verify the function of key transcription factors,with the goal of providing new insights into the pathogenesis of PCOS.Methods:Differentially expressed genes(DEGs)and differentially expressed transcription factors(DETFs)between PCOS patients and controls were identified from the RNA sequencing dataset GSE168404 using bioinformatics methods.Functional enrichment analysis was performed using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)databases.The expression and function of core transcription factors were further validated in ovarian tissues of PCOS model mice and control mice using Western blotting and reverse transcription quantitative polymerase chain reaction(RTqPCR).Results:A total of 332 DEGs were identified between PCOS patients and controls,including 259 upregulated and 73 downregulated genes in the PCOS group.19 DETFs were further screened,of which 16 were upregulated and 3 were downregulated in PCOS.The upregulated DETFs(including TFCP2L1,DACH1,ESR2,AFF3,SMAD9,ZNF331,HOPX,ATOH8,HIF3α,DPF3,HOXC4,HES1,ID1,JDP2,SOX4,and ID3)were primarily associated with lipid metabolism,development,and cell adhesion.Protein and mRNA expression analysis in PCOS model mice revealed significantly decreased levels of hypoxia-inducible factor(HIF)1αand HIF2α,and significantly increased expression of HIF3αcompared to control mice(all P<0.001).Conclusion:Significant differences in gene and TF expression exist between PCOS patients and healthy individuals.HIF-3αmay play a crucial role in PCOS and could serve as a novel biomarker for diagnosis and a potential therapeutic target.展开更多
Polycystic ovary syndrome(PCOS)is the most common endocrine disease afflicting women of childbearing age.It is characterized by irregular menstruation,clinical or biochemical hyperandrogenemia,and polycystic ovary mor...Polycystic ovary syndrome(PCOS)is the most common endocrine disease afflicting women of childbearing age.It is characterized by irregular menstruation,clinical or biochemical hyperandrogenemia,and polycystic ovary morphology.As a complex endocrine-metabolic syndrome(MS),PCOS shares several endocrine-metabolic features with the MS,with insulin resistance at the core of their pathogenic mechanisms.PCOS and MS are interrelated and thus have similarities in treatment.Currently,the common treatment modalities for both are lifestyle intervention,medication,and surgery.More studies have shown that lifestyle intervention and regulation of intestinal flora are more effective and sustainable.However,progress towards treatment and cure is hampered by unclear etiology and mechanisms.This review aimed to summarize the relationship between PCOS and MS,the pathogenesis of metabolic disorders,and the current nutritional therapeutic strategies,especially lifestyle modifications and modulation of intestinal flora.Lifestyle interventions combined with the regulation of gut flora can be a new perspective for treatment.This perspective has a positive significance in the early diagnosis,adoption of personalized treatment plans,and prevention of complications in PCOS and MS.The need to prevent the occurrence of MS in patients with PCOS should be emphasized.展开更多
Background:Bonducellin is one of the bioactive compounds present in Caesalpinia bonduc Roxb(L).It is a homoisoflavonoid recognized for its anti-cancer,anti-androgenic,and anti-estrogenic properties and could potential...Background:Bonducellin is one of the bioactive compounds present in Caesalpinia bonduc Roxb(L).It is a homoisoflavonoid recognized for its anti-cancer,anti-androgenic,and anti-estrogenic properties and could potentially treat polycystic ovary syndrome(PCOS).However,the underlying molecular mechanism remains unexplored.This study aims to elucidate the potential molecular mechanisms of bonducellin in treating PCOS and its associated symptoms through an integrated approach combining network pharmacology,molecular docking,molecular dynamics simulation,and in vivo validation.Methods:Bonducellin-associated and PCOS-related genes were intersected using VENN analysis to determine common gene targets.KEGG pathway analysis was conducted to investigate the biological pathways involving the co-targeted genes.The protein-protein interactions of the target genes were performed to identify the key proteins interacting with bonducellin.Molecular docking and 100 ns molecular simulations were carried out to evaluate the binding affinity and conformational stability of bonducellin with the target proteins.Additionally,the acute toxicity of bonducellin was assessed on zebrafish embryos and in vivo gene expression studies were performed to examine its regulatory effect on the top co-targeted gene.Results:The intersection of bonducellin-associated and PCOS-related genes identified 76 co-targeted genes.KEGG pathway analysis revealed their involvement in 15 critical pathways,including steroid hormone biosynthesis.Protein-protein interaction and pathway enrichment analysis highlighted key targets,including MMP9,AR,KDR,PRKACA,KIT,CYP19A1,HSD11B1,ESR1,STAT3,ESR2,PRKCA,ROCK1,BRAF,HSD17B2,PIK3R1,and RAF1,all of which exhibited strong binding to bonducellin.Molecular simulations confirmed the stability of bonducellin to the top proteins,MMP9 and AR,with high binding scores.Acute toxicity studies in zebrafish embryos determined the LC50 value of bonducellin as 0.8μg/mL at 48 hpf.Gene expression analysis revealed that bonducellin differentially regulates the MMP9 gene that is involved in modulating PCOS-related pathways.Conclusion:This study suggests potential gene pathways and protein interactions through which bonducellin could exert therapeutic effects on PCOS and its associated disorders.This provides valuable insights for future research into understanding and developing bonducellin-based treatments for PCOS.展开更多
Objective:To investigate the distribution characteristics of sensory,sympathetic,and motor neurons as-sociated with the"Shenshu(BL 23)"and"Guanyuan(CV 4)"acupoints and the ovary,and to elucidate th...Objective:To investigate the distribution characteristics of sensory,sympathetic,and motor neurons as-sociated with the"Shenshu(BL 23)"and"Guanyuan(CV 4)"acupoints and the ovary,and to elucidate the neural anatomical connections between these acupoints and the ovary.Methods:In this study,dual fluorescent neural tracing was utilized,with twelve female Sprague-Dawley rats being randomly allocated into two groups(n=6 per group)for the purpose of administering tracer injections at distinct anatomical sites:the"BL 23"-ovary group received injections of Alexa Fluor 488-conjugated cholera toxin subunit B(AF488-CTB)at the left"BL 23"and Fluoro-Gold(FG)in the left ovary;the"CV 4"-ovary group received AF488-CTB at“CV 4”and FG in the left ovary.Three days post-injection,the distribution of AF488-CTB and FG-labeled neural components was observed in the dorsal root ganglia(DRGs),sympathetic chain,and spinal cord tissues.Results:①Sensory neurons labeled with AF488-CTB associated with"BL 23"were primarily distributed in the thoracic(T)10 to lumbar(L)2 DRGs,concentrated in T12-T13;postganglionic sympathetic neurons were mainly located in the thoracolumbar sympathetic chain;motor neurons were predominantly found in the anterior horn of the T11-L1 spinal cord segments.②Sensory neurons labeled with AF488-CTB associated with"CV 4"were primarily located in T10-L3 DRGs,concentrated in T13-L2 DRGs;postgan-glionic sympathetic neurons were mainly distributed in the lumbar sympathetic chain;motor neurons were primarily found in the anterior horn of T11-L2 spinal cord segments.③FG-labeled sensory neu-rons associated with the ovary were mainly distributed in the T9-L3 DRGs,concentrated in the T12-L2 DRGs;postganglionic sympathetic neurons were primarily located in the lumbar sympathetic chain.④Double-labeled(AF488-CTB and FG)sensory neurons associated with the"BL 23"and ovary were mainly distributed in the T12-T13 DRGs,with double-labeled postganglionic sympathetic neurons primarily in the lumbar sympathetic chain;double-labeled sensory neurons associated with the"CV 4"and ovary were mainly distributed in the T13-L1 DRGs,with double-labeled postganglionic sympathetic neurons primar-ily in the lumbar sympathetic chain.No double-labeled motor neurons were observed for either acupoint with the ovary.Conclusion:"BL 23"and"CV 4"and the ovary in rats exhibit segmental connections in sensory and sympathetic innervation,which may serve as the neural anatomical pathway and basis for acupuncture at these acupoints to regulate ovarian function.展开更多
