Objective To determine the effects of genetic variation in the organic cation transporter 1(OCT1)on the short-term responses of the antidiabetic drug,metformin.Method A total of 22 patients recruited with type 2 diabe...Objective To determine the effects of genetic variation in the organic cation transporter 1(OCT1)on the short-term responses of the antidiabetic drug,metformin.Method A total of 22 patients recruited with type 2 diabetes or IFG were treated with metformin(2 000 mg/day)for 1 week.The patients were screened from Second Jikun hospital and Kashidonglu community medicine service,Urumqi,China and their surrounding districts.To examine the effects of metformin on plasma glucose,total cholesterol,low-density lipoprotein-cholesterol,high-density lipoprotein-cholesterol and triglyceride in relation with R61C,G465R and 420 del variants of OCT1(gene encoding organic cation transporter 1,mainly locating in liver,which is metformin's major target)in subjects.In all,R61C,G465R and 420del of OCT1 gene were examined using DNA extracted from whole blood and PCR-RFLP.Data concerning with gene and metformin treatment were handled by t-test.Result After metformin treatment,there were increases both in FPG and LDL(P=0.011and P=0.013 respectively).To divide all participants into mutant and wild groups,according to the polymorphisms of R61C,G465R and 420 del respectively,as well as carriers with one of the mutant genotypes at least and carriers with none of the mutant sites.Analysis was made to compared FPG,Chol,TG,and LDL and HDL between carriers of wild genotypes and carriers of other genotypes showed no statistic significance both before the metformin treatment and after the treatment.The same is the case with changes of FPG,Chol,TG,and LDL and HDL of wild genotype carriers and variant genotype carriers,except of LDL changes(P=0.05)in patients grouped by G465R polymorphisms and TG changes(P=0.03)in subjects differed by 420del genotypes.Conclusion In this study,it is suggested that OCT1 gene polymorphisms have little contribution to the clinical efficacy of blood glucose control by metformin among Uygur people with type 2 diabetes or IFG,but it may have possible relationship with the clinical efficacy on fat metabolism by metformin.展开更多
目的探讨有机阳离子转运蛋白1(OCT1)(rs622342)和多药及毒素外排转运蛋白1(MATE1)(rs2289669)基因多态性对接受二甲双胍治疗的2型糖尿病患者血糖控制指标的影响。方法采用横断面研究方法,所有确诊为2型糖尿病的参与者接受盐酸二甲双胍片...目的探讨有机阳离子转运蛋白1(OCT1)(rs622342)和多药及毒素外排转运蛋白1(MATE1)(rs2289669)基因多态性对接受二甲双胍治疗的2型糖尿病患者血糖控制指标的影响。方法采用横断面研究方法,所有确诊为2型糖尿病的参与者接受盐酸二甲双胍片1~2 g/d治疗至少3个月,记录血糖控制水平、代谢指标,并测定OCT1和MATE1基因型,使用IBM SPSS Statistics for Windows V22.0统计分析不同基因型与患者血糖控制水平、代谢指标之间的相关性。结果共纳入191名2型糖尿病的患者。MATE1(rs2289669)AA/AG基因型携带者的空腹血糖(FPG)水平显著低于GG基因型患者,高密度脂蛋白胆固醇(HDL-C)水平比GG型显著升高。Logistics回归结果发现,MATE1(rs2289669)对于FPG、身体质量指数(BMI)和HDL-C有统计学意义。交互作用模型分析发现,在FPG中,同时拥有OCT1(rs622342)AA型和MATE1(rs228966)GG型基因的患者具有明显的交互作用且为协同作用,无明显相乘作用。结论OCT1和MATE1基因多态性与接受二甲双胍治疗的2型糖尿病患者血糖控制有显著关联,在与FPG相关性方面存在协同效应,也与脂代谢和BMI有关。OCT1和MATE1基因多态性可能在二甲双胍治疗2型糖尿病患者血糖控制和代谢指标方面具有预测作用。展开更多
Overexpression of receptor-interacting protein 140（RIP140） promotes neuronal differentiation of N2 a cells via extracellular regulated kinase 1/2（ERK1/2） signaling.However,involvement of RIP140 in human neural dif...Overexpression of receptor-interacting protein 140（RIP140） promotes neuronal differentiation of N2 a cells via extracellular regulated kinase 1/2（ERK1/2） signaling.However,involvement of RIP140 in human neural differentiation remains unclear.We found both RIP140 and ERK1/2 expression increased during neural differentiation of H1 human embryonic stem cells.Moreover,RIP140 negatively correlated with stem cell markers Oct4 and Sox2 during early stages of neural differentiation,and positively correlated with the neural stem cell marker Nestin during later stages.Thus,ERK1/2 signaling may provide the molecular mechanism by which RIP140 takes part in neural differentiation to eventually affect the number of neurons produced.展开更多
