In the context of Western medicine,Xin(心heart),Gan(肝liver),Pi(脾spleen),Fei(肺lung),and Shen(肾kidney)are five specific organs,while in ancient Chinese medicine,they are five kinds of Zang Xiang(藏象visceral manifes...In the context of Western medicine,Xin(心heart),Gan(肝liver),Pi(脾spleen),Fei(肺lung),and Shen(肾kidney)are five specific organs,while in ancient Chinese medicine,they are five kinds of Zang Xiang(藏象visceral manifestation),often referred to as Wu Zang(五藏five zang-organs),representing five interrelated structural-functional systems.There are both differences and connections between visceral manifestations and organs,which are reflected in the theories of Wu Zang.The specific connotation of Wu Zang are determined by the specific context in which they are located.With the accumulation of practice,the Wu Zang of the human body structures and functions,as well as the relationship between the Wu Zang have been in constant development and change.The encounter of Chinese and Western medicine in the 19th century dramatically changed the connotation of Wu Zang,from emphasizing visceral manifestations and neglecting substance to an organs-oriented perspective.This article examines how the encounter of Chinese and Western medicine in China influenced Chinese medicine.By analyzing three key aspects:(1)the interpretation of Wu Zang in pre-16th century texts;(2)the evolution and dissemination of Western medicine during the Ming and Qing dynasties;(3)the shifting conceptualization of Wu Zang in late Qing medical literature—the study elucidates the transformative impact of this cross-cultural medical encounter on the theory and practice of Chinese medicine.展开更多
The high incidence of hand,foot,and mouth disease(HFMD)in children,coupled with improper management,can lead to complications,causing significant distress to both patients and their parents.This article reports on the...The high incidence of hand,foot,and mouth disease(HFMD)in children,coupled with improper management,can lead to complications,causing significant distress to both patients and their parents.This article reports on the successful treatment of a case of HFMD with dampness-heat accumulation in the spleen type using a therapeutic approach of applying herbal patches to acupoints to disperse dampness,promote heat dissipation,and unblock the fu organs.The herbs selected primarily include honeysuckle,Forsythia,and mint to relieve the exterior and promote heat dissipation;Agastache and magnolia bark to transform and dry dampness;Scutellaria,blackberry lily,and licorice to clear heat,detoxify,and benefit the throat;and magnolia bark and bitter orange to unblock the fu organs and purge heat.The flexible combination of these herbs with acupoints such as Shenque,Zhongwan,Tiantu,and Feishu fully leverages the synergistic effects of both the herbs and acupoints,resulting in a significant therapeutic effect.This approach provides valuable insights and methodologies for the prevention and treatment of HFMD in children.展开更多
BACKGROUND Traditional limitations of cold static storage(CSS)on ice at 4℃during lung transplantation have necessitated limiting cold ischemic time(CIT)to 4-6 hours.Ex vivo lung perfusion(EVLP)can extend this preserv...BACKGROUND Traditional limitations of cold static storage(CSS)on ice at 4℃during lung transplantation have necessitated limiting cold ischemic time(CIT)to 4-6 hours.Ex vivo lung perfusion(EVLP)can extend this preservation time through the suspension of CIT and normothermic perfusion.As we continue to further expand the donor pool in all aspects of lung transplantation,teams are frequently traveling further distances to procure organs.AIM To determine the effect of CSS or EVLP on donors with extended travel distance[>750 nautical miles(NM)]to recipient.METHODS Lung transplants,whose donor traveled greater than 750 NM,were identified from the United Network for Organ Sharing Database.Recipients were stratified into either:CSS or EVLP,based on preservation method.Groups were assessed with comparative statistics and survival was assessed by Kaplan-Meier methods.A 3:1 propensity match was then created,and same analysis was repeated.RESULTS Prior to matching,those in the EVLP group had significantly increased postoperative morbidity to include dialysis,ventilator use,acute rejection,and treated rejection in the first year(P<0.05 for all).However,there were no significant differences in midterm survival(P=0.18).Following matching,those in the EVLP group again had significantly increased post-operative morbidity to include dialysis,extracorporeal membrane oxygenation use,ventilator use,and treated rejection in the first year(P<0.05 for all).As before,there were no significant differences in midterm survival following matching(P=0.08).CONCLUSION While there was no significant difference in survival,EVLP patients had increased peri-operative morbidity.With the advent of changes in CSS with 10℃storage further analysis is necessary to evaluate the best methods for utilizing organs from increased distances.展开更多
Particulate pollution is a global risk factor that seriously threatens human health.Fine particles(FPs)and ultrafine particles(UFPs)have small particle diameters and large specific surface areas,which can easily adsor...Particulate pollution is a global risk factor that seriously threatens human health.Fine particles(FPs)and ultrafine particles(UFPs)have small particle diameters and large specific surface areas,which can easily adsorb metals,microorganisms and other pollutants.FPs and UFPs can enter the human body in multiple ways and can be easily and quickly absorbed by the cells,tissues and organs.In the body,the particles can induce oxidative stress,inflammatory response and apoptosis,furthermore causing great adverse effects.Epidemiological studies mainly take the population as the research object to study the distribution of diseases and health conditions in a specific population and to focus on the identification of influencing factors.However,the mechanism by which a substance harms the health of organisms is mainly demonstrated through toxicological studies.Combining epidemiological studies with toxicological studies will provide a more systematic and comprehensive understanding of the impact of PM on the health of organisms.In this review,the sources,compositions,and morphologies of FPs and UFPs are briefly introduced in the first part.The effects and action mechanisms of exposure to FPs and UFPs on the heart,lungs,brain,liver,spleen,kidneys,pancreas,gastrointestinal tract,joints and reproductive system are systematically summarized.In addition,challenges are further pointed out at the end of the paper.This work provides useful theoretical guidance and a strong experimental foundation for investigating and preventing the adverse effects of FPs and UFPs on human health.展开更多
Global climate change can affect tree growth and carbon sink function by influencing plant carbohydrate synthesis and utilization,while elevation can be used as an ideal setting under natural conditions to simulate cl...Global climate change can affect tree growth and carbon sink function by influencing plant carbohydrate synthesis and utilization,while elevation can be used as an ideal setting under natural conditions to simulate climate change effects.The effect of elevation on tree growth may depend on organ type.However,the allocation patterns of nonstructural and structural carbohydrates(NSCs and SCs,respectively)in different tree organs and their response to elevation remain unclear.We selected four dominant tree species,Schima superba,Castanopsis eyrei,Castanopsis fargesii and Michelia maudiae,along an elevation gradient from 609 to 1,207 m in subtropical evergreen broad-leaved forests and analyzed leaf,trunk,and fine root NSCs,carbon(C),nitrogen(N)and phosphorus(P)concentrations and the relative abundance of SCs.Leaf NSCs increased initially and then decreased,and trunk NSCs increased with increasing elevation.However,root NSCs decreased with increasing elevation.The relative abundance of SCs in leaves and trunks decreased,while the relative abundance of root SCs increased with increasing elevation.No significant correlations between SCs and NSCs in leaves were detected,while there were negative correlations between SCs and NSCs in trunks,roots,and all organs.Hierarchical partitioning analysis indicated that plant C/N and C/P were the main predictors of changes in SCs and NSCs.Our results suggest that tree organs have divergent responses to elevation and that increasing elevation will inhibit the aboveground part growth and enhance the root growth of trees.A tradeoff between the C distribution used for growth and storage was confirmed along the elevation gradient,which is mainly manifested in the"sink"organs of NSCs.Our results provide insight into tree growth in the context of global climate change scenarios in subtropical forest ecosystems.展开更多
