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Mfn-1、Mfn-2和Opa1基因多态性与梅州客家人群原发性高血压严重程度及临床疗效的关系 被引量:1
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作者 杨敏 林佑妮 +3 位作者 蔡裕福 石文坚 陈玉宽 李明瑞 《中南医学科学杂志》 2025年第1期149-153,共5页
目的分析线粒体融合蛋白-1(Mfn-1)、Mfn-2和视神经萎缩蛋白1(Opa1)基因多态性与梅州客家人群原发性高血压严重程度及临床疗效的关系。方法选择收治的梅州客家100例原发性高血压患者为观察组,同期选择健康客家人群46例为对照组。比较两... 目的分析线粒体融合蛋白-1(Mfn-1)、Mfn-2和视神经萎缩蛋白1(Opa1)基因多态性与梅州客家人群原发性高血压严重程度及临床疗效的关系。方法选择收治的梅州客家100例原发性高血压患者为观察组,同期选择健康客家人群46例为对照组。比较两组受检者临床资料,包括血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、空腹血糖(FBG)、糖化血红蛋白(HbA1c)、血尿酸(UA)、血肌酐(Cr)和尿素氮(UN)等生化指标。采用单核苷酸多态性位点(SNP)多态性测序检测Mfn-1、Mfn-2和Opa1基因多态性,比较观察组及对照组Mfn-1(rs11916762、rs7620017)、Mfn-2(rs2336384、rs2236057)及Opa1(rs7620342、rs11925699)SNPs的分布情况;进而分析观察组中不同高血压分级及临床疗效亚组Mfn-1、Mfn-2和Opa1基因SNPs的分布情况。结果观察组体质指数、TC、LDL、UA及Cr水平高于对照组(P<0.05)。观察组与对照组患者基因多态性位点rs11916762、rs7620017 AG、rs2336384 TT、rs2236057 GG、rs7620342 GT及rs11925699 AA+AG分布占比差异无统计学意义(P>0.05)。在不同分级的原发性高血压亚组患者中3级原发性高血压患者rs2336384 GG占比高,且GT及TT占比低于1级原发性高血压组(P<0.05)。治疗显效患者rs11916762和rs7620017 AG、rs2336384 TT、rs2236057 GG、rs7620342 GT及rs11925699 AA+AG分布占比高于无效患者(P<0.05);而rs11916762和rs7620017 AA及GG,rs2336384 GG及GT,rs2236057 AA,rs7620342 GG及TT,rs11925699 AA+GG占比低于无效患者(P<0.05)。结论Mfn-1、Mfn-2及Opa1基因多态性在梅州客家人群原发性高血压不同分级及临床疗效亚组中分布不同,可用来指导临床优化原发性高血压个体化药物治疗方案,进而为早期开展相应的防治措施提供有效依据。 展开更多
关键词 Mfn-1 Mfn-2 opa1 基因多态性 梅州客家人群 原发性高血压 临床疗效
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强心汤调控p32/OMA1/OPA1通路维持线粒体功能改善慢性心力衰竭小鼠心肌损伤和纤维化 被引量:1
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作者 毛美玲 卢健棋 +6 位作者 潘朝锌 朱智德 卢俊燊 卢洁 谢丽钰 陈佳永 肖湘 《药物评价研究》 北大核心 2025年第1期51-59,共9页
目的探讨强心汤调控p32/OMA1/OPA1通路介导的线粒体功能防治慢性心力衰竭纤维化的作用机制。方法除7只对照组小鼠外,余33只小鼠通过结扎冠状动脉建立慢性心力衰竭小鼠模型,并将存活的28只小鼠随机分为模型组、沙库巴曲缬沙坦(阳性药,15.... 目的探讨强心汤调控p32/OMA1/OPA1通路介导的线粒体功能防治慢性心力衰竭纤维化的作用机制。方法除7只对照组小鼠外,余33只小鼠通过结扎冠状动脉建立慢性心力衰竭小鼠模型,并将存活的28只小鼠随机分为模型组、沙库巴曲缬沙坦(阳性药,15.17 mg·kg^(-1))组及强心汤低、高剂量(21.69、43.38 g·kg^(-1))组,每组7只,对照组、模型组给予等量纯净水,造模后每天ig给药1次,连续4周。进行苏木素-伊红(HE)、Masson染色观察各组小鼠心肌组织病理损伤和纤维化情况;透射电镜观察线粒体损伤情况;免疫组化法和Western blotting法检测心脏组织中补体成分1q结合蛋白(p32)、线粒体内膜蛋白1(OMA1)、视神经萎缩蛋白1(OPA1)的表达情况;生化检测法测定各组小鼠血清中三磷酸腺苷(ATP)含量。结果与对照组比较,模型组小鼠心肌组织损伤严重、心肌纤维化,线粒体碎裂、嵴消失;p32、长链OPA1(L-OPA1)蛋白表达显著降低(P<0.01),OMA1、短链OPA1(S-OPA1)蛋白表达显著升高(P<0.01),血清ATP浓度水平显著降低(P<0.01)。与模型组比较,强心汤高剂量组小鼠心肌组织整齐、纤维化明显改善,p32、L-OPA1蛋白表达显著增加(P<0.01),OMA1、S-OPA1蛋白表达显著降低(P<0.01),血清ATP含量明显增加(P<0.01)。结论强心汤能够积极调控p32/OMA1/OPA1通路发挥维持线粒体供能、减轻慢性心力衰竭模型小鼠心肌损伤和组织纤维化的作用。 展开更多
关键词 强心汤 慢性心力衰竭 p32/OMA1/opa1 线粒体 心肌损伤 纤维化
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A novel one-time-programmable memory unit based on Schottky-type p-GaN diode
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作者 Chao Feng Xinyue Dai +4 位作者 Qimeng Jiang Sen Huang Jie Fan Xinhua Wang Xinyu Liu 《Journal of Semiconductors》 EI CAS CSCD 2024年第3期53-57,共5页