Polycystic ovary syndrome(PCOS)is a prevalent endocrine disorder that poses a significant threat to women’s health.As a classical traditional Chinese medicine(TCM)formulation,Guizhi-Fuling Wan(GFW)has a good applicat...Polycystic ovary syndrome(PCOS)is a prevalent endocrine disorder that poses a significant threat to women’s health.As a classical traditional Chinese medicine(TCM)formulation,Guizhi-Fuling Wan(GFW)has a good application prospect in the complementary treatment of PCOS.This study aimed to systematically summarize the traditional efficacy and pharmacological composition of the Chinese medicines contained in GFW and evaluate the clinical efficacy and safety of their active ingredients in the complementary treatment of PCOS.A growing number of studies have demonstrated that GFW is effective at complementally treating PCOS through various mechanisms,including inhibiting inflammatory responses,modulating the intraovarian extracellular matrix,regulating apoptosis and autophagy in granulosa cells,and correcting oxidative stress imbalances.In addition,the GFW has been shown to be effective in treating the complications of PCOS.However,there are several problems,and future work should focus on elucidating the efficacy,safe dosage,and safety of different dosage forms of GFW,increasing the toxicological value of traditional Chinese medicine,and confirming the safety of the combination of GFW with Western medicine for the treatment of PCOS.展开更多
OBJECTIVE:To evaluate the effectiveness and safety of the sequential therapy in treating infertility with polycystic ovary syndrome(PCOS)and luteal phase defects(LPD)by Yangxin Dianji decoction(养心奠基方,YXDJ-D)and N...OBJECTIVE:To evaluate the effectiveness and safety of the sequential therapy in treating infertility with polycystic ovary syndrome(PCOS)and luteal phase defects(LPD)by Yangxin Dianji decoction(养心奠基方,YXDJ-D)and Nuangong Tiaojing decoction(暖宫调经方,NGTJ-D).METHODS:This study was undertaken in the Jiangsu Province Hospital of Chinese Medicine.Altogether 90 eligible patients with PCOS and LPD were assigned to exposed group A(Chinese Medicine therapy,YXDJ-D and NGTJ-D),exposed group B(Chinese Medicine plus Western Medicine therapy),control group(Western Medicine therapy).The exposed group A adopted the sequential therapy that YXDJ-D is taken in the postmenstrual period(follicular phase)and NGTJ-D is taken in premenstrual period(luteal phase).Control group took letrozole,dydrogesterone and was given intramuscular injection of human menopausal gonadotropin,human chorionic gonadotropin.The exposed group B was treated with the above-mentioned therapy project of integrated Chinese Medicine and Western Medicine.This study lasted for 2 courses for 6 months.The primary outcomes were pregnancy rate and early abortion rate.The secondary outcomes were the Traditional Chinese Medicine(TCM)syndrome scores,estrogen(E2)and progesterone(P),endometrial volume(EV),vascularity index(VI),flow index(FI)and vascularization flow index(VFI).These outcomes will be assessed at baseline and post-intervention.RESULTS:The pregnancy rates of the exposed group A and B were higher than the control group(60.00%vs 60.00%vs 53.33%),while early abortion rates of exposed groups A and B were lower than the control group(33.33%vs 16.67%vs 43.75%,P>0.05).Total efficacy rates in exposed group A and B were better than the control group(93.30%vs 93.30%vs 53.30%,P<0.01).TCM symptom scores and endometrial receptivity indexes(EV,FI,VFI)were significantly lower in exposed groups compared to the control group(P<0.05).P increase in exposed group B was superior to the other two groups(P<0.01).No noticeable abnormalities in safety indicators in the three groups.CONCLUSION:The sequential therapy of YXDJ-D and NGTJ-D can effectively increase pregnancy rate,reduce the early abortion rate and alleviate the clinical symptoms of infertility in patients with PCOS and LPD by improving luteal function and promoting the endometrial receptivity.展开更多
The ecdysone-induced transcription factor E93 in model insects plays multiple roles in the insect metamorphosis processes,such as remodeling larval tissues and determining adult tissue formation.The knockdown of E93in...The ecdysone-induced transcription factor E93 in model insects plays multiple roles in the insect metamorphosis processes,such as remodeling larval tissues and determining adult tissue formation.The knockdown of E93in insects leads to incomplete metamorphosis,suggesting that E93 is a potential target for pest control.In this study,the HaE93 gene in the cotton bollworm Helicoverpa armigera,a polyphagous pest of various commercial crops worldwide,was identified and found to have high expression in the egg,prepupal,and pupal stages.The injection of ds HaE93 induced about 60%mortality in H.armigera at the larval-pupal stage.About 30%survived but showed delayed pupation and abnormal wings,and the females developed reduced ovaries.Therefore,about 90%of the HaE93 knockdown individuals failed to reproduce before they died.The results of qRT-PCR showed that the expression levels of ecdysone primary-response genes,chitin synthesis-related genes,and wing and ovary development-related genes were reduced in HaE93 knockdown H.armigera.These results indicated that HaE93plays a critical role in larva-pupa-adult metamorphosis and the development of the cuticle,wing,and ovary in female H.armigera by regulating the expression of the associated genes.Bioassays of ds HaE93 administered by either oral delivery or injection showed similar knockdown results,which suggested that HaE93 can be used as a target gene for the RNAi control of the pest H.armigera.展开更多
Polycystic ovary syndrome(PCOS),a prevalent endocrine and metabolic disorder in women of reproductive age,necessitates standardized quality control criteria for diagnosis and treatment to minimize underdiagnosis,misdi...Polycystic ovary syndrome(PCOS),a prevalent endocrine and metabolic disorder in women of reproductive age,necessitates standardized quality control criteria for diagnosis and treatment to minimize underdiagnosis,misdiagnosis,and enhance long-term outcomes.This article outlines key quality control strategies for PCOS based on the latest domestic and international guidelines,integrating clinical experience.Diagnostic standardization requires structured protocols for medical history collection,evaluation of clinical manifestations(eg,menstrual irregularities,hyperandrogenism),and auxiliary examinations(hormonal profiling,ultrasound)to prevent overdiagnosis or oversight.Unified quality control standards can improve the consistency of PCOS management,optimize reproductive and metabolic outcomes,and reduce future disease burden.By implementing these measures,healthcare systems can achieve higher diagnostic accuracy,promote evidence-based therapeutic interventions,and mitigate long-term health risks associated with PCOS.展开更多
