Natural grazing land plays a crucial role in extensive ruminant livestock systems,especially in semi-arid tropical regions such as East Nusa Tenggara(ENT),Indonesia.The availability and quality of forage during the dr...Natural grazing land plays a crucial role in extensive ruminant livestock systems,especially in semi-arid tropical regions such as East Nusa Tenggara(ENT),Indonesia.The availability and quality of forage during the dry season present significant challenges.This study aimed to identify variations in grass species composition and fluctuations in forage nutritional content in natural grazing lands of ENT during the dry season(July–October 2024).Sampling was conducted in four sub-districts:two representing lowland zones and two representing highland zones.In each sub-district,four grazing fields were selected,and ten plots were sampled per grazing field,totaling 160 sampling plots.Species identification and nutrient analysis included crude protein,crude fiber,energy content,and proteinenergy ratio.Statistical analyses using ANOVA and Tukey’s multiple comparison test were performed to evaluate significant differences in nutritional parameters across months and zones.Dominant species identified were Themeda arguens,Heteropogon contortus,Brachiaria decumbens,Ischaemum timorense,Cynodon dactylon,and Pennisetum clandestinum.Results showed significant monthly fluctuations in crude protein and fiber contents(p<0.05),with protein levels decreasing from July(9.31±2.66%)to October(7.53±3.10%).Energy content and protein-energy ratio also varied significantly across the dry season.A monthly shift in dominant grass species composition was observed,influenced by environmental conditions and species adaptability.The protein-energy ratio of forage remained below optimal levels throughout the dry season,potentially limiting livestock productivity.These findings provide important scientific insights for developing climate-resilient feeding strategies and support policy formulation for sustainable tropical livestock farming in semi-arid regions.展开更多
From the existing literature and data from museum specimens an overview is presented of all currently known terrestrial and semi-aquatic snakes of the Lesser Sunda Islands, in the Wallacean area of Indonesia. In total...From the existing literature and data from museum specimens an overview is presented of all currently known terrestrial and semi-aquatic snakes of the Lesser Sunda Islands, in the Wallacean area of Indonesia. In total, twenty-nine species are known to inhabit the area. Of these eight are endemic to the area： Boiga hoeseli, Coelognathus subradiatus, Dendrelaphis inornatus, Stegonotus florensis, Cylindrophis opisthorhodus, Broghammerus timoriensis, Liasis mackloti and Typhlops schmutzi. Insular endemism is only found at the subspecific level, including Liasis mackloti dunni （Wetar）, Liasis mackloti savuensis （Sawu）, Ramphotyphlops polygrammicus brongersmai （Sumba）, Ramphotyphlops polygrammicus elberti （Lombok） and Ramphotyphlops polygrammicus florensis （Flores）. Such endemism may be due to the relatively young geological age of the Lesser Sunda Islands and that the snake fauna is still underestimated. Taxonomy of the genus Cylindrophis, the species Coelognathus subradiatus, Dendrelaphis inornatus, Cryptelytrops insularis, and the five subspecies of Ramphotyphlops polygrammicus need to be reviewed. Ecological studies are urgently required to establish if the species Broghammerus timoriensis and Liasis mackloti savuensis are endangered and which conservation measures should be taken. Telah dilakukan pengamatan ulang dari kepustakaan yang ada, data dari databank Western Australian Museum, maupun koleksi museum. Hasil tersebut dirangkum dalam suam tinjauan mengenai semua jenis ular yang hidup di darat maupun di air-tawar dari daerah Nusa Tenggara sebagai bagian dari daerah Wallacea. Jumlah jenis yang dapat dipastikan berjumlah dua puluh sembilan jenis, dan delapan di antaranya merupakan jenis yang endemik, yaitu Boiga hoeseli, Coelognathus subradiatus, Dendrelaphis inornatus, Stegonotus florensis, Cylindrophis opisthorhodus, Broghammerus timoriensis, Liasis mackloti dan Typhlops schmutzi. Jenis endemic dalam daerah pulau hanya meliputi anak jenis： Liasis mackloti dunni （Wetar）, Liasis mackloti savuensis （Sawu）, RamphotyphIops polygrammicus brongersmai （Sumba）, Ramphotyphlops polygrammicus elberti （Lombok） dan Ramphotyphlops polygrammicus florensis （Flores）. Hal ini diperkirakan merupakan konsekuensi langsung dari sejarah geologi yang relatif singkat namun dapat juga disebabkan karena kekayaan jenis ular kurang dipelajari dengan lebih seksama. Kedudukan sistematik dari Coelognathus subradiatus, Dendrelaphis inornatus, Cylindrophis boulengeri, C. opisthorhodus, dan ke-lima subspecies Ramphotyphlops polygrammicus serta Cryptelytrops insularis perlu ditinjau kembali. Jenis-jenis yang diperkirakan terancam dan perlu perlindungan adalah jenis-jenis piton Broghammerus timoriensis dan Liasis maekloti savuensis. Penelitian ekologi perlu segara dilakukan untuk menentukan apakah jenis-jenis tersebut perlu perlindungan, dan juga tindakan konservasi yang mana bisa diambil.展开更多