In this work,a novel one-time-programmable memory unit based on a Schottky-type p-GaN diode is proposed.During the programming process,the junction switches from a high-resistance state to a low-resistance state throu... In this work,a novel one-time-programmable memory unit based on a Schottky-type p-GaN diode is proposed.During the programming process,the junction switches from a high-resistance state to a low-resistance state through Schottky junction breakdown,and the state is permanently preserved.The memory unit features a current ratio of more than 10^(3),a read voltage window of 6 V,a programming time of less than 10^(−4)s,a stability of more than 108 read cycles,and a lifetime of far more than 10 years.Besides,the fabrication of the device is fully compatible with commercial Si-based GaN process platforms,which is of great significance for the realization of low-cost read-only memory in all-GaN integration. 展开更多
关键词 wide-bandgap semiconductor one-time programmable Schottky-type p-GaN diode read-only memory device
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OPA1在胃癌组织中的表达及临床意义研究
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作者 殷利军 宋琳琳 《宁夏医学杂志》 2025年第9期745-749,F0002,共6页
目的通过对癌症基因组图谱(TCGA)数据库与基因表达综合(GEO)数据库中胃癌(GC)的数据挖掘,探讨线粒体融合蛋白1(OPA1)在GC中的表达及临床意义。方法通过TCGA和GEO数据库分析OPA1在GC组织中及癌旁组织的表达差异,在R4.4.1版本软件中应用gg... 目的通过对癌症基因组图谱(TCGA)数据库与基因表达综合(GEO)数据库中胃癌(GC)的数据挖掘,探讨线粒体融合蛋白1(OPA1)在GC中的表达及临床意义。方法通过TCGA和GEO数据库分析OPA1在GC组织中及癌旁组织的表达差异,在R4.4.1版本软件中应用ggpubr包绘制箱线图对OPA1基因正常组和胃癌肿瘤组表达差异进行可视化及统计学差异分析。利用GSE236522中胃癌样本的生存数据,使用survminer包进行比例风险假设检验并拟合生存曲线,评估它们的生存作用和功能。采用实时定量聚合酶链反应(RT-qPCR)法和蛋白质印迹法(Western blot)检测GC组织中OPA1基因和蛋白的表达水平。利用TCGA数据库的胃腺癌(STAD)组织与正常组织中经limma包分析得到的差异基因1,STAD组织中OPA1高表达与低表达所得的差异基因2进行比较,得出交集差异基因,对交集差异基因进行GO功能注释和KEGG通路分析。结果GC癌组织中OPA1蛋白表达阳性率高于癌旁组织,差异有统计学意义(P<0.05)。RT-qPCR结果显示,与癌旁组织相比,OPA1在GC组织中表达显著增高(P<0.05)。OPA1高表达组GC患者生存率低于OPA1低表达的患者。结论GC癌组织中OPA1蛋白表达升高,且表达水平与GC病理特征有关,诊断预测敏感度高,可能成为未来预测GC患者不良生存预后的潜在肿瘤标志物。 展开更多
关键词 胃癌 opa1 生物信息学 临床意义
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Evaluation of the Effectiveness and Safety of One-Time Endodontics in the Treatment of Chronic Apical Periodontitis with Sinus Tract in Pediatric Deciduous Teeth
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作者 Yi Liu 《Journal of Clinical and Nursing Research》 2024年第6期111-116,共6页
Objective:To observe the effectiveness and safety of one-time endodontics in the treatment of chronic apical periodontitis with sinus tract in pediatric deciduous teeth.Methods:109 cases of children with chronic apica... Objective:To observe the effectiveness and safety of one-time endodontics in the treatment of chronic apical periodontitis with sinus tract in pediatric deciduous teeth.Methods:109 cases of children with chronic apical periodontitis with sinus tract in the deciduous teeth treated in our hospital from January 2022 to December 2023 were selected and grouped by the randomized numerical table method,with 54 cases in the experimental group and 55 cases in the control group.The experimental group was treated with one-time endodontics and the control group was treated with conventional endodontics.Results:After the treatment,the total effective rate of treatment was higher in the experimental group than in the control group(P<0.05);the incidence of adverse events was lower in the experimental group than in the control group(P<0.05);the satisfaction of the children's family members was higher in the experimental group than in the control group(P<0.05);the pain duration was lower in the experimental group than in the control group(P<0.05).Conclusion:In the experimental group,children with chronic apical periodontitis with sinus tract of the deciduous teeth were given one-time endodontic treatment,and the results of its implementation were relatively good. 展开更多