Hollow tubular tissues and organs of our body have various functions: gastrointestinal (esophagus), respiratory (trachea), and vascular (veins, arteries). A panel of pathologies is associated with each of these tissue...Hollow tubular tissues and organs of our body have various functions: gastrointestinal (esophagus), respiratory (trachea), and vascular (veins, arteries). A panel of pathologies is associated with each of these tissues and therapeutic interventions, surgery or replacement may be necessary. A precise knowledge of the mechanical properties of these tissues is thus required in order to understand their functioning in native conditions, to be able to elaborate some prostheses, or to design appropriate surgical training tools. These tissues may undergo expansions or contractions (peristalsis) and are exposed to internal pressures. The wall of tubular organs is organized in different layers, and each layer consists of various cell types and extra-cellular matrix, depending on the physiological functions that the organ has to fulfil. This yields anisotropic and compliant structures. In inflation experiments, the linear elasticity approach is acceptable as long as the organ’s inflation remains moderate. In this paper, elasticity laws are revisited and supplemented in order to show that, coupled with modern experimental characterization tools, they provide useful information (compliances, directional Young moduli, Poisson ratios) for the design of artificial tubular organs. The importance of a precise determination of the wall thickness and its evolution during inflation is pointed out.展开更多
Spinal cord injury is a serious disease of the central nervous system involving irreversible nerve injury and various organ system injuries.At present,no effective clinical treatment exists.As one of the artificial hi...Spinal cord injury is a serious disease of the central nervous system involving irreversible nerve injury and various organ system injuries.At present,no effective clinical treatment exists.As one of the artificial hibernation techniques,mild hypothermia has preliminarily confirmed its clinical effect on spinal cord injury.However,its technical defects and barriers,along with serious clinical side effects,restrict its clinical application for spinal cord injury.Artificial hibernation is a futureoriented disruptive technology for human life support.It involves endogenous hibernation inducers and hibernation-related central neuromodulation that activate particular neurons,reduce the central constant temperature setting point,disrupt the normal constant body temperature,make the body adapt"to the external cold environment,and reduce the physiological resistance to cold stimulation.Thus,studying the artificial hibernation mechanism may help develop new treatment strategies more suitable for clinical use than the cooling method of mild hypothermia technology.This review introduces artificial hibernation technologies,including mild hypothermia technology,hibernation inducers,and hibernation-related central neuromodulation technology.It summarizes the relevant research on hypothermia and hibernation for organ and nerve protection.These studies show that artificial hibernation technologies have therapeutic significance on nerve injury after spinal co rd injury through inflammatory inhibition,immunosuppression,oxidative defense,and possible central protection.It also promotes the repair and protection of res pirato ry and digestive,cardiovascular,locomoto r,urinary,and endocrine systems.This review provides new insights for the clinical treatment of nerve and multiple organ protection after spinal cord injury thanks to artificial hibernation.At present,artificial hibernation technology is not mature,and research fa ces various challenges.Neve rtheless,the effort is wo rthwhile for the future development of medicine.展开更多
Maize plant architecture influences planting density and,in turn,grain yield.Most of the plant architecture-related traits can be described as organ size.We describe a miniature maize mutant,Tiny plant 4(Tip4),which e...Maize plant architecture influences planting density and,in turn,grain yield.Most of the plant architecture-related traits can be described as organ size.We describe a miniature maize mutant,Tiny plant 4(Tip4),which exhibits reduced size of multiple organs and exhibits a semi-dominant monofactorial inheritance characteristic.Positional cloning confirmed that a 4-bp deletion in the NAC TF with transmembrane motif 1-Like(NTL)gene ZmNTL2,denoted as ZmNTL2^(Δ),confers the Tip4 mutation.qRT-PCR showed that ZmNTL2 was expressed in all tested tissues.ZmNTL2 functions as a transcriptional activator and is located in both the nucleus and biomembranes.The mutation does not affect the mRNA abundance of ZmNTL2 locus,but it does result in the loss of transmembrane domain and confines the ZmNTL2^(Δ)protein to the nucleus.Knocking out ZmNTL2 has no effect on maize organ size development,indicating that the 4-bp deletion might be a gain-of-function mutation in organ size regulation.Combining transcriptome sequencing with cytokinin and auxin content determination suggests that the decreased organ size may be possibly mediated by changes in hormone homeostasis.展开更多
The floral organ morphology,pollen morphology,quantity,and viability of Rumex,Rumex hanus F1,Rumex hanus 1,and Rumex hanus 2 were compared and analyzed.The results showed that the longitudinal diameter of petals was n...The floral organ morphology,pollen morphology,quantity,and viability of Rumex,Rumex hanus F1,Rumex hanus 1,and Rumex hanus 2 were compared and analyzed.The results showed that the longitudinal diameter of petals was not significantly different among different varieties of sorrel,and the transverse diameter and corolla diameter showed differences to varying degrees.They all had six stamens and three stigmas.The pollen shape of the four varieties was prolate,and the polar view was trilobated and round.They all had three germinal furrows,and the outer wall of the pollen was decorated with small cavities and spiny patterns,with warty protrusions.The equatorial axis length of pollen of different varieties of sorrel was significantly different(P<0.05).There were no significant differences in pollen amount among different varieties.There were six anthers in a single flower,featuring anther dehiscence.The pollen viability of Rumex hanus 1 was significantly lower than that of the other three varieties(P<0.05).Therefore,through the analysis of the floral organs and morphological characteristics of anthers of sorrel,the varieties of sorrel can be effectively distinguished and identified.展开更多
In this paper, ontogeny of immune-related organs during theearly development of carp was studied by histochemical technique-lcineBlue staining under acid conditions and PAS reaction(AB-PAS staining). Thekidney appeare...In this paper, ontogeny of immune-related organs during theearly development of carp was studied by histochemical technique-lcineBlue staining under acid conditions and PAS reaction(AB-PAS staining). Thekidney appeared one day before hatching, spleen, liver and pancreas emergedon the same day of hatching, and thymus was not found until the third day afterhatching. Ontogeny of these immune related organs of the carp in thisresearch is earlier than that reported by Botham.展开更多
This editorial describes the indications and technical aspects of the simultaneous retrieval of thoracic and abdominal organs in Maastricht III donors as well as the preservation of such organs until their implantation.
