A space-based bistatic radar system composed of two space-based radars as the transmitter and the receiver respectively has a wider surveillance region and a better early warning capability for high-speed targets,and ...A space-based bistatic radar system composed of two space-based radars as the transmitter and the receiver respectively has a wider surveillance region and a better early warning capability for high-speed targets,and it can detect focused space targets more flexibly than the monostatic radar system or the ground-based radar system.However,the target echo signal is more difficult to process due to the high-speed motion of both space-based radars and space targets.To be specific,it will encounter the problems of Range Cell Migration(RCM)and Doppler Frequency Migration(DFM),which degrade the long-time coherent integration performance for target detection and localization inevitably.To solve this problem,a novel target detection method based on an improved Gram Schmidt(GS)-orthogonalization Orthogonal Matching Pursuit(OMP)algorithm is proposed in this paper.First,the echo model for bistatic space-based radar is constructed and the conditions for RCM and DFM are analyzed.Then,the proposed GS-orthogonalization OMP method is applied to estimate the equivalent motion parameters of space targets.Thereafter,the RCM and DFM are corrected by the compensation function correlated with the estimated motion parameters.Finally,coherent integration can be achieved by performing the Fast Fourier Transform(FFT)operation along the slow time direction on compensated echo signal.Numerical simulations and real raw data results validate that the proposed GS-orthogonalization OMP algorithm achieves better motion parameter estimation performance and higher detection probability for space targets detection.展开更多
Gram-Schmidt orthogonalization algorithm is an interesting theme in the field of adaptive beam-forming and filtering as a fast algorithm. However, a key problem associated with this algorithm is that the number of ort...Gram-Schmidt orthogonalization algorithm is an interesting theme in the field of adaptive beam-forming and filtering as a fast algorithm. However, a key problem associated with this algorithm is that the number of orthogonalization, namely, the dimensions of interference subspace, is required to know prior. In this paper we derive a threshold and adopt it to detect the number of orthogonalization in the procedure of Gram-Schmidt(GS) orthogonalization decomposition, and this detection approach is simpler and faster than the approach based on eigenanalysis. Finally, computer simulation results were presented too.展开更多
UE-Brzier （unified and extended Brzier） basis is the unified form of Brzier-like bases, including polynomial Brzier basis, trigonometric polynomial and hyperbolic polynomial Brzier basis. Similar to the original Brz...UE-Brzier （unified and extended Brzier） basis is the unified form of Brzier-like bases, including polynomial Brzier basis, trigonometric polynomial and hyperbolic polynomial Brzier basis. Similar to the original Brzier-like bases, UE-Brzier basis func-tions are not orthogonal. In this paper, a group of orthogonal basis is constructed based on UE-Brzier basis. The transformation matrices between UE-Brzier basis and the proposed orthogonal basis are also solved.展开更多
Two optimal orthogonalization processes are devised toorthogonalize,possibly approximately,the columns of a very large and possiblysparse matrix A∈C^(n×k).Algorithmically the aim is,at each step,to optimallydecr...Two optimal orthogonalization processes are devised toorthogonalize,possibly approximately,the columns of a very large and possiblysparse matrix A∈C^(n×k).Algorithmically the aim is,at each step,to optimallydecrease nonorthogonality of all the columns of A.One process relies on using translated small rank corrections.Another is a polynomial orthogonalization process forperforming the Löwdin orthogonalization.The steps rely on using iterative methods combined,preferably,with preconditioning which can have a dramatic effect on how fast thenonorthogonality decreases.The speed of orthogonalization depends on howbunched the singular values of A are,modulo the number of steps taken.These methods put the steps of the Gram-Schmidt orthogonalizationprocess into perspective regardingtheir(lack of)optimality.The constructions are entirely operatortheoretic and can be extended to infinite dimensional Hilbert spaces.展开更多
Due to water conflicts and allocation in the Lancang-Mekong River Basin(LMRB),the spatio-temporal differentiation of total water resources and the natural-human influence need to be clarified.This work investigated LM...Due to water conflicts and allocation in the Lancang-Mekong River Basin(LMRB),the spatio-temporal differentiation of total water resources and the natural-human influence need to be clarified.This work investigated LMRB's terrestrial water storage anomaly(TWSA)and its spatio-temporal dynamics during 2002–2020.Considering the effects of natural factors and human activities,the respective contributions of climate variability and human activities to terrestrial water storage change(TWSC)were separated.Results showed that:(1)LMRB's TWSA decreased by 0.3158 cm/a.(2)TWSA showed a gradual increase in distribution from southwest of MRB to middle LMRB and from northeast of LRB to middle LMRB.TWSA positively changed in Myanmar while slightly changed in Laos and China.It negatively changed in Vietnam,Thailand and Cambodia.(3)TWSA components decreased in a descending order of soil moisture,groundwater and precipitation.(4)Natural factors had a substantial and spatial differentiated influence on TWSA over the LMRB.(5)Climate variability contributed 79%of TWSC in the LMRB while human activities contributed 21%with an increasing impact after 2008.The TWSC of upstream basin countries was found to be controlled by climate variability while Vietnam and Cambodia's TWSC has been controlled by human activities since 2012.展开更多
This study aimed to address the challenges of solid waste utilization,cost reduction,and carbon reduction in the treatment of deep-dredged soil at Xuwei Port in Lianyungang city of China.Past research in this area was...This study aimed to address the challenges of solid waste utilization,cost reduction,and carbon reduction in the treatment of deep-dredged soil at Xuwei Port in Lianyungang city of China.Past research in this area was limited.Therefore,a curing agent made from powdered shells was used to solidify the dredged soil in situ.We employed laboratory orthogonal tests to investigate the physical and mechanical properties of the powdered shell-based curing agent.Data was collected by conducting experiments to assess the role of powdered shells in the curing process and to determine the optimal ratios of powdered shells to solidified soil for different purposes.The development of strength in solidified soil was studied in both seawater and pure water conditions.The study revealed that the strength of the solidified soil was influenced by the substitution rate of powdered shells and their interaction with cement.Higher cement content had a positive effect on strength.For high-strength solidified soil,the recommended ratio of wet soil:cement:lime:powdered shells were 100:16:4:4,while for low-strength solidified soil,the recommended ratio was 100:5.4:2.4:0.6.Seawater,under appropriate conditions,improved short-term strength by promoting the formation of expansive ettringite minerals that contributed to cementation and precipitation.These findings suggest that the combination of cement and powdered shells is synergistic,positively affecting the strength of solidified soil.The recommended ratios provide practical guidance for achieving desired strength levels while considering factors such as cost and carbon emissions.The role of seawater in enhancing short-term strength through crystal formation is noteworthy and can be advantageous for certain applications.In conclusion,this research demonstrates the potential of using a powdered shell-based curing agent for solidifying dredged soil in an environmentally friendly and cost-effective manner.The recommended ratios for different strength requirements offer valuable insights for practical applications in the field of soil treatment,contributing to sustainable and efficient solutions for soil management.展开更多
