Nuclear factor Y is a ubiquitous heterotrimeric transcription factor complex conserved across eukaryotes that binds to CCAAT boxes,one of the most common motifs found in gene promoters and enhancers.Over the last 30 y...Nuclear factor Y is a ubiquitous heterotrimeric transcription factor complex conserved across eukaryotes that binds to CCAAT boxes,one of the most common motifs found in gene promoters and enhancers.Over the last 30 years,research has revealed that the nuclear factor Y complex controls many aspects of brain development,including differentiation,axon guidance,homeostasis,disease,and most recently regeneration.However,a complete understanding of transcriptional regulatory networks,including how the nuclear factor Y complex binds to specific CCAAT boxes to perform its function remains elusive.In this review,we explore the nuclear factor Y complex’s role and mode of action during brain development,as well as how genomic technologies may expand understanding of this key regulator of gene expression.展开更多
The timing of floral transition is critical for reproductive success in flowering plants.In long-day(LD)plant Arabidopsis,the floral regulator gene FLOWERING LOCUS T(FT)is a major component of the mobile florigen.FT e...The timing of floral transition is critical for reproductive success in flowering plants.In long-day(LD)plant Arabidopsis,the floral regulator gene FLOWERING LOCUS T(FT)is a major component of the mobile florigen.FT expression is rhythmically activated by CONSTANS(CO),and specifically accumu-lated at dusk of LDs.However;the underlying mechanism of adequate regulation of FT transcription in response to day-length cues to warrant flowering time still remains to be investigated.Here,we identify a homolog of human protein arginine methyltransferases 6(HsPRMT6)in Arabidopsis,and confirm AtPRMT6 physically interacts with three positive regulators of flowering Nuclear Factors YC3(NF-YC3),NF-YC9,and NF-YB3.Further investigations find that AtPRMT6 and its encoding protein accumulate at dusk of LDs.PRMT6-mediated H3 R2me2a modification enhances the promotion of NF-YCs on FT transcription in response to inductive LD signals.Moreover,AtPRMT6 and its homologues proteins AtPRMT4a and AtPRMT4b coordinately inhibit the expression of FLOWERING LOCUS C,a suppressor of FT.Taken together,our study reveals the role of arginine methylation in photoperiodic pathway and how the PRMT6-mediating H3R2me2a system interacts with NF-CO module to dynamically control FT expression and facilitate flowering time.展开更多
The nuclear factor Y(NF-Y)is a class of heterotrimeric transcription factors comprising three subunits:NF-YA,NF-YB,and NF-YC.These transcription factors participate in many plant bioprocesses,including the regulation ...The nuclear factor Y(NF-Y)is a class of heterotrimeric transcription factors comprising three subunits:NF-YA,NF-YB,and NF-YC.These transcription factors participate in many plant bioprocesses,including the regulation of flowering time.Although the NF-Y gene family has been systematically studied in many species,little is known about its role in the non-heading Chinese cabbage(NHCC)[Brassica campestris(syn.Brassica rapa)ssp.chinensis].In this study,we identified 57 NF-Y members in the genome of NHCC using BLASTP,including 20 BcNF-YAs,24BcNF-YBs,and 13 BcNF-YCs.These genes are randomly distributed on the 10 chromosomes of NHCC.The results of yeast two-hybrid experiments indicated that among some members of the three subunits of BcNF-Ys,the members of the NF-YA and NF-YC