AIM: To observe the therapeutic effects of dexamethasone on rats with severe acute pancreatitis (SAP) and investigate the influences of dexamethasone on the inflammatory mediators and NF-κB expression in multiple org...AIM: To observe the therapeutic effects of dexamethasone on rats with severe acute pancreatitis (SAP) and investigate the influences of dexamethasone on the inflammatory mediators and NF-κB expression in multiple organs of SAP rats as well as the mechanisms involved. METHODS: Ninety Sprague-Dawley (SD) rats with SAP were randomly divided into the model group (n = 45) and dexamethasone treatment group (n = 45), and another 45 rats were selected for the sham operation group. All groups were randomly subdivided into the 3 h, 6 h and 12 h groups, each group containing 15 rats. The survival of all groups and pathological changes of multiple organs (liver, kidney and lung) were observed at different time points after the operation. The pathologicalscore of multiple organs was carried out, followed by the determination of amylase, endotoxin and TNF-α contents in blood. The tissue microarray was used to detect the expression levels of NF-κB p65 protein in multiple organs. RESULTS: There was no marked difference between the model group and treatment group in the survival rate. The amylase content of the treatment group was significantly lower compared to the model group at 12 h (P < 0.01, 7791.00 vs 9195.00). Moreover, the endotoxin and TNF-α levels of the treatment group were significantly lower than that of the model group at 6 h and 12 h (P < 0.01, 0.040 vs 0.055, 0.042 vs 0.059 and P < 0.05, 58.30 vs 77.54, 38.70 vs 67.30, respectively). Regarding the changes in liver NF-κB expression, the model group significantly exceeded the sham operation group at 3 h (P < 0.01, 1.00 vs 0.00), and the treatment group significantly exceeded the sham operation group at 12 h (P < 0.01, 1.00 vs 0.00), whereas no marked difference was observed between the model group and treatment group at all time points. The kidney NF-κB expression level in the treatment group significantly exceeded the model group (P < 0.05, 2.00 vs 0.00) and the sham operation group (P < 0.01, 2.00 vs 0.00) at 12 h. No NF-κB expression in the lung was found in any group. CONCLUSION: Dexamethasone can lower the amylase, endotoxin and TNF-α levels as well as mortality of SAP rats. NF-κB plays an important role in multiple organ injury. Further studies should be conducted to determine whether dexamethasone can ameliorate the pathological changes of multiple organs by reducing the NF-κB expression in the liver and kidney. The advantages of tissue microarrays in pancreatitis pathological examination include time- and energy- saving, and are highly efficient and representative. The restriction of tissue microarrays on the representation of tissues to various extents due to small diameter may lead to the deviation of analysis.展开更多
Objective:To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis (SAP). Methods:The rats were divided into sha...Objective:To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis (SAP). Methods:The rats were divided into sham-operated, model control, dexamethasone treated, and Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, Bcl-2-associated X protein (Bax) and nuclear factor-κB (NF-κB) protein expression levels in multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (BUN), and creatinine (CREA) were observed. Results:The mortality rate of the dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of Bax and NF-κB proteins, and apoptotic indexes of multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, BUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the dexamethasone treated group was significantly lower than that in the Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and BUN (at 6 h) of the Salvia miltiorrhiza treated group were significantly lower than those in the dexamethasone treated group (P<0.05). Conclusions:Both dexamethasone and Salvia miltiorrhiza can reduce the inflammatory reaction, regulate apoptosis, and thus protect multiple organs of rats with SAP.展开更多
Under the environment of an artificial climate chamber, supercooling point （SCP） and freezing point （FP） in flower and young fruit at different development stages and freezing injuries of floral organs were studie...Under the environment of an artificial climate chamber, supercooling point （SCP） and freezing point （FP） in flower and young fruit at different development stages and freezing injuries of floral organs were studied. The apricot cultivars tested were Kety, Golden Sun and Honghebao. With the development of flower buds, SCP and FP increased, which indicated that their cold resistance decreased. SCP and FP varied with different floral organs. For different apricot cultivars, it was found that, the lower SCP or FP in floral organs was, the more resistant capacity the cultivar had, and the larger the temperature interval from SCP to FP was. SCP was not a constant value, but a range. Frequency distribution of SCP in petals was more dispersing than that in stamens and pistils. Floral organs could maintain a supercooling state to avoid ice formation, but they were sensitive to freezing. Once floral organs froze, thev turned brown after thawing.展开更多
BACKGROUND:Severe acute pancreatitis (SAP) is characterized by fatal pathogenic conditions and a high mortality.It is important to study SAP complicated with multiple organ injury.In this study we compared the protect...BACKGROUND:Severe acute pancreatitis (SAP) is characterized by fatal pathogenic conditions and a high mortality.It is important to study SAP complicated with multiple organ injury.In this study we compared the protective effects of three traditional Chinese medicines (Ligustrazine,Kakonein and Panax Notoginsenoside) on the small intestine and immune organs (thymus,spleen and lymph nodes) of rats with SAP and explored their mechanism of action.METHODS:One hundred forty-four rats with SAP were randomly divided into model control,Ligustrazine-treated,Kakonein-treated,and Panax Notoginsenoside-treated groups (n=36 per group).Another 36 normal rats comprised the sham-operated group.According to the different time points after operation,the experimental rats in each group were subdivided into 3-,6-and 12-hour subgroups (n=12).At various time points after operation,the mortality rate of rats and pathological changes in the small intestine and immune organs were recorded and the serum amylase levels were measured.RESULTS:Compared to the model control groups,the mortality rates in all treated groups declined and the pathological changes in the small intestine and immune tissues were relieved to different degrees.The serum amylase levels in the three treated groups were significantly lower than those in the model control group at 12 hours.The pathological severity scores for the small intestinal mucosa,thymus and spleen (at 3 and 12 hours) in the Ligustrazine-treated group,for the thymus (at 3 and 12 hours) and spleen (at 3 and 6 hours) in the Kakonein-treated group,and for the thymus (at 3 hours)and spleen (at 3 hours) in the Panax Notoginsenoside-treated group were significantly lower than those in the model control group.The pathological severity scores of the small intestinal mucosa (at 6 and 12 hours) and thymus (at 6 hours) in the Ligustrazine-treated group were significantly lower than those in the Kakonein-and Panax Notoginsenoside-treated groups.CONCLUSIONS:All the three traditional Chinese drugs significantly alleviated the pathological changes in the small intestine and immune organs of SAP rats.Ligustrazine was the most effective one among them.展开更多
[Objective] This study was conducted to investigate the morphological and physiological differences between the flowers opening spring and late autumn of Hosui Asian pear（Pyrus pyrifolia Nakai）. [Method] Pear flower...[Objective] This study was conducted to investigate the morphological and physiological differences between the flowers opening spring and late autumn of Hosui Asian pear（Pyrus pyrifolia Nakai）. [Method] Pear flowers were collected from normal bloom（NB） and returning bloom periods during late autumn in 2011-2013,respectively. The morphological and physiological indices including pollen number,germination rate, petal length, soluble protein content, soluble sugar content, amino acid content, pollen tube growth, fruiting characteristics of NB and RB flowers were detected and compared. [Result] The filament length and petal area of RB flowers were significantly smaller than those of NB flowers. The contents of soluble proteins, soluble sugars and amino acids of RB pollens were significantly smaller than those of NB flowers. In addition, the abortion rate of RB flowers was higher than that of NB flowers. [Conclusion] RB flowers had complete floral organs and were capable of pollination, but they were different from NB flowers in some morphological and physiological indices.展开更多
