Background The deposition of intramuscular fat(IMF)in livestock can enhance the flavor and tenderness of meat products,significantly increasing consumer satisfaction.To achieve this industrial trait,this study investi...Background The deposition of intramuscular fat(IMF)in livestock can enhance the flavor and tenderness of meat products,significantly increasing consumer satisfaction.To achieve this industrial trait,this study investigated the regulatory effects of 20 dietary nutrients on sheep IMF deposition using a 3D organoid culture model.Results Key nutrients enhancing angiogenesis,adipocyte differentiation,and lipid accumulation were identified through assessments of capillary sprouts development,mRNA expression,and Oil Red O staining.Vitamins C(VC),E(VE),and K1(VK1),guanidinoacetic acid(GAA),leucine(Leu),lysine(Lys),methionine(Met),N-carbamylglutamate(NCG),tryptophan(Trp),α-linolenic acid(ALA),linoleic acid(LA),cis-9,trans-11 conjugated linoleic acid(c9,t11-CLA),acetic acid(HAc),and sodium acetate(NaAc)stimulated while vitamins B9(VB9),D(VD),K2(VK2),taurine(Tau),and sodium butyrate(NaBu)inhibited angiogenesis(P<0.05).Furthermore,VC,VE,VK1,VK2,GAA,Leu,NCG,Trp,ALA,LA,and HAc enhanced adipocyte differentiation,with VE,VK1,GAA,Leu,LA,and HAc additionally elevating lipid accumulation(P<0.05).Conclusions Various nutrients play distinct regulatory roles in angiogenesis,adipocyte differentiation,and lipid accumulation.These findings provide a roadmap for further optimizing the production of marbled meat through nutritional intervention in actual livestock breeding production.展开更多
Understanding the spatial distributions and corresponding variation mechanisms of key soil nutrients in fragile karst ecosystems can assist in promoting sustainable development.However,due to the implementation of eco...Understanding the spatial distributions and corresponding variation mechanisms of key soil nutrients in fragile karst ecosystems can assist in promoting sustainable development.However,due to the implementation of ecological restoration initiatives such as land-use conversions,novel changes in the spatial characteristics of soil nutrients remain unknown.To address this gap,we explored nutrient variations and the drivers of the variation in the 0–15 cm topsoil layer using a regional-scale sampling method in a typical karst area in northwest Guangxi Zhuang Autonomous Region,Southwest China.Descriptive statistics,geostatistics,and spatial analysis were used to assess the soil nutrient variability.The results indicated that soil organic carbon(SOC),total nitrogen(TN),total phosphorus(TP),and total potassium(TK)concentrations showed moderate variations,with coefficients of variance being 0.60,0.60,0.71,and 0.72,respectively.Moreover,they demonstrated positive spatial autocorrelations,with global Moran's indices being 0.68,0.77,0.64,and 0.68,respectively.However,local Moran's index values were low,indicating large spatial variations in soil nutrients.The best-fitting semi-variogram models for SOC,TN,TP,and TK concentrations were spherical,Gaussian,exponential,and exponential,respectively.According to the classification criteria of the Second National Soil Census in China,SOC and TN concentrations were relatively sufficient,with the proportions of rich and very rich levels being up to 90.9 and 96.0%,respectively.TP concentration was in the mediumdeficient level,with the areas of medium and deficient levels accounting for 33.7 and 30.1%of the total,respectively.TK concentration was deficient,with the cumulative area of extremely deficient,very deficient,and deficient levels accounting for 87.6%of the total area.Consequently,the terrestrial ecosystems in the study area were more vulnerable to soil P and K than soil N deficiencies.Furthermore,variance partitioning analysis of the influencing factors showed that,except for the interactions,the single effect of other soil properties accounted more for soil nutrient variations than spatial and environmental variables.These results will aid in the future management of terrestrial ecosystems.展开更多
Nitrogen(N)and phosphorus(P)are essential nutrients and can significantly impact primary productivity of the ecosystem causing water environmental problems.However,their cycling mechanisms are not well understood in a...Nitrogen(N)and phosphorus(P)are essential nutrients and can significantly impact primary productivity of the ecosystem causing water environmental problems.However,their cycling mechanisms are not well understood in alpine mountains with climate change.Hence,94 samples of river water were collected from 2018 to 2020 in the headwaters of the Shule River Basin to assess the nutrients spatiotemporal distribution and combined ap-proach of water quality index to assess water quality and potential sources.The findings depict that high nutrient concentrations were found to coincide with snowmelt and glacial meltwater and rainfall recharge periods,while total flux peaked from June to September due to increased runoff.Notably,total nitrogen(TN)concentrations were significantly higher near the town,primarily attributed to the replenishment of nitrate(NO_(3)^(‒)-N)from live-stock manure.The high total P(TP)was near the glacier,which was attributed to the transportation of glacial sediments into the river,and pH was another critical factor.N was the primary nutrient limiting factor for the growth of phytoplankton in river water.Although the migration and transport of nutrients have altered with climate change,river water quality is good in alpine mountains based on an overall evaluation.These findings contribute to enriching nutrient datasets and highlight the importance of water resource management and water quality assessment in sensitive and fragile alpine mountains.展开更多
Nutrients play a crucial role in sustaining marine ecosystems and supporting mariculture,especially in seaweed aquaculture.Currently,seaweed farming,such as kelp cultivation,is entirely dependent on the natural supply...Nutrients play a crucial role in sustaining marine ecosystems and supporting mariculture,especially in seaweed aquaculture.Currently,seaweed farming,such as kelp cultivation,is entirely dependent on the natural supply of nutrients.Sanggou Bay in Shandong Peninsula,Yellow Sea,is renowned for its 60-year history of kelp cultivation;however,it is recently facing an increasing demand for nitrogen and phosphorus due to the expansion in aquaculture scale and production.There is no doubt that nutrient addition can enhance and sustain the production,but it is crucial to understand its effect on kelp growth under current nutrients condition and the potential ecological risks.Our in-field nutrient enrichment experiments show that nitrogen and phosphorus additions promoted the kelp biomass during the early growth stages,and have no adverse effects on phytoplankton or seawater nutrient levels throughout the experiment.From a long-term perspective,increasing nutrient supply appears to be an essential strategy for sustaining the aquaculture of kelp.展开更多
Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development...Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development of mechanization,wide-row spacing substrate cultivation became an optimize mode of the greenhouse cucumber cultivation,aligning with the trend of intelligent agriculture.To determine the optimal nutrient solution supply amount(NS)and supply frequency(SF)for promoting the integrated growth of cucumber under wide-row spacing substrate cultivation,we explored the effects of substrate supply amount(SS),NS,and SF on cucumber yield,quality,and element utilization efficiency.A five-level quadratic orthogonal rotation combination design with three experimental factors(NS,SF,and SS)was implemented for 23 coupling treatments over three growing seasons,including spring(2022S and 2023S)and autumn(2022A).The technique for order preference by similarity to ideal solution(TOPSIS)combining weights based on game theory was applied to construct cucumber comprehensive growth evaluation model.Single and two experimental factors analyses revealed significant effects of single factors and the coupling of NS-SS,NS-SF and SS-SF on the integrated growth of cucumber for all three growing seasons.For the NS-SF-SS combination,the optimal parameters for comprehensive cucumber growth were determined as follows:levels of^(-1).68 for NS,-0.7 for SF,and^(-1).682 for SS in 2022A;-0.43 for NS,-0.06 for SF,and 0.34 for SS in 2022S;0.3 for NS,-0.02 for SF,and 0.04 for SS in 2023S.Furthermore,for SS ranges of 2.00-3.01,3.01-4.50,4.50-5.99,5.99-7.00(L·plant^(-1)),the corresponding NS and SF intervals maximizing cucumber integrated growth in spring were:0.28-0.30(L·plant^(-1))and 6(times·d^(-1)),0.26-0.30(L·plant^(-1))and 6(times·d^(-1)),0.25-0.30(L·plant^(-1))and 6(times·d^(-1)),0.23-0.30(L·plant^(-1))and 6(times·d^(-1)),respectively.With the same SS,the corresponding NS and SF intervals that maximized cucumber integrated growth in autumn were:0.10(L·plant^(-1))and 8(times·d^(-1)),0.18(L·plant^(-1))and 7(times·d^(-1)),0.30(L·plant^(-1))and 6(times·d^(-1)),0.49(L·plant^(-1))and 5(times·d^(-1)),respectively.The results provide a theoretical basis for solution management,and further in-depth research on cucumber cultivation.展开更多