The injection of bone cement is a promising surgical intervention for the treatment of osteoporosis.The aim of this study was to formulate a novel injectable bioactive bone cement to adress such medical problems.The b...The injection of bone cement is a promising surgical intervention for the treatment of osteoporosis.The aim of this study was to formulate a novel injectable bioactive bone cement to adress such medical problems.The bone cement primarily consists of tricalcium phosphate(β-TCP),konjac glucomannan(KGM),and hydroxyapatite whisker(HAw).An orthogonal experiment was designed to generate multiple sets of new composite calcium phosphate cement(NCPC)samples,and their setting times were measured.The in vitro compatibility of the new bone cement was assessed through relative cell proliferation rate(RGR)and in vitro cell growth experiments.Mechanical strength and porosity tests were conducted for each group of bone cement,and cross-sectional morphology was observed.The results demonstrate that the bone cement exhibits favorable properties such as self-curing,mechanical robustness,and resistance to collapse.The optimum formulation involves a doping ratio of 5/15(wt%)HAw and HA,an additional amount of 1.2wt%KGM,and a liquid citric acid concentration of 2wt%.Porosity tests confirmed that the material has high compressive strength and a favorable porosity of 27%,creating conducive conditions for cell growth,proliferation,and material degradation.Moreover,in vitro cell culture experiments revealed excellent biocompatibility of the material.Consequently,the developed NCPC emerges as a potential candidate material for applications of bone implantation.展开更多
Extensive spatiotemporal analyses of long-trend surface ozone in the Yangtze River Delta(YRD)region and itsmeteorology-related and emission-related have not been systematically analyzed.In this study,by using 8-year-l...Extensive spatiotemporal analyses of long-trend surface ozone in the Yangtze River Delta(YRD)region and itsmeteorology-related and emission-related have not been systematically analyzed.In this study,by using 8-year-long(2015–2022)surface ozone observation data,we attempted to reveal the variation ofmultiple timescale components using the Kolmogorov–Zurbenko filter,and the effects of meteorology and emissions were quantitatively isolated using multiple linear regression with meteorological variables.The results showed that the short-term,seasonal,and long-term components accounted for daily maximum 8-hr average O_(3)(O_(3–8)hr)concentration,46.4%,45.9%,and 1.0%,respectively.The meteorological impacts account for an average of 71.8%of O_(3–8)hr,and the YRD’s eastern and northern sections aremeteorology-sensitive areas.Based on statistical analysis technology with empirical orthogonal function,the contribution of meteorology,local emission,and transport in the long-term component of O_(3–8)hr were 0.21%,0.12%,and 0.6%,respectively.The spatiotemporal analysis indicated that a distinct decreasing spatial pattern could be observed from coastal cities towards the northwest,influenced by the monsoon and synoptic conditions.The central urban agglomeration north and south of the YRD was particularly susceptible to local pollution.Among the cities studied,Shanghai,Anqing,and Xuancheng,located at similar latitudes,were significantly impacted by atmospheric transmission—the contribution of Shanghai,the maximum accounting for 3.6%.展开更多
The minimal widths of three bounded subsets of the unit sphere associated to a unit vector in a normed linear space are studied,and three related geometric constants are introduced.New characterizations of inner produ...The minimal widths of three bounded subsets of the unit sphere associated to a unit vector in a normed linear space are studied,and three related geometric constants are introduced.New characterizations of inner product spaces are also presented.From the perspective of minimal width,strongε-symmetry of Birkhoff orthogonality is introduced,and its relation toε-symmetry of Birkhoff orthogonality is shown.Unlike most of the existing parameters of the underlying space,these new constants are full dimensional in nature.展开更多
[Objectives]To optimize the optimal extraction process of Qingdu Jianpi Mixture.[Methods]Taking water addition ratio,extraction time and extraction times as process investigation factors,psoralen content,astilbin cont...[Objectives]To optimize the optimal extraction process of Qingdu Jianpi Mixture.[Methods]Taking water addition ratio,extraction time and extraction times as process investigation factors,psoralen content,astilbin content and dry extract yield as evaluation indicators,the main influencing factors and level range of the extraction process of Qingdu Jianpi Mixture were determined on the basis of single factor test method,and the optimal weight coefficient was screened by AHP-entropy method mixed with weighting method.Combined with L_(9)(3^(4))orthogonal experiment,the best extraction process was obtained.At the same time,thin-layer chromatographic identification was used to identify Ficus simplicissima Lour.and Smilax glabra Roxb.in the medicinal liquid.[Results]The best extraction process:add 1:12 water to the prescription decoction pieces,extract under reflux for 2 times,1.5 h per time,and combine the filtrate to 250 mL.Thin layer chromatography analysis showed that the spots of Ficus simplicissima Lour.and Smilax glabra Roxb.in the medicinal solution were the same as those of reference substances at the corresponding positions,and the negative control had no interference.[Conclusions]The experimental method is reasonable and feasible,and the process is reliable,which can provide experimental reference for the subsequent application of in-hospital preparations and research and development of Qingdu Jianpi Mixture.展开更多
Molecular recognition of bioreceptors and enzymes relies on orthogonal interactions with small molecules within their cavity. To date, Chinese scientists have developed three types of strategies for introducing active...Molecular recognition of bioreceptors and enzymes relies on orthogonal interactions with small molecules within their cavity. To date, Chinese scientists have developed three types of strategies for introducing active sites inside the cavity of macrocyclic arenes to better mimic molecular recognition of bioreceptors and enzymes.The editorial aims to enlighten scientists in this field when they develop novel macrocycles for molecular recognition, supramolecular assembly, and applications.展开更多
Used extensively in the aerospace industry,the superalloy Inconel 718 is deemed hard to cut because of its rapid work hardening,low thermal conductivity,and excessive shear strength.Proposed here is patterning Inconel...Used extensively in the aerospace industry,the superalloy Inconel 718 is deemed hard to cut because of its rapid work hardening,low thermal conductivity,and excessive shear strength.Proposed here is patterning Inconel 718 with micro-dimples using electrochemical machining and electrical discharge machining prior to conventional cutting.How this micro-dimple patterning influences the machinability of Inconel 718 is analyzed via orthogonal cutting experiments,and with the optimal parameters,the cutting temperature is lowered by 45.5%and the cutting forces are reduced significantly,i.e.,the tangential cutting force and the thrust force are reduced by 61.1% and 47.1%,respectively.The predictions of finite-element simulations agree with experimental observations,and it is concluded that dimpled Inconel 718 offers significantly improved tool wear and surface integrity.展开更多
