Octacosanol is purified by agitated short-path distillation (SPD). Effects of evaporation temperature,number of SPD steps in series and other distillation method on the octacosanol recovery and decomposition are studi...Octacosanol is purified by agitated short-path distillation (SPD). Effects of evaporation temperature,number of SPD steps in series and other distillation method on the octacosanol recovery and decomposition are studied. Although the experimental results indicate some decomposition when the mixture of higher primary aliphatic alcohols is distillated by SPD, SPD is still an effective method to purify octacosanol. It is concluded that evaporation temperature affects greatly on the purity and recovery of octacosanol.展开更多
该研究采用亚临界水萃取法(Subcritical Water Extraction,SWE)提取滤泥中二十八烷醇,通过单因素试验及响应面分析优化提取工艺,并对其成分进行分析,然后对比传统热乙醇回流提取法研究其提取效果。结果表明,亚临界水萃取滤泥的最佳工艺...该研究采用亚临界水萃取法(Subcritical Water Extraction,SWE)提取滤泥中二十八烷醇,通过单因素试验及响应面分析优化提取工艺,并对其成分进行分析,然后对比传统热乙醇回流提取法研究其提取效果。结果表明,亚临界水萃取滤泥的最佳工艺条件为:滤泥添加量250 g,萃取时间65 min,萃取温度180℃,萃取压力4.5 MPa。在此条件下,亚临界水萃取二十八烷醇的萃取率达29.77%,纯度达70.89%,明显优于热乙醇回流提取法;通过GC-MS技术和红外光谱检测到所得样品除含二十八烷醇外,还含有二十一烷醇,二十六烷醇,二十七烷醇、三十烷醇、菜油甾醇和七聚乙二醇等其他高级脂肪醇,具有抗氧化活性、降胆固醇等功效,均对人体有益。这表明亚临界水萃取法制备二十八烷醇具有高提取率、高纯度、绿色环保的优点,为糖厂滤泥中二十八烷醇的高值化利用提供一定的参考和理论基础。展开更多
The object of this study was to explore the regulatory mechanism of octacosanol to the body of animals and the effects of octacosanol on blood hormone levels and gene expressions of glucose transporter protein（GLUT-4...The object of this study was to explore the regulatory mechanism of octacosanol to the body of animals and the effects of octacosanol on blood hormone levels and gene expressions of glucose transporter protein（GLUT-4） and adenosine monophosphate protein kinase（AMPK） in liver and muscle tissue of weaning piglets. A total of 105 crossbred piglets（[Yorkshire x Landrace] x Duroc） with an initial BW of5.70 ± 1.41 kg（21 d of age） were used in a 6-wk trial to evaluate the effects of octacosanol and tiamulin supplementation on contents of triiodothyronine（T3）,thyroxine（T4）,growth hormone（GH）,glucagon（GU） and adrenaline（AD） in blood and gene expressions of GLUT-4 and AMPK in liver and muscle. Piglets were randomly distributed into 3 dietary treatments on the basis of BW and sex. Each treatment had 7 replicate pens with 5 piglets per pen. Treatments were as followed: control group, tiamulin group and octacosanol group. The results showed that compared with control group and tiamulin group, octacosanol greatly promoted the secretion of T3, GH, GU and AD（P < 0.01） and significantly up-regulated the gene expressions of GLUT-4 and AMPK in muscle and liver tissues（P < 0.05）. There was no significant difference between the control group and tiamulin group in T3, T4, GH,GU or AD（P> 0.05）, Results of the present study has confirmed that octacosanol affects energy metabolism of body by regulating secretion of blood hormones and related gene expression in tissue of weaning piglets, which can reduce stress response and has an impact on performance.展开更多
Octacosanol is a widely distributed natural higher aliphatic alcohol that can be isolated and purified from rice bran,sugarcane,beeswax,insect wax,etc.Octacosanol exerts various biological effects,including anti-fatig...Octacosanol is a widely distributed natural higher aliphatic alcohol that can be isolated and purified from rice bran,sugarcane,beeswax,insect wax,etc.Octacosanol exerts various biological effects,including anti-fatigue,anti-hypoxia,antioxidant,anti-inflammatory,antitumor,etc.Meanwhile,it has the effects of regulating the body's immune function and energy metabolism and has potential benefits for cardiovascular disease,cerebrovascular disorders,diabetes,Parkinson's disease,and others.Octacosanol is primarily responsible for regulating multiple signaling pathways,such as AMPK,PI3K/Akt,and MAPK/NF-κB,to achieve different physiological functions.This review systemically summarized the progress in characterization,extraction and purification,biological functions,molecular mechanisms,and bioavailability of octacosanol.This study will provide a reference for many investigators to further explore the physiological functions of higher aliphatic alcohols and apply them as supplements in functional foods.展开更多
Octacosanol in blood was extracted with dichloricmethane.It was derivatized to trimethylsilyl ethers by N,O-Bis(trimethylsilyl) trifluoroacetamide.The compound was determined by gas chromatography-mass spectrometry an...Octacosanol in blood was extracted with dichloricmethane.It was derivatized to trimethylsilyl ethers by N,O-Bis(trimethylsilyl) trifluoroacetamide.The compound was determined by gas chromatography-mass spectrometry and selected ion monitoring(SIM).Quantitative analysis was performed by measuring the area and comparing it to a calibration curve.展开更多
基金Supported by the National Natural Science Foundation of China(No. 20176037), and the Development Foundation from the Science & Technology Commission of Tianjin, China (No. 01310864).