Improving crop productivity and soil fertility through the balanced application of inorganic and organic nutrient sources is a sustainable approach in modern agriculture.Char land soils,widely distributed in riverine ...Improving crop productivity and soil fertility through the balanced application of inorganic and organic nutrient sources is a sustainable approach in modern agriculture.Char land soils,widely distributed in riverine Bangladesh,are generally low in organic matter status and deficient in necessary nutrient elements for crop production.Addressing this challenge,the present study was conducted to investigate the effects of various organic nutrient sources with inorganic fertilizers on crop yields,nutrient uptake,and soil fertility in farm(L1)and char land(L2)of Brahmaputra River in Mymensingh,Bangladesh from 2022(Y1)to 2023(Y2).For each location,eight treatments viz.T1(Control),T2[100%recommended fertilizer dose(RFD)],T3(75%RFD),T4(75%N from RFD 25%N from cow dung),T5(75%+N from RFD 25%N from poultry manure),T6(75%N from RFD 25%N from vermicompost),T7(75%N from++RFD 25%N from household compost)and T8(75%N from RFD 25%N from rice straw compost)were arranged in++a randomized complete block design with three replications using Wheat–Mungbean–T.Aman rice cropping pattern where three way interaction was considered for results.Treatment T5 performed the best in both years in both locations as it enhanced the yield components(p 0.05)and caused yield increment over control.The yield improvement in<Char land soils was higher than that in farm soils.For all three crops,treatment T5 consistently augmented the uptake of nitrogen,phosphorus,potassium,and sulphur by different parts of the crops and improved soil fertility properties such as organic matter status,cation exchange capacity,total nitrogen,available phosphorus,and sulphur as well as exchangeable potassium in both locations in both years.Cost and return analysis of different treatments for the whole cropping system showed that the highest marginal benefit-cost ratio(16.35 and 15.07)and gross return(about Tk 768,595/ha and 728,341/ha)were obtained from the T5 treatment in farm soils and Char land soils,respectively.Followed by poultry manure,vermicompost performed well in addition to mineral fertilizers for improving crop yield and soil fertility but its economic efficiency was less due to high input cost.These findings may be useful to the smallholder farmers in char areas,who could benefit from increased productivity,reduced reliance on chemical fertilizers,and improved soil health,contributing to the long-term sustainability of char land agriculture.展开更多
To better understand the effects of ground-level ozone(O_(3))on nutrients and stoichiometry in different plant organs,urban tree species Celtis sinensis,Cyclocarya paliu-rus,Quercus acutissima,and Quercus nuttallii we...To better understand the effects of ground-level ozone(O_(3))on nutrients and stoichiometry in different plant organs,urban tree species Celtis sinensis,Cyclocarya paliu-rus,Quercus acutissima,and Quercus nuttallii were sub-jected to a constant exposure to charcoal-filtered air(CF),nonfiltered air(NF),or NF+40,60,or 80 nmol O_(3)mol^(-1)(NF40,NF60,and NF80)starting early in the summer of the growing season.At the end of summer,net CO_(2)assimila-tion rate(A),stomatal conductance(gs),leaf mass per area(LMA),and/or leaf greenness(SPAD)either were not sig-nificantly affected by elevated O_(3)or were even higher in some cases during the summer compared with the CF or NF controls.LMA was significantly lower in autumn only after the highest O_(3)exposures.Compared to NF,NF40 caused a large increase in gs across species in late summer and more K and Mn in stems.At the end of the growing season,nutri-ent status and stoichiometric ratios in different organs were variously altered under O_(3)stress;many changes were large and often species-specific.Across O_(3)treatments,LMA was primarily associated with C and Mg levels in leaves and Ca levels in leaves and stems.NF40 enriched K,P,Fe,and Mn in stems,relative to NF,and NF60 enhanced Ca in leaves relative to CF and NF40.Moreover,NF resulted in a higher Ca/Mg ratio in leaves of Q.acutissima only,relative to the other O_(3)regimes.Interestingly,across species,O_(3)stress led to different nutrient modifications in different organs(stems+branches vs leaves).Thus,ambient and/or elevated O_(3)exposures can alter the dynamics and distribution of nutrients and disrupt stoichiometry in different organs in a species-specific manner.Changes in stoichiometry reflect an important defense mechanism in plants under O_(3),and O_(3)pollution adds more risk to ecological stoichiometries in urban areas.展开更多
While many harmful algal blooms have been associated with increasing eutrophication, not all species respond similarly and the increasing challenge, especially for resource managers, is to determine which blooms are r...While many harmful algal blooms have been associated with increasing eutrophication, not all species respond similarly and the increasing challenge, especially for resource managers, is to determine which blooms are related to eutrophication and to understand why particular species proliferate under specific nutrient conditions. The overall goal of this brief review is to describe why nutrient loads are not changing in stoichiometric proportion to the "Redfield ratio", and why this has important consequences for algal growth. Many types of harmful algae appear to be able to thrive, and/or increase their production of toxins, when nutrient loads are not in proportion classically identified as Redfield ratios. Here we also describe some of the physiological mechanisms of different species to take up nutrients and to thrive under conditions of nutrient imbalance.展开更多
Background There is a growing focus on using various plant-derived agricultural by-products to increase the benefits of pig farming,but these feedstuffs are fibrous in nature.This study investigated the relationship b...Background There is a growing focus on using various plant-derived agricultural by-products to increase the benefits of pig farming,but these feedstuffs are fibrous in nature.This study investigated the relationship between dietary fiber physicochemical properties and feedstuff fermentation characteristics and their effects on nutrient utilization,energy metabolism,and gut microbiota in growing pigs.Methods Thirty-six growing barrows(47.2±1.5 kg)were randomly allotted to 6 dietary treatments with 2 apparent viscosity levels and 3β-glucan-to-arabinoxylan ratios.In the experiment,nutrient utilization,energy metabolism,fecal microbial community,and production and absorption of short-chain fatty acid(SCFA)of pigs were investigated.In vitro digestion and fermentation models were used to compare the fermentation characteristics of feedstuffs and ileal digesta in the pig’s hindgut.Results The production dynamics of SCFA and dry matter corrected gas production of different feedstuffs during in vitro fermentation were different and closely related to the physical properties and chemical structure of the fiber.In animal experiments,increasing the dietary apparent viscosity and theβ-glucan-to-arabinoxylan ratios both increased the apparent ileal digestibility(AID),apparent total tract digestibility(ATTD),and hindgut digestibility of fiber components while decreasing the AID and ATTD of dry matter and organic matter(P<0.05).In addition,increasing dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios both increased gas exchange,heat production,and protein oxidation,and decreased energy deposition(P<0.05).The dietary apparent viscosity andβ-glucanto-arabinoxylan ratios had linear interaction effects on the digestible energy,metabolizable energy,retained energy(RE),and net energy(NE)of the diets(P<0.05).At the same time,the increase of dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios both increased SCFA production and absorption(P<0.05).Increasing the dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios increased the diversity and abundance of bacteria(P<0.05)and the relative abundance of beneficial bacteria.Furthermore,increasing the dietaryβ-glucan-to-arabinoxylan ratios led to a linear increase in SCFA production during the in vitro fermentation of ileal digesta(P<0.001).Finally,the prediction equations for RE and NE were established.Conclusion Dietary fiber physicochemical properties alter dietary fermentation patterns and regulate nutrient utilization,energy metabolism,and pig gut microbiota composition and metabolites.展开更多
Diel investigations of water environments are one means to holistically understand the dynamics and functional roles of phytoplankton,bacteria and viruses in these ecosystems.They have the potential to substantially i...Diel investigations of water environments are one means to holistically understand the dynamics and functional roles of phytoplankton,bacteria and viruses in these ecosystems.They have the potential to substantially impact carbon(C),nitrogen(N)and phosphorus(P)biogeochemistry through their respective roles.This study characterizes the phytoplankton,bacteria and virus communities and the elemental composition of various C,N and P nutrients flow over three diel cycles in tropical urban lake.Our results show that ratios of C:N:P fluctuated strongly from the lack of dissolved organic phosphorus(DOP)and PO_(4).Specifically,green algae peaked during day time and exudate dissolved organic matter(DOM)that strongly modulate dissolved organic carbon(DOC):DOP ratio to diel DOP limitation.Multiple linear regression and Stella modelling emphasize the roles of viruses together with Synechococcus as important nutrient recyclers of NH_(4)and PO_(4)in nutrients-limited waters.Respective normalised surface PO_(4)and combined surface and bottom NH_(4)concentration selected both viruses and Synechococcus as important drivers.Process model of N and P biogeochemical cycles can achieve 69%and 57%similar to observed concentration of NH_(4)and PO_(4),respectively.A short latent period of 9 hr was calculated,in addition to the calibrated high infectivity of viruses to Synechococcus.Taken together,the rapid turn-over between Synechococcus and viruses has biogeochemical significance,where the rapid recycling of essential nutrients allows for shortcuts in the N and P cycle,supporting a wide range of microbes.展开更多