subunits interact with each other,a third of the members of the NF-YB and NF-YC subunits interact with each other,while no interaction was observed between the members of the NF-YA and NF-YB subunits.Subcellular localization experiments in tobacco showed that Bc NF-YA2 and BcNF-YA8 were expressed in the nucleus;BcNF-YB18 and BcNF-YB23 were located in the cell membrane and cytoplasm;and BcNF-YC6 and BcNF-YC7 were expressed in the nucleus,cytoplasm,and cell membrane.We analyzed the cis-acting elements in the promoter of BcNF-Y genes and found that the ABA response element is the most distributed hormone response element,which is regulated by ABA signals triggered by environmental stimuli.Accordingly,we treated three-week-old NHCC leaves with 100μmol L^(-1) ABA and analyzed the expression profile of BcNF-Ys through RNA-seq.The results showed that except for six undetected BcNF-Ys,the remaining 51 BcNF-Ys showed varying degrees of response to ABA signals.Among these,BcNF-YA8 was positively regulated by ABA signals,with the highest upregulation amplitude.Subsequently,the function of BcNF-YA8 was extensively studied,which demonstrated that its expression promotes plant flowering.This result enriches our understanding of the potential molecular mechanism by which ABA positively regulates NHCC flowering.展开更多
Nuclear factor Y(NF-Y) is a ubiquitous transcription factor that regulates important physiological and developmental processes. In this study, we identified 34 Os NF-Y genes in rice, including 6 newly identified genes...Nuclear factor Y(NF-Y) is a ubiquitous transcription factor that regulates important physiological and developmental processes. In this study, we identified 34 Os NF-Y genes in rice, including 6 newly identified genes. Expression profile analysis covering the whole life cycle revealed that transcripts of Os NF-Y differentially accumulated in a tissue-specific,preferential or constitutive manner. In addition, gene duplication studies and expression analyses were performed to determine the evolutionary origins of the Os NF-Y gene family.Nine Os NF-Y genes were differentially expressed after treatment of seedlings with one or more abiotic stresses such as drought, salt and cold. Analysis of expression correlation and Gene Ontology annotation suggested that Os NF-Y genes were co-expressed with genes that participated in stress, accumulation of seed storage reserves, and plant development.Co-expression analysis also revealed that Os NF-Y genes might interact with each other,suggesting that NF-Y subunits formed complexes that take part in transcriptional regulation. These results provide useful information for further elucidating the function of the NF-Y family and their regulatory pathways.展开更多
探讨核因子Y(nuclear factor Y,NFY)和调节因子X1(regulatory factors that bind to the X box,RFX1)对人PNRC(proline-rich nuclear receptor coactivator)基因的调控作用及机制.根据凝胶电泳迁移率变化实验,分析NFY和RFX1与PNRC启动...探讨核因子Y(nuclear factor Y,NFY)和调节因子X1(regulatory factors that bind to the X box,RFX1)对人PNRC(proline-rich nuclear receptor coactivator)基因的调控作用及机制.根据凝胶电泳迁移率变化实验,分析NFY和RFX1与PNRC启动子区域的结合.将含有NFY和RFX1的真核表达质粒(pCMV-NFY,pCMV-RFX1)和含有PNRC启动子的荧光素酶报告基因质粒共转染HepG2细胞,检测转染细胞的荧光素酶活性,并用RT-PCR和Western印迹检测PNRC的表达情况.Quick-Change法对PNRC启动子区NFY和RFX1结合位点进行突变,将包含突变点的重组荧光素酶报告质粒与含有NFY和RFX1的真核表达质粒共同转染HepG2细胞,检测各组荧光素酶活性.结果发现,NFY和RFX1能与PNRC启动子区域特异性结合;转染pCMV-NFY和pCMV-RFX1可抑制PNRC启动子活性并下调PNRC在HepG2细胞中的表达;包含NFY和RFX1结合位点的突变质粒与pCMV-NFY和pCMV-RFX1共转染后,NFY和RFX1对PNRC启动子活性的抑制作用消失.以上结果提示,NFY和RFX1能调控PNRC基因的表达,其机制是与PNRC启动子区域的特异性结合位点相结合,发挥其反式抑制作用.展开更多