关键词 one-time endodontics Chronic apical periodontitis with sinus tract Pediatric deciduous teeth Therapeutic effectiveness SAFETY
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Fem-1同系物C通过调节ELAVL1/OPA1轴介导的线粒体融合促进乳腺癌进展的机制研究
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作者 樊艳 栗粟 +3 位作者 田天 杨萌萌 张璐璐 赵素贞 《中国肿瘤临床》 北大核心 2025年第10期487-493,共7页
目的:探讨Fem-1同系物C(Fem-1 homolog C,FEM1C)对乳腺癌的影响及其潜在调节机制。方法:通过qPCR检测乳腺癌组织和细胞系中FEM1C的表达水平。利用在线数据库预测FEM1C与ELAVL1蛋白的结合,并通过CoIP分析进行验证;利用starBase数据库预测... 目的:探讨Fem-1同系物C(Fem-1 homolog C,FEM1C)对乳腺癌的影响及其潜在调节机制。方法:通过qPCR检测乳腺癌组织和细胞系中FEM1C的表达水平。利用在线数据库预测FEM1C与ELAVL1蛋白的结合,并通过CoIP分析进行验证;利用starBase数据库预测ELAVL1与OPA1 mRNA的结合,并通过RIP分析进行验证。采用FEM1C的shRNA(sh-FEM1C)和过表达载体(FEM1C)、ELAVL1的过表达载体(ELAVL1)、OPA1的过表达载体(OPA1)转染乳腺癌细胞MDA-MB-231,或用100μM的DRP1抑制剂Mdivi-1或OPA1抑制剂MYLS22处理MDA-MB-231细胞。用sh-FEM1C慢病毒载体注射裸鼠构建异种移植瘤模型,监测肿瘤生长。结果:乳腺癌组织中FEM1C表达显著上调(P<0.01)。沉默FEM1C抑制MDA-MB-231细胞增殖,诱导细胞凋亡,促进自噬蛋白LC3Ⅱ/Ⅰ表达,抑制p62表达,上调线粒体PINK1蛋白水平,促进线粒体裂变蛋白DRP1和MIEF2表达,抑制融合蛋白OPA1和MFN1表达(P<0.01)。Mdivi-1处理抑制DRP1表达(P<0.01),对细胞活力无影响(P>0.05);MYLS22处理抑制OPA1表达,抵消FEM1C过表达对MDA-MB-231细胞的影响(P<0.01)。机制研究显示FEM1C与ELAVL1蛋白结合,并促进其表达(P<0.01);ELAVL1蛋白通过与OPA1 mRNA结合稳定其mRNA,上调OPA1蛋白水平(P<0.01)。过表达OPA1逆转了FEM1C沉默对乳腺癌细胞的影响(P<0.01)。体内结果显示,敲低FEM1C抑制体内肿瘤生长(P<0.01)。结论:FEM1C通过促进ELAVL1蛋白表达介导OPA1 mRNA稳定性,从而促进线粒体融合,抑制自噬,促进乳腺癌进展。 展开更多
关键词 乳腺癌 FEM1C ELAVL1 opa1 线粒体融合
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长链非编码RNA Opa相互作用蛋白5反义RNA1在胰腺癌中的表达及其与临床病理特征及预后的关系分析
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作者 刘尧 周文聪 黄耀星 《中外医药研究》 2025年第27期148-150,共3页
目的:探讨长链非编码RNA(LncRNA)Opa相互作用蛋白5反义RNA1(OIP5-AS1)在胰腺癌患者中的表达及其与临床病理特征及预后的关系。方法:选取2022年9月—2023年9月在广州市第一人民医院接受外科手术治疗的60例胰腺癌患者的癌组织样本。比较... 目的:探讨长链非编码RNA(LncRNA)Opa相互作用蛋白5反义RNA1(OIP5-AS1)在胰腺癌患者中的表达及其与临床病理特征及预后的关系。方法:选取2022年9月—2023年9月在广州市第一人民医院接受外科手术治疗的60例胰腺癌患者的癌组织样本。比较胰腺癌组织和癌旁组织中LncRNA OIP5-AS1表达水平。根据胰腺癌组织中LncRNA OIP5-AS1表达均值,将患者分为高表达组(n=22)和低表达组(n=38)。采用Spearman相关分析LncRNA OIP5-AS1表达与患者临床病理特征的关系,Kaplan-Meier法分析LncRNA OIP5-AS1表达与预后的关系。结果:胰腺癌组织中LncRNA OIP5-AS1水平高于癌旁组织(P<0.001)。两组患者在分化程度、TNM分期、肿瘤直径、淋巴结转移方面比较,差异有统计学意义(P<0.05)。LncRNA OIP5-AS1表达与分化程度呈负相关(P=0.003),与TNM分期、肿瘤直径、淋巴结转移呈正相关(P<0.05)。LncRNA OIP5-AS1低表达患者总生存率和无疾病进展生存率高于LncRNA OIP5-AS1高表达患者(P<0.05)。结论:胰腺癌组织中LncRNA OIP5-AS1表达异常升高,与患者肿瘤分化程度、TNM分期等病理特征相关,且高表达患者预后更差。 展开更多
关键词 胰腺癌 长链非编码RNA opa相互作用蛋白5反义RNA1 病理特征 预后
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基于OPA-熵权组合赋权法和TOPSIS法的配电网工程投资能力评价模型构建
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作者 左帆 丁灵卫 《电力设备管理》 2025年第17期99-101,共3页
电网公司作为电力系统的重要组成部分,其投资能力的科学评价对于确保电力系统的可靠性和经济性具有重要意义。为准确评价配电网工程的投资能力,科学指导投资决策和投资安排,提出了一种OPA-熵权组合赋权法和TOPSIS法的投资能力评价模型... 电网公司作为电力系统的重要组成部分,其投资能力的科学评价对于确保电力系统的可靠性和经济性具有重要意义。为准确评价配电网工程的投资能力,科学指导投资决策和投资安排,提出了一种OPA-熵权组合赋权法和TOPSIS法的投资能力评价模型。从财务保障能力、建设能力、管理能力构建配电网工程投资能力评价指标体系,然后采用OPA法和熵权法组合赋权确定各指标权重,最后基于TOPSIS逼近理想解排序法评价配电网工程投资能力。通过对投资能力的量化研究,掌握投资能力变化趋势,挖掘关键影响因素,为决策者提供科学的参考依据。 展开更多