Polycystic ovary syndrome(PCOS)is a lifelong disorder affecting reproductive,metabolic,and psychological health.A healthy lifestyle and effective weight management strategies should underpin the treatment of PCOS.This...Polycystic ovary syndrome(PCOS)is a lifelong disorder affecting reproductive,metabolic,and psychological health.A healthy lifestyle and effective weight management strategies should underpin the treatment of PCOS.This case report documents the successful management of a Chinese patient with PCOS who was overweight and presented with oligomenorrhea and persistent adiposity.The patient was diagnosed using the Rotterdam criteria.The intervention was a 2-month program involving caloric restriction(1,350 kcal/day whole-food diet),progressive exercise titration(150–180 min/week of aerobic and resistance training),mindfulness practice,and metformin initiation after confirmed insulin resistance(HOMA-IR 3.67)resulted in clinically significant body composition improvements:fat mass was reduced by 5.0 kg,visceral adipose area was reduced by 44.7 cm^(2),skeletal muscle was increased by 1.4 kg,and regular menstrual cycles(32 day interval)were restored following 5% weight loss-consistent with evidence linking this threshold to improved ovarian function.The combination of a structured lifestyle modification program with targeted pharmacotherapy offers a viable clinical approach for metabolic PCOS phenotypes,although further validation is required to ascertain long-term efficacy.展开更多
As an important branch of traditional medicine,Zhuang Medicine is renowned for its unique prescriptions and techniques in clinical treatment,with significant therapeutic effects and widespread popularity.This article ...As an important branch of traditional medicine,Zhuang Medicine is renowned for its unique prescriptions and techniques in clinical treatment,with significant therapeutic effects and widespread popularity.This article introduces Professor Fang Gang’s experience in treating obesity-type polycystic ovary syndrome(PCOS)with modified Zhuang Medicine Mao Xia Yin.Professor Fang Gang proposes that the etiology and pathogenesis of obesity-type polycystic ovary syndrome(PCOS)primarily stem from spleen deficiency,which impairs the transport of water and dampness.This dysfunction leads to the accumulation of dampness that transforms into phlegm.Over time,the stagnation of phlegm and dampness generates internal heat,resulting in the formation of“dampness toxin,”“phlegm toxin,”and“heat toxin.”These pathological toxins obstruct the circulation of Qi and blood,causing stasis and the emergence of“stasis toxin.”The buildup of these toxins ultimately blocks the three channels and two pathways,disrupting the harmony among the three Qi of heaven,earth,and man.This disharmony culminates in impaired uterine gland function.Therefore,the treatment mainly focuses on tonifying deficiency,detoxifying,and regulating Qi.展开更多
The objective of this study was to set up a rhesus monkey model of polycystic ovary syndrome（PCOS）,which is globally prevalent among reproductive-aged human women,and to understand the reproductive traits of PCOS fe...The objective of this study was to set up a rhesus monkey model of polycystic ovary syndrome（PCOS）,which is globally prevalent among reproductive-aged human women,and to understand the reproductive traits of PCOS female monkeys.Six adult female rhesus monkeys aged 6-10 a,were divided into a PCOS group and a control group.The PCOS group were given two cycles of subcutaneous injections of propionic acid testosterone（PAT）,3.5 mg/kg body weight,on day 1,day 3,and day 5 of the menstrual cycle,respectively,and then given muscle injections of human chorionic gonadotropin（HCG）,350 IU/kg body wtight,on day 7,day 9,and day 11,respectively.Results showed that high levels of serum LH and T [（5.35±0.17） IU/L and（7.58±0.14） ng/mL,respectively],and a high ratio value of LH/FSH（5.35/1.30=4.12） were observed in the PCOS group.No significant differences were found in serum FSH,E2,and P in the PCOS group compared with those of the control.Polycystic ovaries in the PCOS monkeys were recorded by live ultrasound.The blastocysts rates of the PCOS vs.the control were 23.53% vs.66.67%,and there was a significant difference between the two groups.This study shows that PAT coupled with HCG can induce PCOS in rhesus monkeys in the short term.The reproductive features of PCOS monkeys were similar to those of PCOS patients.展开更多
[Objective] The aim was to discuss the histological characteristics of ovary development of Varicorhinus gerlachi.[Method] The annual variation of ovary and oocyte development of Varicorhinus gerlachi were observed an...[Objective] The aim was to discuss the histological characteristics of ovary development of Varicorhinus gerlachi.[Method] The annual variation of ovary and oocyte development of Varicorhinus gerlachi were observed and studied by means of histological method.[Result] Ovary development of Varicorhinus gerlachi was divided into 6 stages,and oocyte development had 6 phases,namely oocytes were in original differentiation state in phase Ⅰ,and yolk nucleus could be found obviously in phase Ⅱ.Vacuoles appeared in oocyte in phase Ⅲ,while oocytes differentiated obviously in phase Ⅳ,with the amalgamation of some yolk granules.Eggs formed and oocytes degenerated in phase V and Ⅵ.The development of ovary belonged to non-synchronous and multiple spawning type.In addition,Varicorhinus gerlachi began to spawn in April,and peak period was from May to July.[Conclusion] The study could provide technical parameters for the artificial propagation of Varicorhinus gerlachi.展开更多
Objective:To identify the alteration of tyrosine phosphorylated protein expression in rats with polycystic ovary syndrome(PCOS).Methods:Sixteen female Sprague-Dawley rats were divided into the control and letrozole-in...Objective:To identify the alteration of tyrosine phosphorylated protein expression in rats with polycystic ovary syndrome(PCOS).Methods:Sixteen female Sprague-Dawley rats were divided into the control and letrozole-induced PCOS groups.The oestrus cycle of rats was performed by vaginal smear.Sex hormones and morphology of the ovary,oviduct,and uterus were observed.Expressions and intensity of androgen receptor and tyrosine phosphorylated proteins of reproductive organs were investigated by Western blot.Results:Various polycysts and increased androgen receptor expression were present in the ovary of the PCOS group.The levels of follicle-stimulating hormone and testosteone were significantly higher in the PCOS group while progesterone and estradiol levels were significantly decreased as compared with the control group(P<0.05).Only the size of uterus in the PCOS group was significantly smaller than the control group.However,the density of collagen fibers observed in PCOS uterus was greater than the control group.Moreover,tyrosine phosphorylated proteins were significantly overexpressed in ovary(52,42,and 28 kDa),oviduct(72,56,42,and 28 kDa),and uterus(53 and 42 kDa)of the PCOS group compared to the control group.Conclusions:Presence of tyrosine phosphorylated proteins in the ovary,oviduct and uterus suggests that overexpression of tyrosine phosphorylated proteins may be involved in potential mechanism of female infertility especially in PCOS.展开更多
