Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,a...Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,and screen the biometric operational taxonomic units(OTUs)to assist the clinical diagnosis.Methods:We recruited children according to the“GHRS diagnostic scale”,collected their oropharyngeal swabs,and sequenced the 16 SrDNA V4 region.We described the bacterial structure with alpha-indexes,beta-distances,and relative abundances;moreover,we screened the differential genera/OTUs with Wilcoxon rank-sum test,Metagenome Seq analysis,and linear discriminant analysis effect size(LEf Se)analysis,in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value.The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.Results:The study population was composed of 10 children with GHRS and 10 healthy control children.GHRS children were more likely to overeat(gluttony,P=.033).Alpha-indexes,such as Sobs,abundancebased coverage estimator,Bootstrap,and Qstat,were significantly higher in the GHRS group,while betadistances did not exhibit any significant intergroup differences.There were 9 differently distributed nonpredominant genera between the groups in Wilcoxon rank-sum test,as well as 13 non-predominant genera in Metagenome Seq analysis and 3 non-predominant OTUs in LEfSe analysis.OTU44 and OTU196 were used to construct the receiver operating characteristic curve,and the area under curve was 0.92.Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples,while the pathway related to renin secretion was remarkably enriched in GHRS samples.Conclusion:Unique oropharyngeal microbial structure and function were identified in GHRS children.OTU44 and OTU196 were specific OTUs,which could be used as biomarkers of GHRS to assist clinical diagnosis.展开更多
Introduction: The foreign bodies of the oropharynx are mainly encountered in children. They rarely raise diagnostic problem, but remain a haunting of the CCF and ENT surgeon. Objective: We report a special case of for...Introduction: The foreign bodies of the oropharynx are mainly encountered in children. They rarely raise diagnostic problem, but remain a haunting of the CCF and ENT surgeon. Objective: We report a special case of foreign body entering the oropharynx and measuring 15 cm of long in a boy of 7 years, in order to discuss the diagnostic and therapeutic approach. Observation: A 7 year old male student has been received in emergency for accidental trauma of the oropharynx by a particular object, a pencil. The diagnosis has been essentially clinical. The exhibition of the oropharynx using the open mouth of Boyles Davis has obviously shown the foreign body penetrating the right anterior pillar crossing the parapharyngeal spaces till the right posterolateral prevertebral space. The extraction of the foreign body was done by endoscopic route under general anesthesia. As a remarkable fact, it was the gumming end that was penetrating. The postoperative course was uneventful. Conclusion: This type of foreign body of oropharynx constitutes a medical and surgical emergency. From an easy and positive diagnosis, these foreign bodies especially raise a problem of lesion diagnostic and therapeutic approach. Prevention through education and awareness of all the actors (students, children, parents) remain the pledge of their control.展开更多
Objective: To investigate the CT and MRI findings of lymphoma of oropharynx and their clinical values. Methods: CT and MRI findings of 18 cases of lymphoma of oropharynx were analyzed and compared with the operative a...Objective: To investigate the CT and MRI findings of lymphoma of oropharynx and their clinical values. Methods: CT and MRI findings of 18 cases of lymphoma of oropharynx were analyzed and compared with the operative and pathological findings. Results: 11 cases of lymphoma of the tonsil and 3 cases of lymphoma of the bases of tongue displayed regular soft tissue mass with protuberated into oropharynx. 4 cases of lymphoma of the lateral pharynx displayed irregular soft tissue mass in oropharynx. Their density and signal of the lymphoma were homogeneous and showed slight enhancement by CT and MRI. All lesions did not appear necrosis or cyst. 10 cases of cervical lymphoid metastasis were found in 18 cases of lymphoma of oropharynx. Conclusion: CT and MRI can provide the position, shape and range of lymphoma of oropharynx and metastases in lymph nodes and invasion to surrounding tissues. They had high clinical value in diagnosis and treatment of lymphoma of oropharynx.展开更多
Squamous cell carcinomas are the commonest malignancies of the head and neck. Metastases from stage III and stage IV tumors occur most commonly in the cervical lymph nodes. The incidence of distant metastases occurrin...Squamous cell carcinomas are the commonest malignancies of the head and neck. Metastases from stage III and stage IV tumors occur most commonly in the cervical lymph nodes. The incidence of distant metastases occurring from such advanced tumors is anywhere between 10% and 40%. Distant metastases occur most commonly to the lungs followed by the bone and liver. The bone metastasis occurs commonly in the axial skeleton. We report a rather unusual case of squamous cell carcinomas from the Head and Neck region in a 77-year-old male metastasizing to the ulna. This case is even more interesting because the presenting symptom was a pathological fracture of the ulna for which he had reported to the orthopedic department. The immunohistochemistry of the metastatic tumor had shown an unmistakable squamous cell carcinoma with positive cytokeratin elements within the tumor. He referred to the ENT department where he was diagnosed with T2N0M1 squamous cell carcinoma of the oropharynx. The patient was treated with internal fixation and bone cementing for the metastatic lesion, and primarily treated with chemoradiation.展开更多
