A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was iden...A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was identified in a BC3F8 population derived from a cross of Nipponbare as the recipient with Xihui 18 as the donor parent. Z1364 carried three substitution segments distributed on chromosomes 1, 6, and 8. The mean substitution length was 1.19 Mb. Of 17 QTL identified on the substitution segments, qSP1 for spikelets per panicle, qSSD1 for seed-set density, and qNSB1 for number of secondary branches explained respectively 57.34%, 87.7%, and 49.44% of the corresponding phenotypic variance and were all linked to RM6777. Chi-square analysis showed that the increased kernel number in Z1364 was inherited recessively by a single gene. By fine mapping, qSP1 was delimited to a 50-kb region on the short arm of chromosome 1. Based on DNA sequence, a previously uncharacterized rice homolog of Arabidopsis thaliana AT4G32551 was identified as a candidate gene for qSP1 in which mutation increases the number of spikelets and kernels in Z1364. qSP1 was expressed in all tissues, but particularly in 1-cm panicles. The expression levels of OsMADS22, GN1A, and DST were upregulated and those of LAX2, GNP1, and GHD7 were downregulated in Nipponbare. These results provide a foundation for functional research on qSP1.展开更多
Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy plant.Transferring D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is att...Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy plant.Transferring D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is attractive in improving its economically important traits.Here,a hexaploid triticale 6D(6A)substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization(GISH),fluorescence in situ hybridization(FISH),and molecular markers.The GISH analysis showed that Lin 456 is a hexaploid triticalewith 14 rye(Secale cereale)chromosomes and 28 wheat chromosomes,whereas non-denaturing fluorescence in situ hybridization(ND-FISH)and molecular marker analysis revealed that it is a 6D(6A)substitution line.In contrast to previous studies,the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin 456.The wheat chromosome 6D was associatedwith increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three environments.The thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three environments.We propose that the hexaploid triticale 6D(6A)substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.展开更多
Following NCI design, the developmental genetic behavior of tiller number (TN) in three-line indica hybrid rice was studied using additive-dominance developmental genetic models and the corresponding statistical metho...Following NCI design, the developmental genetic behavior of tiller number (TN) in three-line indica hybrid rice was studied using additive-dominance developmental genetic models and the corresponding statistical methods. The results showed that dominance effects were predominant for TN. The expression of those additive effects were affected by environment and genotype interaction, but the expression of dominance effects were not affected. Heterosis was the strongest in the middle developmental periods of TN. Additive effects and dominance effects were selectively expressed throughout in the entire tillering developmental stage. Analysis of genetic correlation between TN at different stages and the productive panicles indicated that a close correlation appeared earlier in the populations with higher heterosis than in those with less heterosis. Utilization of heterosis at the middle tillering stage might enhance the final biomass but reduce the percentage of productive panicles.展开更多
Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand ＂static＂ and ＂dynamic＂ informati...Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand ＂static＂ and ＂dynamic＂ information of the QTLs for tillers in rice. This work was the first time to simultaneously map unconditional and conditional QTLs for tiller numbers at various stages by using single segment substitution lines in rice. Fourteen QTLs for tiller number, distributing on the corresponding substitution segments of chromosomes 1, 2, 3, 4, 6, 7 and 8 were detected. Both the number and the effect of the QTLs for tiller number were various at different stages, from 6 to 9 in the number and from 1.49 to 3.49 in the effect, respectively. Tiller number QTLs expressed in a time order, mainly detected at three stages of 0-7 d, 14-21 d and 35-42 d after transplanting with 6 positive, 9 random and 6 negative expressing QTLs, respectively. Each of the QTLs expressed one time at least during the whole duration of rice. The tiller number at a specific stage was determined by sum of QTL effects estimated by the unconditional method, while the increasing or decreasing number in a given time interval was controlled by the total of QTL effects estimated by the conditional method. These results demonstrated that it is highly effective and accurate for mapping of the QTLs by using single segment substitution lines and the conditional analysis methodology.展开更多
Background and Objective It has been proven that copy number gain/or loss (copy number variation CNV) in uences gene expression and result in phenotypic variation by
Using mapping method and topological current theory, the topological structure of disclination lines in 2 dimensional liquid crystals is studied. By introducing the strength density and the topological current of m...Using mapping method and topological current theory, the topological structure of disclination lines in 2 dimensional liquid crystals is studied. By introducing the strength density and the topological current of many disclination lines, it is pointed out that the disclination lines are determined by the singulaities of the director field, and topologically quantized by the Hopf indices and Brouwer degrees. Due to the equivalence in physics of the director fields n (x) and n (x) , the Hopf indices can be integers or half integers, representing a generalization of our previous studies of integer Hopf indices.展开更多