With the rapid growth of the global population and the increasing demand for healthier diets,improving the nutrient utilization efficiency of staple food crops has become a critical scientific and industrial chal-leng...With the rapid growth of the global population and the increasing demand for healthier diets,improving the nutrient utilization efficiency of staple food crops has become a critical scientific and industrial chal-lenge,prompting innovation in food processing technologies.This review introduces first the common nutritional challenges in the processing of staple food crops,followed by the comprehensive examination of research aiming to enhance the nutritional quality of staple food crop-based foods through innovative processing technologies,including microwave(MW),pulsed electric field(PEF),ultrasound,modern fer-mentation technology,and enzyme technology.Additionally,soybean processing is used as an example to underscore the importance of integrating innovative processing technologies for optimizing nutrient utilization in staple food crops.Although these innovative processing technologies have demonstrated a significant potential to improve nutrient utilization efficiency and enhance the overall nutritional pro-file of staple food crop-based food products,their current limitations must be acknowledged and addressed in future research.Fortunately,advancements in science and technology will facilitate pro-gress in food processing,enabling both the improvement of existing techniques as well as the develop-ment of entirely novel methodologies.This work aims to enhance the understanding of food practitioners on the way processing technologies may optimize nutrient utilization,thereby fostering innovation in food processing research and synergistic multi-technological strategies,ultimately providing valuable references to address global food security challenges.展开更多
To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conduct...To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conducted at 0,1,3,and 5 days before harvest.The appearance,respiration rate,mass-loss rate,electrolyte leakage,glucosinolate,ascorbic acid,total phenol,total flavonoid,total sugar and sucrose contents,and sucrose phosphate synthase(SPS),invertase(INV),sucrose synthase synthesis(SSS)and cleavage(SSC)activities of broccoli samples were observed after 0,2,4,6,8,and 10 days of storage.The results showed that spraying arginine at 5 days preharvest(5-ARG)helped to inhibit broccoli respiration during storage,delay electrolyte leakage,and maintain broccoli color.Furthermore,during the growth stage,total sugar accumulation was higher in the 5-ARG group.In addition,during the storage period,sucrose synthesis was accelerated,while sucrose cleavage was inhibited,resulting in more sucrose retention in postharvest broccoli.In conclusion,5-ARG resulted in the accumulation of more nutrients during the growth process and effectively delayed the quality decline during storage,thereby prolonging the shelf life of broccoli.Therefore,this study provides a theoretical basis for improving postharvest storage characteristics of broccoli through preharvest treatments.展开更多
The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, ...The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, total P and C: N ratio of the soil inP. sylvestris var.mongolica stands were significantly higher in comparison with those inP. tabulaeformis andP. densiflora stands. ForP. sylvestris var.mongolica, the foliar P concentration appeared to decrease with age, and the foliar N and K concentrations did not show a consistent change with age. As for the different tree species of the similar age, the foliar N and P concentrations were significantly different (p<0.05), being withP. sylvestris var.mongolica>P. densiflora>P. tabulaeformis. The foliar N: P ratio ofP. densiflora significantly was higher thanP. sylvestris var.mongolica andP. tabulaeformis, while the foliar K was no obvious difference between the three tree species. There were significant correlation (p<0.05) between soil total N and P, soil organic matter and total P, foliar N and P, but it did not show significant correlations between soil and foliar nutrient concentrations, which might attribute to the excessive litter raking, overgrazing and low soil moisture in this area. Based on the foliar N: P ratio, we introduced a combination threshold index of N: P ratio with their absolute foliar nutrient concentrations to determine the possible limiting nutrient. According to the critical N: P ratio and their absolute foliar N, P concentrations, theP. sylvestris var.mongolica stands showed a decreased N limitation degree with age, theP. densiflora stands showed unlimited by N and P in the whole, and theP. tabulaeformis stands showed co-limited by N and P. No significant difference in soil nutrient concentrations of the surface soils was found between 45, 29, 20-yr-oldPinus sylvestris var.mongolica plantation stands. Keywords coniferous trees - foliar nutrient concentration - limiting nutrients - N - P ratio - Zhanggutai sandy land CLC number S718.55 Document code A Article ID 1007-662X(2004)01-0011-08 Foundation item: This research was supported by Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.Biography: CHEN Guang-sheng (1978-), male, master candidate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Song Funan展开更多
A study was conducted to evaluate the soil nutrient status of poplar plantation by Soil Nutrient Systematic Approach (SNSA) in Jianghan Plain, Hubei Province, China. Soil physiochemical properties were analyzed in lab...A study was conducted to evaluate the soil nutrient status of poplar plantation by Soil Nutrient Systematic Approach (SNSA) in Jianghan Plain, Hubei Province, China. Soil physiochemical properties were analyzed in laboratory through collection soil samples of study site. Ten treatments of application different fertilizers were designed such as CK, optimum treatment (N, P, K, Zn), N(P, K, Zn), P(N, K, Zn), K(N, P, Zn), +Mg(N, P, K, Zn, Mg), Zn (N,P,K), +2P(N, 2P, K, Zn), +2K(N, P, 2K, Zn), and 2N+2P+2K(2N, 2P, 2K, Zn) for field experiment to test the effect on tree height, diameter (DBH) growth, and dry weight of poplar. The results showed that there was no significant difference in tree heights between treatments with different fertilizers, diameter growth of poplar trees in treatments of lack of N and Zn was significantly slower than that of trees in optimum treatment, and dry weight of poplar dropped significantly for treatment of CK as well as treatments without application N and Zn. It is concluded that N and Zn were main limiting factor for poplar growth. Results from laboratory analysis and field experiment were uniform per-fectly, which proved that SNSA was reliable in evaluating soil nutrient status of poplar plantation.展开更多
A field trial to evaluate the growth and nutrient uptake of cocoa intercropped with coconut was conducted in Cocoa Research Institute of Nigeria(CRIN)Headquarters,Idi-Ayunre,Ibadan,Oyo State between 2019 and 2022.Ther...A field trial to evaluate the growth and nutrient uptake of cocoa intercropped with coconut was conducted in Cocoa Research Institute of Nigeria(CRIN)Headquarters,Idi-Ayunre,Ibadan,Oyo State between 2019 and 2022.There were four treatments comprising of cocoa sole,cocoa/plantain,cocoa/coconut and cocoa/coconut/plantain intercrop.The experimental design was Randomized Complete Block(RCB)replicated three times.Result obtained showed that cocoa/plantain was significantly higher(p<0.05)than all other treatments in all parameters considered and closely followed by cocoa/coconut,then cocoa sole with cocoa/plantain/coconut giving the least performance.The same trend was observed in the nutrient uptake.It was also observed in the trial that the two crops:cocoa and coconut did not show any deleterious effect on their growth when intercropped together.This showed that the two crops are compatible when grown on the same piece of land.Therefore,cocoa/coconut intercrop could be recommended to cocoa farmers in south west Nigeria to increase their revenue generation when coconut starts bearing fruit instead of sole planting of cocoa.展开更多
Selenium(Se)is a nutrient that is considered beneficial for plants,because its improvement in growth,yield and quality helps plants to mitigate stress.The objective of this research was to evaluate the application of ...Selenium(Se)is a nutrient that is considered beneficial for plants,because its improvement in growth,yield and quality helps plants to mitigate stress.The objective of this research was to evaluate the application of sodium selenite(Na2SeO3),nanoparticles(SeNPs)and microparticles(SeMPs)of Se in cucumber seedlings,via two experiments:one with seed priming and the other with foliar application of Sematerials.The doses used were:0,0.1,0.5,1.0,1.5 and 3.0 mg⋅L^(−1),for each form of Se and for each form of application.Treatment 0 consisted of the application of distilled water,which was used as a control.The results indicated that the SeMPs treatment at 3.0 mg⋅L^(−1)for seed priming had the greatest effect on stem diameter and leaf area.Foliar application of SeMPs at 1.5 mg⋅L^(−1)was the most effective at increasing the leaf area.In terms of fresh and dry biomass(aerial,root and total)for seed priming,all the treatments were superior to the control,and SeMPs at 1.5 and 3.0 mg⋅L^(−1)caused the greatest effects.With foliar application,fresh root biomass improved to a greater extent with the SeMPs treatment at 3.0 mg⋅L^(−1),and dry biomass(aerial,root and total)increased with the SeMPs at 1.0 and 3.0 mg⋅L^(−1).With respect to the photosynthetic pigments,proteins,phenols and minerals,the Se treatments,both for seed priming and foliar application,caused increases and decreases;however,reduced glutathione(GSH)increased with treatments in both forms of application.The Se concentration in the seedlings increased as the dose of Se material increased,and greater accumulation was achieved with foliar application of SeNPs and SeMPs.The results indicate that the use of Se materials is recommended,mainly the use of SeMPs,which improved the variables studied.This opens new opportunities for further studies with SeMPs,as little information is available on their application in agricultural crops.展开更多
