The ultimate goal of single-cell analyses is to obtain the biomolecular content for each cell in unicellular and multicellular organisms at different points of their life cycle under variable environmental conditions....The ultimate goal of single-cell analyses is to obtain the biomolecular content for each cell in unicellular and multicellular organisms at different points of their life cycle under variable environmental conditions.These require an assessment of:a)the total number of cells,b)the total number of cell types,and c)the complete and quantitative single molecular detection and identification for all classes of biopolymers,and organic and inorganic compounds,in each individual cell.For proteins,glycans,lipids,and metabolites,whose sequences cannot be amplified by copying as in the case of nucleic acids,the detection limit by mass spectrometry is about 105 molecules.Therefore,proteomic,glycomic,lipidomic,and metabolomic analyses do not yet permit the assembly of the complete single-cell omes.The construction of novel nanoelectrophoretic arrays and nano in microarrays on a single 1-cm-diameter chip has shown proof of concept for a high throughput platform for parallel processing of thousands of individual cells.Combined with dynamic secondary ion mass spectrometry,with 3D scanning capability and lateral resolution of 50 nm,the sensitivity of single molecular quantification and identification for all classes of biomolecules could be reached.Further development and routine application of such technological and instrumentation solution would allow assembly of complete omes with a quantitative assessment of structural and functional cellular diversity at the molecular level.展开更多
资源受限的移动设备因其有限的计算能力、存储资源、用户接口等,无法承载3G高带宽环境下丰富的移动网联网应用。通过借鉴云计算强大的计算和存储能力,提出一种"云+端"新型移动网联网应用研发模型:一方面,将复杂的逻辑计算和...资源受限的移动设备因其有限的计算能力、存储资源、用户接口等,无法承载3G高带宽环境下丰富的移动网联网应用。通过借鉴云计算强大的计算和存储能力,提出一种"云+端"新型移动网联网应用研发模型:一方面,将复杂的逻辑计算和庞大的存储量交给能力强大的云计算平台处理;另一方面,OMS(open mobile system)作为云计算的"端"平台,能够与云计算平台无缝整合,为用户提供真正意义上的、丰富的、高效的、廉价的移动互联网应用。同时,模型对于高带宽环境下新型移动通信软件研发具有较好的实践和推广价值。展开更多
文摘The ultimate goal of single-cell analyses is to obtain the biomolecular content for each cell in unicellular and multicellular organisms at different points of their life cycle under variable environmental conditions.These require an assessment of:a)the total number of cells,b)the total number of cell types,and c)the complete and quantitative single molecular detection and identification for all classes of biopolymers,and organic and inorganic compounds,in each individual cell.For proteins,glycans,lipids,and metabolites,whose sequences cannot be amplified by copying as in the case of nucleic acids,the detection limit by mass spectrometry is about 105 molecules.Therefore,proteomic,glycomic,lipidomic,and metabolomic analyses do not yet permit the assembly of the complete single-cell omes.The construction of novel nanoelectrophoretic arrays and nano in microarrays on a single 1-cm-diameter chip has shown proof of concept for a high throughput platform for parallel processing of thousands of individual cells.Combined with dynamic secondary ion mass spectrometry,with 3D scanning capability and lateral resolution of 50 nm,the sensitivity of single molecular quantification and identification for all classes of biomolecules could be reached.Further development and routine application of such technological and instrumentation solution would allow assembly of complete omes with a quantitative assessment of structural and functional cellular diversity at the molecular level.
基金supported by National Natural Science Foundation of China(11047030,11171055)Soft Science Program(112400430123)Basic and Forward Position Program of Henan Provincial Science and Technology Department(122300410385)
文摘资源受限的移动设备因其有限的计算能力、存储资源、用户接口等,无法承载3G高带宽环境下丰富的移动网联网应用。通过借鉴云计算强大的计算和存储能力,提出一种"云+端"新型移动网联网应用研发模型:一方面,将复杂的逻辑计算和庞大的存储量交给能力强大的云计算平台处理;另一方面,OMS(open mobile system)作为云计算的"端"平台,能够与云计算平台无缝整合,为用户提供真正意义上的、丰富的、高效的、廉价的移动互联网应用。同时,模型对于高带宽环境下新型移动通信软件研发具有较好的实践和推广价值。