To investigate the influence of W and Al on the microstructure and mechanical properties of a high-W superalloy,the Thermo-Calc calculation was utilized to simulate the microstructure with various W and Al contents.Th...To investigate the influence of W and Al on the microstructure and mechanical properties of a high-W superalloy,the Thermo-Calc calculation was utilized to simulate the microstructure with various W and Al contents.The results indicated that the concentration of W and Al exceeded 15.7 wt%and 5.9 wt%,respectively,the abnormal tungsten-richα-W phase would precipitate.Compared with the results of orthogonal experiment,the precipitation ofα-W phase is consistent with thermodynamic calculation results.The presence of Al not only influenced the precipitation ofα-W phase but also impacted the eutectic content and theγʹ-size,both of which showed an increase with higher Al concentrations.Excessive W and Al contents promoted the precipitation ofα-W phase,escalating the site of crack nucleation,and ultimately decreasing the plasticity.In the process of creep deformation(975℃/235 MPa),the raftedγ'phases were more continuous with increasing W contents,which increased the difficulty of dislocation climbing.As Al content increased,the density of interfacial dislocation network increased.The dislocations were entangled with each other,and the hindrance of dislocation movement was enhanced,which improved the stress rupture life.However,the precipitation of the hard and brittleα-W phase was attributed to the excessive W and Al,which increased the tendency of crack formation and significantly diminished the stress rupture life.The alloy exhibited the highest stress rupture life of 110.46 h when the W and Al contents were 15.7 wt%and 5.9 wt%,respectively.展开更多
Wire⁃Electrical Discharge Machining(WEDM)process can create any complex contour in any conducting material,regardless of its strength or hardness,with higher accuracy.Present experimental investigation aims to determi...Wire⁃Electrical Discharge Machining(WEDM)process can create any complex contour in any conducting material,regardless of its strength or hardness,with higher accuracy.Present experimental investigation aims to determine suitable input process/machining parameters,e.g.pulse on time(TON),peak current(IP),wire feed rate(WF),and pulse off time(TOFF),for optimizing the process performances,namely,cutting rate,kerf width,average roughness value of the machined surface,micro hardness,and surface crack density.Since four input parameters are considered in the present investigation,and each parameter is assumed to vary at three different levels(i.e.low,medium,high),the Taguchi L9 Orthogonal Array(OA)design approach has been used for the experimental purpose to enhance the process effiency.Similarly,a simple and popular multiresponse optimization approach,namely Grey Relational Analysis(GRA),is used to optimize five performance characteristics(output responses)simultaneously.The optimum process variables obtained are:TON of 110μs,TOFF of 40μs,IP of 10 A,and WF of 6 mm/min.These optimum process variables are validated with confirmatory experiment.The relative impact of input variables is determined using the Analysis of Variance(ANOVA)technique.Finally,correlations between individual outputs with different input parameters are established.This work will be helpful for industry personnel to use this machining process in a techno⁃economic way.展开更多
Seismic wavefields propagate through three-dimensional(3D)space,and their precise characterization is crucial for understanding subsurface structures.Traditional 2D algorithms,due to their limitations,are insufficient...Seismic wavefields propagate through three-dimensional(3D)space,and their precise characterization is crucial for understanding subsurface structures.Traditional 2D algorithms,due to their limitations,are insufficient to fully represent three-dimensional wavefields.The classic 3D Radon transform algorithm assumes that the wavefield's propagation characteristics are consistent in all directions,which often does not hold true in complex underground media.To address this issue,we present an improved 3D three-parameter Radon algorithm that considers the wavefield variation with azimuth and provides a more accurate wavefield description.However,introducing new parameters to describe the azimuthal varia-tion also poses computational challenges.The new Radon transform operator involves five variables and cannot be simply decomposed into small matrices for efficient computation;instead,it requires large matrix multiplication and inversion operations,significantly increasing the computational load.To overcome this challenge,we have integrated the curvature and frequency parameters,simplifying all frequency operators to the same,thereby significantly improving computation efficiency.Furthermore,existing transform algorithms neglect the lateral variation of seismic amplitudes,leading to discrepancies between the estimated multiples and those in the data.To enhance the amplitude preservation of the algorithm,we employ orthogonal polynomial fitting to capture the amplitude spatial variation in 3D seismic data.Combining these improvements,we propose a fast,amplitude-preserving,3D three-parameter Radon transform algorithm.This algorithm not only enhances computational efficiency while maintaining the original wavefield characteristics,but also improves the representation of seismic data by increasing amplitude fidelity.We validated the algorithm in multiple attenuation using both synthetic and real seismic data.The results demonstrate that the new algorithm significantly improves both accuracy and computational efficiency,providing an effective tool for analyzing seismic wavefields in complex subsurface structures.展开更多
Nitrogen oxides(NO_(x))and particulate matter(PM)present significant risks to both human health and environmental sustainability.The Integrated Dust Removal and Denitrification Technology(DRDt)offers a more efficient ...Nitrogen oxides(NO_(x))and particulate matter(PM)present significant risks to both human health and environmental sustainability.The Integrated Dust Removal and Denitrification Technology(DRDt)offers a more efficient and cost-effective solution for achieving ultralow industrial flue gas emissions;however,its effectiveness is undermined by low catalyst load rates and poor stability in filter materials.This study addresses these limitations by modifying conventional PTFE filter media(PTFE-Tim)through the incorporation of sodium alginate(SA)and dopamine(DA)as modifiers,resulting in two new filter materials:PTFE-SA-MOF and PTFE-DA-MOF.By optimizing the parameters of an orthogonal experimental design,we identified the ideal preparation conditions for these composite materials.The addition of SA and DA enhanced the bonding between the catalyst(Mn-Cu-MOF)crystal particles and the PTFE fibers through mechanisms such as ion exchange,hydrogen bonding,and adhesion.Consequently,the catalyst loading rate and stability of the DRDt filters were significantly improved.Specifically,the PTFE-SA-MOF and PTFE-DA-MOF filters achieved high catalyst loading rates of 15.97% and 15.86%,these values represent improvements of 2.53 and 2.51 times,while maintaining excellent stability,with mass retention rates of 98.64% and 98.27%,respectively,over the conventional PTFE-Tim filter.展开更多