Aim: To observe the cytotoxic effect of the organophosphate insecticide malathion in the reproductive tissues of the earthworms, Eisenia foetida. Methods: Worms were nourished in soil treated with malathion at single ...Aim: To observe the cytotoxic effect of the organophosphate insecticide malathion in the reproductive tissues of the earthworms, Eisenia foetida. Methods: Worms were nourished in soil treated with malathion at single sub-lethal doses of 0, 80, 150, 300 and 600 mg-kg^(-1) soil. (LD_(50) = 880 mg kg^(-1) soil) and evaluated on days 1, 5, 15 and 30 after exposure. The body weights were recorded and male reproductive organs evaluated. Results: Malathion-treated animals showed a significant reduction in body weight in a dose-dependent manner. Malathion treatment modified the disposition of spermatozoa in the basal epithelium of the spermatheca. The Br-deoxyuridine test showed a significant rise in cells in phase S on days 5 and 15. Also, a higher percentage of spermatogonia with fragmented DNA were observed by means of the TdT-mediated dUTP nick-end labeling (TUNEL) technique in the spermatheca of treated animals. Conclusion: Treatment with malathion decreased the body weight and the spermatic viability in spermatheca, altering the cell proliferation and modifying the DNA structure of spermatogonia.展开更多
Based on the experiment of measuring panicles and leaves, air temperature, and humidity above the canopy of rice cultivars after heading in 2005 and 2006, we investigated the temperature difference （TD） between the ...Based on the experiment of measuring panicles and leaves, air temperature, and humidity above the canopy of rice cultivars after heading in 2005 and 2006, we investigated the temperature difference （TD） between the air and organs of rice plant and its relationship with spikelet fertility. The results showed that TDs between the air and organs of rice varied with air temperature, air humidity, and plant type. For similar air humidity, TDs were lower at the air temperature of 28.5℃ than at higher temperature of 35.5℃, whereas for the same air temperature, the TDs decreased as the air humidity increased. TDs were also affected by plant type of the cultivars. Erect panicle cultivars showed higher TDs than those with droopy panicles under similar climatic conditions, and cultivars with panicles above flag leaf （PAFL） had higher TDs than those with panicles below the flag leaf （PBFL）. Cultivars grown in a location with lower air humidity and higher temperature, such as Taoyuan, China, had higher spikelet fertility than those in higher humidity under the similar air temperature during the grain filling stage. This is partially attributed to the larger TDs under the lower humidity. Rowspacing and the ratio of basal-tillering to panicle-spikelet fertilizer showed a significant influence on TD and subsequently on spikelet fertility, suggesting the possibility of increasing spikelet fertility by agronomic management.展开更多
AIM： To identify and understand the regular distribution pattern for Salmonella enteritidis （S. enteritidis） in the internal organs of mice after an oral challenge over a 3 wk period. METHODS： Assays based on the ...AIM： To identify and understand the regular distribution pattern for Salmonella enteritidis （S. enteritidis） in the internal organs of mice after an oral challenge over a 3 wk period. METHODS： Assays based on the serovar-specific DNA sequence of S. enteritidis from GenBank, and a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction （FQ-PCR） were developed for the detection of S. enteritidis. We used this assay to detect genomic DNA of S. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from mice after oral challenge at different time points respectively.RESULTS： The results showed that the spleen was positive at 12 h post inoculation （PI）, and the blood was at 14 h PI. The organism was detected in the liver and heart at 16 h PI, the pancreas was positive at 20 h PI, and the final organs to show positive results were the kidney and gallbladder at 22 h PI. The copy number of S. enteritidis DNA in each tissue reached a peak at 24-36 h PI, with the liver and spleen containing high concentrations of S. enteritidis, whereas the blood, heart, kidney, pancreas, and gallbladder had low concentrations. S. enteritidis populations began to decrease and were not detectable at 3 d PI, but were still present up to 12 d PI in the gallbladder, 2 wk for the liver, and 3 wk for the spleen without causing apparent symptoms.CONCLUSION： The results provided significant data for understanding the life cycle of S. enteritidis in the internal organs, and showed that the liver and spleen may be the primary sites for setting itself up as a commensa over a long time after oral challenge. Interestingly, it may be the first time reported that the gallbladder is a site of carriage for S. enteritidis over a 12 d period. This study will help to understand the mechanisms of action of S. enteriCdis infection in vivo.展开更多
OBJECTIVE: To investigate the relationship between symptom patterns of cold coagulation and blood stasis(CCBS) and microcirculation disturbance. In addition, we determined the efficacy of modified Wenjing decoction(WJ...OBJECTIVE: To investigate the relationship between symptom patterns of cold coagulation and blood stasis(CCBS) and microcirculation disturbance. In addition, we determined the efficacy of modified Wenjing decoction(WJD) for the treatment of CCBS.METHODS: CCBS was induced in rats with an icewater bath treatment. The ovarian function, microvascular and circulatory status of reproductive organs, and function of local microvascular endothelial cells(VECs) and vascular smooth muscle cells(VSMCs) were evaluated.RESULTS: Ovarian dysfunction was observed in the rats with CCBS. It was characterized by the presence of an estrous cycle disorder and a decrease in reproductive hormone levels. Microvascular circulation disorders were associated with an imbalance in vasoconstriction, relaxation substances, nitric oxide, abnormal blood flow in whole blood, and decreased blood flow in the auricle and uterus. VECs were damaged, and VSMCs contracted and proliferated in ovarian and uterine tissues.CONCLUSION: Our findings suggest that the dysfunctional reproductive organs observed in gynecological CCBS may be closely related to the microcirculation disturbance of local tissues, microvascular contraction, and vascular remodeling. Modified WJD can be used to treat CCBS by improving microcirculation in reproductive organs.展开更多
基金financed by the grant from The MUST Faculty Research Grants(FRG-25-031-FC)and Fundação Macao.
文摘In the context of Western medicine,Xin(心heart),Gan(肝liver),Pi(脾spleen),Fei(肺lung),and Shen(肾kidney)are five specific organs,while in ancient Chinese medicine,they are five kinds of Zang Xiang(藏象visceral manifestation),often referred to as Wu Zang(五藏five zang-organs),representing five interrelated structural-functional systems.There are both differences and connections between visceral manifestations and organs,which are reflected in the theories of Wu Zang.The specific connotation of Wu Zang are determined by the specific context in which they are located.With the accumulation of practice,the Wu Zang of the human body structures and functions,as well as the relationship between the Wu Zang have been in constant development and change.The encounter of Chinese and Western medicine in the 19th century dramatically changed the connotation of Wu Zang,from emphasizing visceral manifestations and neglecting substance to an organs-oriented perspective.This article examines how the encounter of Chinese and Western medicine in China influenced Chinese medicine.By analyzing three key aspects:(1)the interpretation of Wu Zang in pre-16th century texts;(2)the evolution and dissemination of Western medicine during the Ming and Qing dynasties;(3)the shifting conceptualization of Wu Zang in late Qing medical literature—the study elucidates the transformative impact of this cross-cultural medical encounter on the theory and practice of Chinese medicine.