Objective To evaluate the differences of the clinical manifestation and endocrine situation in patients with different ovarian morphology of polycystic ovary syndrome （PCOS）. Methods A total of 234 PCOS patients wer...Objective To evaluate the differences of the clinical manifestation and endocrine situation in patients with different ovarian morphology of polycystic ovary syndrome （PCOS）. Methods A total of 234 PCOS patients were enrolled according to the ovary morphology and divided into three groups： 112 patients with B-polycystic ovary mor- phology （both two ovaries were PCOM, B-PCOM）, 50 with U-PCOM （only one ovary was PCOM） and 72 with N-PCOM （none was PCOM）. There were 39 infertile women without PCOS as control group. Data were analyzed by using SPSS 15.0 software. ResuIts There was no statistical difference in body mass index （BM1） among the three groups of PCOS. The endometrial thickness increased in patients with B-PCOM and decreased with N-PCOM. The levels of testosterone, androstenedione and luteinizing hormone increased in PCOS groups, especially in N-PCOM patients. HOMA-IR increased, HOMA-fl, disposition index （DI） and △160/AG60 decreased in patients with N- PCOM compared with in B-PCOM and U-PCOM groups. Higher level of total choles- terol （TC） and lower level of high-density lipoprotein （HDL）-C existed in PCOS patients, especially in N-PCOM. There were positive correlations between oligo-anovulation, endometrial thickness, LH/FSH ratio, fasting insulin （FINS）, the area under curve of glucose（A UCcLu） and PCOM, while there was a negative correlation between HOMA- IR and PCOM. Conclusion There are relationships among hyperandrogenism, hyperinsulinemia, insulin resistance （IR） and ovary morphology in PCOS patients. PCOS patients with- out PCOM have more serious IR and hyperandrogenism.展开更多
[Objective] This study was to investigate the in vitro culture of unfertilized ovary of strawberry.[Methods] Employing single factor experiment,we investigated six key factors including genotype,extrinsic hormone,sucr...[Objective] This study was to investigate the in vitro culture of unfertilized ovary of strawberry.[Methods] Employing single factor experiment,we investigated six key factors including genotype,extrinsic hormone,sucrose concentration,low temperature pretreatment,growth environment and development status,and illumination condition on induction of gynogenesis in vitro of unfertilized ovary,on the induction of gynogenesis in vitro of unfertilized ovary.[Results] The optimal conditions for in vitro culture of unfertilized ovary of strawberry were as follows：the primary flower buds cultured on bare land as explants,selection of appropriate genotype,2,4-D as external hormone,sucrose at the concentration of 6%,low temperature pretreatment for 48 hours and dark culture under alternated temperature.[Conclusion] The research provided reference for ploidy breeding in strawberry.展开更多
Objective This review aimed to systematically evaluate the evidence on the effects of acupoint catgut embedding （ACE） therapy for patients with polycystic ovary syndrome （PCOS）. Methods Five databases （CBM, CNKI,...Objective This review aimed to systematically evaluate the evidence on the effects of acupoint catgut embedding （ACE） therapy for patients with polycystic ovary syndrome （PCOS）. Methods Five databases （CBM, CNKI, Wanfang Database, VlP Database, and PubMed） were searched to identify relevant studies published before June 2027. The outcomes were resumption of menstruation and serum levels of testosterone （T）. The methodological quality of the included studies was judged using the Cochrane risk of bias tool. The overall level of evidence was judged by the Grading of Recommendations Assessment, Development, and Evaluation （GRADE） criteria. Results Twenty- five randomized controlled trials were included. ACE therapy significantly lowered serum T levels, and patients receiving ACE treatment reported resumption of menstruation. However, these results should be interpreted with caution due to a high risk of randomization and blinding bias, and likely publication bias. The level of evidence for resumption of menstruation and serum T levels was assessed as ＂low＂ and ＂low＂, respectively, using GRADE. Conclusion The current evidence on ACE therapy for PCOS is insufficient to draw firm conclusions due to the poor methodological quality. Future well- designed trials are needed to validate the therapeutic efficacy, safety, and mechanisms of ACE in patients with PCOS.展开更多
Objective To excavate and analyze the acupoint selection rules of acupuncture for PCOS. Methods A literature search was conducted in databases of Chinese Biomedicine Database（CBM）, Chinese National Knowledge Infrast...Objective To excavate and analyze the acupoint selection rules of acupuncture for PCOS. Methods A literature search was conducted in databases of Chinese Biomedicine Database（CBM）, Chinese National Knowledge Infrastructure Database（CNKI）, Wanfang Database, Chongqing VIP Chinese Science and Technology Periodical Database（VIP） and Pub Med. All clinical trials evaluating the effects of acupuncture in the treatment of polycystic ovary syndrome（PCOS） were included with or without methods of randomization and/or control. Four authors extracted information and two reviewers recorded information on titles, journals, interventions, main acupoints in selfestablished Excel sheet. Results The top ten acupoints of acupuncture therapy used most frequently were, by turns, Sānyīnjiāo（三阴交 SP 6）, Guānyuán（关元 CV 4）, Zhōngjí（中极 CV 3）, Zǐgōng（子宫 EX-CA 1）, Qìhǎi（气海 CV 6）, Zúsānlǐ（足三里 ST 36）, Shènshù（肾俞 BL 23）, Tiānshū（天枢 ST 25）, Guīlaí（归来 ST 29）, Zhōngwǎn（中脘 CV 12）. Conclusion Our study indicates that modern acupuncture treatment for PCOS is based on selection of specific acupoints according to traditional Chinese acupuncture theory. Points of spleen meridian, stomach meridian and conception vessel are chosen frequently which are most located on the abdomen and the lower limbs. Specific points used most are the front-mu points, the he-sea points, matching points and the back-shu points.展开更多
Objective To observe the effects of acupuncture on menstruation and endocrine in patients of normal body weight with polycystic ovary syndrome. Methods Twenty-seven patients were treated with acupuncture and moxibusti...Objective To observe the effects of acupuncture on menstruation and endocrine in patients of normal body weight with polycystic ovary syndrome. Methods Twenty-seven patients were treated with acupuncture and moxibustion for six months. Guanyuan （关元 4）, Zhongji （中极 CV 3）, and Zigong （子宫 EX-CA 1） on the abdomen and Sanyinjiao （三阴交SP 6）, Zusanli （足三里 ST 36）, Yinlingquan（阴陵泉 sP 9）, Fenglong （丰隆 ST 40）, xiaxi （侠溪GB 43）, Taichong （太冲 LR 3） were punctured. CV 3 on the abdomen and EX-CA 1 on both sides were manipulated by moxisbustion. The menstrual frequency, B ultrasound examination of uterus and adnexa uteri, luteinizing hormone （LH）, follicle stimulating hormone （FSH）, testosterone （T）, prolactin （PRL）, progesterone （PROG） and estradiol （E2） were recorded. Results Menstrual cycles were restored in twenty-six patients after six- month treatment. The total frequencies of menstruation were 105, of which 79 with normal ovulation accounting for 75.23%. The testosterone level of （109.65 ± 43.38） ng/dL before treatment were reduced to （53.7 ± 19.24） ng/dL after treatment, LH/FSH ratio of 4.15 ± 1.38 before treatment were reduced to 1.75 ± 1.09 after treatment. The differences were statistically significant （both P〈O.O5）. Conclusion Acupuncture can promote normal ovulation and menstrual cycles in patients with PCOS by regulating endocrine level.展开更多
文摘“What's in a name?That which we call a rose by any other name would smell as sweet”-William Shakespeare Polycystic ovary syndrome(PCOS)is one of the commonest endocrinopathy in the reproductive age group,as depicted in a study among the North Indian population[1].The nomenclatures,the diagnostic criteria,the pathophysiology,and the modalities of treatment are full of controversies.Other names for this syndrome include Stein-Leventhal syndrome,polycystic ovary disease(PCOD),polycystic ovarian disease,functional ovarian hyperandrogenism,ovarian hyperthecosis and sclerocystic ovary syndrome,etc.
