Traumatic brain injury(TBI)remains a major public health challenge worldwide,particularly among individuals under45 years of age,resulting in profound physical disability,neuropsychiatric impairment,and socioeconomic ...Traumatic brain injury(TBI)remains a major public health challenge worldwide,particularly among individuals under45 years of age,resulting in profound physical disability,neuropsychiatric impairment,and socioeconomic burden.Existing therapeutic strategies offer limited efficacy,underscoring the urgent need for novel treatment modalities.This study evaluated the neuroprotective potential of OM-LV20,an amphibian-derived bioactive peptide,in a murine model of TBI.Intraperitoneal administration of OM-LV20 following controlled cortical impact significantly reduced brain water content and attenuated neurological and cognitive deficits.Histological analysis,TUNEL staining,and ultrastructural assessments revealed suppression of neuronal degeneration and mitigation of mitochondrial dysfunction.Transcriptomic profiling,together with RT-q PCR and western blot analyses,revealed modulation of the expression of apoptosis-related genes and proteins,indicating a robust anti-apoptotic effect mediated through mitochondrial pathways.Additionally,OM-LV20 exhibited efficient permeability across the blood-brain barrier.These findings indicate that OM-LV20 exerted strong neuroprotective activity through targeted suppression of mitochondriacentered neuronal apoptosis,supporting its potential utility in ameliorating neurological dysfunction post-TBI.The diverse mechanistic actions identified for OM-LV20 provide a novel therapeutic avenue with considerable promise for enhancing neurological recovery in individuals with TBI.展开更多
基金supported by the National Natural Science Foundation of China(82371468,32360138,81760648)Key Program of Yunnan Fundamental Research Project(202301AS070036)+4 种基金Outstanding Youth Program of Yunnan Applied Basic Research Project-Kunming Medical University Union Foundation(202301AY070001-301)First-Class Discipline Team of Skin&Mucosal Regenerative Medicine of Kunming Medical University(2024XKTDTS10)Yunnan Fundamental Research Projects(202201AT070295)Scientific Research Fund Project of Yunnan Provincial Department of Education(2023Y0609,2023Y0778)Kunming Medical University Talent Introduction Research Project(K132310505)。
文摘Traumatic brain injury(TBI)remains a major public health challenge worldwide,particularly among individuals under45 years of age,resulting in profound physical disability,neuropsychiatric impairment,and socioeconomic burden.Existing therapeutic strategies offer limited efficacy,underscoring the urgent need for novel treatment modalities.This study evaluated the neuroprotective potential of OM-LV20,an amphibian-derived bioactive peptide,in a murine model of TBI.Intraperitoneal administration of OM-LV20 following controlled cortical impact significantly reduced brain water content and attenuated neurological and cognitive deficits.Histological analysis,TUNEL staining,and ultrastructural assessments revealed suppression of neuronal degeneration and mitigation of mitochondrial dysfunction.Transcriptomic profiling,together with RT-q PCR and western blot analyses,revealed modulation of the expression of apoptosis-related genes and proteins,indicating a robust anti-apoptotic effect mediated through mitochondrial pathways.Additionally,OM-LV20 exhibited efficient permeability across the blood-brain barrier.These findings indicate that OM-LV20 exerted strong neuroprotective activity through targeted suppression of mitochondriacentered neuronal apoptosis,supporting its potential utility in ameliorating neurological dysfunction post-TBI.The diverse mechanistic actions identified for OM-LV20 provide a novel therapeutic avenue with considerable promise for enhancing neurological recovery in individuals with TBI.