Microcystinase(MlrA)is a key endopeptidase that catalyzes microcystin degradation without generating harmful byproduct.However,the application of MlrA in the field is primarily impeded by its limited productivity and ...Microcystinase(MlrA)is a key endopeptidase that catalyzes microcystin degradation without generating harmful byproduct.However,the application of MlrA in the field is primarily impeded by its limited productivity and short lifespan.Therefore,the MlrA's function was studied by modelling its structure,which subsequently increased its heterologous expression and high-temperature stability.Results demonstrate that after the irregular sequence at the C-terminus of MlrA was removed,enzyme solubility was significantly decreased.In addition,three fusion tags,namely maltose-binding protein,glutathione S-transferase(GST),and N-utilization substance A(NusA)were used to enhance the overexpression of soluble recombinant MlrA,among which NusA-MlrA exhibited the highest solubility.Moreover,NusA-MlrA was active in pH 4-10 at 20-80℃;even at 80℃,approximately 35.8%of fusion protein remained active.NusA-MlrA retained 89%of MlrA's activity even after 7 d of storage at 50℃;and on day 7,the protein retained>90%of its activity at pH 7.Finally,a stable,soluble,and long-lasting heterologous MlrA was successfully constructed that could eliminate microcystins in Escherichia coli C43(DE3).This study enriched the comprehension of MlrA's structure and enzymatic properties,by particularly addressing the endopeptidase's low expression and short lifespan,which improved its suitability for future applications.展开更多
Seaweed has the ability to use carbon from the environment through photosynthesis to produce biomass. The aim of this study is to estimate carbon sequestration by seaweed aquaculture as a strategy for climate change m...Seaweed has the ability to use carbon from the environment through photosynthesis to produce biomass. The aim of this study is to estimate carbon sequestration by seaweed aquaculture as a strategy for climate change mitigation. The study was undertaken at Gerupuk Bay, Lombok Island, West Nusa Tenggara Province, Indonesia. Four seaweed variants, such as Kappaphycus alvarezii var. Tambalang and Maumere, K. striatum and Eucheuma denticulatum, were cultivated with long-line system for three cultivation periods, starting from July to November, 2013. Each cultivation period was taken about 45 days. Parameters including weight increasement and carbon content of seaweeds were measured every 15 days of culture for each cultivation period in order to calculate carbon sequestration rate. The results showed that E. denticulatum had the highest carbon sequestration rate and significantly different （P 〈 0.05） compared with other variants for every cultivation period. Different seaweed variants have different capacity on carbon sequestration. Optimal utilization of the potential area for seaweed aquaculture could reduce a great quantity of CO2 from the atmosphere and help to mitigate global climate change process.展开更多
Species diversity of the genus Theloderma has been extensively studied in recent decades,with the majority of newly described species hailing from the Indochina region.We describe a new species of mediumsized Theloder...Species diversity of the genus Theloderma has been extensively studied in recent decades,with the majority of newly described species hailing from the Indochina region.We describe a new species of mediumsized Theloderma based on molecular and morphological evidence from lowland forest in the southern part of Central Java Province,Indonesia,that is highly similar to and considered cryptic within the T.horridum group.A phylogenetic approach shows that this population is a distinct genetic lineage and can be distinguished from its congeners by the absence of vomerine teeth,moderate irregular blunt warts and studded with calcified asperities on the dorsal skin,a distinct color pattern,and a distinct denticle formula in the tadpole.Although the range of the new species is known only from Central Java,it cannot be ruled out that it may extends into adjacent areas,such as other parts of Java and Sumatra.展开更多
基金supported by the Ministry of Education,Culture,Research,and Technology of the Republic of Indonesia through the Directorate of Research,Technology,and Community Service under the Primary Contract Number:073/E5/PG.02.00.PL/2024 and the Secondary Contract Number:432/UN15.22/SP2H/PL/2024.