关键词 配电网 投资能力 opa 熵权法 TOPSIS
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基于模糊OPA方法的城市技术网络融合水平研究
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作者 李凯帅 《信息系统工程》 2025年第4期117-120,共4页
旨在基于专利引用数据,通过构建城市间技术融合网络,利用复杂网络分析方法探究不同城市间的技术合作关系,揭示城市在技术合作网络中的作用与结构特征。同时,创新性地将模糊OPA(Ordinal Priority Approach)方法引入城市技术融合评价,解... 旨在基于专利引用数据,通过构建城市间技术融合网络,利用复杂网络分析方法探究不同城市间的技术合作关系,揭示城市在技术合作网络中的作用与结构特征。同时,创新性地将模糊OPA(Ordinal Priority Approach)方法引入城市技术融合评价,解决多指标权重分配的不确定性问题,希望能够更为全面地评估各城市在技术融合网络中的地位与贡献。结果表明,技术合作网络具有显著的集中性和分层特征,少数核心城市在技术合作中占据主导地位,发挥着重要的桥梁作用,而部分城市技术合作能力不足。 展开更多
关键词 城市技术网络 融合发展 复杂网络分析 模糊opa方法
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大概念统摄下初中地理“OPA进阶式”备考单元范式的构建与实施——以“河流”单元为例
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作者 陈莹莹 陈婷婷 《福建基础教育研究》 2025年第4期86-90,共5页
传统备考模式重知识轻素养、重过程轻结果,难以满足中考改革背景下聚焦学生解决真实情境问题的素养培育要求。为此,提出以地理大概念统摄,构建有序化(Organize)、程序化(Present)和素养化(Apply)的初中地理OPA进阶式备考单元范式,助推... 传统备考模式重知识轻素养、重过程轻结果,难以满足中考改革背景下聚焦学生解决真实情境问题的素养培育要求。为此,提出以地理大概念统摄,构建有序化(Organize)、程序化(Present)和素养化(Apply)的初中地理OPA进阶式备考单元范式,助推学生的能力进阶与素养的教学落地。在此基础上,以“河流”单元为例,从概念提取、单元构建、进阶复习设计等方面,具体阐述该范式的构建与实施过程。 展开更多
关键词 初中地理 大概念 opa进阶式备考单元 中考复习
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扬州群发光芯科技有限公司 OPA硅光芯片驱动全固态激光雷达技术革新
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作者 王家俊 《中国高新科技》 2025年第6期5-7,共3页
在扬州高新技术产业开发区,一家年轻的科技企业正以颠覆性的技术改写激光雷达行业的竞争版图。扬州群发光芯科技有限公司成立于2021年11月,作为中美民间科技合作的典范,与美国密歇根大学展开合作,聚焦全固态光学相控阵(OPA)硅光芯片的... 在扬州高新技术产业开发区,一家年轻的科技企业正以颠覆性的技术改写激光雷达行业的竞争版图。扬州群发光芯科技有限公司成立于2021年11月,作为中美民间科技合作的典范,与美国密歇根大学展开合作,聚焦全固态光学相控阵(OPA)硅光芯片的研发、生产与商业化。公司以OPA技术为核心,逐步打造立足扬州、辐射江苏、影响全国的硅光企业,致力于通过技术创新推动固态OPA激光雷达在自动驾驶、机器人、无人机、3D测绘、安防勘探等诸多领域的普及应用。 展开更多
关键词 opa硅光芯片 扬州群发光芯科技有限公司
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One-time fertilization at first flowering improves lint yield and dry matter partitioning in late planted short-season cotton 被引量:9
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作者 LUO Hong-hai WANG Qiang +3 位作者 ZHANG Jie-kun WANG Lei-shan LI Ya-bing YANG Guo-zheng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2020年第2期509-517,共9页
Cotton producers have substantially reduced their inputs(labor,nutrients,and management)mainly by adopting a shortseason cropping management that is characterized by late sowing,high density,and reduced fertilization ... Cotton producers have substantially reduced their inputs(labor,nutrients,and management)mainly by adopting a shortseason cropping management that is characterized by late sowing,high density,and reduced fertilization with one-time application at the first bloom stage without lint yield reduction.However,it has been hypothesized that one-time fertilization at an earlier growth stage could be a more effective and economic management practice.A two-year field experiment was conducted by applying five fertilizer one-time fertilization at 0(FT1),5(FT2),10(FT3),15(FT4),and 20(FT5)days after the first flower appeared in the field