文摘The high incidence of hand,foot,and mouth disease(HFMD)in children,coupled with improper management,can lead to complications,causing significant distress to both patients and their parents.This article reports on the successful treatment of a case of HFMD with dampness-heat accumulation in the spleen type using a therapeutic approach of applying herbal patches to acupoints to disperse dampness,promote heat dissipation,and unblock the fu organs.The herbs selected primarily include honeysuckle,Forsythia,and mint to relieve the exterior and promote heat dissipation;Agastache and magnolia bark to transform and dry dampness;Scutellaria,blackberry lily,and licorice to clear heat,detoxify,and benefit the throat;and magnolia bark and bitter orange to unblock the fu organs and purge heat.The flexible combination of these herbs with acupoints such as Shenque,Zhongwan,Tiantu,and Feishu fully leverages the synergistic effects of both the herbs and acupoints,resulting in a significant therapeutic effect.This approach provides valuable insights and methodologies for the prevention and treatment of HFMD in children.
基金Supported by The Jewel and Frank Benson Family Endowmentand The Jewel and Frank Benson Research Professorship.
文摘BACKGROUND Traditional limitations of cold static storage(CSS)on ice at 4℃during lung transplantation have necessitated limiting cold ischemic time(CIT)to 4-6 hours.Ex vivo lung perfusion(EVLP)can extend this preservation time through the suspension of CIT and normothermic perfusion.As we continue to further expand the donor pool in all aspects of lung transplantation,teams are frequently traveling further distances to procure organs.AIM To determine the effect of CSS or EVLP on donors with extended travel distance[>750 nautical miles(NM)]to recipient.METHODS Lung transplants,whose donor traveled greater than 750 NM,were identified from the United Network for Organ Sharing Database.Recipients were stratified into either:CSS or EVLP,based on preservation method.Groups were assessed with comparative statistics and survival was assessed by Kaplan-Meier methods.A 3:1 propensity match was then created,and same analysis was repeated.RESULTS Prior to matching,those in the EVLP group had significantly increased postoperative morbidity to include dialysis,ventilator use,acute rejection,and treated rejection in the first year(P<0.05 for all).However,there were no significant differences in midterm survival(P=0.18).Following matching,those in the EVLP group again had significantly increased post-operative morbidity to include dialysis,extracorporeal membrane oxygenation use,ventilator use,and treated rejection in the first year(P<0.05 for all).As before,there were no significant differences in midterm survival following matching(P=0.08).CONCLUSION While there was no significant difference in survival,EVLP patients had increased peri-operative morbidity.With the advent of changes in CSS with 10℃storage further analysis is necessary to evaluate the best methods for utilizing organs from increased distances.
基金financial support received from the National Natural Science Foundation of China (No.82173486)the Natural Sciences Foundation of Tianjin City of China (No.12JCYBJC19100)+1 种基金the Scientific Research Program of Tianjin Education Commission (No.2019KJ167)The Program of Tianjin Key Laboratory of Oral and Maxillofacial Function Reconstruction (No.2021KLMS10)。
文摘Particulate pollution is a global risk factor that seriously threatens human health.Fine particles(FPs)and ultrafine particles(UFPs)have small particle diameters and large specific surface areas,which can easily adsorb metals,microorganisms and other pollutants.FPs and UFPs can enter the human body in multiple ways and can be easily and quickly absorbed by the cells,tissues and organs.In the body,the particles can induce oxidative stress,inflammatory response and apoptosis,furthermore causing great adverse effects.Epidemiological studies mainly take the population as the research object to study the distribution of diseases and health conditions in a specific population and to focus on the identification of influencing factors.However,the mechanism by which a substance harms the health of organisms is mainly demonstrated through toxicological studies.Combining epidemiological studies with toxicological studies will provide a more systematic and comprehensive understanding of the impact of PM on the health of organisms.In this review,the sources,compositions,and morphologies of FPs and UFPs are briefly introduced in the first part.The effects and action mechanisms of exposure to FPs and UFPs on the heart,lungs,brain,liver,spleen,kidneys,pancreas,gastrointestinal tract,joints and reproductive system are systematically summarized.In addition,challenges are further pointed out at the end of the paper.This work provides useful theoretical guidance and a strong experimental foundation for investigating and preventing the adverse effects of FPs and UFPs on human health.
基金the National Natural Science Foundation of China(32260379&32371852)the Jiangxi Provincial Natural Science Foundation(20224ACB215005)
文摘Global climate change can affect tree growth and carbon sink function by influencing plant carbohydrate synthesis and utilization,while elevation can be used as an ideal setting under natural conditions to simulate climate change effects.The effect of elevation on tree growth may depend on organ type.However,the allocation patterns of nonstructural and structural carbohydrates(NSCs and SCs,respectively)in different tree organs and their response to elevation remain unclear.We selected four dominant tree species,Schima superba,Castanopsis eyrei,Castanopsis fargesii and Michelia maudiae,along an elevation gradient from 609 to 1,207 m in subtropical evergreen broad-leaved forests and analyzed leaf,trunk,and fine root NSCs,carbon(C),nitrogen(N)and phosphorus(P)concentrations and the relative abundance of SCs.Leaf NSCs increased initially and then decreased,and trunk NSCs increased with increasing elevation.However,root NSCs decreased with increasing elevation.The relative abundance of SCs in leaves and trunks decreased,while the relative abundance of root SCs increased with increasing elevation.No significant correlations between SCs and NSCs in leaves were detected,while there were negative correlations between SCs and NSCs in trunks,roots,and all organs.Hierarchical partitioning analysis indicated that plant C/N and C/P were the main predictors of changes in SCs and NSCs.Our results suggest that tree organs have divergent responses to elevation and that increasing elevation will inhibit the aboveground part growth and enhance the root growth of trees.A tradeoff between the C distribution used for growth and storage was confirmed along the elevation gradient,which is mainly manifested in the"sink"organs of NSCs.Our results provide insight into tree growth in the context of global climate change scenarios in subtropical forest ecosystems.