目的核转录因子Y的C亚基的反义RNA 1(nuclear transcription factor Y subunit C antisense RNA 1,NFYC⁃AS1)可能是肺腺癌的预后标志物。然而,NFYC⁃AS1的具体作用机制尚不明确,该研究拟探讨NFYC⁃AS1在肺癌细胞系中的作用机制。方法在H83...目的核转录因子Y的C亚基的反义RNA 1(nuclear transcription factor Y subunit C antisense RNA 1,NFYC⁃AS1)可能是肺腺癌的预后标志物。然而,NFYC⁃AS1的具体作用机制尚不明确,该研究拟探讨NFYC⁃AS1在肺癌细胞系中的作用机制。方法在H838细胞系中分别建立NFYC⁃AS1敲除和对照细胞株,然后进行WST⁃1/侵袭和转移/蛋白质印迹法(western blotting)实验,验证NFYC⁃AS1的功能。结果下调NFYC⁃AS1后,H838细胞系的增殖、迁移和侵袭受到抑制,自噬主要蛋白P62下调,Becline上调,同时MET和c⁃Myc蛋白的表达下调。结论NFYC⁃AS1可能在H838细胞系中经促增殖移动、抑制自噬而发挥促癌作用。展开更多
The identification of seed development-related regulators is critical for the genetic improvement of yield and grain quality in cereal crops.SQUAMOSA PROMOTER BINDING PROTEIN-LIKE14(OsSPL14)is a well-studied,plant-spe...The identification of seed development-related regulators is critical for the genetic improvement of yield and grain quality in cereal crops.SQUAMOSA PROMOTER BINDING PROTEIN-LIKE14(OsSPL14)is a well-studied,plant-specific transcription factor;however,its roles in controlling rice grain appearance quality and the underlying molecular mechanisms have not been fully elucidated.In this study,we demon-strate that OsSPL14 positively regulates appearance quality by controlling grain chalkiness in rice.Genetic analysis revealed that knockdown or knockout of OsSPL14 leads to a chalky grain phenotype,which is associated with significant defects in compound starch granules and notable changes in both starch and protein contents in the endosperm.Transcript analysis identified multiple genes regulated by OsSPL14,including the key granule-bound starch synthase gene Waxy(Wx)and the protein disulfide isomerase-like enzyme-encoding gene PDIL1-1.Both in vitro and in vivo assays demonstrated that OsSPL14 directly binds to the GTAC-box motif in the Wx and PDIL1-1 promoters to enhance their expression.Protein-protein inter-action experiments further revealed that OsSPL14 interacts with the nuclear transcription factor Y(NF-Y)heterodimer OsNF-YB9/YC8-12 to promote the transcription of Wx and PDIL1-1,thereby enhancing rice grain appearance quality.Our findings uncover a novel regulatory pathway controlled by OsSPL14 and pro-vide new insights into the molecular mechanisms underlying rice grain appearance quality,with promising implications for genetic improvement in rice.展开更多
基金supported by National Health and Medical Research Council GNT1105374,GNT1137645,GNT2000766 and veski Innovation Fellowship(VIF23)to RP.
文摘Nuclear factor Y is a ubiquitous heterotrimeric transcription factor complex conserved across eukaryotes that binds to CCAAT boxes,one of the most common motifs found in gene promoters and enhancers.Over the last 30 years,research has revealed that the nuclear factor Y complex controls many aspects of brain development,including differentiation,axon guidance,homeostasis,disease,and most recently regeneration.However,a complete understanding of transcriptional regulatory networks,including how the nuclear factor Y complex binds to specific CCAAT boxes to perform its function remains elusive.In this review,we explore the nuclear factor Y complex’s role and mode of action during brain development,as well as how genomic technologies may expand understanding of this key regulator of gene expression.
基金the Natural National Science Foundation of China(32101786)the National Transgenic Major Program(2019ZX08010-002)+1 种基金the Fu ndamental Research Funds for Central Non-profit Scientific Institution(1610392017001)the Baichuan Project at the College of Life Science and Technology,Huazhong Agricultural University.
