Mutations and rearrangements of mitochondrial genes cause plant cytoplasmic male sterility. It is a significant way to utilize hybrid vigor to enhance crop yield. Ogu cytoplasmic male sterility(CMS) is a natural cytop...Mutations and rearrangements of mitochondrial genes cause plant cytoplasmic male sterility. It is a significant way to utilize hybrid vigor to enhance crop yield. Ogu cytoplasmic male sterility(CMS) is a natural cytoplasmic male sterility type discovered in radishes, being successfully transferred to rapeseed and cruciferous vegetables. However, current studies lack depth in exploring the molecular mechanisms of its male sterility. This study confirmed that orf138 is the causal gene for Ogu CMS through the genetic transformation in Arabidopsis. Transcriptome analysis of aborted anthers in different stages suggested that differentially expressed genes(DEGs) are mainly enriched in pathways such as glycerophospholipid metabolism and arginine and proline metabolism. It reveals that key genes involved in lipid metabolism pathways are significantly down-regulated in the sterile line(OguA), including BnaGPAT1, localized within the tapetum mitochondrial and endoplasmic reticulum. This could lead to changes in the metabolism of substances like acylglycerols within the tapetum, causing disruptions in lipid metabolism. This is consistent with morphological and subcellular structural changes in the tapetum and microspore cells, as observed in the transmission electron microscopy. This abnormal lipid metabolism may trigger specific reactive oxygen species(ROS) accumulation in an oxidative stress response, ultimately leading to an aborted microspore. Our study based on transcriptome has deepened our understanding of the molecular mechanisms in Ogu CMS.展开更多
Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(...Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(CRC).Methods We constructed a protein-protein interaction network of C8orf76-related genes and analyzed the prognostic signatures of C8orf76 and CDK4.Clinicopathological features of C8orf76 and CDK4 were visualized using a nomogram.Results C8orf76 and CDK4 levels were positively correlated in two independent human CRC cohorts(n=83 and n=597).A consistent positive correlation was observed between C8orf76 and CDK4 expression in the CRC cell lines.The nomogram included prognostic genes(C8orf76 and CDK4)and pathological N and M stages.The concordance index(C-index)in our cohort was 0.776,which suggests that the ability of the indicators to predict the overall survival of patients with CRC in our cohort was strong.Conclusion We found that C8orf76 was positively correlated with CDK4 in both the cohorts as well as in CRC cell lines.Therefore,C8orf76 and CDK4 can be used as potential biomarkers to predict the prognosis of CRC.展开更多
基金supported by the National Natural Science Foundation of China (31930032)。
文摘Mutations and rearrangements of mitochondrial genes cause plant cytoplasmic male sterility. It is a significant way to utilize hybrid vigor to enhance crop yield. Ogu cytoplasmic male sterility(CMS) is a natural cytoplasmic male sterility type discovered in radishes, being successfully transferred to rapeseed and cruciferous vegetables. However, current studies lack depth in exploring the molecular mechanisms of its male sterility. This study confirmed that orf138 is the causal gene for Ogu CMS through the genetic transformation in Arabidopsis. Transcriptome analysis of aborted anthers in different stages suggested that differentially expressed genes(DEGs) are mainly enriched in pathways such as glycerophospholipid metabolism and arginine and proline metabolism. It reveals that key genes involved in lipid metabolism pathways are significantly down-regulated in the sterile line(OguA), including BnaGPAT1, localized within the tapetum mitochondrial and endoplasmic reticulum. This could lead to changes in the metabolism of substances like acylglycerols within the tapetum, causing disruptions in lipid metabolism. This is consistent with morphological and subcellular structural changes in the tapetum and microspore cells, as observed in the transmission electron microscopy. This abnormal lipid metabolism may trigger specific reactive oxygen species(ROS) accumulation in an oxidative stress response, ultimately leading to an aborted microspore. Our study based on transcriptome has deepened our understanding of the molecular mechanisms in Ogu CMS.
基金supported by a grant from National Natural Science Foundation of China(No.82203623)2022&2023 Hebei introduction of foreign expert intelligence projects(Nos.YZ202201&YZ202305)+1 种基金Hebei Natural Science Foundation(Nos.H2020206374 and H2021206306)Hebei Clinical Medicine Excellent Talents Project of Province(No.LS202001).
文摘Objective To explore the correlation between chromosome 8 open reading frame 76(C8orf76)and cyclin-dependent kinase 4(CDK4)and the potential predictive effect of C8orf76 and CDK4 on the prognosis of colorectal cancer(CRC).Methods We constructed a protein-protein interaction network of C8orf76-related genes and analyzed the prognostic signatures of C8orf76 and CDK4.Clinicopathological features of C8orf76 and CDK4 were visualized using a nomogram.Results C8orf76 and CDK4 levels were positively correlated in two independent human CRC cohorts(n=83 and n=597).A consistent positive correlation was observed between C8orf76 and CDK4 expression in the CRC cell lines.The nomogram included prognostic genes(C8orf76 and CDK4)and pathological N and M stages.The concordance index(C-index)in our cohort was 0.776,which suggests that the ability of the indicators to predict the overall survival of patients with CRC in our cohort was strong.Conclusion We found that C8orf76 was positively correlated with CDK4 in both the cohorts as well as in CRC cell lines.Therefore,C8orf76 and CDK4 can be used as potential biomarkers to predict the prognosis of CRC.