基金Supported by Incentive Funding for Rated Researchers from the National Research Foundation,Pretoria,No.145943Research Reward from the Research Office of the University of KwaZulu-Natal,Durban,South Africa.
文摘Recent studies have potentiated the essential role of androgens in normal follicu-logenesis and,therefore,female fertility.Contrastingly,excess androgen levels,i.e.,hyperandrogenism(HA),a hallmark characteristic of polycystic ovary syndrome,overrides the delicate balance of folliculogenesis,leading to follicular arrest and ovulatory issues.Insulin resistance(IR)has a profound effect on elevating androgen secretion and is considered one of the primary factors driving both ovarian androgen production and metabolic dysfunction in polycystic ovary syndrome.Together with IR,disruptions in key intraovarian and systemic factors,including activin,inhibin,follistatin,anti-Mullerian hormone,bone morpho-genetic proteins,growth differentiation factor-9 and Kit ligand,as well as dysreg-ulation in both the insulin and the transforming growth factor-βsuperfamily signaling pathway,contribute to follicular arrest,elevated androgen levels and metabolic dysfunction,exacerbating HA.Additionally,suppression of sex hormone-binding globulin,disrupted adipose-neuroendocrine signaling and altered microRNA expression heighten HA,with IR serving as the fundamental contributor.Emerging evidence implicates impaired atresia together with non-apoptotic cell death,such as ferroptosis and pyroptosis,which have also been associated with ovarian dysfunction.A comprehensive understanding of the most significant factors,particularly IR,which amplifies androgen production through hyperinsulinemia-mediated stimulation of theca cells,is essential for identifying targeted therapeutic strategies.
基金supported by the Natural Science Foundation of Hunan Province,China(2022JJ30886).
文摘Objective:Polycystic ovary syndrome(PCOS)is a common endocrine disorder that affects women’s health.This study aims to investigate gene and transcription factor(TF)expression differences between PCOS patients and healthy individuals using bioinformatics approaches,and to verify the function of key transcription factors,with the goal of providing new insights into the pathogenesis of PCOS.Methods:Differentially expressed genes(DEGs)and differentially expressed transcription factors(DETFs)between PCOS patients and controls were identified from the RNA sequencing dataset GSE168404 using bioinformatics methods.Functional enrichment analysis was performed using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)databases.The expression and function of core transcription factors were further validated in ovarian tissues of PCOS model mice and control mice using Western blotting and reverse transcription quantitative polymerase chain reaction(RTqPCR).Results:A total of 332 DEGs were identified between PCOS patients and controls,including 259 upregulated and 73 downregulated genes in the PCOS group.19 DETFs were further screened,of which 16 were upregulated and 3 were downregulated in PCOS.The upregulated DETFs(including TFCP2L1,DACH1,ESR2,AFF3,SMAD9,ZNF331,HOPX,ATOH8,HIF3α,DPF3,HOXC4,HES1,ID1,JDP2,SOX4,and ID3)were primarily associated with lipid metabolism,development,and cell adhesion.Protein and mRNA expression analysis in PCOS model mice revealed significantly decreased levels of hypoxia-inducible factor(HIF)1αand HIF2α,and significantly increased expression of HIF3αcompared to control mice(all P<0.001).Conclusion:Significant differences in gene and TF expression exist between PCOS patients and healthy individuals.HIF-3αmay play a crucial role in PCOS and could serve as a novel biomarker for diagnosis and a potential therapeutic target.
基金Special Funds for Guiding Local Scientific and Technological Development by the Central Government(GuikeZY22096025)National Natural Science Foundation of China(32272316)Beijing Innovation Team of Livestock Industry Technology System(BAIC05-2022).
文摘Polycystic ovary syndrome(PCOS)is the most common endocrine disease afflicting women of childbearing age.It is characterized by irregular menstruation,clinical or biochemical hyperandrogenemia,and polycystic ovary morphology.As a complex endocrine-metabolic syndrome(MS),PCOS shares several endocrine-metabolic features with the MS,with insulin resistance at the core of their pathogenic mechanisms.PCOS and MS are interrelated and thus have similarities in treatment.Currently,the common treatment modalities for both are lifestyle intervention,medication,and surgery.More studies have shown that lifestyle intervention and regulation of intestinal flora are more effective and sustainable.However,progress towards treatment and cure is hampered by unclear etiology and mechanisms.This review aimed to summarize the relationship between PCOS and MS,the pathogenesis of metabolic disorders,and the current nutritional therapeutic strategies,especially lifestyle modifications and modulation of intestinal flora.Lifestyle interventions combined with the regulation of gut flora can be a new perspective for treatment.This perspective has a positive significance in the early diagnosis,adoption of personalized treatment plans,and prevention of complications in PCOS and MS.The need to prevent the occurrence of MS in patients with PCOS should be emphasized.
文摘Background:Bonducellin is one of the bioactive compounds present in Caesalpinia bonduc Roxb(L).It is a homoisoflavonoid recognized for its anti-cancer,anti-androgenic,and anti-estrogenic properties and could potentially treat polycystic ovary syndrome(PCOS).However,the underlying molecular mechanism remains unexplored.This study aims to elucidate the potential molecular mechanisms of bonducellin in treating PCOS and its associated symptoms through an integrated approach combining network pharmacology,molecular docking,molecular dynamics simulation,and in vivo validation.Methods:Bonducellin-associated and PCOS-related genes were intersected using VENN analysis to determine common gene targets.KEGG pathway analysis was conducted to investigate the biological pathways involving the co-targeted genes.The protein-protein interactions of the target genes were performed to identify the key proteins interacting with bonducellin.Molecular docking and 100 ns molecular simulations were carried out to evaluate the binding affinity and conformational stability of bonducellin with the target proteins.Additionally,the acute toxicity of bonducellin was assessed on zebrafish embryos and in vivo gene expression studies were performed to examine its regulatory effect on the top co-targeted gene.Results:The intersection of bonducellin-associated and PCOS-related genes identified 76 co-targeted genes.KEGG pathway analysis revealed their involvement in 15 critical pathways,including steroid hormone biosynthesis.Protein-protein interaction and pathway enrichment analysis highlighted key targets,including MMP9,AR,KDR,PRKACA,KIT,CYP19A1,HSD11B1,ESR1,STAT3,ESR2,PRKCA,ROCK1,BRAF,HSD17B2,PIK3R1,and RAF1,all of which exhibited strong binding to bonducellin.Molecular simulations confirmed the stability of bonducellin to the top proteins,MMP9 and AR,with high binding scores.Acute toxicity studies in zebrafish embryos determined the LC50 value of bonducellin as 0.8μg/mL at 48 hpf.Gene expression analysis revealed that bonducellin differentially regulates the MMP9 gene that is involved in modulating PCOS-related pathways.Conclusion:This study suggests potential gene pathways and protein interactions through which bonducellin could exert therapeutic effects on PCOS and its associated disorders.This provides valuable insights for future research into understanding and developing bonducellin-based treatments for PCOS.