文摘The timing of floral transition is critical for reproductive success in flowering plants.In long-day(LD)plant Arabidopsis,the floral regulator gene FLOWERING LOCUS T(FT)is a major component of the mobile florigen.FT expression is rhythmically activated by CONSTANS(CO),and specifically accumu-lated at dusk of LDs.However;the underlying mechanism of adequate regulation of FT transcription in response to day-length cues to warrant flowering time still remains to be investigated.Here,we identify a homolog of human protein arginine methyltransferases 6(HsPRMT6)in Arabidopsis,and confirm AtPRMT6 physically interacts with three positive regulators of flowering Nuclear Factors YC3(NF-YC3),NF-YC9,and NF-YB3.Further investigations find that AtPRMT6 and its encoding protein accumulate at dusk of LDs.PRMT6-mediated H3 R2me2a modification enhances the promotion of NF-YCs on FT transcription in response to inductive LD signals.Moreover,AtPRMT6 and its homologues proteins AtPRMT4a and AtPRMT4b coordinately inhibit the expression of FLOWERING LOCUS C,a suppressor of FT.Taken together,our study reveals the role of arginine methylation in photoperiodic pathway and how the PRMT6-mediating H3R2me2a system interacts with NF-CO module to dynamically control FT expression and facilitate flowering time.
基金supported by the National Natural Science Foundation of China(Grant No.31872106)the National Vegetable Industry Technology System(Grant No.CARS-23-A-16)+1 种基金the Jiangsu Seed Industry Revitalization Project(Grant No.JBGS(2021)015)the Priority Academic Program Development of Jiangsu Higher Education Institutions。
文摘The nuclear factor Y(NF-Y)is a class of heterotrimeric transcription factors comprising three subunits:NF-YA,NF-YB,and NF-YC.These transcription factors participate in many plant bioprocesses,including the regulation of flowering time.Although the NF-Y gene family has been systematically studied in many species,little is known about its role in the non-heading Chinese cabbage(NHCC)[Brassica campestris(syn.Brassica rapa)ssp.chinensis].In this study,we identified 57 NF-Y members in the genome of NHCC using BLASTP,including 20 BcNF-YAs,24BcNF-YBs,and 13 BcNF-YCs.These genes are randomly distributed on the 10 chromosomes of NHCC.The results of yeast two-hybrid experiments indicated that among some members of the three subunits of BcNF-Ys,the members of the NF-YA and NF-YC subunits interact with each other,a third of the members of the NF-YB and NF-YC subunits interact with each other,while no interaction was observed between the members of the NF-YA and NF-YB subunits.Subcellular localization experiments in tobacco showed that Bc NF-YA2 and BcNF-YA8 were expressed in the nucleus;BcNF-YB18 and BcNF-YB23 were located in the cell membrane and cytoplasm;and BcNF-YC6 and BcNF-YC7 were expressed in the nucleus,cytoplasm,and cell membrane.We analyzed the cis-acting elements in the promoter of BcNF-Y genes and found that the ABA response element is the most distributed hormone response element,which is regulated by ABA signals triggered by environmental stimuli.Accordingly,we treated three-week-old NHCC leaves with 100μmol L^(-1) ABA and analyzed the expression profile of BcNF-Ys through RNA-seq.The results showed that except for six undetected BcNF-Ys,the remaining 51 BcNF-Ys showed varying degrees of response to ABA signals.Among these,BcNF-YA8 was positively regulated by ABA signals,with the highest upregulation amplitude.Subsequently,the function of BcNF-YA8 was extensively studied,which demonstrated that its expression promotes plant flowering.This result enriches our understanding of the potential molecular mechanism by which ABA positively regulates NHCC flowering.