The electromagnetic wave growth or damping depends basically on the number density and anisotropy of energetic particles as the resonant interaction takes place between the particles and waves in the magnetosphere. Th...The electromagnetic wave growth or damping depends basically on the number density and anisotropy of energetic particles as the resonant interaction takes place between the particles and waves in the magnetosphere. The variance of both the number density and anisotropy along the magnetic field line is evaluated systematically by modeling four typically prescribed distribution functions. It is shown that in the case of ＂the positive anisotropy＂ （namely, the perpendicular temperature T⊥ exceeds the parallel temperature T｜｜）, the number density of energetic electrons always decreases with the magnetic latitude for a regular increasing magnetic field and the maximum wave growth is therefore generally confined to the equator where the resonant energy is minimum, and the number density is the largest. However, the ＂loss-cone＂ anisotropy of the electrons with a ＂pancake＂ distribution or kappa distribution keeps invariant or nearly invariant, whereas the ＂temperature＂ anisotropy with a pure bi-Maxwellian distribution or Ashour-Abdalla and Kennel＇s distributions decreases with the magnetic latitude. The results may provide a useful approach to evaluating the number density and anisotropy of the energetic electrons at latitudes where the observation information is not available.展开更多
Complex traits, such as yield components, are inherited in a quantitative manner and typically controlled by quantitative trait loci (QTL). Grain number per panicle (GN) is an important component of yield in rice and
An analysis is made of the problem of sound radiation from infinite one-dimensional plateson elastic foundation, when the plates are subjected to the action of harmonic line forces movingat subsonic speeds (M 【 1). T...An analysis is made of the problem of sound radiation from infinite one-dimensional plateson elastic foundation, when the plates are subjected to the action of harmonic line forces movingat subsonic speeds (M 【 1). The expressions of nondimensional sound power are formulated andthe asymptotic forms of sound power in the low frequency regions are derived. The radiatedsound power is shown as a function of the stiffness of elastic foundation, in terms of stiffness fac-torψ, the moving speed of line force, in terms of Math number M, and the frequency, in termsof wavenumber ratio γ . The effects of the parameter ψ in conjunction with the parameters Mand γ on the radiated sound power level and the phenomenon of coincidence radiation are alsoinvestigated in detail.展开更多
Unconditional and conditional QTL mapping were conducted for growth duration (GD), plant height (PH) and effective panicle number per plant (PN) using a recombinant inbred line (RIL) population derived from a cross be...Unconditional and conditional QTL mapping were conducted for growth duration (GD), plant height (PH) and effective panicle number per plant (PN) using a recombinant inbred line (RIL) population derived from a cross between two japonica rice varieties Xiushui 79 and C Bao. The RIL population consisted of 254 lines was planted in two environments, Nanjing and Sihong, Jiangsu Province, China. Results showed that additive effects were major in all of QTLs for GD, PH and PN detected by the two methods, and the epistatic effects explained a small proportion of phenotypic variation. No interactions were detected between additive QTL and environment, and between epistatic QTL pairs and environment. After growth duration was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.71. When effective panicle number per plant was adjusted to an identical level, RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 4.64. After plant height was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.62, and RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 3.89. These applicable elite alleles could be used to improve target traits without influencing the other two traits.展开更多
The line graph for the complement of the zero divisor graph for the ring of Gaussian integers modulo n is studied. The diameter, the radius and degree of each vertex are determined. Complete characterization of Hamilt...The line graph for the complement of the zero divisor graph for the ring of Gaussian integers modulo n is studied. The diameter, the radius and degree of each vertex are determined. Complete characterization of Hamiltonian, Eulerian, planer, regular, locally and locally connected is given. The chromatic number when is a power of a prime is computed. Further properties for and are also discussed.展开更多
基金supported by the National Key Research Plan Project (2017YFD0101107)the Chongqing Science and Technology Commission Special Project (cstc2016shmsztzx0032)the Southwest University Innovation Team Project (XDJK2017A004)
文摘A chromosome segment substitution line (CSSL) is a powerful tool for combining quantitative trait locus (QTL) mapping with the pyramiding of desirable alleles. The rice CSSL Z1364 with increased kernel number was identified in a BC3F8 population derived from a cross of Nipponbare as the recipient with Xihui 18 as the donor parent. Z1364 carried three substitution segments distributed on chromosomes 1, 6, and 8. The mean substitution length was 1.19 Mb. Of 17 QTL identified on the substitution segments, qSP1 for spikelets per panicle, qSSD1 for seed-set density, and qNSB1 for number of secondary branches explained respectively 57.34%, 87.7%, and 49.44% of the corresponding phenotypic variance and were all linked to RM6777. Chi-square analysis showed that the increased kernel number in Z1364 was inherited recessively by a single gene. By fine mapping, qSP1 was delimited to a 50-kb region on the short arm of chromosome 1. Based on DNA sequence, a previously uncharacterized rice homolog of Arabidopsis thaliana AT4G32551 was identified as a candidate gene for qSP1 in which mutation increases the number of spikelets and kernels in Z1364. qSP1 was expressed in all tissues, but particularly in 1-cm panicles. The expression levels of OsMADS22, GN1A, and DST were upregulated and those of LAX2, GNP1, and GHD7 were downregulated in Nipponbare. These results provide a foundation for functional research on qSP1.