文摘Hollow tubular tissues and organs of our body have various functions: gastrointestinal (esophagus), respiratory (trachea), and vascular (veins, arteries). A panel of pathologies is associated with each of these tissues and therapeutic interventions, surgery or replacement may be necessary. A precise knowledge of the mechanical properties of these tissues is thus required in order to understand their functioning in native conditions, to be able to elaborate some prostheses, or to design appropriate surgical training tools. These tissues may undergo expansions or contractions (peristalsis) and are exposed to internal pressures. The wall of tubular organs is organized in different layers, and each layer consists of various cell types and extra-cellular matrix, depending on the physiological functions that the organ has to fulfil. This yields anisotropic and compliant structures. In inflation experiments, the linear elasticity approach is acceptable as long as the organ’s inflation remains moderate. In this paper, elasticity laws are revisited and supplemented in order to show that, coupled with modern experimental characterization tools, they provide useful information (compliances, directional Young moduli, Poisson ratios) for the design of artificial tubular organs. The importance of a precise determination of the wall thickness and its evolution during inflation is pointed out.
基金supported by the Key Projects of the National Natural Science Foundation of China,No.11932013(to XC)Key Military Logistics Research Projects,No.B WJ21J002(to XC)+4 种基金the Key projects of the Special Zone for National Defence Innovation,No.21-163-12-ZT006002-13(to XC)the National Nature Science Foundation of China No.82272255(to XC)the National Defense Science and Technology Outstanding Youth Science Fund Program,No.2021-JCIQ-ZQ-035(to XC)the Scientific Research Innovation Team Project of Armed Police Characteristic Medical Center,No.KYCXTD0104(to ZL)the National Natural Science Foundation of China Youth Fund,No.82004467(to BC)。
文摘Spinal cord injury is a serious disease of the central nervous system involving irreversible nerve injury and various organ system injuries.At present,no effective clinical treatment exists.As one of the artificial hibernation techniques,mild hypothermia has preliminarily confirmed its clinical effect on spinal cord injury.However,its technical defects and barriers,along with serious clinical side effects,restrict its clinical application for spinal cord injury.Artificial hibernation is a futureoriented disruptive technology for human life support.It involves endogenous hibernation inducers and hibernation-related central neuromodulation that activate particular neurons,reduce the central constant temperature setting point,disrupt the normal constant body temperature,make the body adapt"to the external cold environment,and reduce the physiological resistance to cold stimulation.Thus,studying the artificial hibernation mechanism may help develop new treatment strategies more suitable for clinical use than the cooling method of mild hypothermia technology.This review introduces artificial hibernation technologies,including mild hypothermia technology,hibernation inducers,and hibernation-related central neuromodulation technology.It summarizes the relevant research on hypothermia and hibernation for organ and nerve protection.These studies show that artificial hibernation technologies have therapeutic significance on nerve injury after spinal co rd injury through inflammatory inhibition,immunosuppression,oxidative defense,and possible central protection.It also promotes the repair and protection of res pirato ry and digestive,cardiovascular,locomoto r,urinary,and endocrine systems.This review provides new insights for the clinical treatment of nerve and multiple organ protection after spinal cord injury thanks to artificial hibernation.At present,artificial hibernation technology is not mature,and research fa ces various challenges.Neve rtheless,the effort is wo rthwhile for the future development of medicine.
基金supported by The National Key Research and Development Program of China(2022YFD1200704--3)Crop Varietal Improvement and Insect Pests Control by Nuclear Radiation,Platform for Mutation Breeding by Radiation of Sichuan(2021YFYZ0011)+1 种基金Natural Science Foundation of Sichuan Province(2022NSFSC1635)Applied Basic Research Programs of Sichuan Provincial Science and Technology Department(2020YJ0249)。
文摘Maize plant architecture influences planting density and,in turn,grain yield.Most of the plant architecture-related traits can be described as organ size.We describe a miniature maize mutant,Tiny plant 4(Tip4),which exhibits reduced size of multiple organs and exhibits a semi-dominant monofactorial inheritance characteristic.Positional cloning confirmed that a 4-bp deletion in the NAC TF with transmembrane motif 1-Like(NTL)gene ZmNTL2,denoted as ZmNTL2^(Δ),confers the Tip4 mutation.qRT-PCR showed that ZmNTL2 was expressed in all tested tissues.ZmNTL2 functions as a transcriptional activator and is located in both the nucleus and biomembranes.The mutation does not affect the mRNA abundance of ZmNTL2 locus,but it does result in the loss of transmembrane domain and confines the ZmNTL2^(Δ)protein to the nucleus.Knocking out ZmNTL2 has no effect on maize organ size development,indicating that the 4-bp deletion might be a gain-of-function mutation in organ size regulation.Combining transcriptome sequencing with cytokinin and auxin content determination suggests that the decreased organ size may be possibly mediated by changes in hormone homeostasis.
基金Supported by National Key Research and Development Plan Project of China(2023YFD1301200)。
文摘The floral organ morphology,pollen morphology,quantity,and viability of Rumex,Rumex hanus F1,Rumex hanus 1,and Rumex hanus 2 were compared and analyzed.The results showed that the longitudinal diameter of petals was not significantly different among different varieties of sorrel,and the transverse diameter and corolla diameter showed differences to varying degrees.They all had six stamens and three stigmas.The pollen shape of the four varieties was prolate,and the polar view was trilobated and round.They all had three germinal furrows,and the outer wall of the pollen was decorated with small cavities and spiny patterns,with warty protrusions.The equatorial axis length of pollen of different varieties of sorrel was significantly different(P<0.05).There were no significant differences in pollen amount among different varieties.There were six anthers in a single flower,featuring anther dehiscence.The pollen viability of Rumex hanus 1 was significantly lower than that of the other three varieties(P<0.05).Therefore,through the analysis of the floral organs and morphological characteristics of anthers of sorrel,the varieties of sorrel can be effectively distinguished and identified.
文摘In this paper, ontogeny of immune-related organs during theearly development of carp was studied by histochemical technique-lcineBlue staining under acid conditions and PAS reaction(AB-PAS staining). Thekidney appeared one day before hatching, spleen, liver and pancreas emergedon the same day of hatching, and thymus was not found until the third day afterhatching. Ontogeny of these immune related organs of the carp in thisresearch is earlier than that reported by Botham.
文摘This editorial describes the indications and technical aspects of the simultaneous retrieval of thoracic and abdominal organs in Maastricht III donors as well as the preservation of such organs until their implantation.
