Mesenchymal stem cell(MSC)-derived exosomes(Exos)were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect.MSCs pretreated with chemistry or biology factors wer...Mesenchymal stem cell(MSC)-derived exosomes(Exos)were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect.MSCs pretreated with chemistry or biology factors were reported to advance the biological activities of MSC-derived exosomes.Hence,this study was designed to explore whether exosomes derived from human umbilical cord MSCs(hucMSCs)preconditioned with Nocardia rubra cell wall skeleton(Nr-CWS)exhibited superior proangiogenic effect on diabetic wound repair and its underlying molecular mechanisms.The results showed that Nr-CWS-Exos facilitated the proliferation,migration and tube formation of endothelial cells in vitro.In vivo,Nr-CWS-Exos exerted great effect on advancing wound healing by facilitating the angiogenesis of wound tissues compared with Exos.Furthermore,the expression of circIARS1 increased after HUVECs were treated with Nr-CWS-Exos.CircIARS1 promoted the pro-angiogenic effects of Nr-CWS-Exos on endothelial cells via the miR-4782-5p/VEGFA axis.Taken together,those data reveal that exosomes derived from Nr-CWS-pretreated MSCs might serve as an underlying strategy for diabetic wound treatment through advancing the biological function of endothelial cells via the circIARS1/miR-4782-5p/VEGFA axis.展开更多
基金the National Natural Science Foundation of Jiangsu Province(No.BK20201155)the Foundation of Xuzhou Key Medical Talents(No.XWRCHT-20220067).
文摘Mesenchymal stem cell(MSC)-derived exosomes(Exos)were reported to a prospective candidate in accelerating diabetic wound healing due to their pro-angiogenic effect.MSCs pretreated with chemistry or biology factors were reported to advance the biological activities of MSC-derived exosomes.Hence,this study was designed to explore whether exosomes derived from human umbilical cord MSCs(hucMSCs)preconditioned with Nocardia rubra cell wall skeleton(Nr-CWS)exhibited superior proangiogenic effect on diabetic wound repair and its underlying molecular mechanisms.The results showed that Nr-CWS-Exos facilitated the proliferation,migration and tube formation of endothelial cells in vitro.In vivo,Nr-CWS-Exos exerted great effect on advancing wound healing by facilitating the angiogenesis of wound tissues compared with Exos.Furthermore,the expression of circIARS1 increased after HUVECs were treated with Nr-CWS-Exos.CircIARS1 promoted the pro-angiogenic effects of Nr-CWS-Exos on endothelial cells via the miR-4782-5p/VEGFA axis.Taken together,those data reveal that exosomes derived from Nr-CWS-pretreated MSCs might serve as an underlying strategy for diabetic wound treatment through advancing the biological function of endothelial cells via the circIARS1/miR-4782-5p/VEGFA axis.