and one three-split fertilizer application taken as the conventional control(FT6),making six treatments altogether.Cotton growth period,biomass accumulation,yield,and its formation were quantified.The results showed that the one-time fertilization did not affect the cotton growth progress as compared to FT6,however,the total crop cycles for FT3–FT5 were 3 days shorter.FT1 produced the highest cotton lint yield(1396 kg ha–1),which was similar to the FT6 but higher than the other treatments,and could be attributed to more bolls per unit area and higher lint percentage.Cotton yield was positively correlated with cotton plant biomass accumulated.FT1 had both the highest average(VT)(193.7 kg ha–1 d–1)and the highest maximum(VM)(220.9 kg ha–1 d–1)rates during the fast biomass accumulation period.These results suggest that one-time fertilizer application at the first flower stage might be an adjustment that is more effective than at first bloom,and allowed for easier decision making for application date due to non counting of plants with flowers is needed. 展开更多
关键词 one-time fertilization first flower cotton biomass accumulation yield
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One-Time Rational Secret Sharing Scheme Based on Bayesian Game 被引量:8
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作者 TIAN Youliang MA Jianfeng +2 位作者 PENG Changgen CHEN Xi JI Wenjiang 《Wuhan University Journal of Natural Sciences》 CAS 2011年第5期430-434,共5页
The rational secret sharing cannot be realized in the case of being played only once, and some punishments in the one-time rational secret sharing schemes turn out to be empty threats. In this paper, after modeling 2-... The rational secret sharing cannot be realized in the case of being played only once, and some punishments in the one-time rational secret sharing schemes turn out to be empty threats. In this paper, after modeling 2-out-of-2 rational secret sharing based on Bayesian game and considering different classes of protocol parties, we propose a 2-out-of-2 secret sharing scheme to solve cooperative problem of a rational secret sharing scheme being played only once. Moreover, we prove that the strategy is a perfect Bayesian equilibrium, adopted only by the parties in their decision-making according to their belief system (denoted by the probability distribution) and Bayes rule, without requiring simultaneous channels. 展开更多
关键词 rational secret sharing one-time rational secret sharing Bayesian game perfect Bayesian equilibrium
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丹参保心茶调节AMPK/OPA1通路对冠心病合并认知功能障碍模型大鼠的影响 被引量:1
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作者 黄怡 杜雨欣 +6 位作者 康舒悦 郑开荣 郑国扬 黄水明 潘华峰 李伟荣 姚丽梅 《中药新药与临床药理》 CAS CSCD 北大核心 2024年第10期1542-1551,共10页