Afforestation on formerly cultivated or aban-doned agricultural land is a common strategy to increase forest areas and enhance carbon sequestration.Deep soil ploughing before afforestation improves soil conditions,fac...Afforestation on formerly cultivated or aban-doned agricultural land is a common strategy to increase forest areas and enhance carbon sequestration.Deep soil ploughing before afforestation improves soil conditions,facilitating tree growth and carbon storage.This study assessed the growth and biomass parameters of Pinus sylves-tris in 10-and 20 years old plantations established on deeply ploughed and non-ploughed soils in Lithuania.Biomass allocation and carbon and nutrient concentrations including N,P,K,Ca and Mg were analysed in aboveground biomass components.Deep ploughing in the 10 years old stands negatively impacted vertical growth and stem development but did not significantly affect overall biomass accumula-tion.In contrast,in the 20 years old stands,deep plough-ing resulted in taller trees with larger diameters and higher biomass accumulation compared to non-ploughed sites.Biomass distribution within tree canopies varied between ploughed and non-ploughed sites,indicating diverse effects of deep ploughing.Carbon and nutrient concentrations in biomass components showed site-specific variations,with deep ploughing influencing carbon concentrations in needles and stem bark.Overall,deep ploughing showed potential for enhancing tree growth and biomass accumulation,with implications for carbon sequestration in forest ecosystems.These findings help us understand the impact of an alternative soil management practice,deep ploughing,on forest development and carbon dynamics.展开更多
Background Progressive oxidative stress easily occurs as a result of a gradual increase in the intensity of maternal metabolism due to rapid foetal development and increased intensity of lactation.However,studies on t...Background Progressive oxidative stress easily occurs as a result of a gradual increase in the intensity of maternal metabolism due to rapid foetal development and increased intensity of lactation.However,studies on the effects of processive oxidative stress on nutrient transport in the placenta have received little attention.The present study was conducted on sows at 85 days of gestation to study the effects of pterostilbene(PTE)on maternal oxidative stress status and placental nutrient transport.Results PTE increased the antioxidant capacity and immunoglobulin content in mothers’blood and milk,reduced the level of inflammatory factors,and improved the nutrient content of milk.PTE also reduced sow backfat loss and the number of weak sons,and increased piglet weaning weight and total weaning litter weight.We subsequently found that PTE enhanced placental glucose and fatty acid transport and further affected glycolipid metabolism by increasing the expression of LAL,PYGM,and Gbe-1,which activated the PI3K phosphorylation pathway.Moreover,PTE addition altered the relative abundance of the Firmicutes,Proteobacteria,Parabacillus,and Bacteroidetes-like RF16 groups in sow faeces.PTE increased the levels of acetate,propionate,butyrate and isovalerate in the faeces.Conclusions These findings reveal that the addition of PTE during pregnancy and lactation mitigates the effects of processive oxidative stress on offspring development by altering maternal microbial and placental nutrient trans-port capacity.展开更多
The degradation of animal carcasses can lead to rapid waste release(e.g.,pathogenic bacteria,viruses,prions,or parasites)and also result in nutrient accumulation in the surrounding environment.However,how viral profil...The degradation of animal carcasses can lead to rapid waste release(e.g.,pathogenic bacteria,viruses,prions,or parasites)and also result in nutrient accumulation in the surrounding environment.However,how viral profile responds and influences nutrient pool(carbon(C),nitrogen(N),phosphorus(P)and sulfur(S))in polluted water caused by animal carcass decomposition had not been explored.Here,we combined metagenomic analysis,16S rRNA gene sequencing and water physicochemical assessment to explore the response of viral communities under different temperatures(23℃,26℃,29℃,32℃,and 35℃)in water polluted by cadaver,as well as compare the contribution of viral/bacterial communities on water nutrient pool.We found that a total of 15,240 viral species were classified and mainly consisted of Siphoviridae.Both temperature and carrion reduced the viral diversity and abundance.Only a small portion of the viruses(∼8.8%)had significant negative correlations with temperature,while most were not sensitive.Our results revealed that the viruses had lager contribution on nutrient pool than bacteria.Besides,viral-related functional genes involved in C,N,P and S cycling.These functional genes declined during carcass decomposition and covered part of the central nutrient cycle metabolism(including carbon sugar transformation,denitrification,P mineralization and extracelluar sulfate transfer,etc.).Our result implies that human regulation of virus communities may be more important than bacterial communities in regulating and managing polluted water quality and nutrition.展开更多
Tropical coastal ecosystems,vital habitats for economically significant marine species,face increasing threats from eutrophication driven by human-induced nutrient pollution.This study investigated the metabolomic pro...Tropical coastal ecosystems,vital habitats for economically significant marine species,face increasing threats from eutrophication driven by human-induced nutrient pollution.This study investigated the metabolomic profile of Anadara granosa,a marine bivalve,to identify metabolites indicative of eutrophic conditions.Conducted in Cirebon Bay,Indonesia,the research spanned five locations reflecting varying eutrophication levels.Bottom-layer water samples were analyzed for pH,dissolved oxygen,salinity,water transparency,and nutrient concentrations(phosphate,nitrate,ammonia).Proton-Nuclear Magnetic Resonance(Proton-NMR)metabolomics of A.granosa revealed metabolite variations linked to water quality.Eutrophic conditions,prevalent in the bay’s northern side,were characterized by inorganic nitrogen levels above 0.2 mg L^(-1) and phosphate exceeding 0.024 mg L^(-1).These were linked to urban,aquaculture,and agricultural runoff,driving increased phytoplankton biomass and reduced salinity variability.Metabolomic analysis identified inosine and dimethylglycine as potential biomarkers.Elevated inosine levels in eutrophic environments suggest increased cellular activity due to greater food availability,while higher dimethylglycine concentrations detected in low-nutrient as its role in osmotic regulation.Both metabolites correlated with ammonia concentrations(P<0.05,R^(2)=0.631),a key contributor to eutrophication.These findings highlight inosine and dimethylglycine as effective indicators of nutrient enrichment,providing insights into eutrophication’s impact on coastal ecosystems and A.granosa’s physiological responses.展开更多
基金supported by the Key Research and Development Program-Key Projects(2021YFD1200900,2023YFD1301302)the grants from the National Natural Science Foundation of China(32272892,32472886)+2 种基金the Bayannaoer Research Institute Young Scientist Project(2024BYNECAU003)the Chinese Universities Scientific Fund(2025TC101)the 2115 Talent Development Program of China Agricultural University。
文摘Background The deposition of intramuscular fat(IMF)in livestock can enhance the flavor and tenderness of meat products,significantly increasing consumer satisfaction.To achieve this industrial trait,this study investigated the regulatory effects of 20 dietary nutrients on sheep IMF deposition using a 3D organoid culture model.Results Key nutrients enhancing angiogenesis,adipocyte differentiation,and lipid accumulation were identified through assessments of capillary sprouts development,mRNA expression,and Oil Red O staining.Vitamins C(VC),E(VE),and K1(VK1),guanidinoacetic acid(GAA),leucine(Leu),lysine(Lys),methionine(Met),N-carbamylglutamate(NCG),tryptophan(Trp),α-linolenic acid(ALA),linoleic acid(LA),cis-9,trans-11 conjugated linoleic acid(c9,t11-CLA),acetic acid(HAc),and sodium acetate(NaAc)stimulated while vitamins B9(VB9),D(VD),K2(VK2),taurine(Tau),and sodium butyrate(NaBu)inhibited angiogenesis(P<0.05).Furthermore,VC,VE,VK1,VK2,GAA,Leu,NCG,Trp,ALA,LA,and HAc enhanced adipocyte differentiation,with VE,VK1,GAA,Leu,LA,and HAc additionally elevating lipid accumulation(P<0.05).Conclusions Various nutrients play distinct regulatory roles in angiogenesis,adipocyte differentiation,and lipid accumulation.These findings provide a roadmap for further optimizing the production of marbled meat through nutritional intervention in actual livestock breeding production.