基金Supported by the National Natural Science Foundation of China:82305409National Key Research and Development Program of China:2022YFC3500504。
文摘Objective:To investigate the distribution characteristics of sensory,sympathetic,and motor neurons as-sociated with the"Shenshu(BL 23)"and"Guanyuan(CV 4)"acupoints and the ovary,and to elucidate the neural anatomical connections between these acupoints and the ovary.Methods:In this study,dual fluorescent neural tracing was utilized,with twelve female Sprague-Dawley rats being randomly allocated into two groups(n=6 per group)for the purpose of administering tracer injections at distinct anatomical sites:the"BL 23"-ovary group received injections of Alexa Fluor 488-conjugated cholera toxin subunit B(AF488-CTB)at the left"BL 23"and Fluoro-Gold(FG)in the left ovary;the"CV 4"-ovary group received AF488-CTB at“CV 4”and FG in the left ovary.Three days post-injection,the distribution of AF488-CTB and FG-labeled neural components was observed in the dorsal root ganglia(DRGs),sympathetic chain,and spinal cord tissues.Results:①Sensory neurons labeled with AF488-CTB associated with"BL 23"were primarily distributed in the thoracic(T)10 to lumbar(L)2 DRGs,concentrated in T12-T13;postganglionic sympathetic neurons were mainly located in the thoracolumbar sympathetic chain;motor neurons were predominantly found in the anterior horn of the T11-L1 spinal cord segments.②Sensory neurons labeled with AF488-CTB associated with"CV 4"were primarily located in T10-L3 DRGs,concentrated in T13-L2 DRGs;postgan-glionic sympathetic neurons were mainly distributed in the lumbar sympathetic chain;motor neurons were primarily found in the anterior horn of T11-L2 spinal cord segments.③FG-labeled sensory neu-rons associated with the ovary were mainly distributed in the T9-L3 DRGs,concentrated in the T12-L2 DRGs;postganglionic sympathetic neurons were primarily located in the lumbar sympathetic chain.④Double-labeled(AF488-CTB and FG)sensory neurons associated with the"BL 23"and ovary were mainly distributed in the T12-T13 DRGs,with double-labeled postganglionic sympathetic neurons primarily in the lumbar sympathetic chain;double-labeled sensory neurons associated with the"CV 4"and ovary were mainly distributed in the T13-L1 DRGs,with double-labeled postganglionic sympathetic neurons primar-ily in the lumbar sympathetic chain.No double-labeled motor neurons were observed for either acupoint with the ovary.Conclusion:"BL 23"and"CV 4"and the ovary in rats exhibit segmental connections in sensory and sympathetic innervation,which may serve as the neural anatomical pathway and basis for acupuncture at these acupoints to regulate ovarian function.
基金supported by funds from the Natural Science Foundation of Shandong Province(No.ZR2020MH346No.ZR2021MH003)the National Natural Science Foundation of China(No.81273667).
文摘Polycystic ovary syndrome(PCOS)is a prevalent endocrine disorder that poses a significant threat to women’s health.As a classical traditional Chinese medicine(TCM)formulation,Guizhi-Fuling Wan(GFW)has a good application prospect in the complementary treatment of PCOS.This study aimed to systematically summarize the traditional efficacy and pharmacological composition of the Chinese medicines contained in GFW and evaluate the clinical efficacy and safety of their active ingredients in the complementary treatment of PCOS.A growing number of studies have demonstrated that GFW is effective at complementally treating PCOS through various mechanisms,including inhibiting inflammatory responses,modulating the intraovarian extracellular matrix,regulating apoptosis and autophagy in granulosa cells,and correcting oxidative stress imbalances.In addition,the GFW has been shown to be effective in treating the complications of PCOS.However,there are several problems,and future work should focus on elucidating the efficacy,safe dosage,and safety of different dosage forms of GFW,increasing the toxicological value of traditional Chinese medicine,and confirming the safety of the combination of GFW with Western medicine for the treatment of PCOS.
基金Supported by National Natural Science Foundation of China:Elucidation of a New Mechanism of the Prescription for Reinforcing Kidney and Promoting Pregnancy (Bushen Zhuyun Decoction) in Treating Luteal Phase Defects Infertility based on Coenzyme Q10/Ubiquinol Mediated New Ferroptosis Pathway in Endometrium During Implantation (No. 82474567)Jiangsu Province Key Research and Development Plan (Society Development) Project:a Multicenter Real-world Study of Sequential Treatment of Luteal Phase Defects Infertility with “Improving Endometrial Receptivity” as the Effect Point (No. BE2021726)Project of Clinical Innovation Center of Traditional Chinese Medicine in Jiangsu Province:Clinical And Basic Research on the Treatment of Luteal Phase Defects Infertility with “Improving Endometrial Receptivity” as the Effect Point (No. k2021j18-1)
文摘OBJECTIVE:To evaluate the effectiveness and safety of the sequential therapy in treating infertility with polycystic ovary syndrome(PCOS)and luteal phase defects(LPD)by Yangxin Dianji decoction(养心奠基方,YXDJ-D)and Nuangong Tiaojing decoction(暖宫调经方,NGTJ-D).METHODS:This study was undertaken in the Jiangsu Province Hospital of Chinese Medicine.Altogether 90 eligible patients with PCOS and LPD were assigned to exposed group A(Chinese Medicine therapy,YXDJ-D and NGTJ-D),exposed group B(Chinese Medicine plus Western Medicine therapy),control group(Western Medicine therapy).The exposed group A adopted the sequential therapy that YXDJ-D is taken in the postmenstrual period(follicular phase)and NGTJ-D is taken in premenstrual period(luteal phase).Control group took letrozole,dydrogesterone and was given intramuscular injection of human menopausal gonadotropin,human chorionic gonadotropin.The exposed group B was treated with the above-mentioned therapy project of integrated Chinese Medicine and Western Medicine.This study lasted for 2 courses for 6 months.The primary outcomes were pregnancy rate and early abortion rate.The secondary outcomes were the Traditional Chinese Medicine(TCM)syndrome scores,estrogen(E2)and progesterone(P),endometrial volume(EV),vascularity index(VI),flow index(FI)and vascularization flow index(VFI).These outcomes will be assessed at baseline and post-intervention.RESULTS:The pregnancy rates of the exposed group A and B were higher than the control group(60.00%vs 60.00%vs 53.33%),while early abortion rates of exposed groups A and B were lower than the control group(33.33%vs 16.67%vs 43.75%,P>0.05).Total efficacy rates in exposed group A and B were better than the control group(93.30%vs 93.30%vs 53.30%,P<0.01).TCM symptom scores and endometrial receptivity indexes(EV,FI,VFI)were significantly lower in exposed groups compared to the control group(P<0.05).P increase in exposed group B was superior to the other two groups(P<0.01).No noticeable abnormalities in safety indicators in the three groups.CONCLUSION:The sequential therapy of YXDJ-D and NGTJ-D can effectively increase pregnancy rate,reduce the early abortion rate and alleviate the clinical symptoms of infertility in patients with PCOS and LPD by improving luteal function and promoting the endometrial receptivity.