Recent advancements in computational and database technologies have led to the exponential growth of large-scale medical datasets,significantly increasing data complexity and dimensionality in medical diagnostics.Effi...Recent advancements in computational and database technologies have led to the exponential growth of large-scale medical datasets,significantly increasing data complexity and dimensionality in medical diagnostics.Efficient feature selection methods are critical for improving diagnostic accuracy,reducing computational costs,and enhancing the interpretability of predictive models.Particle Swarm Optimization(PSO),a widely used metaheuristic inspired by swarm intelligence,has shown considerable promise in feature selection tasks.However,conventional PSO often suffers from premature convergence and limited exploration capabilities,particularly in high-dimensional spaces.To overcome these limitations,this study proposes an enhanced PSO framework incorporating Orthogonal Initializa-tion and a Crossover Operator(OrPSOC).Orthogonal Initialization ensures a diverse and uniformly distributed initial particle population,substantially improving the algorithm’s exploration capability.The Crossover Operator,inspired by genetic algorithms,introduces additional diversity during the search process,effectively mitigating premature convergence and enhancing global search performance.The effectiveness of OrPSOC was rigorously evaluated on three benchmark medical datasets—Colon,Leukemia,and Prostate Tumor.Comparative analyses were conducted against traditional filter-based methods,including Fast Clustering-Based Feature Selection Technique(Fast-C),Minimum Redundancy Maximum Relevance(MinRedMaxRel),and Five-Way Joint Mutual Information(FJMI),as well as prominent metaheuristic algorithms such as standard PSO,Ant Colony Optimization(ACO),Comprehensive Learning Gravitational Search Algorithm(CLGSA),and Fuzzy-Based CLGSA(FCLGSA).Experimental results demonstrated that OrPSOC consistently outperformed these existing methods in terms of classification accuracy,computational efficiency,and result stability,achieving significant improvements even with fewer selected features.Additionally,a sensitivity analysis of the crossover parameter provided valuable insights into parameter tuning and its impact on model performance.These findings highlight the superiority and robustness of the proposed OrPSOC approach for feature selection in medical diagnostic applications and underscore its potential for broader adoption in various high-dimensional,data-driven fields.展开更多
Silicon Carbide (SiC) wafers have been widely used in micro- and nano-devices due to their excellent optical and material properties. However, polishing SiC wafers has been challenging and inefficient, tending to caus...Silicon Carbide (SiC) wafers have been widely used in micro- and nano-devices due to their excellent optical and material properties. However, polishing SiC wafers has been challenging and inefficient, tending to cause significant surface crack and subsurface damage. This work proposed modifying SiC surface properties by ion implantation to improve machining efficiency, suppress surface crack, and reduce damage. High-energy ion implantation disrupted the SiC crystal lattice, reducing hardness and elastic modulus while increasing brittle-ductile transition depth, thus changing the removal mode from brittle fracture to plastic removal. Theoretical models of material removal rate and surface roughness were established for abrasive polishing of the SiC wafers. Polishing experiments were conducted on ion-implanted, modified SiC samples. The improvement mechanisms of ion implantation on surface damage, removal rate, morphology, and residual stress were investigated. The effect of ion implantation on the polished surface quality of SiC was investigated through orthogonal experiments. The results showed that ion implantation can significantly improve the average material removal rate of the SiC samples. Additionally, the ion-implanted samples had exhibited remarkable reductions in surface roughness, surface damage, and tensile residual stress.展开更多
In this paper,we introduce a new geometric constant R_(X)(κ)based on isosceles orthogonality.First,we explore some basic properties of this new constant and then provide several examples to estimate its exact values ...In this paper,we introduce a new geometric constant R_(X)(κ)based on isosceles orthogonality.First,we explore some basic properties of this new constant and then provide several examples to estimate its exact values in certain specific Banach spaces.Next,we investigate the relationships between this new constant and other classical constants.Specifically,we establish an inequality relationship between it and the J(X)constant,as well as an identity relationship between it and theρX(t)constant.Furthermore,we characterize some geometric properties of Banach spaces by means of this new constant.Finally,by restricting the above-mentioned constant to the unit sphere,we introduce another new constant,calculate its upper and lower bounds,and present a relevant example.展开更多
At present,the polymerase chain reaction(PCR)amplification-based file retrieval method is the mostcommonly used and effective means of DNA file retrieval.The number of orthogonal primers limitsthe number of files that...At present,the polymerase chain reaction(PCR)amplification-based file retrieval method is the mostcommonly used and effective means of DNA file retrieval.The number of orthogonal primers limitsthe number of files that can be accurately accessed,which in turn affects the density in a single oligo poolof digital DNA storage.In this paper,a multi-mode DNA sequence design method based on PCR file retrie-val in a single oligonucleotide pool is proposed for high-capacity DNA data storage.Firstly,by analyzingthe maximum number of orthogonal primers at each predicted primer length,it was found that the rela-tionship between primer length and the maximum available primer number does not increase linearly,and the maximum number of orthogonal primers is on the order of 10^(4).Next,this paper analyzes themaximum address space capacity of DNA sequences with different types of primer binding sites for filemapping.In the case where the capacity of the primer library is R(where R is even),the number ofaddress spaces that can be mapped by the single-primer DNA sequence design scheme proposed in thispaper is four times that of the previous one,and the two-level primer DNA sequence design scheme can reach [R/2·(R/2-1)]^(2)times.Finally,a multi-mode DNA sequence generation method is designed based onthe number of files to be stored in the oligonucleotide pool,in order to meet the requirements of the ran-dom retrieval of target files in an oligonucleotide pool with large-scale file numbers.The performance ofthe primers generated by the orthogonal primer library generator proposed in this paper is verified,andthe average Gibbs free energy of the most stable heterodimer formed between the orthogonal primersproduced is−1 kcal·(mol·L^(−1))^(−1)(1 kcal=4.184 kJ).At the same time,by selectively PCR-amplifying theDNA sequences of the two-level primer binding sites for random access,the target sequence can be accu-rately read with a minimum of 10^(3) reads,when the primer binding site sequences at different positionsare mutually different.This paper provides a pipeline for orthogonal primer library generation and multi-mode mapping schemes between files and primers,which can help achieve precise random access to filesin large-scale DNA oligo pools.展开更多
文摘A space-based bistatic radar system composed of two space-based radars as the transmitter and the receiver respectively has a wider surveillance region and a better early warning capability for high-speed targets,and it can detect focused space targets more flexibly than the monostatic radar system or the ground-based radar system.However,the target echo signal is more difficult to process due to the high-speed motion of both space-based radars and space targets.To be specific,it will encounter the problems of Range Cell Migration(RCM)and Doppler Frequency Migration(DFM),which degrade the long-time coherent integration performance for target detection and localization inevitably.To solve this problem,a novel target detection method based on an improved Gram Schmidt(GS)-orthogonalization Orthogonal Matching Pursuit(OMP)algorithm is proposed in this paper.First,the echo model for bistatic space-based radar is constructed and the conditions for RCM and DFM are analyzed.Then,the proposed GS-orthogonalization OMP method is applied to estimate the equivalent motion parameters of space targets.Thereafter,the RCM and DFM are corrected by the compensation function correlated with the estimated motion parameters.Finally,coherent integration can be achieved by performing the Fast Fourier Transform(FFT)operation along the slow time direction on compensated echo signal.Numerical simulations and real raw data results validate that the proposed GS-orthogonalization OMP algorithm achieves better motion parameter estimation performance and higher detection probability for space targets detection.