目的探讨丹参保心茶(Danshen Baoxin Cha)对冠心病合并认知功能障碍大鼠的影响。方法将雄性SD大鼠随机分为正常组和模型复制组。模型复制组大鼠双侧脑室注射链脲佐菌素(STZ)造认知功能障碍模型,皮下注射盐酸异丙肾上腺素(ISO)造心肌缺... 目的探讨丹参保心茶(Danshen Baoxin Cha)对冠心病合并认知功能障碍大鼠的影响。方法将雄性SD大鼠随机分为正常组和模型复制组。模型复制组大鼠双侧脑室注射链脲佐菌素(STZ)造认知功能障碍模型,皮下注射盐酸异丙肾上腺素(ISO)造心肌缺血模型。通过行为学实验验证心肌缺血模型及认知功能障碍造模成功后,将模型复制组大鼠随机分为模型组、通心络胶囊组(1.6 g·kg^(-1))及丹参保心茶低(4 g·kg^(-1))、中(8 g·kg^(-1))、高(16 g·kg^(-1))剂量组,连续给药2周。采用Y迷宫、新物体识别实验、Morris水迷宫实验检测大鼠的学习和记忆能力;采用试剂盒检测大鼠脑内氧化应激水平和线粒体中三磷酸腺苷(ATP)含量;采用苏木精-伊红(HE)染色法和Nissl染色法观察海马CA1区神经元的病理变化;采用电镜观察海马CA1区线粒体的病理变化;通过实时荧光定量PCR法检测过氧化物酶体增生物激活受体γ共激活因子1α(PGC-1α)、葡萄糖转运蛋白4(GLUT4)、视神经萎缩蛋白1(OPA1)的表达水平;通过Western Blot检测AMPK/OPA1信号通路相关蛋白的表达量。结果与正常组比,模型组自主交替率、识别指数、穿越原平台区域的次数和路程比明显降低(P<0.01);海马CA1区神经元密度下降,尼氏小体减少,胞核固缩增加;线粒体中ATP和脑组织中ATP、SOD、GSH-PX水平明显降低(P<0.05,P<0.01),ROS、MDA含量明显增加(P<0.05,P<0.01);海马CA1区线粒体肿胀,嵴稀疏,呈空泡化;GLUT4、PGC-1α、OPA1mRNA表达水平均明显降低(P<0.01);GLUT4、SIRT1、PGC-1α、OPA1的蛋白表达水平和p-AMPK/AMPK比值明显降低(P<0.05,P<0.01)。与模型组比,丹参保心茶给药组大鼠的行为学指标明显改善(P<0.05,P<0.01),海马CA1区神经元数量、尼氏小体、核固缩改善;线粒体中ATP和脑组织中ATP、SOD、GSH-PX水平明显升高(P<0.05,P<0.01),ROS、MDA水平明显降低(P<0.05,P<0.01);海马CA1区线粒体嵴结构较完整;GLUT4、PGC-1α、OPA1mRNA表达水平改善(P<0.05,P<0.01);AMPK/OPA1信号通路相关蛋白表达量明显升高(P<0.05,P<0.01)。结论丹参保心茶可以改善冠心病合并认知功能障碍模型大鼠的学习、记忆能力,减轻神经元损伤,其机制可能与减轻脑部氧化应激损伤,激活AMPK/OPA1信号通路,恢复能量水平有关。 展开更多
关键词 丹参保心茶 冠心病 认知功能障碍 氧化应激 能量代谢 AMPK/opa1信号通路 大鼠
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One-time sintering process to modify xLi2MnO3(1-x)LiMO2 hollow architecture and studying their enhanced electrochemical performances 被引量:3
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作者 Renheng Wang Yiling Sun +5 位作者 Kaishuai Yang Junchao Zheng Yan Li Zhengfang Qian Zhenjiang He Shengkui Zhong 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2020年第11期271-279,共9页
To solve the critical problems of lithium rich cathode materials, e.g., structure instability and short cycle life, we have successfully prepared a ZrO2-coated and Zr-doping xLi2MnO3·(1–x)LiMO2 hollow architectu... To solve the critical problems of lithium rich cathode materials, e.g., structure instability and short cycle life, we have successfully prepared a ZrO2-coated and Zr-doping xLi2MnO3·(1–x)LiMO2 hollow architecture via one-time sintering process. The modified structural materials as lithium-ion cathodes present good structural stability and superior cycle performance in LIBs. The discharge capacity of the ZrO2-coated and Zr-doped hollow pristine is 220 mAh g-1 at the 20th cycle at 0.2 C(discharge capacity loss, 2.7%)and 150 m Ah g-1 at the 100 th cycle at 1 C(discharge capacity loss, 17.7%), respectively. However, hollow pristine electrode only delivers 203 m Ah g-1 at the 20 th cycle at 0.2 C and 124 mAh g-1 at the 100 th cycle at 1 C, respectively, and the corresponding to capacity retention is 92.2% and 72.8%, respectively.Diffusion coefficients of modified hollow pristine electrode are much higher than that of hollow pristine electrode after 100 cycles(approach to 1.4 times). In addition, we simulate the adsorption reaction of HF on the surface of ZrO2-coated layer by the first-principles theory. The calculations prove that the adsorption energy of HF on the surface of ZrO2-coated layer is about-1.699 e V, and the ZrO2-coated layer could protect the hollow spherical xLi2MnO3·(1–x)LiMO2 from erosion by HF. Our results would be applicable for systematic amelioration of high-performance lithium rich material for anode with the respect of practical application. 展开更多