基金supported by the National Key Research and Development Program of China (2017YFD0101004)the National Natural Science Foundation of China (91435204)the Science and Technology Independent Innovation Ability Upgrading Project of Shanxi Academy of Agricultural Sciences (2017ZZCX-23)
文摘Hexaploid triticale(×Triticosecale,AABBRR)is an important forage crop and a promising energy plant.Transferring D-genome chromosomes or segments from common wheat(Triticum aestivum)into hexaploid triticale is attractive in improving its economically important traits.Here,a hexaploid triticale 6D(6A)substitution line Lin 456 derived from the cross between the octoploid triticale line H400 and the hexaploid wheat Lin 56 was identified and analyzed by genomic in situ hybridization(GISH),fluorescence in situ hybridization(FISH),and molecular markers.The GISH analysis showed that Lin 456 is a hexaploid triticalewith 14 rye(Secale cereale)chromosomes and 28 wheat chromosomes,whereas non-denaturing fluorescence in situ hybridization(ND-FISH)and molecular marker analysis revealed that it is a 6D(6A)substitution line.In contrast to previous studies,the signal of Oligo-pSc119.2 was observed at the distal end of 6DL in Lin 456.The wheat chromosome 6D was associatedwith increased grain weight and decreased spikelet number using the genotypic data combined with the phenotypes of the F2 population in the three environments.The thousand-grain weight and grain width in the substitution individuals were significantly higher than those in the non-substitution individuals in the F2 population across the three environments.We propose that the hexaploid triticale 6D(6A)substitution line Lin 456 can be a valuable and promising donor stock for genetic improvement during triticale breeding.
文摘Following NCI design, the developmental genetic behavior of tiller number (TN) in three-line indica hybrid rice was studied using additive-dominance developmental genetic models and the corresponding statistical methods. The results showed that dominance effects were predominant for TN. The expression of those additive effects were affected by environment and genotype interaction, but the expression of dominance effects were not affected. Heterosis was the strongest in the middle developmental periods of TN. Additive effects and dominance effects were selectively expressed throughout in the entire tillering developmental stage. Analysis of genetic correlation between TN at different stages and the productive panicles indicated that a close correlation appeared earlier in the populations with higher heterosis than in those with less heterosis. Utilization of heterosis at the middle tillering stage might enhance the final biomass but reduce the percentage of productive panicles.
基金supported by the grants from the National.Basic Research Program of China(2006CB 101700)the National Natural Science Foundation of China(30330370).
文摘Tiller is one of the most important agronomic traits which influences quantity and quality of effective panicles and finally influences yield in rice. It is important to understand ＂static＂ and ＂dynamic＂ information of the QTLs for tillers in rice. This work was the first time to simultaneously map unconditional and conditional QTLs for tiller numbers at various stages by using single segment substitution lines in rice. Fourteen QTLs for tiller number, distributing on the corresponding substitution segments of chromosomes 1, 2, 3, 4, 6, 7 and 8 were detected. Both the number and the effect of the QTLs for tiller number were various at different stages, from 6 to 9 in the number and from 1.49 to 3.49 in the effect, respectively. Tiller number QTLs expressed in a time order, mainly detected at three stages of 0-7 d, 14-21 d and 35-42 d after transplanting with 6 positive, 9 random and 6 negative expressing QTLs, respectively. Each of the QTLs expressed one time at least during the whole duration of rice. The tiller number at a specific stage was determined by sum of QTL effects estimated by the unconditional method, while the increasing or decreasing number in a given time interval was controlled by the total of QTL effects estimated by the conditional method. These results demonstrated that it is highly effective and accurate for mapping of the QTLs by using single segment substitution lines and the conditional analysis methodology.