文摘Gram-Schmidt orthogonalization algorithm is an interesting theme in the field of adaptive beam-forming and filtering as a fast algorithm. However, a key problem associated with this algorithm is that the number of orthogonalization, namely, the dimensions of interference subspace, is required to know prior. In this paper we derive a threshold and adopt it to detect the number of orthogonalization in the procedure of Gram-Schmidt(GS) orthogonalization decomposition, and this detection approach is simpler and faster than the approach based on eigenanalysis. Finally, computer simulation results were presented too.
基金Supported by National Science Foundation of China(No.60904070,61272032)the Natural Science Foundation of Zhejiang Province(No.LY12F02002,Y1111101)
文摘UE-Brzier （unified and extended Brzier） basis is the unified form of Brzier-like bases, including polynomial Brzier basis, trigonometric polynomial and hyperbolic polynomial Brzier basis. Similar to the original Brzier-like bases, UE-Brzier basis func-tions are not orthogonal. In this paper, a group of orthogonal basis is constructed based on UE-Brzier basis. The transformation matrices between UE-Brzier basis and the proposed orthogonal basis are also solved.
基金supported by the Academy of Finland(Grant No.288641)。
文摘Two optimal orthogonalization processes are devised toorthogonalize,possibly approximately,the columns of a very large and possiblysparse matrix A∈C^(n×k).Algorithmically the aim is,at each step,to optimallydecrease nonorthogonality of all the columns of A.One process relies on using translated small rank corrections.Another is a polynomial orthogonalization process forperforming the Löwdin orthogonalization.The steps rely on using iterative methods combined,preferably,with preconditioning which can have a dramatic effect on how fast thenonorthogonality decreases.The speed of orthogonalization depends on howbunched the singular values of A are,modulo the number of steps taken.These methods put the steps of the Gram-Schmidt orthogonalizationprocess into perspective regardingtheir(lack of)optimality.The constructions are entirely operatortheoretic and can be extended to infinite dimensional Hilbert spaces.
基金National Natural Science Foundation of China,No.42161006Yunnan Fundamental Research Projects No.202201AT070094,No.202301BF070001-004+1 种基金Special Project for High-level Talents of Yunnan Province for Young Top Talents,No.C6213001159European Research Council(ERC)Starting-Grant STORIES,No.101040939。
文摘Due to water conflicts and allocation in the Lancang-Mekong River Basin(LMRB),the spatio-temporal differentiation of total water resources and the natural-human influence need to be clarified.This work investigated LMRB's terrestrial water storage anomaly(TWSA)and its spatio-temporal dynamics during 2002–2020.Considering the effects of natural factors and human activities,the respective contributions of climate variability and human activities to terrestrial water storage change(TWSC)were separated.Results showed that:(1)LMRB's TWSA decreased by 0.3158 cm/a.(2)TWSA showed a gradual increase in distribution from southwest of MRB to middle LMRB and from northeast of LRB to middle LMRB.TWSA positively changed in Myanmar while slightly changed in Laos and China.It negatively changed in Vietnam,Thailand and Cambodia.(3)TWSA components decreased in a descending order of soil moisture,groundwater and precipitation.(4)Natural factors had a substantial and spatial differentiated influence on TWSA over the LMRB.(5)Climate variability contributed 79%of TWSC in the LMRB while human activities contributed 21%with an increasing impact after 2008.The TWSC of upstream basin countries was found to be controlled by climate variability while Vietnam and Cambodia's TWSC has been controlled by human activities since 2012.
基金Funded by the Science and Technology Project of Jiangsu Provincial Transportation Department(No.2022Y13)。
文摘This study aimed to address the challenges of solid waste utilization,cost reduction,and carbon reduction in the treatment of deep-dredged soil at Xuwei Port in Lianyungang city of China.Past research in this area was limited.Therefore,a curing agent made from powdered shells was used to solidify the dredged soil in situ.We employed laboratory orthogonal tests to investigate the physical and mechanical properties of the powdered shell-based curing agent.Data was collected by conducting experiments to assess the role of powdered shells in the curing process and to determine the optimal ratios of powdered shells to solidified soil for different purposes.The development of strength in solidified soil was studied in both seawater and pure water conditions.The study revealed that the strength of the solidified soil was influenced by the substitution rate of powdered shells and their interaction with cement.Higher cement content had a positive effect on strength.For high-strength solidified soil,the recommended ratio of wet soil:cement:lime:powdered shells were 100:16:4:4,while for low-strength solidified soil,the recommended ratio was 100:5.4:2.4:0.6.Seawater,under appropriate conditions,improved short-term strength by promoting the formation of expansive ettringite minerals that contributed to cementation and precipitation.These findings suggest that the combination of cement and powdered shells is synergistic,positively affecting the strength of solidified soil.The recommended ratios provide practical guidance for achieving desired strength levels while considering factors such as cost and carbon emissions.The role of seawater in enhancing short-term strength through crystal formation is noteworthy and can be advantageous for certain applications.In conclusion,this research demonstrates the potential of using a powdered shell-based curing agent for solidifying dredged soil in an environmentally friendly and cost-effective manner.The recommended ratios for different strength requirements offer valuable insights for practical applications in the field of soil treatment,contributing to sustainable and efficient solutions for soil management.
