采用Illumina MiSeq高通量测序技术,对臭氧-生物活性炭水处理工艺过程各单元出水中细菌多样性及丰度进行研究,测序获得196 809条16S r DNA基因序列,归类为38个门,522个属。各样品中细菌多样性分析结果表明:在各处理单元出水中细菌群落...采用Illumina MiSeq高通量测序技术,对臭氧-生物活性炭水处理工艺过程各单元出水中细菌多样性及丰度进行研究,测序获得196 809条16S r DNA基因序列,归类为38个门,522个属。各样品中细菌多样性分析结果表明:在各处理单元出水中细菌群落具有高度多样性,预臭氧和臭氧氧化处理对水体中细菌多样性及丰度的影响最大,可杀灭一部分属的细菌,可合理控制臭氧浓度杀灭部分耐氯菌;絮凝沉淀和沙滤单元处理对水体中细菌多样性具有恢复效果,使水体中细菌种属进一步增多;在生物活性炭滤池处理后,细菌多样性增加,丰度分布更为均匀,对后期消毒工艺提出了更高的要求。展开更多
Simulated drinking water distribution system(DWDS) treated with O_3-BAC-Cl_2(ozonebiological activated carbon-chlorine) was constructed to study its effects on the regrowth of five typical opportunistic pathogens(OPs)...Simulated drinking water distribution system(DWDS) treated with O_3-BAC-Cl_2(ozonebiological activated carbon-chlorine) was constructed to study its effects on the regrowth of five typical opportunistic pathogens(OPs). It was found that O_3-BAC-Cl_2 could significantly reduce the regrowth of target OPs in the effluents of DWDS compared with Cl_2 and O_3-Cl_2 with the same residual chlorine levels. However, the effect of O_3-BAC-Cl_2 on the average numbers of target OPs gene markers in the biofilms of DWDS was not apparent, suggesting that OPs in the biofilms of DWDS were tolerant to the upstream disinfection process. The quantification of target OPs in the BAC-filter column demonstrated that OPs decreased with the increase of depth, which was likely due to the organic nutrient gradient and microbial competition inside the BAC-filter. Increase in the ozone dose could further reduce the OPs at the bottom of the BAC-filter. Spearman correlation analysis demonstrated that some significant correlations existed between target microorganisms, suggesting potential microbial ecological relationships. Overall, our results demonstrated that the BAC-filter may act as a "battlefield"suppressing the OPs through microbial competition. O_3-BAC-Cl_2 could be an effective multibarrier process to suppress the proliferation of OPs in the bulk water of DWDS. However, OPs protected by the biofilms of DWDS should receive further attention because OPs may be detached and released from the biofilms.展开更多
文摘采用Illumina MiSeq高通量测序技术,对臭氧-生物活性炭水处理工艺过程各单元出水中细菌多样性及丰度进行研究,测序获得196 809条16S r DNA基因序列,归类为38个门,522个属。各样品中细菌多样性分析结果表明:在各处理单元出水中细菌群落具有高度多样性,预臭氧和臭氧氧化处理对水体中细菌多样性及丰度的影响最大,可杀灭一部分属的细菌,可合理控制臭氧浓度杀灭部分耐氯菌;絮凝沉淀和沙滤单元处理对水体中细菌多样性具有恢复效果,使水体中细菌种属进一步增多;在生物活性炭滤池处理后,细菌多样性增加,丰度分布更为均匀,对后期消毒工艺提出了更高的要求。
基金supported by the National Key Research and Development Plan (No.2016YFA0203200)the National Natural Science Foundation of China (No.51538013)the project of Chinese Academy of Sciences (No.QYZDY-SSW-DQC004)
文摘Simulated drinking water distribution system(DWDS) treated with O_3-BAC-Cl_2(ozonebiological activated carbon-chlorine) was constructed to study its effects on the regrowth of five typical opportunistic pathogens(OPs). It was found that O_3-BAC-Cl_2 could significantly reduce the regrowth of target OPs in the effluents of DWDS compared with Cl_2 and O_3-Cl_2 with the same residual chlorine levels. However, the effect of O_3-BAC-Cl_2 on the average numbers of target OPs gene markers in the biofilms of DWDS was not apparent, suggesting that OPs in the biofilms of DWDS were tolerant to the upstream disinfection process. The quantification of target OPs in the BAC-filter column demonstrated that OPs decreased with the increase of depth, which was likely due to the organic nutrient gradient and microbial competition inside the BAC-filter. Increase in the ozone dose could further reduce the OPs at the bottom of the BAC-filter. Spearman correlation analysis demonstrated that some significant correlations existed between target microorganisms, suggesting potential microbial ecological relationships. Overall, our results demonstrated that the BAC-filter may act as a "battlefield"suppressing the OPs through microbial competition. O_3-BAC-Cl_2 could be an effective multibarrier process to suppress the proliferation of OPs in the bulk water of DWDS. However, OPs protected by the biofilms of DWDS should receive further attention because OPs may be detached and released from the biofilms.
