去泛素化酶含OTU结构域蛋白3(OTU domain-containing protein 3,OTUD3)是非常重要的去泛素化酶,在多种疾病模型中存在明显的表达差异。在不同细胞中,OTUD3参与调控凋亡、炎症反应、细胞周期、增殖和侵袭等生物学功能。OTUD3通过调节多...去泛素化酶含OTU结构域蛋白3(OTU domain-containing protein 3,OTUD3)是非常重要的去泛素化酶,在多种疾病模型中存在明显的表达差异。在不同细胞中,OTUD3参与调控凋亡、炎症反应、细胞周期、增殖和侵袭等生物学功能。OTUD3通过调节多种关键底物蛋白的去泛素化,在先天性抗病毒免疫、神经发育、神经系统退行性疾病、肿瘤等生理和病理过程中发挥重要作用。OTUD3在乳腺癌、食管癌、结肠癌、甲状腺乳头状癌中发挥抑癌作用,但在肝癌和肺癌中发挥促癌作用。作为恶性肿瘤的生物标志物,OTUD3在预测、诊断和判断预后方面起重要作用。OTUD3在大部分疾病中的相关分子机制研究仍不够深入,研究OTUD3的调控机制对于深入了解其在免疫和疾病中的作用至关重要,以OTUD3为靶标的药物研发将是今后的研究重点。展开更多
Zebrafish（Danio rerio） Z-OTU,containing OTU and TUDOR domains,was predicted to be a member of OTU-related protease,a family of the deubiquitylating enzymes（DUBs）.A previous report from our laboratory clearly descr...Zebrafish（Danio rerio） Z-OTU,containing OTU and TUDOR domains,was predicted to be a member of OTU-related protease,a family of the deubiquitylating enzymes（DUBs）.A previous report from our laboratory clearly describes the expression patterns of z-otu mRNA.Here,we characterized the Z-OTU protein during zebrafish oogenesis and early embryogenesis.After prokaryotic expression,the recombinant protein of the OTU domain and GST was purified and injected into rabbits to obtain the polyclonal antibody-anti-Z-OTU,which was used for immunohistochemistry in zebrafish ovaries and embryos.Interestingly,obvious differences existed between the expression patterns of z-otu mRNA and its protein during oogenesis and early embryogenesis.In stage I oocytes,z-otu mRNA was detected in cytoplasm while its protein existed in the germinal vesicle.In addition,its protein was distributed during entire oogenesis,while mRNA was not detected in oocytes at stage IV or mature oocytes.The z-otu mRNA disappeared after midblastula transition（MBT） and its protein gradually decreased after this stage.We inferred that Z-OTU protein,like other OTU-related protease with DUB activity,was required for germinal vesicle breakdown of oocytes during meiosis,germinal vesicle migration,and embryo cleavage maintenance.展开更多
瑞士Avancon股份有限公司生产的万向传输单元(Omni-directional Transfer Unit,OTU),可应用在内部物流系统的输送装置上。OTU可用于进料装置、开关、转向器、交叉点、输送器、分拣机等,实现任意方向的输送;或组合成任意长度和宽度的OTU...瑞士Avancon股份有限公司生产的万向传输单元(Omni-directional Transfer Unit,OTU),可应用在内部物流系统的输送装置上。OTU可用于进料装置、开关、转向器、交叉点、输送器、分拣机等,实现任意方向的输送;或组合成任意长度和宽度的OTU换向台,并以现场总线为接口,建立起一套完整的控制系统。每个OTU都由尺寸为75×75×133毫米的高科技聚合物材料构成,并配有一个能够向任意方向驱动滚轮的齿架。滚轮包覆聚氨酯,通过每个单元内部的锥齿轮系统驱动。展开更多
文摘去泛素化酶含OTU结构域蛋白3(OTU domain-containing protein 3,OTUD3)是非常重要的去泛素化酶,在多种疾病模型中存在明显的表达差异。在不同细胞中,OTUD3参与调控凋亡、炎症反应、细胞周期、增殖和侵袭等生物学功能。OTUD3通过调节多种关键底物蛋白的去泛素化,在先天性抗病毒免疫、神经发育、神经系统退行性疾病、肿瘤等生理和病理过程中发挥重要作用。OTUD3在乳腺癌、食管癌、结肠癌、甲状腺乳头状癌中发挥抑癌作用,但在肝癌和肺癌中发挥促癌作用。作为恶性肿瘤的生物标志物,OTUD3在预测、诊断和判断预后方面起重要作用。OTUD3在大部分疾病中的相关分子机制研究仍不够深入,研究OTUD3的调控机制对于深入了解其在免疫和疾病中的作用至关重要,以OTUD3为靶标的药物研发将是今后的研究重点。
基金supported by grants from the ICGEB (International Center for Genetic Engineering and Biotechnology) (CRP/CHN02-01) (SONG Ping)the National Basic Research Program of China (2004CB117400) (SONG Ping)+1 种基金the National Natural Science Foundation of China (30150005 30270675) (SONG Ping)
文摘Zebrafish（Danio rerio） Z-OTU,containing OTU and TUDOR domains,was predicted to be a member of OTU-related protease,a family of the deubiquitylating enzymes（DUBs）.A previous report from our laboratory clearly describes the expression patterns of z-otu mRNA.Here,we characterized the Z-OTU protein during zebrafish oogenesis and early embryogenesis.After prokaryotic expression,the recombinant protein of the OTU domain and GST was purified and injected into rabbits to obtain the polyclonal antibody-anti-Z-OTU,which was used for immunohistochemistry in zebrafish ovaries and embryos.Interestingly,obvious differences existed between the expression patterns of z-otu mRNA and its protein during oogenesis and early embryogenesis.In stage I oocytes,z-otu mRNA was detected in cytoplasm while its protein existed in the germinal vesicle.In addition,its protein was distributed during entire oogenesis,while mRNA was not detected in oocytes at stage IV or mature oocytes.The z-otu mRNA disappeared after midblastula transition（MBT） and its protein gradually decreased after this stage.We inferred that Z-OTU protein,like other OTU-related protease with DUB activity,was required for germinal vesicle breakdown of oocytes during meiosis,germinal vesicle migration,and embryo cleavage maintenance.
文摘瑞士Avancon股份有限公司生产的万向传输单元(Omni-directional Transfer Unit,OTU),可应用在内部物流系统的输送装置上。OTU可用于进料装置、开关、转向器、交叉点、输送器、分拣机等,实现任意方向的输送;或组合成任意长度和宽度的OTU换向台,并以现场总线为接口,建立起一套完整的控制系统。每个OTU都由尺寸为75×75×133毫米的高科技聚合物材料构成,并配有一个能够向任意方向驱动滚轮的齿架。滚轮包覆聚氨酯,通过每个单元内部的锥齿轮系统驱动。
