Dear Editor,Dual base editors(DuBEs)enable the simultaneous introduction of multiple types of nucleotide substitutions using a single guide RNA(sgRNA),thus greatly broadening the product diversity of base editing.Plan...Dear Editor,Dual base editors(DuBEs)enable the simultaneous introduction of multiple types of nucleotide substitutions using a single guide RNA(sgRNA),thus greatly broadening the product diversity of base editing.Plant dual cytosine and adenine base editors(A&CBEs)were developed by fusing an apolipoprotein B mRNA-editing enzyme catalytic polypeptide(APOBEC)/activation-induced cytidine deaminase(AID)-like deaminase and an artificially evolved TadA-8e adenine deaminase to the same or opposite termini of a catalytically impaired Cas9 nickase(Li et al.,2020,2024;Xu et al.,2021;Zhang et al.,2023a,2023b;Zheng et al.,2024).展开更多
基金funded by the National Key Research and Development Program(2022YFF1002803)the National Natural Science Foundation of China(32570484 and 32572441)+2 种基金the Science and Technology Major Project of Anhui Province(Nos.202423m10050002,2023n06020020,and 202423110050063)the Natural Science Foundation of Hefei(HZR2449)the Natural Science Research Project of the Anhui Educational Committee(Nos.2024AH050459,2024AH050424,and 2022AH010056).
文摘Dear Editor,Dual base editors(DuBEs)enable the simultaneous introduction of multiple types of nucleotide substitutions using a single guide RNA(sgRNA),thus greatly broadening the product diversity of base editing.Plant dual cytosine and adenine base editors(A&CBEs)were developed by fusing an apolipoprotein B mRNA-editing enzyme catalytic polypeptide(APOBEC)/activation-induced cytidine deaminase(AID)-like deaminase and an artificially evolved TadA-8e adenine deaminase to the same or opposite termini of a catalytically impaired Cas9 nickase(Li et al.,2020,2024;Xu et al.,2021;Zhang et al.,2023a,2023b;Zheng et al.,2024).