Date Oct.29th-Nov.Ist,2007 Venue Shanghai New International Expo Centre 2345 Long Yang Road Pudong Area Shanghai 201204 China Tel(86)21 2890 6666 Fax(86)21 6856 6777 Exhibition Space 80,500 sqm gross(2006)
基金The National Natural Science Foundation of China(30760288)Xinjiang Uygur Autonomic Tackle Key Problems Plans(200633129)Science and Technology Program of Urumqi(G07231001)
文摘Objective To determine the effects of genetic variation in the organic cation transporter 1(OCT1)on the short-term responses of the antidiabetic drug,metformin.Method A total of 22 patients recruited with type 2 diabetes or IFG were treated with metformin(2 000 mg/day)for 1 week.The patients were screened from Second Jikun hospital and Kashidonglu community medicine service,Urumqi,China and their surrounding districts.To examine the effects of metformin on plasma glucose,total cholesterol,low-density lipoprotein-cholesterol,high-density lipoprotein-cholesterol and triglyceride in relation with R61C,G465R and 420 del variants of OCT1(gene encoding organic cation transporter 1,mainly locating in liver,which is metformin's major target)in subjects.In all,R61C,G465R and 420del of OCT1 gene were examined using DNA extracted from whole blood and PCR-RFLP.Data concerning with gene and metformin treatment were handled by t-test.Result After metformin treatment,there were increases both in FPG and LDL(P=0.011and P=0.013 respectively).To divide all participants into mutant and wild groups,according to the polymorphisms of R61C,G465R and 420 del respectively,as well as carriers with one of the mutant genotypes at least and carriers with none of the mutant sites.Analysis was made to compared FPG,Chol,TG,and LDL and HDL between carriers of wild genotypes and carriers of other genotypes showed no statistic significance both before the metformin treatment and after the treatment.The same is the case with changes of FPG,Chol,TG,and LDL and HDL of wild genotype carriers and variant genotype carriers,except of LDL changes(P=0.05)in patients grouped by G465R polymorphisms and TG changes(P=0.03)in subjects differed by 420del genotypes.Conclusion In this study,it is suggested that OCT1 gene polymorphisms have little contribution to the clinical efficacy of blood glucose control by metformin among Uygur people with type 2 diabetes or IFG,but it may have possible relationship with the clinical efficacy on fat metabolism by metformin.
文摘目的探讨有机阳离子转运蛋白1(OCT1)(rs622342)和多药及毒素外排转运蛋白1(MATE1)(rs2289669)基因多态性对接受二甲双胍治疗的2型糖尿病患者血糖控制指标的影响。方法采用横断面研究方法,所有确诊为2型糖尿病的参与者接受盐酸二甲双胍片1~2 g/d治疗至少3个月,记录血糖控制水平、代谢指标,并测定OCT1和MATE1基因型,使用IBM SPSS Statistics for Windows V22.0统计分析不同基因型与患者血糖控制水平、代谢指标之间的相关性。结果共纳入191名2型糖尿病的患者。MATE1(rs2289669)AA/AG基因型携带者的空腹血糖(FPG)水平显著低于GG基因型患者,高密度脂蛋白胆固醇(HDL-C)水平比GG型显著升高。Logistics回归结果发现,MATE1(rs2289669)对于FPG、身体质量指数(BMI)和HDL-C有统计学意义。交互作用模型分析发现,在FPG中,同时拥有OCT1(rs622342)AA型和MATE1(rs228966)GG型基因的患者具有明显的交互作用且为协同作用,无明显相乘作用。结论OCT1和MATE1基因多态性与接受二甲双胍治疗的2型糖尿病患者血糖控制有显著关联,在与FPG相关性方面存在协同效应,也与脂代谢和BMI有关。OCT1和MATE1基因多态性可能在二甲双胍治疗2型糖尿病患者血糖控制和代谢指标方面具有预测作用。
基金supported by the National Natural Science Foundation of China,No.31340024
文摘Overexpression of receptor-interacting protein 140（RIP140） promotes neuronal differentiation of N2 a cells via extracellular regulated kinase 1/2（ERK1/2） signaling.However,involvement of RIP140 in human neural differentiation remains unclear.We found both RIP140 and ERK1/2 expression increased during neural differentiation of H1 human embryonic stem cells.Moreover,RIP140 negatively correlated with stem cell markers Oct4 and Sox2 during early stages of neural differentiation,and positively correlated with the neural stem cell marker Nestin during later stages.Thus,ERK1/2 signaling may provide the molecular mechanism by which RIP140 takes part in neural differentiation to eventually affect the number of neurons produced.
文摘Date Oct.29th-Nov.Ist,2007 Venue Shanghai New International Expo Centre 2345 Long Yang Road Pudong Area Shanghai 201204 China Tel(86)21 2890 6666 Fax(86)21 6856 6777 Exhibition Space 80,500 sqm gross(2006)