文摘Natural grazing land plays a crucial role in extensive ruminant livestock systems,especially in semi-arid tropical regions such as East Nusa Tenggara(ENT),Indonesia.The availability and quality of forage during the dry season present significant challenges.This study aimed to identify variations in grass species composition and fluctuations in forage nutritional content in natural grazing lands of ENT during the dry season(July–October 2024).Sampling was conducted in four sub-districts:two representing lowland zones and two representing highland zones.In each sub-district,four grazing fields were selected,and ten plots were sampled per grazing field,totaling 160 sampling plots.Species identification and nutrient analysis included crude protein,crude fiber,energy content,and proteinenergy ratio.Statistical analyses using ANOVA and Tukey’s multiple comparison test were performed to evaluate significant differences in nutritional parameters across months and zones.Dominant species identified were Themeda arguens,Heteropogon contortus,Brachiaria decumbens,Ischaemum timorense,Cynodon dactylon,and Pennisetum clandestinum.Results showed significant monthly fluctuations in crude protein and fiber contents(p<0.05),with protein levels decreasing from July(9.31±2.66%)to October(7.53±3.10%).Energy content and protein-energy ratio also varied significantly across the dry season.A monthly shift in dominant grass species composition was observed,influenced by environmental conditions and species adaptability.The protein-energy ratio of forage remained below optimal levels throughout the dry season,potentially limiting livestock productivity.These findings provide important scientific insights for developing climate-resilient feeding strategies and support policy formulation for sustainable tropical livestock farming in semi-arid regions.
文摘From the existing literature and data from museum specimens an overview is presented of all currently known terrestrial and semi-aquatic snakes of the Lesser Sunda Islands, in the Wallacean area of Indonesia. In total, twenty-nine species are known to inhabit the area. Of these eight are endemic to the area： Boiga hoeseli, Coelognathus subradiatus, Dendrelaphis inornatus, Stegonotus florensis, Cylindrophis opisthorhodus, Broghammerus timoriensis, Liasis mackloti and Typhlops schmutzi. Insular endemism is only found at the subspecific level, including Liasis mackloti dunni （Wetar）, Liasis mackloti savuensis （Sawu）, Ramphotyphlops polygrammicus brongersmai （Sumba）, Ramphotyphlops polygrammicus elberti （Lombok） and Ramphotyphlops polygrammicus florensis （Flores）. Such endemism may be due to the relatively young geological age of the Lesser Sunda Islands and that the snake fauna is still underestimated. Taxonomy of the genus Cylindrophis, the species Coelognathus subradiatus, Dendrelaphis inornatus, Cryptelytrops insularis, and the five subspecies of Ramphotyphlops polygrammicus need to be reviewed. Ecological studies are urgently required to establish if the species Broghammerus timoriensis and Liasis mackloti savuensis are endangered and which conservation measures should be taken. Telah dilakukan pengamatan ulang dari kepustakaan yang ada, data dari databank Western Australian Museum, maupun koleksi museum. Hasil tersebut dirangkum dalam suam tinjauan mengenai semua jenis ular yang hidup di darat maupun di air-tawar dari daerah Nusa Tenggara sebagai bagian dari daerah Wallacea. Jumlah jenis yang dapat dipastikan berjumlah dua puluh sembilan jenis, dan delapan di antaranya merupakan jenis yang endemik, yaitu Boiga hoeseli, Coelognathus subradiatus, Dendrelaphis inornatus, Stegonotus florensis, Cylindrophis opisthorhodus, Broghammerus timoriensis, Liasis mackloti dan Typhlops schmutzi. Jenis endemic dalam daerah pulau hanya meliputi anak jenis： Liasis mackloti dunni （Wetar）, Liasis mackloti savuensis （Sawu）, RamphotyphIops polygrammicus brongersmai （Sumba）, Ramphotyphlops polygrammicus elberti （Lombok） dan Ramphotyphlops polygrammicus florensis （Flores）. Hal ini diperkirakan merupakan konsekuensi langsung dari sejarah geologi yang relatif singkat namun dapat juga disebabkan karena kekayaan jenis ular kurang dipelajari dengan lebih seksama. Kedudukan sistematik dari Coelognathus subradiatus, Dendrelaphis inornatus, Cylindrophis boulengeri, C. opisthorhodus, dan ke-lima subspecies Ramphotyphlops polygrammicus serta Cryptelytrops insularis perlu ditinjau kembali. Jenis-jenis yang diperkirakan terancam dan perlu perlindungan adalah jenis-jenis piton Broghammerus timoriensis dan Liasis maekloti savuensis. Penelitian ekologi perlu segara dilakukan untuk menentukan apakah jenis-jenis tersebut perlu perlindungan, dan juga tindakan konservasi yang mana bisa diambil.
