Abnormal accumulation of collagen fibrils is a hallmark feature of oral submucous fibrosis(OSF).However,the precise characteristics and underlying mechanisms remain unclear,impeding the advancement of potential therap...Abnormal accumulation of collagen fibrils is a hallmark feature of oral submucous fibrosis(OSF).However,the precise characteristics and underlying mechanisms remain unclear,impeding the advancement of potential therapeutic approaches.Here,we observed that collagen Ⅰ,the main component of the extracellular matrix,first accumulated in the lamina propria and subsequently in the submucosa of OSF specimens as the disease progressed.Using RNA-seq and Immunofluorescence in OSF specimens,we screened the cartilage oligomeric matrix protein(COMP)responsible for the abnormal collagen accumulation.Genetic COMP deficiency reduced arecoline-stimulated collagen I deposition significantly in vivo.In comparison,both COMP and collagen Ⅰ were upregulated under arecoline stimulation in wild-type mice.Human oral buccal mucosal fibroblasts(hBMFs)also exhibited increased secretion of COMP and collagen I after stimulation in vitro.COMP knockdown in hBMFs downregulates arecoline-stimulated collagen Ⅰ secretion.We further demonstrated that hBMFs present heterogeneous responses to arecoline stimulation,of which COMP-positive fibroblasts secrete more collagen Ⅰ.Since COMP is a molecular bridge with Fibril-associated collagens with Interrupted Triple helices(FACIT)in the collagen network,we further screened and identified collagen XIV,a FACIT member,co-localizing with both COMP and collagen Ⅰ.Collagen XIV expression increased under arecoline stimulation in wild-type mice,whereas it was hardly expressed in the Comp^(-/-) mice,even with under stimulation.In summary,we found that COMP may mediates abnormal collagen Ⅰ deposition by functions with collagen XIV during the progression of OSF,suggesting its potential to be targeted in treating OSF.展开更多
利用遥感技术快速准确地提取耕地信息是耕地保护的关键环节。以山东省商河县为例,提出了一种基于多季相分形特征的Landsat 8 OLI影像耕地信息提取方法。首先采用毯子覆盖法计算多季相遥感影像每个像元的上分形信号和下分形信号,对比分...利用遥感技术快速准确地提取耕地信息是耕地保护的关键环节。以山东省商河县为例,提出了一种基于多季相分形特征的Landsat 8 OLI影像耕地信息提取方法。首先采用毯子覆盖法计算多季相遥感影像每个像元的上分形信号和下分形信号,对比分析耕地和其他土地利用类型的分形特征,选取上分形信号的第3尺度作为特征尺度,提取商河县耕地空间分布特征;其次采用同时期的土地利用矢量数据、Esri land cover数据和统计数据进行耕地信息提取精度评价;最后分别设置多季相分形提取与单季相分形提取、现有土地利用数据产品的对比实验,并基于点位匹配度和面积匹配度进行评价。结果表明:多季相数据更能反映农作物生长的复杂性,有助于提高耕地信息的提取精度;不同土地利用类型在不同分形尺度的信号值各不相同,分形特征可以在不同尺度上清晰地刻画出不同土地利用类型的分异性;基于矢量数据和Esri land cover数据评价的多季相分形特征耕地提取点位匹配度为87.13%和89.83%,面积匹配度为99.73%和97.91%,均比单季相分形提取结果精度高;综合考虑点位匹配度、面积匹配度和空间分布特征,研发方法能有效区分耕地和其他土地利用类型,提取结果更优,且与统计数据有更高的一致性。该方法可准确提取耕地信息,为耕地的动态监测和损害评估提供技术支撑。展开更多
The integration of remote sensing and geographic information system(GIS)was employed in this study to delineate the structural lineaments within the eastern section of the Ouarzazate Basin,situated between the souther...The integration of remote sensing and geographic information system(GIS)was employed in this study to delineate the structural lineaments within the eastern section of the Ouarzazate Basin,situated between the southern front of the Central High Atlas and the northern slopes of the Eastern Anti-Atlas(also known as the Saghro Massif).To achieve this objective,Landsat 8 Operational Land Imager(OLI)and Shuttle Radar Topography Mission(SRTM)data were used.Principal Component Analysis(PCA)was computed and a directional filter was applied to the first PCA and the panchromatic band(B8).Additionally,shading was applied to the SRTM data in four directions;N0°,N45°,N90°,N135°.After removing of the non-geological linear structures,the results obtained,using the automatic extraction method,allowed us to produce a synthetic map that included 1251 lineaments with an average length of 1331 m and was dominated by NE-SW,ENE-WSW and E-W directions,respectively.However,the high lineament density is clearly noted in the Anti-Atlas(Saghro Massif)and at the level of the northern part,extending from the Ait Ibrirne to Arg-Ali Oubourk villages.High lineament density can always be found around the major faults affecting this area.The data collected during the field investigations and from geological maps show that the major direction of the faults and structural accidents range mostly between N45°,N70°and N75°.The correlation of remote sensing results with those collected in the field shows a similarity and coincidence with each other.From these results,it is possible to consider the automatic extraction method as a supplementary kind that can serve classical geology by quickly enriching it with additional data.As shown in this work,this method provides more information when applied in arid areas where the fields are well outcropped.展开更多
基金supported by the National Natural Science Foundation of China grant(81974150).