基金supported by the National Natural Science Foundation of China(32001912 and 32370525)the Henan Provincial Natural Science Foundation,China(232300420012)。
文摘The ecdysone-induced transcription factor E93 in model insects plays multiple roles in the insect metamorphosis processes,such as remodeling larval tissues and determining adult tissue formation.The knockdown of E93in insects leads to incomplete metamorphosis,suggesting that E93 is a potential target for pest control.In this study,the HaE93 gene in the cotton bollworm Helicoverpa armigera,a polyphagous pest of various commercial crops worldwide,was identified and found to have high expression in the egg,prepupal,and pupal stages.The injection of ds HaE93 induced about 60%mortality in H.armigera at the larval-pupal stage.About 30%survived but showed delayed pupation and abnormal wings,and the females developed reduced ovaries.Therefore,about 90%of the HaE93 knockdown individuals failed to reproduce before they died.The results of qRT-PCR showed that the expression levels of ecdysone primary-response genes,chitin synthesis-related genes,and wing and ovary development-related genes were reduced in HaE93 knockdown H.armigera.These results indicated that HaE93plays a critical role in larva-pupa-adult metamorphosis and the development of the cuticle,wing,and ovary in female H.armigera by regulating the expression of the associated genes.Bioassays of ds HaE93 administered by either oral delivery or injection showed similar knockdown results,which suggested that HaE93 can be used as a target gene for the RNAi control of the pest H.armigera.
文摘Polycystic ovary syndrome(PCOS),a prevalent endocrine and metabolic disorder in women of reproductive age,necessitates standardized quality control criteria for diagnosis and treatment to minimize underdiagnosis,misdiagnosis,and enhance long-term outcomes.This article outlines key quality control strategies for PCOS based on the latest domestic and international guidelines,integrating clinical experience.Diagnostic standardization requires structured protocols for medical history collection,evaluation of clinical manifestations(eg,menstrual irregularities,hyperandrogenism),and auxiliary examinations(hormonal profiling,ultrasound)to prevent overdiagnosis or oversight.Unified quality control standards can improve the consistency of PCOS management,optimize reproductive and metabolic outcomes,and reduce future disease burden.By implementing these measures,healthcare systems can achieve higher diagnostic accuracy,promote evidence-based therapeutic interventions,and mitigate long-term health risks associated with PCOS.
文摘Polycystic ovary syndrome(PCOS)is a lifelong disorder affecting reproductive,metabolic,and psychological health.A healthy lifestyle and effective weight management strategies should underpin the treatment of PCOS.This case report documents the successful management of a Chinese patient with PCOS who was overweight and presented with oligomenorrhea and persistent adiposity.The patient was diagnosed using the Rotterdam criteria.The intervention was a 2-month program involving caloric restriction(1,350 kcal/day whole-food diet),progressive exercise titration(150–180 min/week of aerobic and resistance training),mindfulness practice,and metformin initiation after confirmed insulin resistance(HOMA-IR 3.67)resulted in clinically significant body composition improvements:fat mass was reduced by 5.0 kg,visceral adipose area was reduced by 44.7 cm^(2),skeletal muscle was increased by 1.4 kg,and regular menstrual cycles(32 day interval)were restored following 5% weight loss-consistent with evidence linking this threshold to improved ovarian function.The combination of a structured lifestyle modification program with targeted pharmacotherapy offers a viable clinical approach for metabolic PCOS phenotypes,although further validation is required to ascertain long-term efficacy.
基金National Administration of Traditional Chinese Medicine high-level key discipline construction project on Ethnic Minority Medicine(Zhuang Medicine)(Project No.:zyyzdxk-2023164)Guangxi Higher Education Key Laboratory for Research on Toxin-related Diseases in Zhuang Medicine(Guijiao Keyan[2022]No.10).
文摘As an important branch of traditional medicine,Zhuang Medicine is renowned for its unique prescriptions and techniques in clinical treatment,with significant therapeutic effects and widespread popularity.This article introduces Professor Fang Gang’s experience in treating obesity-type polycystic ovary syndrome(PCOS)with modified Zhuang Medicine Mao Xia Yin.Professor Fang Gang proposes that the etiology and pathogenesis of obesity-type polycystic ovary syndrome(PCOS)primarily stem from spleen deficiency,which impairs the transport of water and dampness.This dysfunction leads to the accumulation of dampness that transforms into phlegm.Over time,the stagnation of phlegm and dampness generates internal heat,resulting in the formation of“dampness toxin,”“phlegm toxin,”and“heat toxin.”These pathological toxins obstruct the circulation of Qi and blood,causing stasis and the emergence of“stasis toxin.”The buildup of these toxins ultimately blocks the three channels and two pathways,disrupting the harmony among the three Qi of heaven,earth,and man.This disharmony culminates in impaired uterine gland function.Therefore,the treatment mainly focuses on tonifying deficiency,detoxifying,and regulating Qi.
基金supported by Yunnan Key Laboratory of Animal Reproductive Biology(2010-03)
文摘The objective of this study was to set up a rhesus monkey model of polycystic ovary syndrome（PCOS）,which is globally prevalent among reproductive-aged human women,and to understand the reproductive traits of PCOS female monkeys.Six adult female rhesus monkeys aged 6-10 a,were divided into a PCOS group and a control group.The PCOS group were given two cycles of subcutaneous injections of propionic acid testosterone（PAT）,3.5 mg/kg body weight,on day 1,day 3,and day 5 of the menstrual cycle,respectively,and then given muscle injections of human chorionic gonadotropin（HCG）,350 IU/kg body wtight,on day 7,day 9,and day 11,respectively.Results showed that high levels of serum LH and T [（5.35±0.17） IU/L and（7.58±0.14） ng/mL,respectively],and a high ratio value of LH/FSH（5.35/1.30=4.12） were observed in the PCOS group.No significant differences were found in serum FSH,E2,and P in the PCOS group compared with those of the control.Polycystic ovaries in the PCOS monkeys were recorded by live ultrasound.The blastocysts rates of the PCOS vs.the control were 23.53% vs.66.67%,and there was a significant difference between the two groups.This study shows that PAT coupled with HCG can induce PCOS in rhesus monkeys in the short term.The reproductive features of PCOS monkeys were similar to those of PCOS patients.
文摘[Objective] The aim was to discuss the histological characteristics of ovary development of Varicorhinus gerlachi.[Method] The annual variation of ovary and oocyte development of Varicorhinus gerlachi were observed and studied by means of histological method.[Result] Ovary development of Varicorhinus gerlachi was divided into 6 stages,and oocyte development had 6 phases,namely oocytes were in original differentiation state in phase Ⅰ,and yolk nucleus could be found obviously in phase Ⅱ.Vacuoles appeared in oocyte in phase Ⅲ,while oocytes differentiated obviously in phase Ⅳ,with the amalgamation of some yolk granules.Eggs formed and oocytes degenerated in phase V and Ⅵ.The development of ovary belonged to non-synchronous and multiple spawning type.In addition,Varicorhinus gerlachi began to spawn in April,and peak period was from May to July.[Conclusion] The study could provide technical parameters for the artificial propagation of Varicorhinus gerlachi.
基金This study was supported by Invitation Research Grant,Faculty of Medicine,Khon Kaen University,Thailand(Grant No.IN62336).