基金supported by the National Natural Science Foundation of China(U2344201 and 42101316)the Natural Science Foundation of Hunan Province,China(2022JJ40866)the Outstanding Youth Project of Education Bureau of Hunan Province,China(20B613)。
文摘Understanding the spatial distributions and corresponding variation mechanisms of key soil nutrients in fragile karst ecosystems can assist in promoting sustainable development.However,due to the implementation of ecological restoration initiatives such as land-use conversions,novel changes in the spatial characteristics of soil nutrients remain unknown.To address this gap,we explored nutrient variations and the drivers of the variation in the 0–15 cm topsoil layer using a regional-scale sampling method in a typical karst area in northwest Guangxi Zhuang Autonomous Region,Southwest China.Descriptive statistics,geostatistics,and spatial analysis were used to assess the soil nutrient variability.The results indicated that soil organic carbon(SOC),total nitrogen(TN),total phosphorus(TP),and total potassium(TK)concentrations showed moderate variations,with coefficients of variance being 0.60,0.60,0.71,and 0.72,respectively.Moreover,they demonstrated positive spatial autocorrelations,with global Moran's indices being 0.68,0.77,0.64,and 0.68,respectively.However,local Moran's index values were low,indicating large spatial variations in soil nutrients.The best-fitting semi-variogram models for SOC,TN,TP,and TK concentrations were spherical,Gaussian,exponential,and exponential,respectively.According to the classification criteria of the Second National Soil Census in China,SOC and TN concentrations were relatively sufficient,with the proportions of rich and very rich levels being up to 90.9 and 96.0%,respectively.TP concentration was in the mediumdeficient level,with the areas of medium and deficient levels accounting for 33.7 and 30.1%of the total,respectively.TK concentration was deficient,with the cumulative area of extremely deficient,very deficient,and deficient levels accounting for 87.6%of the total area.Consequently,the terrestrial ecosystems in the study area were more vulnerable to soil P and K than soil N deficiencies.Furthermore,variance partitioning analysis of the influencing factors showed that,except for the interactions,the single effect of other soil properties accounted more for soil nutrient variations than spatial and environmental variables.These results will aid in the future management of terrestrial ecosystems.
基金supported by the Second Tibetan Plateau Scientific Expedition and Research Program(STEP)(No.2019QZKK0208)the National Natural Science Foundation of China(Nos.42171148 and 42330512)the Key R&D Project from the Science and Technology Department of Tibet(No.XZ202501ZY0030).
文摘Nitrogen(N)and phosphorus(P)are essential nutrients and can significantly impact primary productivity of the ecosystem causing water environmental problems.However,their cycling mechanisms are not well understood in alpine mountains with climate change.Hence,94 samples of river water were collected from 2018 to 2020 in the headwaters of the Shule River Basin to assess the nutrients spatiotemporal distribution and combined ap-proach of water quality index to assess water quality and potential sources.The findings depict that high nutrient concentrations were found to coincide with snowmelt and glacial meltwater and rainfall recharge periods,while total flux peaked from June to September due to increased runoff.Notably,total nitrogen(TN)concentrations were significantly higher near the town,primarily attributed to the replenishment of nitrate(NO_(3)^(‒)-N)from live-stock manure.The high total P(TP)was near the glacier,which was attributed to the transportation of glacial sediments into the river,and pH was another critical factor.N was the primary nutrient limiting factor for the growth of phytoplankton in river water.Although the migration and transport of nutrients have altered with climate change,river water quality is good in alpine mountains based on an overall evaluation.These findings contribute to enriching nutrient datasets and highlight the importance of water resource management and water quality assessment in sensitive and fragile alpine mountains.
基金Supported by the Laoshan Laboratory(No.LSKJ202204005)the Strategic Priority Research Program of the Chinese Academy of Sciences(No.XDA23050502)+2 种基金the State Key Program of National Natural Science Foundation of China(No.42130411)the International Science Partnership Program of the Chinese Academy of Sciences(Nos.121311KYSB20190029,133137KYSB20200002)the Key Deployment Project of Centre for Ocean Mega-Research of Science,Chinese Academy of Sciences(CAS)(No.COMS2019J03)。
文摘Nutrients play a crucial role in sustaining marine ecosystems and supporting mariculture,especially in seaweed aquaculture.Currently,seaweed farming,such as kelp cultivation,is entirely dependent on the natural supply of nutrients.Sanggou Bay in Shandong Peninsula,Yellow Sea,is renowned for its 60-year history of kelp cultivation;however,it is recently facing an increasing demand for nitrogen and phosphorus due to the expansion in aquaculture scale and production.There is no doubt that nutrient addition can enhance and sustain the production,but it is crucial to understand its effect on kelp growth under current nutrients condition and the potential ecological risks.Our in-field nutrient enrichment experiments show that nitrogen and phosphorus additions promoted the kelp biomass during the early growth stages,and have no adverse effects on phytoplankton or seawater nutrient levels throughout the experiment.From a long-term perspective,increasing nutrient supply appears to be an essential strategy for sustaining the aquaculture of kelp.
基金supported by the China Agriculture Research System(Grant No.CARS-23-D06)the Key Research and Development Program of Shaanxi Province(Grant Nos.2024NC2-GJHX-29 and 2024NC-ZDCYL-05-08)Shaanxi Agricultural Collaborative Innovation and Extension Alliance Project(Grant No.LMZD202202).
文摘Substrate and nutrient supply are essential for vegetable cultivation in greenhouse.The strategies for plant nutrient supply vary depending on the cultivation methods or substrate dosages employed.With the development of mechanization,wide-row spacing substrate cultivation became an optimize mode of the greenhouse cucumber cultivation,aligning with the trend of intelligent agriculture.To determine the optimal nutrient solution supply amount(NS)and supply frequency(SF)for promoting the integrated growth of cucumber under wide-row spacing substrate cultivation,we explored the effects of substrate supply amount(SS),NS,and SF on cucumber yield,quality,and element utilization efficiency.A five-level quadratic orthogonal rotation combination design with three experimental factors(NS,SF,and SS)was implemented for 23 coupling treatments over three growing seasons,including spring(2022S and 2023S)and autumn(2022A).The technique for order preference by similarity to ideal solution(TOPSIS)combining weights based on game theory was applied to construct cucumber comprehensive growth evaluation model.Single and two experimental factors analyses revealed significant effects of single factors and the coupling of NS-SS,NS-SF and SS-SF on the integrated growth of cucumber for all three growing seasons.For the NS-SF-SS combination,the optimal parameters for comprehensive cucumber growth were determined as follows:levels of^(-1).68 for NS,-0.7 for SF,and^(-1).682 for SS in 2022A;-0.43 for NS,-0.06 for SF,and 0.34 for SS in 2022S;0.3 for NS,-0.02 for SF,and 0.04 for SS in 2023S.Furthermore,for SS ranges of 2.00-3.01,3.01-4.50,4.50-5.99,5.99-7.00(L·plant^(-1)),the corresponding NS and SF intervals maximizing cucumber integrated growth in spring were:0.28-0.30(L·plant^(-1))and 6(times·d^(-1)),0.26-0.30(L·plant^(-1))and 6(times·d^(-1)),0.25-0.30(L·plant^(-1))and 6(times·d^(-1)),0.23-0.30(L·plant^(-1))and 6(times·d^(-1)),respectively.With the same SS,the corresponding NS and SF intervals that maximized cucumber integrated growth in autumn were:0.10(L·plant^(-1))and 8(times·d^(-1)),0.18(L·plant^(-1))and 7(times·d^(-1)),0.30(L·plant^(-1))and 6(times·d^(-1)),0.49(L·plant^(-1))and 5(times·d^(-1)),respectively.The results provide a theoretical basis for solution management,and further in-depth research on cucumber cultivation.