文摘Abnormal accumulation of collagen fibrils is a hallmark feature of oral submucous fibrosis(OSF).However,the precise characteristics and underlying mechanisms remain unclear,impeding the advancement of potential therapeutic approaches.Here,we observed that collagen Ⅰ,the main component of the extracellular matrix,first accumulated in the lamina propria and subsequently in the submucosa of OSF specimens as the disease progressed.Using RNA-seq and Immunofluorescence in OSF specimens,we screened the cartilage oligomeric matrix protein(COMP)responsible for the abnormal collagen accumulation.Genetic COMP deficiency reduced arecoline-stimulated collagen I deposition significantly in vivo.In comparison,both COMP and collagen Ⅰ were upregulated under arecoline stimulation in wild-type mice.Human oral buccal mucosal fibroblasts(hBMFs)also exhibited increased secretion of COMP and collagen I after stimulation in vitro.COMP knockdown in hBMFs downregulates arecoline-stimulated collagen Ⅰ secretion.We further demonstrated that hBMFs present heterogeneous responses to arecoline stimulation,of which COMP-positive fibroblasts secrete more collagen Ⅰ.Since COMP is a molecular bridge with Fibril-associated collagens with Interrupted Triple helices(FACIT)in the collagen network,we further screened and identified collagen XIV,a FACIT member,co-localizing with both COMP and collagen Ⅰ.Collagen XIV expression increased under arecoline stimulation in wild-type mice,whereas it was hardly expressed in the Comp^(-/-) mice,even with under stimulation.In summary,we found that COMP may mediates abnormal collagen Ⅰ deposition by functions with collagen XIV during the progression of OSF,suggesting its potential to be targeted in treating OSF.
文摘利用遥感技术快速准确地提取耕地信息是耕地保护的关键环节。以山东省商河县为例,提出了一种基于多季相分形特征的Landsat 8 OLI影像耕地信息提取方法。首先采用毯子覆盖法计算多季相遥感影像每个像元的上分形信号和下分形信号,对比分析耕地和其他土地利用类型的分形特征,选取上分形信号的第3尺度作为特征尺度,提取商河县耕地空间分布特征;其次采用同时期的土地利用矢量数据、Esri land cover数据和统计数据进行耕地信息提取精度评价;最后分别设置多季相分形提取与单季相分形提取、现有土地利用数据产品的对比实验,并基于点位匹配度和面积匹配度进行评价。结果表明:多季相数据更能反映农作物生长的复杂性,有助于提高耕地信息的提取精度;不同土地利用类型在不同分形尺度的信号值各不相同,分形特征可以在不同尺度上清晰地刻画出不同土地利用类型的分异性;基于矢量数据和Esri land cover数据评价的多季相分形特征耕地提取点位匹配度为87.13%和89.83%,面积匹配度为99.73%和97.91%,均比单季相分形提取结果精度高;综合考虑点位匹配度、面积匹配度和空间分布特征,研发方法能有效区分耕地和其他土地利用类型,提取结果更优,且与统计数据有更高的一致性。该方法可准确提取耕地信息,为耕地的动态监测和损害评估提供技术支撑。
文摘The integration of remote sensing and geographic information system(GIS)was employed in this study to delineate the structural lineaments within the eastern section of the Ouarzazate Basin,situated between the southern front of the Central High Atlas and the northern slopes of the Eastern Anti-Atlas(also known as the Saghro Massif).To achieve this objective,Landsat 8 Operational Land Imager(OLI)and Shuttle Radar Topography Mission(SRTM)data were used.Principal Component Analysis(PCA)was computed and a directional filter was applied to the first PCA and the panchromatic band(B8).Additionally,shading was applied to the SRTM data in four directions;N0°,N45°,N90°,N135°.After removing of the non-geological linear structures,the results obtained,using the automatic extraction method,allowed us to produce a synthetic map that included 1251 lineaments with an average length of 1331 m and was dominated by NE-SW,ENE-WSW and E-W directions,respectively.However,the high lineament density is clearly noted in the Anti-Atlas(Saghro Massif)and at the level of the northern part,extending from the Ait Ibrirne to Arg-Ali Oubourk villages.High lineament density can always be found around the major faults affecting this area.The data collected during the field investigations and from geological maps show that the major direction of the faults and structural accidents range mostly between N45°,N70°and N75°.The correlation of remote sensing results with those collected in the field shows a similarity and coincidence with each other.From these results,it is possible to consider the automatic extraction method as a supplementary kind that can serve classical geology by quickly enriching it with additional data.As shown in this work,this method provides more information when applied in arid areas where the fields are well outcropped.