基金Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province, NO. 2003C130 and NO. 2004C142 Foundation Project for Medical Science and Technology of Zhejiang province, No. 2003B134+3 种基金 Grave Foundation Project for Technological and Development of Hangzhou, No. 2003123B19 Intensive Foundation Project for Technology of Hangzhou, NO. 2004Z006 Foundation Project for Medical Science and Technology of Hangzhou, No. 2003A004 and Foundation Project for Technology of Hangzhou, No. 2005224
文摘AIM: To observe the therapeutic effects of dexamethasone on rats with severe acute pancreatitis (SAP) and investigate the influences of dexamethasone on the inflammatory mediators and NF-κB expression in multiple organs of SAP rats as well as the mechanisms involved. METHODS: Ninety Sprague-Dawley (SD) rats with SAP were randomly divided into the model group (n = 45) and dexamethasone treatment group (n = 45), and another 45 rats were selected for the sham operation group. All groups were randomly subdivided into the 3 h, 6 h and 12 h groups, each group containing 15 rats. The survival of all groups and pathological changes of multiple organs (liver, kidney and lung) were observed at different time points after the operation. The pathologicalscore of multiple organs was carried out, followed by the determination of amylase, endotoxin and TNF-α contents in blood. The tissue microarray was used to detect the expression levels of NF-κB p65 protein in multiple organs. RESULTS: There was no marked difference between the model group and treatment group in the survival rate. The amylase content of the treatment group was significantly lower compared to the model group at 12 h (P < 0.01, 7791.00 vs 9195.00). Moreover, the endotoxin and TNF-α levels of the treatment group were significantly lower than that of the model group at 6 h and 12 h (P < 0.01, 0.040 vs 0.055, 0.042 vs 0.059 and P < 0.05, 58.30 vs 77.54, 38.70 vs 67.30, respectively). Regarding the changes in liver NF-κB expression, the model group significantly exceeded the sham operation group at 3 h (P < 0.01, 1.00 vs 0.00), and the treatment group significantly exceeded the sham operation group at 12 h (P < 0.01, 1.00 vs 0.00), whereas no marked difference was observed between the model group and treatment group at all time points. The kidney NF-κB expression level in the treatment group significantly exceeded the model group (P < 0.05, 2.00 vs 0.00) and the sham operation group (P < 0.01, 2.00 vs 0.00) at 12 h. No NF-κB expression in the lung was found in any group. CONCLUSION: Dexamethasone can lower the amylase, endotoxin and TNF-α levels as well as mortality of SAP rats. NF-κB plays an important role in multiple organ injury. Further studies should be conducted to determine whether dexamethasone can ameliorate the pathological changes of multiple organs by reducing the NF-κB expression in the liver and kidney. The advantages of tissue microarrays in pancreatitis pathological examination include time- and energy- saving, and are highly efficient and representative. The restriction of tissue microarrays on the representation of tissues to various extents due to small diameter may lead to the deviation of analysis.
基金Project (No. 2010382) supported by the Foundation for the Excellent Middle-Aged and Talented Young Persons of Zhejiang Province"151", China
文摘Objective:To investigate the protective effects and mechanisms of action of dexamethasone and Salvia miltiorrhiza on multiple organs in rats with severe acute pancreatitis (SAP). Methods:The rats were divided into sham-operated, model control, dexamethasone treated, and Salvia miltiorrhiza treated groups. At 3, 6, and 12 h after operation, the mortality rate of different groups, pathological changes, Bcl-2-associated X protein (Bax) and nuclear factor-κB (NF-κB) protein expression levels in multiple organs (the pancreas, liver, kidneys, and lungs), toll-like receptor 4 (TLR-4) protein levels (only in the liver), intercellular adhesion molecule 1 (ICAM-1) protein levels (only in the lung), and terminal deoxynucleotidy transferase mediated deoxyuridine triphosphate (dUTP) nick end labeling (TUNEL) staining expression levels, as well as the serum contents of amylase, glutamate-pyruvate transaminase (GPT), glutamic-oxaloacetic transaminase (GOT), blood urea nitrogen (BUN), and creatinine (CREA) were observed. Results:The mortality rate of the dexamethasone treated group was significantly lower than that of the model control group (P<0.05). The pathological changes in multiple organs in the two treated groups were relieved to different degrees (P<0.05 and P<0.01, respectively), the expression levels of Bax and NF-κB proteins, and apoptotic indexes of multiple organs were reduced (P<0.05 and P<0.01, respectively). The contents of amylase, GPT, GOT, BUN, and CREA in the two treated groups were significantly lower than those in model control groups (P<0.05 and P<0.01, respectively). The expression level of ICAM-1 protein in the lungs (at 3 and 12 h) in the dexamethasone treated group was significantly lower than that in the Salvia miltiorrhiza treated group (P<0.05). The serum contents of CREA (at 12 h) and BUN (at 6 h) of the Salvia miltiorrhiza treated group were significantly lower than those in the dexamethasone treated group (P<0.05). Conclusions:Both dexamethasone and Salvia miltiorrhiza can reduce the inflammatory reaction, regulate apoptosis, and thus protect multiple organs of rats with SAP.
文摘Under the environment of an artificial climate chamber, supercooling point （SCP） and freezing point （FP） in flower and young fruit at different development stages and freezing injuries of floral organs were studied. The apricot cultivars tested were Kety, Golden Sun and Honghebao. With the development of flower buds, SCP and FP increased, which indicated that their cold resistance decreased. SCP and FP varied with different floral organs. For different apricot cultivars, it was found that, the lower SCP or FP in floral organs was, the more resistant capacity the cultivar had, and the larger the temperature interval from SCP to FP was. SCP was not a constant value, but a range. Frequency distribution of SCP in petals was more dispersing than that in stamens and pistils. Floral organs could maintain a supercooling state to avoid ice formation, but they were sensitive to freezing. Once floral organs froze, thev turned brown after thawing.
基金supported by grants from the Technological Foundation Project of Traditional Chinese Medicine Science of Zhejiang Province (2003C130) Zhejiang First Level 151Talent Foundation (2010382)
文摘BACKGROUND:Severe acute pancreatitis (SAP) is characterized by fatal pathogenic conditions and a high mortality.It is important to study SAP complicated with multiple organ injury.In this study we compared the protective effects of three traditional Chinese medicines (Ligustrazine,Kakonein and Panax Notoginsenoside) on the small intestine and immune organs (thymus,spleen and lymph nodes) of rats with SAP and explored their mechanism of action.METHODS:One hundred forty-four rats with SAP were randomly divided into model control,Ligustrazine-treated,Kakonein-treated,and Panax Notoginsenoside-treated groups (n=36 per group).Another 36 normal rats comprised the sham-operated group.According to the different time points after operation,the experimental rats in each group were subdivided into 3-,6-and 12-hour subgroups (n=12).At various time points after operation,the mortality rate of rats and pathological changes in the small intestine and immune organs were recorded and the serum amylase levels were measured.RESULTS:Compared to the model control groups,the mortality rates in all treated groups declined and the pathological changes in the small intestine and immune tissues were relieved to different degrees.The serum amylase levels in the three treated groups were significantly lower than those in the model control group at 12 hours.The pathological severity scores for the small intestinal mucosa,thymus and spleen (at 3 and 12 hours) in the Ligustrazine-treated group,for the thymus (at 3 and 12 hours) and spleen (at 3 and 6 hours) in the Kakonein-treated group,and for the thymus (at 3 hours)and spleen (at 3 hours) in the Panax Notoginsenoside-treated group were significantly lower than those in the model control group.The pathological severity scores of the small intestinal mucosa (at 6 and 12 hours) and thymus (at 6 hours) in the Ligustrazine-treated group were significantly lower than those in the Kakonein-and Panax Notoginsenoside-treated groups.CONCLUSIONS:All the three traditional Chinese drugs significantly alleviated the pathological changes in the small intestine and immune organs of SAP rats.Ligustrazine was the most effective one among them.