基金supported by the National Natural Science Foundation of China(Nos.31570321,30971551)
文摘Nuclear factor Y(NF-Y) is a ubiquitous transcription factor that regulates important physiological and developmental processes. In this study, we identified 34 Os NF-Y genes in rice, including 6 newly identified genes. Expression profile analysis covering the whole life cycle revealed that transcripts of Os NF-Y differentially accumulated in a tissue-specific,preferential or constitutive manner. In addition, gene duplication studies and expression analyses were performed to determine the evolutionary origins of the Os NF-Y gene family.Nine Os NF-Y genes were differentially expressed after treatment of seedlings with one or more abiotic stresses such as drought, salt and cold. Analysis of expression correlation and Gene Ontology annotation suggested that Os NF-Y genes were co-expressed with genes that participated in stress, accumulation of seed storage reserves, and plant development.Co-expression analysis also revealed that Os NF-Y genes might interact with each other,suggesting that NF-Y subunits formed complexes that take part in transcriptional regulation. These results provide useful information for further elucidating the function of the NF-Y family and their regulatory pathways.
文摘探讨核因子Y(nuclear factor Y,NFY)和调节因子X1(regulatory factors that bind to the X box,RFX1)对人PNRC(proline-rich nuclear receptor coactivator)基因的调控作用及机制.根据凝胶电泳迁移率变化实验,分析NFY和RFX1与PNRC启动子区域的结合.将含有NFY和RFX1的真核表达质粒(pCMV-NFY,pCMV-RFX1)和含有PNRC启动子的荧光素酶报告基因质粒共转染HepG2细胞,检测转染细胞的荧光素酶活性,并用RT-PCR和Western印迹检测PNRC的表达情况.Quick-Change法对PNRC启动子区NFY和RFX1结合位点进行突变,将包含突变点的重组荧光素酶报告质粒与含有NFY和RFX1的真核表达质粒共同转染HepG2细胞,检测各组荧光素酶活性.结果发现,NFY和RFX1能与PNRC启动子区域特异性结合;转染pCMV-NFY和pCMV-RFX1可抑制PNRC启动子活性并下调PNRC在HepG2细胞中的表达;包含NFY和RFX1结合位点的突变质粒与pCMV-NFY和pCMV-RFX1共转染后,NFY和RFX1对PNRC启动子活性的抑制作用消失.以上结果提示,NFY和RFX1能调控PNRC基因的表达,其机制是与PNRC启动子区域的特异性结合位点相结合,发挥其反式抑制作用.
基金supported by grants from the National Natural Science Foundation of China(32472141 and 32101746)the Natural Science Foundation of Hubei Province(JCZRLH202500540,2021CFB033).
文摘The identification of seed development-related regulators is critical for the genetic improvement of yield and grain quality in cereal crops.SQUAMOSA PROMOTER BINDING PROTEIN-LIKE14(OsSPL14)is a well-studied,plant-specific transcription factor;however,its roles in controlling rice grain appearance quality and the underlying molecular mechanisms have not been fully elucidated.In this study,we demon-strate that OsSPL14 positively regulates appearance quality by controlling grain chalkiness in rice.Genetic analysis revealed that knockdown or knockout of OsSPL14 leads to a chalky grain phenotype,which is associated with significant defects in compound starch granules and notable changes in both starch and protein contents in the endosperm.Transcript analysis identified multiple genes regulated by OsSPL14,including the key granule-bound starch synthase gene Waxy(Wx)and the protein disulfide isomerase-like enzyme-encoding gene PDIL1-1.Both in vitro and in vivo assays demonstrated that OsSPL14 directly binds to the GTAC-box motif in the Wx and PDIL1-1 promoters to enhance their expression.Protein-protein inter-action experiments further revealed that OsSPL14 interacts with the nuclear transcription factor Y(NF-Y)heterodimer OsNF-YB9/YC8-12 to promote the transcription of Wx and PDIL1-1,thereby enhancing rice grain appearance quality.Our findings uncover a novel regulatory pathway controlled by OsSPL14 and pro-vide new insights into the molecular mechanisms underlying rice grain appearance quality,with promising implications for genetic improvement in rice.