关键词 Lithium rich cathode materials one-time sintering process Coated and doped Electrochemical performances First-principles calculations
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基于OPA847和OPA657跨阻放大的平衡光电探测器 被引量:2
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作者 王鼎康 武晋泽 +1 位作者 宋志刚 李晋红 《量子光学学报》 北大核心 2024年第4期108-118,共11页
OPA847和OPA657是Texas Instruments公司生产的两款广泛用于研制平衡光电探测器的高速、低噪声运算放大器。本文研究了基于OPA847和OPA657跨阻放大平衡光电探测器的带宽和噪声特性。在理论上计算了探测器的-3 d B带宽和等效噪声功率(Noi... OPA847和OPA657是Texas Instruments公司生产的两款广泛用于研制平衡光电探测器的高速、低噪声运算放大器。本文研究了基于OPA847和OPA657跨阻放大平衡光电探测器的带宽和噪声特性。在理论上计算了探测器的-3 d B带宽和等效噪声功率(Noise Equivalent Power,NEP)对跨阻增益的依赖关系。OPA847在跨阻增益较小(带宽较大)时给出更小的NEP,而OPA657在跨阻增益较大(带宽较小)时给出更小的NEP。在实验上实现了跨阻增益分别为2 k V/A和200 k V/A的平衡光电探测器,并测量了探测器的频率和噪声性能。跨阻增益为2 k V/A时,OPA847和OPA657分别给出了220 MHz和130 MHz的-3 d B带宽以及8.2pw√Hz和22pw√Hz的NEP。跨阻增益为200 k V/A时,两者分别给出了9.2 MHz和8.5 MHz的-3 d B带宽以及4.3pw√Hz和2.3pw√Hz的NEP。这四种配置的探测器在-3 d B带宽范围内都实现了大于30 d B的共模抑制比。最后,测量了这些探测器在不同本振光功率下的噪声功率谱,在饱和功率范围内都实现了高于电子学噪声10 d B的散粒噪声测量。 展开更多
关键词 平衡光电探测器 跨阻放大器 opa847 opa657
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Cryptanalysis of the One-Time Proxy Signature Scheme Used in Mobile Agents 被引量:1
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作者 FUXiao-tong XIAOHong XIAOGuo-zhen 《Wuhan University Journal of Natural Sciences》 EI CAS 2005年第1期183-185,共3页
We ayptanalyze Kim et. al's one-time proxy signature scheme used in mobileagents, and then a successful forgery is introduced It is showed that a dishonest customer cansuccessfully forge a valid one-time proxy sig... We ayptanalyze Kim et. al's one-time proxy signature scheme used in mobileagents, and then a successful forgery is introduced It is showed that a dishonest customer cansuccessfully forge a valid one-time proxy signature by impersonating the stiver Furthermore, he canrequest the server with responsibility for the forged bidding information. 展开更多
关键词 CRYPTANALYSIS proxy signature one-time proxy signature
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Design of 32 kbit one-time programmable memory for microcontroller units 被引量:1
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作者 JEON Hwang-gon CHOI In-hwa +1 位作者 HA Pan-bong KIM Young-hee 《Journal of Central South University》 SCIE EI CAS 2012年第12期3475-3483,共9页
A 32 kbit OTP(one-time programmable)memory for MCUs(micro-controller units)used in remote controllers was designed.This OTP memory is used for program and data storage.It is required to apply 5.5V to BL(bit-line)and 1... A 32 kbit OTP(one-time programmable)memory for MCUs(micro-controller units)used in remote controllers was designed.This OTP memory is used for program and data storage.It is required to apply 5.5V to BL(bit-line)and 11V to WL(word-line)for a OTP cell of 0.35μm ETOX(EEPROM tunnel oxide)type by MagnaChip.We use 5V transistors on column data paths to reduce the area of column data paths since they require small areas.In addition,we secure device reliability by using HV(high-voltage)transistors in the WL driver.Furthermore,we change from a static logic to a dynamic logic used for the WL driver in the core circuit.Also,we optimize the WD(write data)switch circuit.Thus,we can implement them with a small-area design.In addition,we implement the address predecoder with a small-area logic circuit.The area of the designed 32 kbit OTP with 5V and HV devices is 674.725μm×258.75μm(=0.1745mm2)and is 56.3% smaller than that using 3.3V devices. 展开更多