文摘Objective:To identify the alteration of tyrosine phosphorylated protein expression in rats with polycystic ovary syndrome(PCOS).Methods:Sixteen female Sprague-Dawley rats were divided into the control and letrozole-induced PCOS groups.The oestrus cycle of rats was performed by vaginal smear.Sex hormones and morphology of the ovary,oviduct,and uterus were observed.Expressions and intensity of androgen receptor and tyrosine phosphorylated proteins of reproductive organs were investigated by Western blot.Results:Various polycysts and increased androgen receptor expression were present in the ovary of the PCOS group.The levels of follicle-stimulating hormone and testosteone were significantly higher in the PCOS group while progesterone and estradiol levels were significantly decreased as compared with the control group(P<0.05).Only the size of uterus in the PCOS group was significantly smaller than the control group.However,the density of collagen fibers observed in PCOS uterus was greater than the control group.Moreover,tyrosine phosphorylated proteins were significantly overexpressed in ovary(52,42,and 28 kDa),oviduct(72,56,42,and 28 kDa),and uterus(53 and 42 kDa)of the PCOS group compared to the control group.Conclusions:Presence of tyrosine phosphorylated proteins in the ovary,oviduct and uterus suggests that overexpression of tyrosine phosphorylated proteins may be involved in potential mechanism of female infertility especially in PCOS.
文摘Objective To evaluate the differences of the clinical manifestation and endocrine situation in patients with different ovarian morphology of polycystic ovary syndrome （PCOS）. Methods A total of 234 PCOS patients were enrolled according to the ovary morphology and divided into three groups： 112 patients with B-polycystic ovary mor- phology （both two ovaries were PCOM, B-PCOM）, 50 with U-PCOM （only one ovary was PCOM） and 72 with N-PCOM （none was PCOM）. There were 39 infertile women without PCOS as control group. Data were analyzed by using SPSS 15.0 software. ResuIts There was no statistical difference in body mass index （BM1） among the three groups of PCOS. The endometrial thickness increased in patients with B-PCOM and decreased with N-PCOM. The levels of testosterone, androstenedione and luteinizing hormone increased in PCOS groups, especially in N-PCOM patients. HOMA-IR increased, HOMA-fl, disposition index （DI） and △160/AG60 decreased in patients with N- PCOM compared with in B-PCOM and U-PCOM groups. Higher level of total choles- terol （TC） and lower level of high-density lipoprotein （HDL）-C existed in PCOS patients, especially in N-PCOM. There were positive correlations between oligo-anovulation, endometrial thickness, LH/FSH ratio, fasting insulin （FINS）, the area under curve of glucose（A UCcLu） and PCOM, while there was a negative correlation between HOMA- IR and PCOM. Conclusion There are relationships among hyperandrogenism, hyperinsulinemia, insulin resistance （IR） and ovary morphology in PCOS patients. PCOS patients with- out PCOM have more serious IR and hyperandrogenism.
基金Supported by Program from Beijing Municipal Science & Technology Commission (Z080005032508017)~~
文摘[Objective] This study was to investigate the in vitro culture of unfertilized ovary of strawberry.[Methods] Employing single factor experiment,we investigated six key factors including genotype,extrinsic hormone,sucrose concentration,low temperature pretreatment,growth environment and development status,and illumination condition on induction of gynogenesis in vitro of unfertilized ovary,on the induction of gynogenesis in vitro of unfertilized ovary.[Results] The optimal conditions for in vitro culture of unfertilized ovary of strawberry were as follows：the primary flower buds cultured on bare land as explants,selection of appropriate genotype,2,4-D as external hormone,sucrose at the concentration of 6%,low temperature pretreatment for 48 hours and dark culture under alternated temperature.[Conclusion] The research provided reference for ploidy breeding in strawberry.
文摘Objective This review aimed to systematically evaluate the evidence on the effects of acupoint catgut embedding （ACE） therapy for patients with polycystic ovary syndrome （PCOS）. Methods Five databases （CBM, CNKI, Wanfang Database, VlP Database, and PubMed） were searched to identify relevant studies published before June 2027. The outcomes were resumption of menstruation and serum levels of testosterone （T）. The methodological quality of the included studies was judged using the Cochrane risk of bias tool. The overall level of evidence was judged by the Grading of Recommendations Assessment, Development, and Evaluation （GRADE） criteria. Results Twenty- five randomized controlled trials were included. ACE therapy significantly lowered serum T levels, and patients receiving ACE treatment reported resumption of menstruation. However, these results should be interpreted with caution due to a high risk of randomization and blinding bias, and likely publication bias. The level of evidence for resumption of menstruation and serum T levels was assessed as ＂low＂ and ＂low＂, respectively, using GRADE. Conclusion The current evidence on ACE therapy for PCOS is insufficient to draw firm conclusions due to the poor methodological quality. Future well- designed trials are needed to validate the therapeutic efficacy, safety, and mechanisms of ACE in patients with PCOS.
文摘Objective To excavate and analyze the acupoint selection rules of acupuncture for PCOS. Methods A literature search was conducted in databases of Chinese Biomedicine Database（CBM）, Chinese National Knowledge Infrastructure Database（CNKI）, Wanfang Database, Chongqing VIP Chinese Science and Technology Periodical Database（VIP） and Pub Med. All clinical trials evaluating the effects of acupuncture in the treatment of polycystic ovary syndrome（PCOS） were included with or without methods of randomization and/or control. Four authors extracted information and two reviewers recorded information on titles, journals, interventions, main acupoints in selfestablished Excel sheet. Results The top ten acupoints of acupuncture therapy used most frequently were, by turns, Sānyīnjiāo（三阴交 SP 6）, Guānyuán（关元 CV 4）, Zhōngjí（中极 CV 3）, Zǐgōng（子宫 EX-CA 1）, Qìhǎi（气海 CV 6）, Zúsānlǐ（足三里 ST 36）, Shènshù（肾俞 BL 23）, Tiānshū（天枢 ST 25）, Guīlaí（归来 ST 29）, Zhōngwǎn（中脘 CV 12）. Conclusion Our study indicates that modern acupuncture treatment for PCOS is based on selection of specific acupoints according to traditional Chinese acupuncture theory. Points of spleen meridian, stomach meridian and conception vessel are chosen frequently which are most located on the abdomen and the lower limbs. Specific points used most are the front-mu points, the he-sea points, matching points and the back-shu points.
文摘Objective To observe the effects of acupuncture on menstruation and endocrine in patients of normal body weight with polycystic ovary syndrome. Methods Twenty-seven patients were treated with acupuncture and moxibustion for six months. Guanyuan （关元 4）, Zhongji （中极 CV 3）, and Zigong （子宫 EX-CA 1） on the abdomen and Sanyinjiao （三阴交SP 6）, Zusanli （足三里 ST 36）, Yinlingquan（阴陵泉 sP 9）, Fenglong （丰隆 ST 40）, xiaxi （侠溪GB 43）, Taichong （太冲 LR 3） were punctured. CV 3 on the abdomen and EX-CA 1 on both sides were manipulated by moxisbustion. The menstrual frequency, B ultrasound examination of uterus and adnexa uteri, luteinizing hormone （LH）, follicle stimulating hormone （FSH）, testosterone （T）, prolactin （PRL）, progesterone （PROG） and estradiol （E2） were recorded. Results Menstrual cycles were restored in twenty-six patients after six- month treatment. The total frequencies of menstruation were 105, of which 79 with normal ovulation accounting for 75.23%. The testosterone level of （109.65 ± 43.38） ng/dL before treatment were reduced to （53.7 ± 19.24） ng/dL after treatment, LH/FSH ratio of 4.15 ± 1.38 before treatment were reduced to 1.75 ± 1.09 after treatment. The differences were statistically significant （both P〈O.O5）. Conclusion Acupuncture can promote normal ovulation and menstrual cycles in patients with PCOS by regulating endocrine level.