文摘The injection of bone cement is a promising surgical intervention for the treatment of osteoporosis.The aim of this study was to formulate a novel injectable bioactive bone cement to adress such medical problems.The bone cement primarily consists of tricalcium phosphate(β-TCP),konjac glucomannan(KGM),and hydroxyapatite whisker(HAw).An orthogonal experiment was designed to generate multiple sets of new composite calcium phosphate cement(NCPC)samples,and their setting times were measured.The in vitro compatibility of the new bone cement was assessed through relative cell proliferation rate(RGR)and in vitro cell growth experiments.Mechanical strength and porosity tests were conducted for each group of bone cement,and cross-sectional morphology was observed.The results demonstrate that the bone cement exhibits favorable properties such as self-curing,mechanical robustness,and resistance to collapse.The optimum formulation involves a doping ratio of 5/15(wt%)HAw and HA,an additional amount of 1.2wt%KGM,and a liquid citric acid concentration of 2wt%.Porosity tests confirmed that the material has high compressive strength and a favorable porosity of 27%,creating conducive conditions for cell growth,proliferation,and material degradation.Moreover,in vitro cell culture experiments revealed excellent biocompatibility of the material.Consequently,the developed NCPC emerges as a potential candidate material for applications of bone implantation.
基金supported by the National Natural Science Foundation of China(No.42075177)the National Key Research and Development Program of China(No.2017YFC0210003).
文摘Extensive spatiotemporal analyses of long-trend surface ozone in the Yangtze River Delta(YRD)region and itsmeteorology-related and emission-related have not been systematically analyzed.In this study,by using 8-year-long(2015–2022)surface ozone observation data,we attempted to reveal the variation ofmultiple timescale components using the Kolmogorov–Zurbenko filter,and the effects of meteorology and emissions were quantitatively isolated using multiple linear regression with meteorological variables.The results showed that the short-term,seasonal,and long-term components accounted for daily maximum 8-hr average O_(3)(O_(3–8)hr)concentration,46.4%,45.9%,and 1.0%,respectively.The meteorological impacts account for an average of 71.8%of O_(3–8)hr,and the YRD’s eastern and northern sections aremeteorology-sensitive areas.Based on statistical analysis technology with empirical orthogonal function,the contribution of meteorology,local emission,and transport in the long-term component of O_(3–8)hr were 0.21%,0.12%,and 0.6%,respectively.The spatiotemporal analysis indicated that a distinct decreasing spatial pattern could be observed from coastal cities towards the northwest,influenced by the monsoon and synoptic conditions.The central urban agglomeration north and south of the YRD was particularly susceptible to local pollution.Among the cities studied,Shanghai,Anqing,and Xuancheng,located at similar latitudes,were significantly impacted by atmospheric transmission—the contribution of Shanghai,the maximum accounting for 3.6%.
基金supported by the National Natural Science Foundation of China(12071444,12201581)the Fundamental Research Program of Shanxi Province of China(202103021223191).
文摘The minimal widths of three bounded subsets of the unit sphere associated to a unit vector in a normed linear space are studied,and three related geometric constants are introduced.New characterizations of inner product spaces are also presented.From the perspective of minimal width,strongε-symmetry of Birkhoff orthogonality is introduced,and its relation toε-symmetry of Birkhoff orthogonality is shown.Unlike most of the existing parameters of the underlying space,these new constants are full dimensional in nature.
基金Supported by Huang Ruisong's National Famous Old Traditional Chinese Medicine Expert Inheritance Studio Construction Project[GuoZhongYiYaoRenJiaoHan(2022)75]Hospital Pharmacy Research Project of Guangxi Pharmaceutical Association(GXYXH-202404)+4 种基金2024 Youth Science Fund Project of International Zhuang Medical Hospital(2024GZYJKT005)High-level Traditional Chinese Medicine Key Discipline Construction Project of National Administration of Traditional Chinese Medicine(ZYYZDXK-2023165)National Old Pharmaceutical Workers Inheritance Studio Construction Project of National Administration of Traditional Chinese Medicine[GuoZhongYiYaoRenJiaoHan(2024)255]Talent Cultivation Project-"Young Crop Project"of International Zhuang Medical Hospital Affiliated to Guangxi University of Chinese Medicine(2022001)Guangxi Traditional Chinese Medicine Multidisciplinary Innovation Team Project(GZKJ2309).
文摘[Objectives]To optimize the optimal extraction process of Qingdu Jianpi Mixture.[Methods]Taking water addition ratio,extraction time and extraction times as process investigation factors,psoralen content,astilbin content and dry extract yield as evaluation indicators,the main influencing factors and level range of the extraction process of Qingdu Jianpi Mixture were determined on the basis of single factor test method,and the optimal weight coefficient was screened by AHP-entropy method mixed with weighting method.Combined with L_(9)(3^(4))orthogonal experiment,the best extraction process was obtained.At the same time,thin-layer chromatographic identification was used to identify Ficus simplicissima Lour.and Smilax glabra Roxb.in the medicinal liquid.[Results]The best extraction process:add 1:12 water to the prescription decoction pieces,extract under reflux for 2 times,1.5 h per time,and combine the filtrate to 250 mL.Thin layer chromatography analysis showed that the spots of Ficus simplicissima Lour.and Smilax glabra Roxb.in the medicinal solution were the same as those of reference substances at the corresponding positions,and the negative control had no interference.[Conclusions]The experimental method is reasonable and feasible,and the process is reliable,which can provide experimental reference for the subsequent application of in-hospital preparations and research and development of Qingdu Jianpi Mixture.
文摘Molecular recognition of bioreceptors and enzymes relies on orthogonal interactions with small molecules within their cavity. To date, Chinese scientists have developed three types of strategies for introducing active sites inside the cavity of macrocyclic arenes to better mimic molecular recognition of bioreceptors and enzymes.The editorial aims to enlighten scientists in this field when they develop novel macrocycles for molecular recognition, supramolecular assembly, and applications.
基金This work was supported financially by Portescap India Pvt.Ltd. via its CSR fund.
文摘Used extensively in the aerospace industry,the superalloy Inconel 718 is deemed hard to cut because of its rapid work hardening,low thermal conductivity,and excessive shear strength.Proposed here is patterning Inconel 718 with micro-dimples using electrochemical machining and electrical discharge machining prior to conventional cutting.How this micro-dimple patterning influences the machinability of Inconel 718 is analyzed via orthogonal cutting experiments,and with the optimal parameters,the cutting temperature is lowered by 45.5%and the cutting forces are reduced significantly,i.e.,the tangential cutting force and the thrust force are reduced by 61.1% and 47.1%,respectively.The predictions of finite-element simulations agree with experimental observations,and it is concluded that dimpled Inconel 718 offers significantly improved tool wear and surface integrity.