基金Supported by Sichuan Provincial Program on Basic Research Project(15JC0096)~~
文摘[Objective] This study was conducted to investigate the morphological and physiological differences between the flowers opening spring and late autumn of Hosui Asian pear（Pyrus pyrifolia Nakai）. [Method] Pear flowers were collected from normal bloom（NB） and returning bloom periods during late autumn in 2011-2013,respectively. The morphological and physiological indices including pollen number,germination rate, petal length, soluble protein content, soluble sugar content, amino acid content, pollen tube growth, fruiting characteristics of NB and RB flowers were detected and compared. [Result] The filament length and petal area of RB flowers were significantly smaller than those of NB flowers. The contents of soluble proteins, soluble sugars and amino acids of RB pollens were significantly smaller than those of NB flowers. In addition, the abortion rate of RB flowers was higher than that of NB flowers. [Conclusion] RB flowers had complete floral organs and were capable of pollination, but they were different from NB flowers in some morphological and physiological indices.
文摘Aim: To observe the cytotoxic effect of the organophosphate insecticide malathion in the reproductive tissues of the earthworms, Eisenia foetida. Methods: Worms were nourished in soil treated with malathion at single sub-lethal doses of 0, 80, 150, 300 and 600 mg-kg^(-1) soil. (LD_(50) = 880 mg kg^(-1) soil) and evaluated on days 1, 5, 15 and 30 after exposure. The body weights were recorded and male reproductive organs evaluated. Results: Malathion-treated animals showed a significant reduction in body weight in a dose-dependent manner. Malathion treatment modified the disposition of spermatozoa in the basal epithelium of the spermatheca. The Br-deoxyuridine test showed a significant rise in cells in phase S on days 5 and 15. Also, a higher percentage of spermatogonia with fragmented DNA were observed by means of the TdT-mediated dUTP nick-end labeling (TUNEL) technique in the spermatheca of treated animals. Conclusion: Treatment with malathion decreased the body weight and the spermatic viability in spermatheca, altering the cell proliferation and modifying the DNA structure of spermatogonia.
基金supported by the National Natural Science Foundation of China(NSFC30471016)National Ample Commissariat Program of Technology,China(2004BA520A03,BE2004387).
文摘Based on the experiment of measuring panicles and leaves, air temperature, and humidity above the canopy of rice cultivars after heading in 2005 and 2006, we investigated the temperature difference （TD） between the air and organs of rice plant and its relationship with spikelet fertility. The results showed that TDs between the air and organs of rice varied with air temperature, air humidity, and plant type. For similar air humidity, TDs were lower at the air temperature of 28.5℃ than at higher temperature of 35.5℃, whereas for the same air temperature, the TDs decreased as the air humidity increased. TDs were also affected by plant type of the cultivars. Erect panicle cultivars showed higher TDs than those with droopy panicles under similar climatic conditions, and cultivars with panicles above flag leaf （PAFL） had higher TDs than those with panicles below the flag leaf （PBFL）. Cultivars grown in a location with lower air humidity and higher temperature, such as Taoyuan, China, had higher spikelet fertility than those in higher humidity under the similar air temperature during the grain filling stage. This is partially attributed to the larger TDs under the lower humidity. Rowspacing and the ratio of basal-tillering to panicle-spikelet fertilizer showed a significant influence on TD and subsequently on spikelet fertility, suggesting the possibility of increasing spikelet fertility by agronomic management.
基金The National Key Technology R&D Program of China, No. 2004BA901A03National Scientific and Technical Support Program, No. 2007Z06-017+3 种基金The Cultivation Fund of the Key Scientific and Technical Innovation Project & Ministry of Education of China, No. 706050Program for New Century Excellent Talents in University, No. NCET-04-0906/NCET-06-0818Sichuan Province Basic Research Program, No. 04JY0290061/07JY029-017Program for Key DisciplinesConstruction of Sichuan Province No. SZD0418
文摘AIM： To identify and understand the regular distribution pattern for Salmonella enteritidis （S. enteritidis） in the internal organs of mice after an oral challenge over a 3 wk period. METHODS： Assays based on the serovar-specific DNA sequence of S. enteritidis from GenBank, and a serovar-specific real-time, fluorescence-based quantitative polymerase chain reaction （FQ-PCR） were developed for the detection of S. enteritidis. We used this assay to detect genomic DNA of S. enteritidis in the blood and the internal organs, including heart, liver, spleen, kidney, pancreas, and gallbladder, from mice after oral challenge at different time points respectively.RESULTS： The results showed that the spleen was positive at 12 h post inoculation （PI）, and the blood was at 14 h PI. The organism was detected in the liver and heart at 16 h PI, the pancreas was positive at 20 h PI, and the final organs to show positive results were the kidney and gallbladder at 22 h PI. The copy number of S. enteritidis DNA in each tissue reached a peak at 24-36 h PI, with the liver and spleen containing high concentrations of S. enteritidis, whereas the blood, heart, kidney, pancreas, and gallbladder had low concentrations. S. enteritidis populations began to decrease and were not detectable at 3 d PI, but were still present up to 12 d PI in the gallbladder, 2 wk for the liver, and 3 wk for the spleen without causing apparent symptoms.CONCLUSION： The results provided significant data for understanding the life cycle of S. enteritidis in the internal organs, and showed that the liver and spleen may be the primary sites for setting itself up as a commensa over a long time after oral challenge. Interestingly, it may be the first time reported that the gallbladder is a site of carriage for S. enteritidis over a 12 d period. This study will help to understand the mechanisms of action of S. enteriCdis infection in vivo.
基金Supported by Natural Science Foundation-funded Project Based on the Theory of"Cold Leads to Blood Vessel Collection and Blood Stasis",to Discuss the Characteristics of Microvascular Endothelial Function Changes in the Syndrome of Cold Coagulation and Blood Stasis,and the Influence of TCM(No.81373514)。
文摘OBJECTIVE: To investigate the relationship between symptom patterns of cold coagulation and blood stasis(CCBS) and microcirculation disturbance. In addition, we determined the efficacy of modified Wenjing decoction(WJD) for the treatment of CCBS.METHODS: CCBS was induced in rats with an icewater bath treatment. The ovarian function, microvascular and circulatory status of reproductive organs, and function of local microvascular endothelial cells(VECs) and vascular smooth muscle cells(VSMCs) were evaluated.RESULTS: Ovarian dysfunction was observed in the rats with CCBS. It was characterized by the presence of an estrous cycle disorder and a decrease in reproductive hormone levels. Microvascular circulation disorders were associated with an imbalance in vasoconstriction, relaxation substances, nitric oxide, abnormal blood flow in whole blood, and decreased blood flow in the auricle and uterus. VECs were damaged, and VSMCs contracted and proliferated in ovarian and uterine tissues.CONCLUSION: Our findings suggest that the dysfunctional reproductive organs observed in gynecological CCBS may be closely related to the microcirculation disturbance of local tissues, microvascular contraction, and vascular remodeling. Modified WJD can be used to treat CCBS by improving microcirculation in reproductive organs.
