Cytochrome P450 OleT_(SA),a new cytochrome P450 enzyme from Staphylococcus aureus,catalyzes the oxidative decarboxylation and hydroxylation of fatty acids to generate terminal alkenes and fatty alcohols.The mechanism ...Cytochrome P450 OleT_(SA),a new cytochrome P450 enzyme from Staphylococcus aureus,catalyzes the oxidative decarboxylation and hydroxylation of fatty acids to generate terminal alkenes and fatty alcohols.The mechanism of this bifurcative chemistry remains largely unknown.Herein,a class of derivatized fatty acids were synthesized as probes to investigate the effects of substrate structure on the product type of P450 OleT_(SA).The results demonstrate that the fine-tuned structure of substrates,even in a remote distance from the carboxyl group,significantly regulates OleT catalyzed decarboxylation/hydroxylation reactions.Molecular docking analysis indicated the potential interactions between the carboxylate groups of different probes and the enzyme active center which was attributed to the bifurcative chemistry.展开更多
Key challenges in the development of organic light-emitting transistors(OLETs)are blocking both scientific research and practical applications of these devices,e.g.,the absence of high-mobility emissive organic semico...Key challenges in the development of organic light-emitting transistors(OLETs)are blocking both scientific research and practical applications of these devices,e.g.,the absence of high-mobility emissive organic semiconductor materials,low device efficiency,and color tunability.Here,we report a novel device configuration called the energy transfer organic light-emitting transistor(ET-OLET)that is intended to overcome these challenges.An organic fluorescent dye-doped polymethyl methacrylate(PMMA)layer is inserted below the conventional high-mobility organic semiconductor layer in a single-component OLET to separate the functions of the charge transport and light-emitting layers,thus making the challenge to essentially integrate the high mobility and emissive functions within a single organic semiconductor in a conventional OLET or multilayer OLET unnecessary.In this architecture,there is little change in mobility,but the external quantum efficiency(EQE)of the ET-OLET is more than six times that of the conventional OLET because of the efficient Förster resonance energy transfer,which avoids exciton-charge annihilation.In addition,the emission color can be tuned from blue to white to green-yellow using the sourcedrain and gate voltages.The proposed structure is promising for use with electrically pumped organic lasers.展开更多
基金the support from the State Scholarship Fund of the China Scholarship Council(No.201806310084)。
文摘Cytochrome P450 OleT_(SA),a new cytochrome P450 enzyme from Staphylococcus aureus,catalyzes the oxidative decarboxylation and hydroxylation of fatty acids to generate terminal alkenes and fatty alcohols.The mechanism of this bifurcative chemistry remains largely unknown.Herein,a class of derivatized fatty acids were synthesized as probes to investigate the effects of substrate structure on the product type of P450 OleT_(SA).The results demonstrate that the fine-tuned structure of substrates,even in a remote distance from the carboxyl group,significantly regulates OleT catalyzed decarboxylation/hydroxylation reactions.Molecular docking analysis indicated the potential interactions between the carboxylate groups of different probes and the enzyme active center which was attributed to the bifurcative chemistry.
基金This work was supported financially by the National Natural Science Foundation of China(Nos.51602200,61874074,51633006,51703160,91433115,21473222,and 21661132006)the Key Project of the Department of Education of Guangdong Province(No.2016KZDXM008)+1 种基金the Shenzhen Peacock Plan(No.KQTD2016053112042971)the Chinese Academy of Sciences.
文摘Key challenges in the development of organic light-emitting transistors(OLETs)are blocking both scientific research and practical applications of these devices,e.g.,the absence of high-mobility emissive organic semiconductor materials,low device efficiency,and color tunability.Here,we report a novel device configuration called the energy transfer organic light-emitting transistor(ET-OLET)that is intended to overcome these challenges.An organic fluorescent dye-doped polymethyl methacrylate(PMMA)layer is inserted below the conventional high-mobility organic semiconductor layer in a single-component OLET to separate the functions of the charge transport and light-emitting layers,thus making the challenge to essentially integrate the high mobility and emissive functions within a single organic semiconductor in a conventional OLET or multilayer OLET unnecessary.In this architecture,there is little change in mobility,but the external quantum efficiency(EQE)of the ET-OLET is more than six times that of the conventional OLET because of the efficient Förster resonance energy transfer,which avoids exciton-charge annihilation.In addition,the emission color can be tuned from blue to white to green-yellow using the sourcedrain and gate voltages.The proposed structure is promising for use with electrically pumped organic lasers.