基金financial support received from the National Key Research and Development Program of China(2022YFB3705000)the National Natural Science Foundation of China(No.52303394)the Liaoning Provincial Natural Science Foundation(No.2023-BS-015).
文摘To investigate the influence of W and Al on the microstructure and mechanical properties of a high-W superalloy,the Thermo-Calc calculation was utilized to simulate the microstructure with various W and Al contents.The results indicated that the concentration of W and Al exceeded 15.7 wt%and 5.9 wt%,respectively,the abnormal tungsten-richα-W phase would precipitate.Compared with the results of orthogonal experiment,the precipitation ofα-W phase is consistent with thermodynamic calculation results.The presence of Al not only influenced the precipitation ofα-W phase but also impacted the eutectic content and theγʹ-size,both of which showed an increase with higher Al concentrations.Excessive W and Al contents promoted the precipitation ofα-W phase,escalating the site of crack nucleation,and ultimately decreasing the plasticity.In the process of creep deformation(975℃/235 MPa),the raftedγ'phases were more continuous with increasing W contents,which increased the difficulty of dislocation climbing.As Al content increased,the density of interfacial dislocation network increased.The dislocations were entangled with each other,and the hindrance of dislocation movement was enhanced,which improved the stress rupture life.However,the precipitation of the hard and brittleα-W phase was attributed to the excessive W and Al,which increased the tendency of crack formation and significantly diminished the stress rupture life.The alloy exhibited the highest stress rupture life of 110.46 h when the W and Al contents were 15.7 wt%and 5.9 wt%,respectively.
文摘Wire⁃Electrical Discharge Machining(WEDM)process can create any complex contour in any conducting material,regardless of its strength or hardness,with higher accuracy.Present experimental investigation aims to determine suitable input process/machining parameters,e.g.pulse on time(TON),peak current(IP),wire feed rate(WF),and pulse off time(TOFF),for optimizing the process performances,namely,cutting rate,kerf width,average roughness value of the machined surface,micro hardness,and surface crack density.Since four input parameters are considered in the present investigation,and each parameter is assumed to vary at three different levels(i.e.low,medium,high),the Taguchi L9 Orthogonal Array(OA)design approach has been used for the experimental purpose to enhance the process effiency.Similarly,a simple and popular multiresponse optimization approach,namely Grey Relational Analysis(GRA),is used to optimize five performance characteristics(output responses)simultaneously.The optimum process variables obtained are:TON of 110μs,TOFF of 40μs,IP of 10 A,and WF of 6 mm/min.These optimum process variables are validated with confirmatory experiment.The relative impact of input variables is determined using the Analysis of Variance(ANOVA)technique.Finally,correlations between individual outputs with different input parameters are established.This work will be helpful for industry personnel to use this machining process in a techno⁃economic way.
基金supported in part by National Natural Science Foundation of China(NSFC)under grant 42274139in part by the R&D Department of China National Petroleum Corporation(Investigations on fundamental experiments and advanced theoretical methods in geophysical prospecting applications,2022DQ0604-03).
文摘Seismic wavefields propagate through three-dimensional(3D)space,and their precise characterization is crucial for understanding subsurface structures.Traditional 2D algorithms,due to their limitations,are insufficient to fully represent three-dimensional wavefields.The classic 3D Radon transform algorithm assumes that the wavefield's propagation characteristics are consistent in all directions,which often does not hold true in complex underground media.To address this issue,we present an improved 3D three-parameter Radon algorithm that considers the wavefield variation with azimuth and provides a more accurate wavefield description.However,introducing new parameters to describe the azimuthal varia-tion also poses computational challenges.The new Radon transform operator involves five variables and cannot be simply decomposed into small matrices for efficient computation;instead,it requires large matrix multiplication and inversion operations,significantly increasing the computational load.To overcome this challenge,we have integrated the curvature and frequency parameters,simplifying all frequency operators to the same,thereby significantly improving computation efficiency.Furthermore,existing transform algorithms neglect the lateral variation of seismic amplitudes,leading to discrepancies between the estimated multiples and those in the data.To enhance the amplitude preservation of the algorithm,we employ orthogonal polynomial fitting to capture the amplitude spatial variation in 3D seismic data.Combining these improvements,we propose a fast,amplitude-preserving,3D three-parameter Radon transform algorithm.This algorithm not only enhances computational efficiency while maintaining the original wavefield characteristics,but also improves the representation of seismic data by increasing amplitude fidelity.We validated the algorithm in multiple attenuation using both synthetic and real seismic data.The results demonstrate that the new algorithm significantly improves both accuracy and computational efficiency,providing an effective tool for analyzing seismic wavefields in complex subsurface structures.
基金financially supported by Natural Science Foundation of Anhui Provincial Department of Education(2022AH050337)State Key Laboratory of Safety and Health for Metal Mines(2022-JSKSSYS-04)the Project of National Key Research and Development Program(2022YFC3901405).
文摘Nitrogen oxides(NO_(x))and particulate matter(PM)present significant risks to both human health and environmental sustainability.The Integrated Dust Removal and Denitrification Technology(DRDt)offers a more efficient and cost-effective solution for achieving ultralow industrial flue gas emissions;however,its effectiveness is undermined by low catalyst load rates and poor stability in filter materials.This study addresses these limitations by modifying conventional PTFE filter media(PTFE-Tim)through the incorporation of sodium alginate(SA)and dopamine(DA)as modifiers,resulting in two new filter materials:PTFE-SA-MOF and PTFE-DA-MOF.By optimizing the parameters of an orthogonal experimental design,we identified the ideal preparation conditions for these composite materials.The addition of SA and DA enhanced the bonding between the catalyst(Mn-Cu-MOF)crystal particles and the PTFE fibers through mechanisms such as ion exchange,hydrogen bonding,and adhesion.Consequently,the catalyst loading rate and stability of the DRDt filters were significantly improved.Specifically,the PTFE-SA-MOF and PTFE-DA-MOF filters achieved high catalyst loading rates of 15.97% and 15.86%,these values represent improvements of 2.53 and 2.51 times,while maintaining excellent stability,with mass retention rates of 98.64% and 98.27%,respectively,over the conventional PTFE-Tim filter.