基金funded by Taif University,Saudi Arabia,Project No.(TU-DSPP-2025-30)The Science and Technology Fellowship Trust(SL No.39.00.0000.035.22.013.19.144)under the Ministry of Science and Technology of Bangladesh partially financed the current research。
文摘Improving crop productivity and soil fertility through the balanced application of inorganic and organic nutrient sources is a sustainable approach in modern agriculture.Char land soils,widely distributed in riverine Bangladesh,are generally low in organic matter status and deficient in necessary nutrient elements for crop production.Addressing this challenge,the present study was conducted to investigate the effects of various organic nutrient sources with inorganic fertilizers on crop yields,nutrient uptake,and soil fertility in farm(L1)and char land(L2)of Brahmaputra River in Mymensingh,Bangladesh from 2022(Y1)to 2023(Y2).For each location,eight treatments viz.T1(Control),T2[100%recommended fertilizer dose(RFD)],T3(75%RFD),T4(75%N from RFD 25%N from cow dung),T5(75%+N from RFD 25%N from poultry manure),T6(75%N from RFD 25%N from vermicompost),T7(75%N from++RFD 25%N from household compost)and T8(75%N from RFD 25%N from rice straw compost)were arranged in++a randomized complete block design with three replications using Wheat–Mungbean–T.Aman rice cropping pattern where three way interaction was considered for results.Treatment T5 performed the best in both years in both locations as it enhanced the yield components(p 0.05)and caused yield increment over control.The yield improvement in<Char land soils was higher than that in farm soils.For all three crops,treatment T5 consistently augmented the uptake of nitrogen,phosphorus,potassium,and sulphur by different parts of the crops and improved soil fertility properties such as organic matter status,cation exchange capacity,total nitrogen,available phosphorus,and sulphur as well as exchangeable potassium in both locations in both years.Cost and return analysis of different treatments for the whole cropping system showed that the highest marginal benefit-cost ratio(16.35 and 15.07)and gross return(about Tk 768,595/ha and 728,341/ha)were obtained from the T5 treatment in farm soils and Char land soils,respectively.Followed by poultry manure,vermicompost performed well in addition to mineral fertilizers for improving crop yield and soil fertility but its economic efficiency was less due to high input cost.These findings may be useful to the smallholder farmers in char areas,who could benefit from increased productivity,reduced reliance on chemical fertilizers,and improved soil health,contributing to the long-term sustainability of char land agriculture.
基金supported by the National Natural Science Foundation of China(NSFC)(No.42107299).
文摘To better understand the effects of ground-level ozone(O_(3))on nutrients and stoichiometry in different plant organs,urban tree species Celtis sinensis,Cyclocarya paliu-rus,Quercus acutissima,and Quercus nuttallii were sub-jected to a constant exposure to charcoal-filtered air(CF),nonfiltered air(NF),or NF+40,60,or 80 nmol O_(3)mol^(-1)(NF40,NF60,and NF80)starting early in the summer of the growing season.At the end of summer,net CO_(2)assimila-tion rate(A),stomatal conductance(gs),leaf mass per area(LMA),and/or leaf greenness(SPAD)either were not sig-nificantly affected by elevated O_(3)or were even higher in some cases during the summer compared with the CF or NF controls.LMA was significantly lower in autumn only after the highest O_(3)exposures.Compared to NF,NF40 caused a large increase in gs across species in late summer and more K and Mn in stems.At the end of the growing season,nutri-ent status and stoichiometric ratios in different organs were variously altered under O_(3)stress;many changes were large and often species-specific.Across O_(3)treatments,LMA was primarily associated with C and Mg levels in leaves and Ca levels in leaves and stems.NF40 enriched K,P,Fe,and Mn in stems,relative to NF,and NF60 enhanced Ca in leaves relative to CF and NF40.Moreover,NF resulted in a higher Ca/Mg ratio in leaves of Q.acutissima only,relative to the other O_(3)regimes.Interestingly,across species,O_(3)stress led to different nutrient modifications in different organs(stems+branches vs leaves).Thus,ambient and/or elevated O_(3)exposures can alter the dynamics and distribution of nutrients and disrupt stoichiometry in different organs in a species-specific manner.Changes in stoichiometry reflect an important defense mechanism in plants under O_(3),and O_(3)pollution adds more risk to ecological stoichiometries in urban areas.
文摘While many harmful algal blooms have been associated with increasing eutrophication, not all species respond similarly and the increasing challenge, especially for resource managers, is to determine which blooms are related to eutrophication and to understand why particular species proliferate under specific nutrient conditions. The overall goal of this brief review is to describe why nutrient loads are not changing in stoichiometric proportion to the "Redfield ratio", and why this has important consequences for algal growth. Many types of harmful algae appear to be able to thrive, and/or increase their production of toxins, when nutrient loads are not in proportion classically identified as Redfield ratios. Here we also describe some of the physiological mechanisms of different species to take up nutrients and to thrive under conditions of nutrient imbalance.
基金supported by the National Key Research and Development Program(No.2021YFD1300201)Jilin Provincial Department of Science and Technology Innovation Platform and Talent Special Project(No.20230508090RC).
文摘Background There is a growing focus on using various plant-derived agricultural by-products to increase the benefits of pig farming,but these feedstuffs are fibrous in nature.This study investigated the relationship between dietary fiber physicochemical properties and feedstuff fermentation characteristics and their effects on nutrient utilization,energy metabolism,and gut microbiota in growing pigs.Methods Thirty-six growing barrows(47.2±1.5 kg)were randomly allotted to 6 dietary treatments with 2 apparent viscosity levels and 3β-glucan-to-arabinoxylan ratios.In the experiment,nutrient utilization,energy metabolism,fecal microbial community,and production and absorption of short-chain fatty acid(SCFA)of pigs were investigated.In vitro digestion and fermentation models were used to compare the fermentation characteristics of feedstuffs and ileal digesta in the pig’s hindgut.Results The production dynamics of SCFA and dry matter corrected gas production of different feedstuffs during in vitro fermentation were different and closely related to the physical properties and chemical structure of the fiber.In animal experiments,increasing the dietary apparent viscosity and theβ-glucan-to-arabinoxylan ratios both increased the apparent ileal digestibility(AID),apparent total tract digestibility(ATTD),and hindgut digestibility of fiber components while decreasing the AID and ATTD of dry matter and organic matter(P<0.05).In addition,increasing dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios both increased gas exchange,heat production,and protein oxidation,and decreased energy deposition(P<0.05).The dietary apparent viscosity andβ-glucanto-arabinoxylan ratios had linear interaction effects on the digestible energy,metabolizable energy,retained energy(RE),and net energy(NE)of the diets(P<0.05).At the same time,the increase of dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios both increased SCFA production and absorption(P<0.05).Increasing the dietary apparent viscosity andβ-glucan-to-arabinoxylan ratios increased the diversity and abundance of bacteria(P<0.05)and the relative abundance of beneficial bacteria.Furthermore,increasing the dietaryβ-glucan-to-arabinoxylan ratios led to a linear increase in SCFA production during the in vitro fermentation of ileal digesta(P<0.001).Finally,the prediction equations for RE and NE were established.Conclusion Dietary fiber physicochemical properties alter dietary fermentation patterns and regulate nutrient utilization,energy metabolism,and pig gut microbiota composition and metabolites.
