Synergistically improving the yield and grain quality of rice remains a major breeding challenge.Amino acid transporters play key roles in regulating plant growth and development,but their mechanisms in synergisticall...Synergistically improving the yield and grain quality of rice remains a major breeding challenge.Amino acid transporters play key roles in regulating plant growth and development,but their mechanisms in synergistically regulating yield and quality remain unclear.Here,we report that the plasma membrane-localized transporter OsAAP18,which is more highly expressed in indica than japonica rice,positively correlates with tiller number and yield but negatively with grain width.OsAAP18 transports eight amino acids,including asparagine(Asn),proline(Pro),leucine(Leu),and valine(Val).Its overexpression increases yield through enhanced tillering and grain number per panicle while also improving rice processing and cooking quality.Transcriptome analysis showed that OsAAP18 coordinates grain development and quality formation by regulating the expression of key genes involved in starch and sucrose metabolism,nitrogen metabolism,and plant hormone signaling pathways.These findings establish OsAAP18 as a dual-function regulator that synergistically enhances yield and quality,offering a promising target for rice breeding.展开更多
基金supported by the National Natural Science Foundation of China(Grant Nos.32560065 and 32572249)the Guizhou Provincial Excellent Young Talents Project of Science and Technology,China(Grant No.qiankehepingtairencai-YQK(2023)002)+4 种基金the Guizhou Provincial Science and Technology Projects,China(Grant Nos.qiankehechengguo(2024)General 116 and qiankehejichu-ZK(2022)Key 008)the Key Laboratory of High Quality,High Efficiency,and Yield Enhancement in Grain and Oil Crops,China(Grant No.Qiankehe-Platform ZSYS(2025)037)the Key Laboratory of Functional Agriculture of Guizhou Provincial Department of Education,China(Grant No.Qianjiaoji(2023)007)Guizhou Provincial Modern Agricultural Industry Technology System Construction Special Program(Grant No.GZSDCYJSTX-202602)the Qiandongnan Science and Technology Support Project,China(Grant No.Qiandongnan Kehe Support(2023)07).
文摘Synergistically improving the yield and grain quality of rice remains a major breeding challenge.Amino acid transporters play key roles in regulating plant growth and development,but their mechanisms in synergistically regulating yield and quality remain unclear.Here,we report that the plasma membrane-localized transporter OsAAP18,which is more highly expressed in indica than japonica rice,positively correlates with tiller number and yield but negatively with grain width.OsAAP18 transports eight amino acids,including asparagine(Asn),proline(Pro),leucine(Leu),and valine(Val).Its overexpression increases yield through enhanced tillering and grain number per panicle while also improving rice processing and cooking quality.Transcriptome analysis showed that OsAAP18 coordinates grain development and quality formation by regulating the expression of key genes involved in starch and sucrose metabolism,nitrogen metabolism,and plant hormone signaling pathways.These findings establish OsAAP18 as a dual-function regulator that synergistically enhances yield and quality,offering a promising target for rice breeding.