基金supported by a grant from the key project of the National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30430300)National Natural Science Foundation of China (to Qinghua ZHOU)(No. 30670922)INTERNATION Scienc and Techniquie COOPRATION PROGRAM OF CHINA (ISCP) (to Qinghua ZHOU)(No.2006DFB32330)
文摘Background and Objective It has been proven that copy number gain/or loss (copy number variation CNV) in uences gene expression and result in phenotypic variation by
文摘Using mapping method and topological current theory, the topological structure of disclination lines in 2 dimensional liquid crystals is studied. By introducing the strength density and the topological current of many disclination lines, it is pointed out that the disclination lines are determined by the singulaities of the director field, and topologically quantized by the Hopf indices and Brouwer degrees. Due to the equivalence in physics of the director fields n (x) and n (x) , the Hopf indices can be integers or half integers, representing a generalization of our previous studies of integer Hopf indices.
基金supported by National Natural Science Foundation of China (Nos. 40474064, 40404012)the Scientific Research Foundation for ROCS, SEMthe Outstanding Youth Foundation of the Education Bureau of Hunan Province (No. 04B003)
文摘The electromagnetic wave growth or damping depends basically on the number density and anisotropy of energetic particles as the resonant interaction takes place between the particles and waves in the magnetosphere. The variance of both the number density and anisotropy along the magnetic field line is evaluated systematically by modeling four typically prescribed distribution functions. It is shown that in the case of ＂the positive anisotropy＂ （namely, the perpendicular temperature T⊥ exceeds the parallel temperature T｜｜）, the number density of energetic electrons always decreases with the magnetic latitude for a regular increasing magnetic field and the maximum wave growth is therefore generally confined to the equator where the resonant energy is minimum, and the number density is the largest. However, the ＂loss-cone＂ anisotropy of the electrons with a ＂pancake＂ distribution or kappa distribution keeps invariant or nearly invariant, whereas the ＂temperature＂ anisotropy with a pure bi-Maxwellian distribution or Ashour-Abdalla and Kennel＇s distributions decreases with the magnetic latitude. The results may provide a useful approach to evaluating the number density and anisotropy of the energetic electrons at latitudes where the observation information is not available.
文摘Complex traits, such as yield components, are inherited in a quantitative manner and typically controlled by quantitative trait loci (QTL). Grain number per panicle (GN) is an important component of yield in rice and
文摘An analysis is made of the problem of sound radiation from infinite one-dimensional plateson elastic foundation, when the plates are subjected to the action of harmonic line forces movingat subsonic speeds (M 【 1). The expressions of nondimensional sound power are formulated andthe asymptotic forms of sound power in the low frequency regions are derived. The radiatedsound power is shown as a function of the stiffness of elastic foundation, in terms of stiffness fac-torψ, the moving speed of line force, in terms of Math number M, and the frequency, in termsof wavenumber ratio γ . The effects of the parameter ψ in conjunction with the parameters Mand γ on the radiated sound power level and the phenomenon of coincidence radiation are alsoinvestigated in detail.
基金supported by the Program of National High Technology Research and Development, Ministry of Science and Technology, China (Grant No. 2010AA101301)the Program of Introducing Talents of Discipline to University in China (Grant No. B08025)+1 种基金the Program of Introducing International Advanced Agricultural Science and Technology in China (Grant No. 2006-G8 [4]-31-1) the Program of Science-Technology Basis and Conditional Platform in China (Grant No. 505005)
文摘Unconditional and conditional QTL mapping were conducted for growth duration (GD), plant height (PH) and effective panicle number per plant (PN) using a recombinant inbred line (RIL) population derived from a cross between two japonica rice varieties Xiushui 79 and C Bao. The RIL population consisted of 254 lines was planted in two environments, Nanjing and Sihong, Jiangsu Province, China. Results showed that additive effects were major in all of QTLs for GD, PH and PN detected by the two methods, and the epistatic effects explained a small proportion of phenotypic variation. No interactions were detected between additive QTL and environment, and between epistatic QTL pairs and environment. After growth duration was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.71. When effective panicle number per plant was adjusted to an identical level, RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 4.64. After plant height was adjusted to an identical level, RM80-160bp was detected as an applicable elite allele for PN, with an additive effect of 0.62, and RM448-240bp was detected as an applicable elite allele for GD, with an additive effect of 3.89. These applicable elite alleles could be used to improve target traits without influencing the other two traits.
文摘The line graph for the complement of the zero divisor graph for the ring of Gaussian integers modulo n is studied. The diameter, the radius and degree of each vertex are determined. Complete characterization of Hamiltonian, Eulerian, planer, regular, locally and locally connected is given. The chromatic number when is a power of a prime is computed. Further properties for and are also discussed.