基金supported by the National Natural Science Foundation of China(81373769)the Scientific Research and Postgraduate Training Co-construction Project of Beijing Municipal Education Commission(1000062520115)the Province Natural Science Foundation of Beijing(7172131)。
文摘Objective:To explore the structural and functional characteristics of microbiota in oropharynx of subhealthy children with gastrointestinal heat retention syndrome(GHRS)differentiated by traditional Chinese medicine,and screen the biometric operational taxonomic units(OTUs)to assist the clinical diagnosis.Methods:We recruited children according to the“GHRS diagnostic scale”,collected their oropharyngeal swabs,and sequenced the 16 SrDNA V4 region.We described the bacterial structure with alpha-indexes,beta-distances,and relative abundances;moreover,we screened the differential genera/OTUs with Wilcoxon rank-sum test,Metagenome Seq analysis,and linear discriminant analysis effect size(LEf Se)analysis,in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value.The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.Results:The study population was composed of 10 children with GHRS and 10 healthy control children.GHRS children were more likely to overeat(gluttony,P=.033).Alpha-indexes,such as Sobs,abundancebased coverage estimator,Bootstrap,and Qstat,were significantly higher in the GHRS group,while betadistances did not exhibit any significant intergroup differences.There were 9 differently distributed nonpredominant genera between the groups in Wilcoxon rank-sum test,as well as 13 non-predominant genera in Metagenome Seq analysis and 3 non-predominant OTUs in LEfSe analysis.OTU44 and OTU196 were used to construct the receiver operating characteristic curve,and the area under curve was 0.92.Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples,while the pathway related to renin secretion was remarkably enriched in GHRS samples.Conclusion:Unique oropharyngeal microbial structure and function were identified in GHRS children.OTU44 and OTU196 were specific OTUs,which could be used as biomarkers of GHRS to assist clinical diagnosis.
文摘Introduction: The foreign bodies of the oropharynx are mainly encountered in children. They rarely raise diagnostic problem, but remain a haunting of the CCF and ENT surgeon. Objective: We report a special case of foreign body entering the oropharynx and measuring 15 cm of long in a boy of 7 years, in order to discuss the diagnostic and therapeutic approach. Observation: A 7 year old male student has been received in emergency for accidental trauma of the oropharynx by a particular object, a pencil. The diagnosis has been essentially clinical. The exhibition of the oropharynx using the open mouth of Boyles Davis has obviously shown the foreign body penetrating the right anterior pillar crossing the parapharyngeal spaces till the right posterolateral prevertebral space. The extraction of the foreign body was done by endoscopic route under general anesthesia. As a remarkable fact, it was the gumming end that was penetrating. The postoperative course was uneventful. Conclusion: This type of foreign body of oropharynx constitutes a medical and surgical emergency. From an easy and positive diagnosis, these foreign bodies especially raise a problem of lesion diagnostic and therapeutic approach. Prevention through education and awareness of all the actors (students, children, parents) remain the pledge of their control.
文摘Objective: To investigate the CT and MRI findings of lymphoma of oropharynx and their clinical values. Methods: CT and MRI findings of 18 cases of lymphoma of oropharynx were analyzed and compared with the operative and pathological findings. Results: 11 cases of lymphoma of the tonsil and 3 cases of lymphoma of the bases of tongue displayed regular soft tissue mass with protuberated into oropharynx. 4 cases of lymphoma of the lateral pharynx displayed irregular soft tissue mass in oropharynx. Their density and signal of the lymphoma were homogeneous and showed slight enhancement by CT and MRI. All lesions did not appear necrosis or cyst. 10 cases of cervical lymphoid metastasis were found in 18 cases of lymphoma of oropharynx. Conclusion: CT and MRI can provide the position, shape and range of lymphoma of oropharynx and metastases in lymph nodes and invasion to surrounding tissues. They had high clinical value in diagnosis and treatment of lymphoma of oropharynx.
文摘Squamous cell carcinomas are the commonest malignancies of the head and neck. Metastases from stage III and stage IV tumors occur most commonly in the cervical lymph nodes. The incidence of distant metastases occurring from such advanced tumors is anywhere between 10% and 40%. Distant metastases occur most commonly to the lungs followed by the bone and liver. The bone metastasis occurs commonly in the axial skeleton. We report a rather unusual case of squamous cell carcinomas from the Head and Neck region in a 77-year-old male metastasizing to the ulna. This case is even more interesting because the presenting symptom was a pathological fracture of the ulna for which he had reported to the orthopedic department. The immunohistochemistry of the metastatic tumor had shown an unmistakable squamous cell carcinoma with positive cytokeratin elements within the tumor. He referred to the ENT department where he was diagnosed with T2N0M1 squamous cell carcinoma of the oropharynx. The patient was treated with internal fixation and bone cementing for the metastatic lesion, and primarily treated with chemoradiation.