文摘Octacosanol is purified by agitated short-path distillation (SPD). Effects of evaporation temperature,number of SPD steps in series and other distillation method on the octacosanol recovery and decomposition are studied. Although the experimental results indicate some decomposition when the mixture of higher primary aliphatic alcohols is distillated by SPD, SPD is still an effective method to purify octacosanol. It is concluded that evaporation temperature affects greatly on the purity and recovery of octacosanol.
基金provided by China Agriculture Research System-Beijing Team for Poultry Industry(CARS-PSTP)the National Science&Technology Pillar Program(2011BAD26B04,Beijing,China)
文摘The object of this study was to explore the regulatory mechanism of octacosanol to the body of animals and the effects of octacosanol on blood hormone levels and gene expressions of glucose transporter protein（GLUT-4） and adenosine monophosphate protein kinase（AMPK） in liver and muscle tissue of weaning piglets. A total of 105 crossbred piglets（[Yorkshire x Landrace] x Duroc） with an initial BW of5.70 ± 1.41 kg（21 d of age） were used in a 6-wk trial to evaluate the effects of octacosanol and tiamulin supplementation on contents of triiodothyronine（T3）,thyroxine（T4）,growth hormone（GH）,glucagon（GU） and adrenaline（AD） in blood and gene expressions of GLUT-4 and AMPK in liver and muscle. Piglets were randomly distributed into 3 dietary treatments on the basis of BW and sex. Each treatment had 7 replicate pens with 5 piglets per pen. Treatments were as followed: control group, tiamulin group and octacosanol group. The results showed that compared with control group and tiamulin group, octacosanol greatly promoted the secretion of T3, GH, GU and AD（P < 0.01） and significantly up-regulated the gene expressions of GLUT-4 and AMPK in muscle and liver tissues（P < 0.05）. There was no significant difference between the control group and tiamulin group in T3, T4, GH,GU or AD（P> 0.05）, Results of the present study has confirmed that octacosanol affects energy metabolism of body by regulating secretion of blood hormones and related gene expression in tissue of weaning piglets, which can reduce stress response and has an impact on performance.
基金supported by the[National Natural Science Foundation of China]under Grant[number 31571874][the Grain-oil Process and Quality Control 2011 Collaborative and Innovative Grant from Hunan Province]under Grant[2013,number 448]+1 种基金[the Key Project of the Education Department of Hunan Province]under Grant[number 16A228,13A124][the Graduate Innovative Research Project of Hunan province and Central South University of Forestry and Technology]under Grant[number CX20200699,CX20201018].
文摘Octacosanol is a widely distributed natural higher aliphatic alcohol that can be isolated and purified from rice bran,sugarcane,beeswax,insect wax,etc.Octacosanol exerts various biological effects,including anti-fatigue,anti-hypoxia,antioxidant,anti-inflammatory,antitumor,etc.Meanwhile,it has the effects of regulating the body's immune function and energy metabolism and has potential benefits for cardiovascular disease,cerebrovascular disorders,diabetes,Parkinson's disease,and others.Octacosanol is primarily responsible for regulating multiple signaling pathways,such as AMPK,PI3K/Akt,and MAPK/NF-κB,to achieve different physiological functions.This review systemically summarized the progress in characterization,extraction and purification,biological functions,molecular mechanisms,and bioavailability of octacosanol.This study will provide a reference for many investigators to further explore the physiological functions of higher aliphatic alcohols and apply them as supplements in functional foods.
文摘Octacosanol in blood was extracted with dichloricmethane.It was derivatized to trimethylsilyl ethers by N,O-Bis(trimethylsilyl) trifluoroacetamide.The compound was determined by gas chromatography-mass spectrometry and selected ion monitoring(SIM).Quantitative analysis was performed by measuring the area and comparing it to a calibration curve.