关键词 one-time programmable memory micro controller unit EEPROM tunnel oxide small-area
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A One-Time Pad Encryption Scheme Based on Efficient Physical-Layer Secret Key Generation for Intelligent IoT System 被引量:1
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作者 Liquan Chen Kailin Cao +2 位作者 Tianyu Lu Yi Lu Aiqun Hu 《China Communications》 SCIE CSCD 2022年第7期185-196,共12页
The one-time pad(OTP)is an applicationlayer encryption technique to achieve the informationtheoretic security,and the physical-layer secret key generation(SKG)technique is a promising candidate to provide the random k... The one-time pad(OTP)is an applicationlayer encryption technique to achieve the informationtheoretic security,and the physical-layer secret key generation(SKG)technique is a promising candidate to provide the random keys for OTP.In this paper,we propose a joint SKG and OTP encryption scheme with the aid of a reconfigurable intelligent surface(RIS)to boost secret key rate.To maximize the efficiency of secure communication,we divide the process of secure transmission into two stages:SKG and then encrypted packet transmission.Meanwhile,we design an optimal algorithm for allocating time slots for SKG to maximize SKG efficiency without security risk.Furthermore,we design a key updating protocol based on our SKG scheme for OTP encryption.Simulation results verify that our scheme can generate keys securely and efficiently,and significantly improve the secure communication performance in an intelligent IoT system. 展开更多
关键词 physical layer security intelligent internet of things one-time pad reconfigurable intelligent surface
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One-time Shaping Technological System of Lightweight Roof Greening 被引量:1
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作者 殷金岩 许建新 +5 位作者 罗旭荣 骆华容 黎修东 唐巧玲 王玲玲 何新杰 《Agricultural Science & Technology》 CAS 2016年第6期1471-1474,共4页
Lightweight roof greening is an important way for improving urban ecological environment and has good ecological and social benefits, but the investment is- too-high for the investors. Therefore, it is necessary to im... Lightweight roof greening is an important way for improving urban ecological environment and has good ecological and social benefits, but the investment is- too-high for the investors. Therefore, it is necessary to improve the system of lightweight roof greening. This study introduced a lightweight roof greening mode with low cost, simple construction, rapid formation, good economic benefit and convenient curing. 展开更多
关键词 Lightweight roof greening one-time shaping technology Plant mat
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