基金Supported by the National Key R&D Program of China(No.2018YFA0903100)the National Natural Science Foundation of China(No.32071601)。
文摘Microcystinase(MlrA)is a key endopeptidase that catalyzes microcystin degradation without generating harmful byproduct.However,the application of MlrA in the field is primarily impeded by its limited productivity and short lifespan.Therefore,the MlrA's function was studied by modelling its structure,which subsequently increased its heterologous expression and high-temperature stability.Results demonstrate that after the irregular sequence at the C-terminus of MlrA was removed,enzyme solubility was significantly decreased.In addition,three fusion tags,namely maltose-binding protein,glutathione S-transferase(GST),and N-utilization substance A(NusA)were used to enhance the overexpression of soluble recombinant MlrA,among which NusA-MlrA exhibited the highest solubility.Moreover,NusA-MlrA was active in pH 4-10 at 20-80℃;even at 80℃,approximately 35.8%of fusion protein remained active.NusA-MlrA retained 89%of MlrA's activity even after 7 d of storage at 50℃;and on day 7,the protein retained>90%of its activity at pH 7.Finally,a stable,soluble,and long-lasting heterologous MlrA was successfully constructed that could eliminate microcystins in Escherichia coli C43(DE3).This study enriched the comprehension of MlrA's structure and enzymatic properties,by particularly addressing the endopeptidase's low expression and short lifespan,which improved its suitability for future applications.
文摘Seaweed has the ability to use carbon from the environment through photosynthesis to produce biomass. The aim of this study is to estimate carbon sequestration by seaweed aquaculture as a strategy for climate change mitigation. The study was undertaken at Gerupuk Bay, Lombok Island, West Nusa Tenggara Province, Indonesia. Four seaweed variants, such as Kappaphycus alvarezii var. Tambalang and Maumere, K. striatum and Eucheuma denticulatum, were cultivated with long-line system for three cultivation periods, starting from July to November, 2013. Each cultivation period was taken about 45 days. Parameters including weight increasement and carbon content of seaweeds were measured every 15 days of culture for each cultivation period in order to calculate carbon sequestration rate. The results showed that E. denticulatum had the highest carbon sequestration rate and significantly different （P 〈 0.05） compared with other variants for every cultivation period. Different seaweed variants have different capacity on carbon sequestration. Optimal utilization of the potential area for seaweed aquaculture could reduce a great quantity of CO2 from the atmosphere and help to mitigate global climate change process.
基金Direktorat Riset dan Pengabdian Masyarakat,Direktorat Jenderal Penguatan Riset dan Pengembangan Kementerian Riset,Teknologi,dan Pendidikan Tinggi Republik Indonesia(grant number 437.43/UN10.C10/PN/2020)provided financial support to NK for this work。
文摘Species diversity of the genus Theloderma has been extensively studied in recent decades,with the majority of newly described species hailing from the Indochina region.We describe a new species of mediumsized Theloderma based on molecular and morphological evidence from lowland forest in the southern part of Central Java Province,Indonesia,that is highly similar to and considered cryptic within the T.horridum group.A phylogenetic approach shows that this population is a distinct genetic lineage and can be distinguished from its congeners by the absence of vomerine teeth,moderate irregular blunt warts and studded with calcified asperities on the dorsal skin,a distinct color pattern,and a distinct denticle formula in the tadpole.Although the range of the new species is known only from Central Java,it cannot be ruled out that it may extends into adjacent areas,such as other parts of Java and Sumatra.
