Objective： To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. Methods： The influences of cisplatin （2.5 μg/mL） and/or cordycapin tr...Objective： To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. Methods： The influences of cisplatin （2.5 μg/mL） and/or cordycapin treatment （10 or 100 μmol/L） to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium （MTT） assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Results： Data demonstrated that co-administration of cisplatin （2.5 μg/mL） and cordycepin （10 or 100μmol/L） resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycapin alone treatment （24 h）, respectively （P〈0.05）. In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin （30%） was significantly higher than cisplatin （5%） or cordycepin （12%） alone group （P〈0.05）, confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/dun terminal kinase （JNK）, the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase （PARP） as compared to cisplatin or cordycepin alone treatment （P〈0.05）. Conclusion： Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.展开更多
Methoxybenzaldehyde is prepared from the addition-hydrolysis reaction of p-CH 3OC 6H 4MgBr with 1,3-disubstituted benzimidazolium salt. The reaction mechanism is discussed. 1,3-Dialkyl has little influence on the yield.
By using self-made metal-alkoxide yttrium isopropoxide as a sintering additive and disperser of whisker, the SiC whisker reinforced AIN ceramics was prepared. Its apparent density is 99.5 percent of the theoretical de...By using self-made metal-alkoxide yttrium isopropoxide as a sintering additive and disperser of whisker, the SiC whisker reinforced AIN ceramics was prepared. Its apparent density is 99.5 percent of the theoretical density; its flexural strength and fracture toughness are 681 MPa and 5.21 MPa·m1/2 respectively. Comparing the result with that by applying Y2O3 powder as a sintering additive, the flexural strength is increased by 25% and the fracture toughness is increased by 33% . The dispersity of whisker by increased yttrium isopropoxide is significantly better than that by Triton X-100.展开更多
基金Supported by National Science Council Grant(No.982320-B-006-016),Taiwan,China
文摘Objective： To evaluate apoptotic effects of cisplatin and cordycepin as single agent or in combination with cytotoxicity in oral cancer cells. Methods： The influences of cisplatin （2.5 μg/mL） and/or cordycapin treatment （10 or 100 μmol/L） to human OC3 oral cancer cell line were investigated by morphological observation for cell death appearance, methylthiazoletetrazolium （MTT） assay for cell viability, flow cytometry assay for cell apoptosis, and Western blotting for apoptotic protein expressions. Results： Data demonstrated that co-administration of cisplatin （2.5 μg/mL） and cordycepin （10 or 100μmol/L） resulted in the enhancement of OC3 cell apoptosis compared to cisplatin or cordycapin alone treatment （24 h）, respectively （P〈0.05）. In flow cytometry assay, percentage of cells arrested at subG1 phase with co-treatment of cordycepin and cisplatin （30%） was significantly higher than cisplatin （5%） or cordycepin （12%） alone group （P〈0.05）, confirming a synergistically apoptotic effect of cordycepin and cisplatin. In cellular mechanism study, co-treatment of cordycepin and cisplatin induced more stress-activated protein kinase/dun terminal kinase （JNK）, the expressions of caspase-7, and the cleavage of poly ADP-ribose polymerase （PARP） as compared to cisplatin or cordycepin alone treatment （P〈0.05）. Conclusion： Cisplatin and cordycepin possess synergistically apoptotic effect through the activation of JNK/caspase-7/PARP pathway in human OC3 oral cancer cell line.
文摘Methoxybenzaldehyde is prepared from the addition-hydrolysis reaction of p-CH 3OC 6H 4MgBr with 1,3-disubstituted benzimidazolium salt. The reaction mechanism is discussed. 1,3-Dialkyl has little influence on the yield.
文摘By using self-made metal-alkoxide yttrium isopropoxide as a sintering additive and disperser of whisker, the SiC whisker reinforced AIN ceramics was prepared. Its apparent density is 99.5 percent of the theoretical density; its flexural strength and fracture toughness are 681 MPa and 5.21 MPa·m1/2 respectively. Comparing the result with that by applying Y2O3 powder as a sintering additive, the flexural strength is increased by 25% and the fracture toughness is increased by 33% . The dispersity of whisker by increased yttrium isopropoxide is significantly better than that by Triton X-100.
