为了了解屠宰场大肠杆菌O26污染情况,参考美国农业部(united states department of agriculture,USDA)的检测方法,对样品进行选择性增菌,经免疫磁珠富集、选择性显色培养基mRainbow Agar分离纯化后,挑选可疑菌株采用PCR方法鉴定O抗原,...为了了解屠宰场大肠杆菌O26污染情况,参考美国农业部(united states department of agriculture,USDA)的检测方法,对样品进行选择性增菌,经免疫磁珠富集、选择性显色培养基mRainbow Agar分离纯化后,挑选可疑菌株采用PCR方法鉴定O抗原,进一步采用血清凝集试验进行验证,并对确认的阳性菌株采用多重PCR方法进行毒力基因(stx1、stx2、eae、hly)检测。结果表明,采集的120份样品中共分离到1株大肠杆菌O26,但上述4种毒力基因均为阴性。该屠宰场存在大肠杆菌O26的污染,但分离出的大肠杆菌O26没有携带毒力基因。展开更多
A new isopolyoxovanadate [Ni(1,10′-phen) 3] 2 [V Ⅳ 2V Ⅴ 8O 26] was hydrothermally synthesized and structurally characterized by the single crystal X-ray diffraction. The compound crystallizes in orthorhombic system...A new isopolyoxovanadate [Ni(1,10′-phen) 3] 2 [V Ⅳ 2V Ⅴ 8O 26] was hydrothermally synthesized and structurally characterized by the single crystal X-ray diffraction. The compound crystallizes in orthorhombic system, space group Pbcn with a=1.362 9(3) nm, b=2.215 6(4) nm, c= 2.571 2(4) nm, V=7.764(2) nm 3, Z=4, R=0.040 3, wR 2=0.044 6. The compound exhibits an unusual empty ellipsoidal cage, which is constructed by eight {V ⅤO 4} tetrahedra and two {V ⅣO 5} square pyramids via corner-sharing modes. The chiral [Ni(1,10′-phen) 3] 2+ complexes act as the counter cations.展开更多
文摘为了了解屠宰场大肠杆菌O26污染情况,参考美国农业部(united states department of agriculture,USDA)的检测方法,对样品进行选择性增菌,经免疫磁珠富集、选择性显色培养基mRainbow Agar分离纯化后,挑选可疑菌株采用PCR方法鉴定O抗原,进一步采用血清凝集试验进行验证,并对确认的阳性菌株采用多重PCR方法进行毒力基因(stx1、stx2、eae、hly)检测。结果表明,采集的120份样品中共分离到1株大肠杆菌O26,但上述4种毒力基因均为阴性。该屠宰场存在大肠杆菌O26的污染,但分离出的大肠杆菌O26没有携带毒力基因。
文摘A new isopolyoxovanadate [Ni(1,10′-phen) 3] 2 [V Ⅳ 2V Ⅴ 8O 26] was hydrothermally synthesized and structurally characterized by the single crystal X-ray diffraction. The compound crystallizes in orthorhombic system, space group Pbcn with a=1.362 9(3) nm, b=2.215 6(4) nm, c= 2.571 2(4) nm, V=7.764(2) nm 3, Z=4, R=0.040 3, wR 2=0.044 6. The compound exhibits an unusual empty ellipsoidal cage, which is constructed by eight {V ⅤO 4} tetrahedra and two {V ⅣO 5} square pyramids via corner-sharing modes. The chiral [Ni(1,10′-phen) 3] 2+ complexes act as the counter cations.