文摘Diel investigations of water environments are one means to holistically understand the dynamics and functional roles of phytoplankton,bacteria and viruses in these ecosystems.They have the potential to substantially impact carbon(C),nitrogen(N)and phosphorus(P)biogeochemistry through their respective roles.This study characterizes the phytoplankton,bacteria and virus communities and the elemental composition of various C,N and P nutrients flow over three diel cycles in tropical urban lake.Our results show that ratios of C:N:P fluctuated strongly from the lack of dissolved organic phosphorus(DOP)and PO_(4).Specifically,green algae peaked during day time and exudate dissolved organic matter(DOM)that strongly modulate dissolved organic carbon(DOC):DOP ratio to diel DOP limitation.Multiple linear regression and Stella modelling emphasize the roles of viruses together with Synechococcus as important nutrient recyclers of NH_(4)and PO_(4)in nutrients-limited waters.Respective normalised surface PO_(4)and combined surface and bottom NH_(4)concentration selected both viruses and Synechococcus as important drivers.Process model of N and P biogeochemical cycles can achieve 69%and 57%similar to observed concentration of NH_(4)and PO_(4),respectively.A short latent period of 9 hr was calculated,in addition to the calibrated high infectivity of viruses to Synechococcus.Taken together,the rapid turn-over between Synechococcus and viruses has biogeochemical significance,where the rapid recycling of essential nutrients allows for shortcuts in the N and P cycle,supporting a wide range of microbes.
基金supported by the National Key Research and Development Program of China(2023YFD2100205)the Fujian Province Science and Technology Plan Project,China(2023N3008).
文摘With the rapid growth of the global population and the increasing demand for healthier diets,improving the nutrient utilization efficiency of staple food crops has become a critical scientific and industrial chal-lenge,prompting innovation in food processing technologies.This review introduces first the common nutritional challenges in the processing of staple food crops,followed by the comprehensive examination of research aiming to enhance the nutritional quality of staple food crop-based foods through innovative processing technologies,including microwave(MW),pulsed electric field(PEF),ultrasound,modern fer-mentation technology,and enzyme technology.Additionally,soybean processing is used as an example to underscore the importance of integrating innovative processing technologies for optimizing nutrient utilization in staple food crops.Although these innovative processing technologies have demonstrated a significant potential to improve nutrient utilization efficiency and enhance the overall nutritional pro-file of staple food crop-based food products,their current limitations must be acknowledged and addressed in future research.Fortunately,advancements in science and technology will facilitate pro-gress in food processing,enabling both the improvement of existing techniques as well as the develop-ment of entirely novel methodologies.This work aims to enhance the understanding of food practitioners on the way processing technologies may optimize nutrient utilization,thereby fostering innovation in food processing research and synergistic multi-technological strategies,ultimately providing valuable references to address global food security challenges.
文摘To determine the effects of preharvest arginine spraying on the nutritional level of broccoli and the mechanism of action of arginine in improving the storage quality of broccoli,arginine spraying(5 mmol/L)was conducted at 0,1,3,and 5 days before harvest.The appearance,respiration rate,mass-loss rate,electrolyte leakage,glucosinolate,ascorbic acid,total phenol,total flavonoid,total sugar and sucrose contents,and sucrose phosphate synthase(SPS),invertase(INV),sucrose synthase synthesis(SSS)and cleavage(SSC)activities of broccoli samples were observed after 0,2,4,6,8,and 10 days of storage.The results showed that spraying arginine at 5 days preharvest(5-ARG)helped to inhibit broccoli respiration during storage,delay electrolyte leakage,and maintain broccoli color.Furthermore,during the growth stage,total sugar accumulation was higher in the 5-ARG group.In addition,during the storage period,sucrose synthesis was accelerated,while sucrose cleavage was inhibited,resulting in more sucrose retention in postharvest broccoli.In conclusion,5-ARG resulted in the accumulation of more nutrients during the growth process and effectively delayed the quality decline during storage,thereby prolonging the shelf life of broccoli.Therefore,this study provides a theoretical basis for improving postharvest storage characteristics of broccoli through preharvest treatments.
基金Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.
文摘The concentrations of the foliar and surface soil nutrients and the variation with species and stand age were studied inPinus spp. plantations in Zhanggutai area, northeast China. The results showed that the total N, total P and C: N ratio of the soil inP. sylvestris var.mongolica stands were significantly higher in comparison with those inP. tabulaeformis andP. densiflora stands. ForP. sylvestris var.mongolica, the foliar P concentration appeared to decrease with age, and the foliar N and K concentrations did not show a consistent change with age. As for the different tree species of the similar age, the foliar N and P concentrations were significantly different (p<0.05), being withP. sylvestris var.mongolica>P. densiflora>P. tabulaeformis. The foliar N: P ratio ofP. densiflora significantly was higher thanP. sylvestris var.mongolica andP. tabulaeformis, while the foliar K was no obvious difference between the three tree species. There were significant correlation (p<0.05) between soil total N and P, soil organic matter and total P, foliar N and P, but it did not show significant correlations between soil and foliar nutrient concentrations, which might attribute to the excessive litter raking, overgrazing and low soil moisture in this area. Based on the foliar N: P ratio, we introduced a combination threshold index of N: P ratio with their absolute foliar nutrient concentrations to determine the possible limiting nutrient. According to the critical N: P ratio and their absolute foliar N, P concentrations, theP. sylvestris var.mongolica stands showed a decreased N limitation degree with age, theP. densiflora stands showed unlimited by N and P in the whole, and theP. tabulaeformis stands showed co-limited by N and P. No significant difference in soil nutrient concentrations of the surface soils was found between 45, 29, 20-yr-oldPinus sylvestris var.mongolica plantation stands. Keywords coniferous trees - foliar nutrient concentration - limiting nutrients - N - P ratio - Zhanggutai sandy land CLC number S718.55 Document code A Article ID 1007-662X(2004)01-0011-08 Foundation item: This research was supported by Key Knowledge Innovation Project (KZCX3-SW-418) of Chinese Academy of Sciences.Biography: CHEN Guang-sheng (1978-), male, master candidate in Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, P. R. ChinaResponsible editor: Song Funan
基金The study was supported by PPI/PPIC China Program (No. HB-19) and Wetland Laboratory Opening Foundation of Hubei Province (No. HNKFJ20021301).
文摘A study was conducted to evaluate the soil nutrient status of poplar plantation by Soil Nutrient Systematic Approach (SNSA) in Jianghan Plain, Hubei Province, China. Soil physiochemical properties were analyzed in laboratory through collection soil samples of study site. Ten treatments of application different fertilizers were designed such as CK, optimum treatment (N, P, K, Zn), N(P, K, Zn), P(N, K, Zn), K(N, P, Zn), +Mg(N, P, K, Zn, Mg), Zn (N,P,K), +2P(N, 2P, K, Zn), +2K(N, P, 2K, Zn), and 2N+2P+2K(2N, 2P, 2K, Zn) for field experiment to test the effect on tree height, diameter (DBH) growth, and dry weight of poplar. The results showed that there was no significant difference in tree heights between treatments with different fertilizers, diameter growth of poplar trees in treatments of lack of N and Zn was significantly slower than that of trees in optimum treatment, and dry weight of poplar dropped significantly for treatment of CK as well as treatments without application N and Zn. It is concluded that N and Zn were main limiting factor for poplar growth. Results from laboratory analysis and field experiment were uniform per-fectly, which proved that SNSA was reliable in evaluating soil nutrient status of poplar plantation.