文摘Recent advancements in computational and database technologies have led to the exponential growth of large-scale medical datasets,significantly increasing data complexity and dimensionality in medical diagnostics.Efficient feature selection methods are critical for improving diagnostic accuracy,reducing computational costs,and enhancing the interpretability of predictive models.Particle Swarm Optimization(PSO),a widely used metaheuristic inspired by swarm intelligence,has shown considerable promise in feature selection tasks.However,conventional PSO often suffers from premature convergence and limited exploration capabilities,particularly in high-dimensional spaces.To overcome these limitations,this study proposes an enhanced PSO framework incorporating Orthogonal Initializa-tion and a Crossover Operator(OrPSOC).Orthogonal Initialization ensures a diverse and uniformly distributed initial particle population,substantially improving the algorithm’s exploration capability.The Crossover Operator,inspired by genetic algorithms,introduces additional diversity during the search process,effectively mitigating premature convergence and enhancing global search performance.The effectiveness of OrPSOC was rigorously evaluated on three benchmark medical datasets—Colon,Leukemia,and Prostate Tumor.Comparative analyses were conducted against traditional filter-based methods,including Fast Clustering-Based Feature Selection Technique(Fast-C),Minimum Redundancy Maximum Relevance(MinRedMaxRel),and Five-Way Joint Mutual Information(FJMI),as well as prominent metaheuristic algorithms such as standard PSO,Ant Colony Optimization(ACO),Comprehensive Learning Gravitational Search Algorithm(CLGSA),and Fuzzy-Based CLGSA(FCLGSA).Experimental results demonstrated that OrPSOC consistently outperformed these existing methods in terms of classification accuracy,computational efficiency,and result stability,achieving significant improvements even with fewer selected features.Additionally,a sensitivity analysis of the crossover parameter provided valuable insights into parameter tuning and its impact on model performance.These findings highlight the superiority and robustness of the proposed OrPSOC approach for feature selection in medical diagnostic applications and underscore its potential for broader adoption in various high-dimensional,data-driven fields.
基金support from the China Postdoctoral Science Foundation(No.2023M742735)the Postdoctoral Fellowship Program of CPSF,China(No.GZC20232029)+5 种基金the National Natural Science Foundation of China(No.52475530)the Shaanxi Postdoctoral Science Foundation,China(No.2023BSHEDZZ175)the Innovation Capability Support Program of Shaanxi Province,China(No.2021TD-23)the Key Industrial Chain Core Technology Research Project in Xi’an,China(No.23LLRH0029)the Natural Science Basic Research Program of Shaanxi,China(No.2024JC-YBQN-0490)the Fundamental Research Funds for the Central Universities,China(No.ZYTS24023).
文摘Silicon Carbide (SiC) wafers have been widely used in micro- and nano-devices due to their excellent optical and material properties. However, polishing SiC wafers has been challenging and inefficient, tending to cause significant surface crack and subsurface damage. This work proposed modifying SiC surface properties by ion implantation to improve machining efficiency, suppress surface crack, and reduce damage. High-energy ion implantation disrupted the SiC crystal lattice, reducing hardness and elastic modulus while increasing brittle-ductile transition depth, thus changing the removal mode from brittle fracture to plastic removal. Theoretical models of material removal rate and surface roughness were established for abrasive polishing of the SiC wafers. Polishing experiments were conducted on ion-implanted, modified SiC samples. The improvement mechanisms of ion implantation on surface damage, removal rate, morphology, and residual stress were investigated. The effect of ion implantation on the polished surface quality of SiC was investigated through orthogonal experiments. The results showed that ion implantation can significantly improve the average material removal rate of the SiC samples. Additionally, the ion-implanted samples had exhibited remarkable reductions in surface roughness, surface damage, and tensile residual stress.
基金Supported by the Higher Education Science Research Project(Natural Science)of Anhui Province(Grant No.2023AH050487)。
文摘In this paper,we introduce a new geometric constant R_(X)(κ)based on isosceles orthogonality.First,we explore some basic properties of this new constant and then provide several examples to estimate its exact values in certain specific Banach spaces.Next,we investigate the relationships between this new constant and other classical constants.Specifically,we establish an inequality relationship between it and the J(X)constant,as well as an identity relationship between it and theρX(t)constant.Furthermore,we characterize some geometric properties of Banach spaces by means of this new constant.Finally,by restricting the above-mentioned constant to the unit sphere,we introduce another new constant,calculate its upper and lower bounds,and present a relevant example.
基金supported by the fund from Tianjin Municipal Science and Technology Bureau(22JCYBJC01390).
文摘At present,the polymerase chain reaction(PCR)amplification-based file retrieval method is the mostcommonly used and effective means of DNA file retrieval.The number of orthogonal primers limitsthe number of files that can be accurately accessed,which in turn affects the density in a single oligo poolof digital DNA storage.In this paper,a multi-mode DNA sequence design method based on PCR file retrie-val in a single oligonucleotide pool is proposed for high-capacity DNA data storage.Firstly,by analyzingthe maximum number of orthogonal primers at each predicted primer length,it was found that the rela-tionship between primer length and the maximum available primer number does not increase linearly,and the maximum number of orthogonal primers is on the order of 10^(4).Next,this paper analyzes themaximum address space capacity of DNA sequences with different types of primer binding sites for filemapping.In the case where the capacity of the primer library is R(where R is even),the number ofaddress spaces that can be mapped by the single-primer DNA sequence design scheme proposed in thispaper is four times that of the previous one,and the two-level primer DNA sequence design scheme can reach [R/2·(R/2-1)]^(2)times.Finally,a multi-mode DNA sequence generation method is designed based onthe number of files to be stored in the oligonucleotide pool,in order to meet the requirements of the ran-dom retrieval of target files in an oligonucleotide pool with large-scale file numbers.The performance ofthe primers generated by the orthogonal primer library generator proposed in this paper is verified,andthe average Gibbs free energy of the most stable heterodimer formed between the orthogonal primersproduced is−1 kcal·(mol·L^(−1))^(−1)(1 kcal=4.184 kJ).At the same time,by selectively PCR-amplifying theDNA sequences of the two-level primer binding sites for random access,the target sequence can be accu-rately read with a minimum of 10^(3) reads,when the primer binding site sequences at different positionsare mutually different.This paper provides a pipeline for orthogonal primer library generation and multi-mode mapping schemes between files and primers,which can help achieve precise random access to filesin large-scale DNA oligo pools.