文摘A field trial to evaluate the growth and nutrient uptake of cocoa intercropped with coconut was conducted in Cocoa Research Institute of Nigeria(CRIN)Headquarters,Idi-Ayunre,Ibadan,Oyo State between 2019 and 2022.There were four treatments comprising of cocoa sole,cocoa/plantain,cocoa/coconut and cocoa/coconut/plantain intercrop.The experimental design was Randomized Complete Block(RCB)replicated three times.Result obtained showed that cocoa/plantain was significantly higher(p<0.05)than all other treatments in all parameters considered and closely followed by cocoa/coconut,then cocoa sole with cocoa/plantain/coconut giving the least performance.The same trend was observed in the nutrient uptake.It was also observed in the trial that the two crops:cocoa and coconut did not show any deleterious effect on their growth when intercropped together.This showed that the two crops are compatible when grown on the same piece of land.Therefore,cocoa/coconut intercrop could be recommended to cocoa farmers in south west Nigeria to increase their revenue generation when coconut starts bearing fruit instead of sole planting of cocoa.
文摘Selenium(Se)is a nutrient that is considered beneficial for plants,because its improvement in growth,yield and quality helps plants to mitigate stress.The objective of this research was to evaluate the application of sodium selenite(Na2SeO3),nanoparticles(SeNPs)and microparticles(SeMPs)of Se in cucumber seedlings,via two experiments:one with seed priming and the other with foliar application of Sematerials.The doses used were:0,0.1,0.5,1.0,1.5 and 3.0 mg⋅L^(−1),for each form of Se and for each form of application.Treatment 0 consisted of the application of distilled water,which was used as a control.The results indicated that the SeMPs treatment at 3.0 mg⋅L^(−1)for seed priming had the greatest effect on stem diameter and leaf area.Foliar application of SeMPs at 1.5 mg⋅L^(−1)was the most effective at increasing the leaf area.In terms of fresh and dry biomass(aerial,root and total)for seed priming,all the treatments were superior to the control,and SeMPs at 1.5 and 3.0 mg⋅L^(−1)caused the greatest effects.With foliar application,fresh root biomass improved to a greater extent with the SeMPs treatment at 3.0 mg⋅L^(−1),and dry biomass(aerial,root and total)increased with the SeMPs at 1.0 and 3.0 mg⋅L^(−1).With respect to the photosynthetic pigments,proteins,phenols and minerals,the Se treatments,both for seed priming and foliar application,caused increases and decreases;however,reduced glutathione(GSH)increased with treatments in both forms of application.The Se concentration in the seedlings increased as the dose of Se material increased,and greater accumulation was achieved with foliar application of SeNPs and SeMPs.The results indicate that the use of Se materials is recommended,mainly the use of SeMPs,which improved the variables studied.This opens new opportunities for further studies with SeMPs,as little information is available on their application in agricultural crops.
基金supported by the Long-term Research Programme“Sustainable Forestry and Global Changes”,conducted by the Lithuanian Research Centre for Agriculture and Forestry(LAMMC).
文摘Afforestation on formerly cultivated or aban-doned agricultural land is a common strategy to increase forest areas and enhance carbon sequestration.Deep soil ploughing before afforestation improves soil conditions,facilitating tree growth and carbon storage.This study assessed the growth and biomass parameters of Pinus sylves-tris in 10-and 20 years old plantations established on deeply ploughed and non-ploughed soils in Lithuania.Biomass allocation and carbon and nutrient concentrations including N,P,K,Ca and Mg were analysed in aboveground biomass components.Deep ploughing in the 10 years old stands negatively impacted vertical growth and stem development but did not significantly affect overall biomass accumula-tion.In contrast,in the 20 years old stands,deep plough-ing resulted in taller trees with larger diameters and higher biomass accumulation compared to non-ploughed sites.Biomass distribution within tree canopies varied between ploughed and non-ploughed sites,indicating diverse effects of deep ploughing.Carbon and nutrient concentrations in biomass components showed site-specific variations,with deep ploughing influencing carbon concentrations in needles and stem bark.Overall,deep ploughing showed potential for enhancing tree growth and biomass accumulation,with implications for carbon sequestration in forest ecosystems.These findings help us understand the impact of an alternative soil management practice,deep ploughing,on forest development and carbon dynamics.
基金Natural Science Foundation of Heilongjiang Province(YQ2022C014)National Natural Science Foundation of China(32302768).
文摘Background Progressive oxidative stress easily occurs as a result of a gradual increase in the intensity of maternal metabolism due to rapid foetal development and increased intensity of lactation.However,studies on the effects of processive oxidative stress on nutrient transport in the placenta have received little attention.The present study was conducted on sows at 85 days of gestation to study the effects of pterostilbene(PTE)on maternal oxidative stress status and placental nutrient transport.Results PTE increased the antioxidant capacity and immunoglobulin content in mothers’blood and milk,reduced the level of inflammatory factors,and improved the nutrient content of milk.PTE also reduced sow backfat loss and the number of weak sons,and increased piglet weaning weight and total weaning litter weight.We subsequently found that PTE enhanced placental glucose and fatty acid transport and further affected glycolipid metabolism by increasing the expression of LAL,PYGM,and Gbe-1,which activated the PI3K phosphorylation pathway.Moreover,PTE addition altered the relative abundance of the Firmicutes,Proteobacteria,Parabacillus,and Bacteroidetes-like RF16 groups in sow faeces.PTE increased the levels of acetate,propionate,butyrate and isovalerate in the faeces.Conclusions These findings reveal that the addition of PTE during pregnancy and lactation mitigates the effects of processive oxidative stress on offspring development by altering maternal microbial and placental nutrient trans-port capacity.
基金supported by the National Natural Science Foundation of China (No.42007026)the Medical Innovation and Development Project of Lanzhou University (lzuyxcx-2022-172)
文摘The degradation of animal carcasses can lead to rapid waste release(e.g.,pathogenic bacteria,viruses,prions,or parasites)and also result in nutrient accumulation in the surrounding environment.However,how viral profile responds and influences nutrient pool(carbon(C),nitrogen(N),phosphorus(P)and sulfur(S))in polluted water caused by animal carcass decomposition had not been explored.Here,we combined metagenomic analysis,16S rRNA gene sequencing and water physicochemical assessment to explore the response of viral communities under different temperatures(23℃,26℃,29℃,32℃,and 35℃)in water polluted by cadaver,as well as compare the contribution of viral/bacterial communities on water nutrient pool.We found that a total of 15,240 viral species were classified and mainly consisted of Siphoviridae.Both temperature and carrion reduced the viral diversity and abundance.Only a small portion of the viruses(∼8.8%)had significant negative correlations with temperature,while most were not sensitive.Our results revealed that the viruses had lager contribution on nutrient pool than bacteria.Besides,viral-related functional genes involved in C,N,P and S cycling.These functional genes declined during carcass decomposition and covered part of the central nutrient cycle metabolism(including carbon sugar transformation,denitrification,P mineralization and extracelluar sulfate transfer,etc.).Our result implies that human regulation of virus communities may be more important than bacterial communities in regulating and managing polluted water quality and nutrition.
文摘Tropical coastal ecosystems,vital habitats for economically significant marine species,face increasing threats from eutrophication driven by human-induced nutrient pollution.This study investigated the metabolomic profile of Anadara granosa,a marine bivalve,to identify metabolites indicative of eutrophic conditions.Conducted in Cirebon Bay,Indonesia,the research spanned five locations reflecting varying eutrophication levels.Bottom-layer water samples were analyzed for pH,dissolved oxygen,salinity,water transparency,and nutrient concentrations(phosphate,nitrate,ammonia).Proton-Nuclear Magnetic Resonance(Proton-NMR)metabolomics of A.granosa revealed metabolite variations linked to water quality.Eutrophic conditions,prevalent in the bay’s northern side,were characterized by inorganic nitrogen levels above 0.2 mg L^(-1) and phosphate exceeding 0.024 mg L^(-1).These were linked to urban,aquaculture,and agricultural runoff,driving increased phytoplankton biomass and reduced salinity variability.Metabolomic analysis identified inosine and dimethylglycine as potential biomarkers.Elevated inosine levels in eutrophic environments suggest increased cellular activity due to greater food availability,while higher dimethylglycine concentrations detected in low-nutrient as its role in osmotic regulation.Both metabolites correlated with ammonia concentrations(P<0.05,R^(2)=0.631),a key contributor to eutrophication.These findings highlight inosine and dimethylglycine as effective indicators of nutrient enrichment,providing insights into eutrophication’s impact on coastal ecosystems and A.granosa’s physiological responses.
